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The map label for this gene is dacA [H]

Identifier: 225629999

GI number: 225629999

Start: 153894

End: 155000

Strand: Reverse

Name: dacA [H]

Synonym: WRi_001530

Alternate gene names: 225629999

Gene position: 155000-153894 (Counterclockwise)

Preceding gene: 225630000

Following gene: 225629998

Centisome position: 10.72

GC content: 34.24

Gene sequence:

>1107_bases
ATGTTAAGCAGATTGGTAATTTTGCTATTAGTTTTTATACTTCCTTTTTCTTCATACTCATACCAGTTTAGAACCAAAGC
AAAGCAAGCAGTAGTCTTAGATTTAGCTTCAGACTCGTTCATTTTTGACCATAATTCTGATGAAAAAATGGCCCCATCTT
CAATGAGCAAATTGATGACTTTATATATAGCCTTCGATTATCTAAAAGCTGGAATAATACACATGGAGGATAAATTTCGA
GTAAGTAGAAAAGCGTGGGAAAGAAAAGGCTCTTCTATGTTTTTGAAAGAAGGTCAATTTGTTACAGTGAGAGAATTACT
TGAAGGAATTACAATAGTTTCAGGTAATGATGCCTGCATAACACTAGCCGAGGGTATTGCAGGATCAGAAGAGAATTTTG
TGGCTGAAATGAATGAGGTTGCACAAAATTTGAATCTGAATGACAGTCATTTTGTCAACTCAAGCGGGTGGCCAGATGGA
GATCATTTTATGAGTGCAAAAGACTTGGTAATGCTGGCAAAAAGGATTTTCACTGATTTCCCTGAATATTATGATTTATT
TTCTGAACAATATTTGACATATAACGAAATTGTACAAAAAAATAAAAATCTTCTACTTTTTCACGACATTGGGGTTGATG
GCTTAAAAACCGGTTATACAAATGCTGGTGGTTACGGCATTGTAGCATCTGCAAAACGAAATGATAGGAGAATTTTTGCT
GTTGTAAATGGTTTAAACACTGAGAAAGAGCGAATAGAAGAAGCAAAAAGACTGATACAATATTCCTTAAATCATTTTAA
CACCAAGAAAATATTCGCTCAGGATAGCGTAGTTGAGGAAGTAAATGTTCTCTATGGAAAAGATAAAAAAGTGCCTATTA
CAGTTGCAAACGATGTTACCATAACTTACAACCGCAAATTACATGACCAAATTAAGGTGCGTATTGAATATAAAGATATG
ATACCTGCACCAATTAAAAAAGGTCAAGAAGTGGGCAAAGTTTTTGTAGAAATACCAGGTATTGAGCAACAAACTACACC
ACTTTATGCAGCAAATGACGTGCAGGAATTGAATTTCGTAGAAAAGTTTTTTAGGATGTTGTTTTAA

Upstream 100 bases:

>100_bases
TGGCGGAGTTTGTCAAGGAATTTTTATGCAAGAGATCTATTGAAATGCCCCTGTAAAATTATACTATTAATTAGTACACT
ATAAATTTAAAATTATAGAT

Downstream 100 bases:

>100_bases
GTTTGATATTAAAGCAATTTCGAAAGAGAGATTTATTGCACTTTAGTGTAGTTTTAACTAACAATTTCTGTTACAATACA
AGATTGAAGTGTTACGAAAA

Product: D-alanyl-D-alanine carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5; PBP-5 [H]

Number of amino acids: Translated: 368; Mature: 368

Protein sequence:

>368_residues
MLSRLVILLLVFILPFSSYSYQFRTKAKQAVVLDLASDSFIFDHNSDEKMAPSSMSKLMTLYIAFDYLKAGIIHMEDKFR
VSRKAWERKGSSMFLKEGQFVTVRELLEGITIVSGNDACITLAEGIAGSEENFVAEMNEVAQNLNLNDSHFVNSSGWPDG
DHFMSAKDLVMLAKRIFTDFPEYYDLFSEQYLTYNEIVQKNKNLLLFHDIGVDGLKTGYTNAGGYGIVASAKRNDRRIFA
VVNGLNTEKERIEEAKRLIQYSLNHFNTKKIFAQDSVVEEVNVLYGKDKKVPITVANDVTITYNRKLHDQIKVRIEYKDM
IPAPIKKGQEVGKVFVEIPGIEQQTTPLYAANDVQELNFVEKFFRMLF

Sequences:

>Translated_368_residues
MLSRLVILLLVFILPFSSYSYQFRTKAKQAVVLDLASDSFIFDHNSDEKMAPSSMSKLMTLYIAFDYLKAGIIHMEDKFR
VSRKAWERKGSSMFLKEGQFVTVRELLEGITIVSGNDACITLAEGIAGSEENFVAEMNEVAQNLNLNDSHFVNSSGWPDG
DHFMSAKDLVMLAKRIFTDFPEYYDLFSEQYLTYNEIVQKNKNLLLFHDIGVDGLKTGYTNAGGYGIVASAKRNDRRIFA
VVNGLNTEKERIEEAKRLIQYSLNHFNTKKIFAQDSVVEEVNVLYGKDKKVPITVANDVTITYNRKLHDQIKVRIEYKDM
IPAPIKKGQEVGKVFVEIPGIEQQTTPLYAANDVQELNFVEKFFRMLF
>Mature_368_residues
MLSRLVILLLVFILPFSSYSYQFRTKAKQAVVLDLASDSFIFDHNSDEKMAPSSMSKLMTLYIAFDYLKAGIIHMEDKFR
VSRKAWERKGSSMFLKEGQFVTVRELLEGITIVSGNDACITLAEGIAGSEENFVAEMNEVAQNLNLNDSHFVNSSGWPDG
DHFMSAKDLVMLAKRIFTDFPEYYDLFSEQYLTYNEIVQKNKNLLLFHDIGVDGLKTGYTNAGGYGIVASAKRNDRRIFA
VVNGLNTEKERIEEAKRLIQYSLNHFNTKKIFAQDSVVEEVNVLYGKDKKVPITVANDVTITYNRKLHDQIKVRIEYKDM
IPAPIKKGQEVGKVFVEIPGIEQQTTPLYAANDVQELNFVEKFFRMLF

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein. Note=N-terminal lies in the periplasmic space (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1787062, Length=346, Percent_Identity=34.1040462427746, Blast_Score=208, Evalue=6e-55,
Organism=Escherichia coli, GI87082022, Length=354, Percent_Identity=33.8983050847458, Blast_Score=193, Evalue=2e-50,
Organism=Escherichia coli, GI1786851, Length=353, Percent_Identity=31.4447592067989, Blast_Score=182, Evalue=2e-47,
Organism=Escherichia coli, GI87082054, Length=238, Percent_Identity=24.3697478991597, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 41994; Mature: 41994

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSRLVILLLVFILPFSSYSYQFRTKAKQAVVLDLASDSFIFDHNSDEKMAPSSMSKLMT
CHHHHHHHHHHHHHCCCCCCEEEHHCCCEEEEEEECCCCEEEECCCCCCCCHHHHHHHHH
LYIAFDYLKAGIIHMEDKFRVSRKAWERKGSSMFLKEGQFVTVRELLEGITIVSGNDACI
HHHHHHHHHCCEEEEHHHHHHHHHHHHCCCCEEEEECCCEEEHHHHHCCCEEEECCCCEE
TLAEGIAGSEENFVAEMNEVAQNLNLNDSHFVNSSGWPDGDHFMSAKDLVMLAKRIFTDF
EEECCCCCCCHHHHHHHHHHHHCCCCCCCCEECCCCCCCCHHHCCHHHHHHHHHHHHHCC
PEYYDLFSEQYLTYNEIVQKNKNLLLFHDIGVDGLKTGYTNAGGYGIVASAKRNDRRIFA
HHHHHHHHHHCCCHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCEEEEECCCCCCEEEE
VVNGLNTEKERIEEAKRLIQYSLNHFNTKKIFAQDSVVEEVNVLYGKDKKVPITVANDVT
EEECCCCHHHHHHHHHHHHHHHHHHCCCHHEEHHHHHHHHHHHHCCCCCCCEEEEECCEE
ITYNRKLHDQIKVRIEYKDMIPAPIKKGQEVGKVFVEIPGIEQQTTPLYAANDVQELNFV
EEECCEECEEEEEEEEECCCCCCCHHCCHHHCEEEEECCCCCCCCCCEEECCCHHHHHHH
EKFFRMLF
HHHHHHHC
>Mature Secondary Structure
MLSRLVILLLVFILPFSSYSYQFRTKAKQAVVLDLASDSFIFDHNSDEKMAPSSMSKLMT
CHHHHHHHHHHHHHCCCCCCEEEHHCCCEEEEEEECCCCEEEECCCCCCCCHHHHHHHHH
LYIAFDYLKAGIIHMEDKFRVSRKAWERKGSSMFLKEGQFVTVRELLEGITIVSGNDACI
HHHHHHHHHCCEEEEHHHHHHHHHHHHCCCCEEEEECCCEEEHHHHHCCCEEEECCCCEE
TLAEGIAGSEENFVAEMNEVAQNLNLNDSHFVNSSGWPDGDHFMSAKDLVMLAKRIFTDF
EEECCCCCCCHHHHHHHHHHHHCCCCCCCCEECCCCCCCCHHHCCHHHHHHHHHHHHHCC
PEYYDLFSEQYLTYNEIVQKNKNLLLFHDIGVDGLKTGYTNAGGYGIVASAKRNDRRIFA
HHHHHHHHHHCCCHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCEEEEECCCCCCEEEE
VVNGLNTEKERIEEAKRLIQYSLNHFNTKKIFAQDSVVEEVNVLYGKDKKVPITVANDVT
EEECCCCHHHHHHHHHHHHHHHHHHCCCHHEEHHHHHHHHHHHHCCCCCCCEEEEECCEE
ITYNRKLHDQIKVRIEYKDMIPAPIKKGQEVGKVFVEIPGIEQQTTPLYAANDVQELNFV
EEECCEECEEEEEEEEECCCCCCCHHCCHHHCEEEEECCCCCCCCCCEEECCCHHHHHHH
EKFFRMLF
HHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]