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The map label for this gene is pleD [H]

Identifier: 225630041

GI number: 225630041

Start: 209725

End: 211101

Strand: Reverse

Name: pleD [H]

Synonym: WRi_002100

Alternate gene names: 225630041

Gene position: 211101-209725 (Counterclockwise)

Preceding gene: 225630042

Following gene: 225630038

Centisome position: 14.6

GC content: 35.08

Gene sequence:

>1377_bases
ATGACAGCGAAGATTCTGGTAGTAGATGATGTACTATCTAATGTCAAGCTTTTAGAAGCTCGACTAAAAGCAGAGTACTA
TACGGTTATCGTAGCTCATGATGGTGAAGAAGCTATAGATTTAGTAGCAAAGCAACAGCCTGATATTATATTACTTGATA
TCATGATGCCAAAGATGAATGGCTTTCAAGTTTGTAAGGAGCTAAAGAGTGATCCCTTGACAACCCATATTCCAATAATT
ATGGTAACTGCACTGCATGACACTCATGATAGAGTGCAAGGTATTAATGCTGGCGCGGATGATTTTCTGACTAAACCAAT
AAACGAAACTGCTTTATCTGCACGAATTAAGTCTCTCACGCGCTTAAAAATGATTATAGATGAATTGCGTTTAAGAGGAG
AAACCAACGCTGAGATTGGCGGAGTAGCAGGAAACAGTATTATGGATTATTCCAATCAGATTTTTGATGCTAACATACTT
GTTGTAGATGAGGACGTCTTTCAGGCAGAGCAAATATATAATGTACTAAAGCAACGCTTTAGGTCAATTAAAATACTGAG
TGATCCAATAGAGGCATTAAAGGTTGGTATTAAGGATAATTACGACTTGATCATTTCTGATATGCAGTTTTCCAAAACTG
ACGGCTTGCGTTTGTGTTCTGGGTTTCGTAGTAAGGTGGAAACACGTTATACACCAATTCTAATTCTTTCTGAGGATTAT
GATAAAAACAATTTAGTAAAAGCACTTGATGTAGGTGCTAATGATTATTTAACAGTACCTTTGGATGAGGGTGAGTTAAT
AGCAAGGGTTAATTCGCAAGTAAAACGCAAAAGATATCAAGATGCCTTGAGAATGAATTTATTCAATAATGCGGAAATGT
CTATAAAGGACCCATTAACTAACTGTTATAATAGAAGATATTTTGATGCACACTTAAGAAACATTGTTAAGGACTCTGTA
GAAAAAGATAGAAGGTTGTCTCTTATGATACTTGATATAGACTATTTTAAGATGGTGAATGACAATTTTGGACATAGTGC
TGGAGATGAACTTTTAAAGCAAATACAGAGAAGAATTTCTGAAAATATTAGAGTGACAGATTTGTTAGCTAGATTTGGTG
GTGAGGAATTTGTTGTTGTAATGCCAGATACCGATACATCAGATGCGTATACTATTGCGGAAAGAATACGCAAAATTATT
GCTAAAAAACCTTTTATACTTGCGGATAAAAATACAACCCATAATGTAACTGTGAGCATTGGAATTGCAGAAATGCAAGG
ATCTGACCTTGACAATATTAAAGAATTTATAGTACGTGCCGACAAATATTTATATAAAGCAAAAAACAGTGGTAGAAATA
GAGTGGTTACTGGCTAG

Upstream 100 bases:

>100_bases
GACTTATATAAAGAGTGTAATACTGAGAAAGTAAATTACATAGTTGGTAAAAATGGTAAGCTTTATCCTACTTATGATAT
AGATAATACATCTAAAGATA

Downstream 100 bases:

>100_bases
ATCTCTGAAATTAGACATATAGCTAAAGCAATGTATGTTTACAGATAGTGCAATTGTACTAATGTTGTGATTTGAGGGCA
GTCTCCACCAGGAGGGTGTC

Product: response regulator PleD

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 458; Mature: 457

Protein sequence:

>458_residues
MTAKILVVDDVLSNVKLLEARLKAEYYTVIVAHDGEEAIDLVAKQQPDIILLDIMMPKMNGFQVCKELKSDPLTTHIPII
MVTALHDTHDRVQGINAGADDFLTKPINETALSARIKSLTRLKMIIDELRLRGETNAEIGGVAGNSIMDYSNQIFDANIL
VVDEDVFQAEQIYNVLKQRFRSIKILSDPIEALKVGIKDNYDLIISDMQFSKTDGLRLCSGFRSKVETRYTPILILSEDY
DKNNLVKALDVGANDYLTVPLDEGELIARVNSQVKRKRYQDALRMNLFNNAEMSIKDPLTNCYNRRYFDAHLRNIVKDSV
EKDRRLSLMILDIDYFKMVNDNFGHSAGDELLKQIQRRISENIRVTDLLARFGGEEFVVVMPDTDTSDAYTIAERIRKII
AKKPFILADKNTTHNVTVSIGIAEMQGSDLDNIKEFIVRADKYLYKAKNSGRNRVVTG

Sequences:

>Translated_458_residues
MTAKILVVDDVLSNVKLLEARLKAEYYTVIVAHDGEEAIDLVAKQQPDIILLDIMMPKMNGFQVCKELKSDPLTTHIPII
MVTALHDTHDRVQGINAGADDFLTKPINETALSARIKSLTRLKMIIDELRLRGETNAEIGGVAGNSIMDYSNQIFDANIL
VVDEDVFQAEQIYNVLKQRFRSIKILSDPIEALKVGIKDNYDLIISDMQFSKTDGLRLCSGFRSKVETRYTPILILSEDY
DKNNLVKALDVGANDYLTVPLDEGELIARVNSQVKRKRYQDALRMNLFNNAEMSIKDPLTNCYNRRYFDAHLRNIVKDSV
EKDRRLSLMILDIDYFKMVNDNFGHSAGDELLKQIQRRISENIRVTDLLARFGGEEFVVVMPDTDTSDAYTIAERIRKII
AKKPFILADKNTTHNVTVSIGIAEMQGSDLDNIKEFIVRADKYLYKAKNSGRNRVVTG
>Mature_457_residues
TAKILVVDDVLSNVKLLEARLKAEYYTVIVAHDGEEAIDLVAKQQPDIILLDIMMPKMNGFQVCKELKSDPLTTHIPIIM
VTALHDTHDRVQGINAGADDFLTKPINETALSARIKSLTRLKMIIDELRLRGETNAEIGGVAGNSIMDYSNQIFDANILV
VDEDVFQAEQIYNVLKQRFRSIKILSDPIEALKVGIKDNYDLIISDMQFSKTDGLRLCSGFRSKVETRYTPILILSEDYD
KNNLVKALDVGANDYLTVPLDEGELIARVNSQVKRKRYQDALRMNLFNNAEMSIKDPLTNCYNRRYFDAHLRNIVKDSVE
KDRRLSLMILDIDYFKMVNDNFGHSAGDELLKQIQRRISENIRVTDLLARFGGEEFVVVMPDTDTSDAYTIAERIRKIIA
KKPFILADKNTTHNVTVSIGIAEMQGSDLDNIKEFIVRADKYLYKAKNSGRNRVVTG

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1787802, Length=194, Percent_Identity=37.6288659793814, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1787262, Length=161, Percent_Identity=42.8571428571429, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1786584, Length=190, Percent_Identity=33.6842105263158, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI87082007, Length=162, Percent_Identity=35.8024691358025, Blast_Score=110, Evalue=3e-25,
Organism=Escherichia coli, GI87081881, Length=162, Percent_Identity=38.2716049382716, Blast_Score=105, Evalue=8e-24,
Organism=Escherichia coli, GI1788381, Length=159, Percent_Identity=35.8490566037736, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI145693134, Length=159, Percent_Identity=33.9622641509434, Blast_Score=99, Evalue=7e-22,
Organism=Escherichia coli, GI1788085, Length=167, Percent_Identity=35.3293413173653, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1786599, Length=121, Percent_Identity=38.0165289256198, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1788394, Length=189, Percent_Identity=30.6878306878307, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI2367329, Length=118, Percent_Identity=47.4576271186441, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI1789809, Length=118, Percent_Identity=37.2881355932203, Blast_Score=82, Evalue=9e-17,
Organism=Escherichia coli, GI1787816, Length=165, Percent_Identity=32.1212121212121, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1790860, Length=100, Percent_Identity=34, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1788956, Length=172, Percent_Identity=30.2325581395349, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI87082012, Length=118, Percent_Identity=33.0508474576271, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1786784, Length=118, Percent_Identity=34.7457627118644, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1790437, Length=113, Percent_Identity=35.3982300884956, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1788550, Length=151, Percent_Identity=29.8013245033113, Blast_Score=72, Evalue=9e-14,
Organism=Escherichia coli, GI1787541, Length=148, Percent_Identity=31.7567567567568, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787229, Length=119, Percent_Identity=34.453781512605, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI1790863, Length=118, Percent_Identity=30.5084745762712, Blast_Score=69, Evalue=9e-13,
Organism=Escherichia coli, GI87081977, Length=157, Percent_Identity=29.2993630573248, Blast_Score=69, Evalue=9e-13,
Organism=Escherichia coli, GI1789149, Length=113, Percent_Identity=35.3982300884956, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI87081974, Length=117, Percent_Identity=29.0598290598291, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1787056, Length=171, Percent_Identity=29.2397660818713, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI145693157, Length=103, Percent_Identity=34.9514563106796, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI48994928, Length=116, Percent_Identity=34.4827586206897, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1786911, Length=117, Percent_Identity=27.3504273504274, Blast_Score=62, Evalue=5e-11,
Organism=Escherichia coli, GI1787375, Length=118, Percent_Identity=29.6610169491525, Blast_Score=62, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 51945; Mature: 51813

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAKILVVDDVLSNVKLLEARLKAEYYTVIVAHDGEEAIDLVAKQQPDIILLDIMMPKMN
CCEEEEEHHHHHHHHHHHHHHHCCEEEEEEEEECCHHHHHHHHCCCCCEEEEEEECCCCC
GFQVCKELKSDPLTTHIPIIMVTALHDTHDRVQGINAGADDFLTKPINETALSARIKSLT
HHHHHHHHHCCCCCEECCEEEEEEECCCHHHHCCCCCCCHHHHCCCCCHHHHHHHHHHHH
RLKMIIDELRLRGETNAEIGGVAGNSIMDYSNQIFDANILVVDEDVFQAEQIYNVLKQRF
HHHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCCEEEEEEEEEECHHHHHHHHHHHHHHHH
RSIKILSDPIEALKVGIKDNYDLIISDMQFSKTDGLRLCSGFRSKVETRYTPILILSEDY
HHHHHHHCCHHHHHCCCCCCCEEEEECCEECCCCCHHHHHHHHHHHHCCCCEEEEEECCC
DKNNLVKALDVGANDYLTVPLDEGELIARVNSQVKRKRYQDALRMNLFNNAEMSIKDPLT
CCCCCEEEEECCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCHHH
NCYNRRYFDAHLRNIVKDSVEKDRRLSLMILDIDYFKMVNDNFGHSAGDELLKQIQRRIS
HHHCCCHHHHHHHHHHHHHHCCCCCEEEEEEECHHHHHHCCCCCCCHHHHHHHHHHHHHH
ENIRVTDLLARFGGEEFVVVMPDTDTSDAYTIAERIRKIIAKKPFILADKNTTHNVTVSI
CCCHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCEEEEEE
GIAEMQGSDLDNIKEFIVRADKYLYKAKNSGRNRVVTG
EEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
TAKILVVDDVLSNVKLLEARLKAEYYTVIVAHDGEEAIDLVAKQQPDIILLDIMMPKMN
CEEEEEHHHHHHHHHHHHHHHCCEEEEEEEEECCHHHHHHHHCCCCCEEEEEEECCCCC
GFQVCKELKSDPLTTHIPIIMVTALHDTHDRVQGINAGADDFLTKPINETALSARIKSLT
HHHHHHHHHCCCCCEECCEEEEEEECCCHHHHCCCCCCCHHHHCCCCCHHHHHHHHHHHH
RLKMIIDELRLRGETNAEIGGVAGNSIMDYSNQIFDANILVVDEDVFQAEQIYNVLKQRF
HHHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCCEEEEEEEEEECHHHHHHHHHHHHHHHH
RSIKILSDPIEALKVGIKDNYDLIISDMQFSKTDGLRLCSGFRSKVETRYTPILILSEDY
HHHHHHHCCHHHHHCCCCCCCEEEEECCEECCCCCHHHHHHHHHHHHCCCCEEEEEECCC
DKNNLVKALDVGANDYLTVPLDEGELIARVNSQVKRKRYQDALRMNLFNNAEMSIKDPLT
CCCCCEEEEECCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCHHH
NCYNRRYFDAHLRNIVKDSVEKDRRLSLMILDIDYFKMVNDNFGHSAGDELLKQIQRRIS
HHHCCCHHHHHHHHHHHHHHCCCCCEEEEEEECHHHHHHCCCCCCCHHHHHHHHHHHHHH
ENIRVTDLLARFGGEEFVVVMPDTDTSDAYTIAERIRKIIAKKPFILADKNTTHNVTVSI
CCCHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCEEEEEE
GIAEMQGSDLDNIKEFIVRADKYLYKAKNSGRNRVVTG
EEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA