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The map label for this gene is ltrA [H]

Identifier: 225629983

GI number: 225629983

Start: 131458

End: 133005

Strand: Direct

Name: ltrA [H]

Synonym: WRi_001320

Alternate gene names: 225629983

Gene position: 131458-133005 (Clockwise)

Preceding gene: 225629982

Following gene: 225629984

Centisome position: 9.09

GC content: 37.47

Gene sequence:

>1548_bases
ATGATTACAAGTAAACCTGTAAGTGCATCTACCGAAAGCTTTGAAGCATGGAAGCAGTTGCCATGGAAGAAATGCCAGAA
AGTTATTGTGAGGCTACAAAGACGTATTGTTAAGGCTGTCCAAAAAGGAAGATGGGGTAAGGTAAAAGCTTTACAACATC
TTCTCACACGCTCTTTTAGCGGAAAAGCTTTGGCTGTTAAGAGAGTAACTGAAAACCAAGGAAAAAACACAGCGGGTGTA
GATCGTCAATTATGGTCAACTTGCAATGCTAAATTTCAAGGAATAAAGCAGTTAAAGCAAAGAGGATATAAACCTTCTCC
GCTAAAACGGATTTATATCAGTAAATCTAATGGCAAAAGAAGACCTCTTGGAATACCATCGATAAAAGATAGAGCCATGC
AAGCATTATATCTGTTTGCTCTGGAACCGATAGCTGAAACAATCAGTGATCGTCACTCCTATGGCTTTAGGCCTAAAAGA
TCCTGTGCAGATGCTACAGTAGCTTGTCATTTGTTACTGGCAAGTCGTAATCAACTACAATGGATACTTGAAGGTGATAT
CAAAGGATGCTTTGACAACATTAATCATGAATGGCTTATGAAGCATATTCCTATGGAGAAGAAAATTCTGCATAGTTGGT
TAAAAGCTGGCTTCCTAGAATCGAAAACTCTGTATCCCACAACTGCAGGTACCCCGCAAGGTGGTATAATTTCTCCAATA
CTAGCCAATTTAGCCTTAAACGGACTTGAAAAGTTATTGGAAAGTCGATTTGGTAAACTCGGCAGTAGAAGAAGAAACAA
AATCAGAAGTGGAGTGAACGTAATCAGATACGCAGACGACTTTATTATTTCAGGTTTCACACATGAAGTACTGGAAAATG
AAGTTAAGCCTTTAGTATCATCTTTTCTTCATGAGCGAGGTTTAATTCTTTCAGAAGAGAAGACAAAGATTACATCTATC
ACAACAGGGTTTGACTTTCTTGGTTGTAATGTACGTAGATATAATAAGAAGTTAATTATTAAACCTTCGAAAGAAAGTAT
TAAAAGACTTCTTAATAAAGCACGTACATTGATAAAAGCAAATATAGAGAACACTCAGGCTATAGTAATTAAGTCACTCA
ACTCTCTACTGAGAGGATGGGGAAATTACTATCACCATGTGTGTGCTAAAAAAGCGTTTAGAAAGATCGACAATGAAATT
TGGCATAGTTTATGGAAATGGGCAAAGAAAAGACATCCTCGTAAAGGATTACGTTGGATAAAGAATCGTTACTTCAAAGT
AATGGGTCAACGCCAATGGGTCTTTGCTGCACCCATATGCAAGAACAAACCAAAAGAGATACGGTATTTAAGACTGCTTA
AGCTGATTGATATACCTATCAGACGACATGTTAAAATCAGAGCGGATGCAAATCCCCTTGACTTAAAATGGAAAAAGTAT
TTTGATGAGAGAGTGAAACGAACAAGAATGTTAGCAAGCTCTTTCTCAAGAGAAGGTTCTCTACTGTTGGTGTCACCATT
AAGAATGATGTTTCCTGAGGAATCATGA

Upstream 100 bases:

>100_bases
TGGATAGAATTTGTAACATTAATTCGCGTGAAAACGAGCAGACTTCCGTTTGGTCCCTCGTGACAAGAAACTTTGTTAAC
CACTTAAGGTAGGAAAGCAG

Downstream 100 bases:

>100_bases
AGGACAAGCCGGTTCTAGAAAAGAAGAACTTAGATAAGGGGCTCAAGCGTAGTGTGGGGAAACCTGCATGCTGCGTTTCT
AAGGGAGGGGGTAGTAGTAA

Product: reverse transcriptase, putative

Products: NA

Alternate protein names: Reverse-transcriptase; RNA maturase; DNA endonuclease [H]

Number of amino acids: Translated: 515; Mature: 515

Protein sequence:

>515_residues
MITSKPVSASTESFEAWKQLPWKKCQKVIVRLQRRIVKAVQKGRWGKVKALQHLLTRSFSGKALAVKRVTENQGKNTAGV
DRQLWSTCNAKFQGIKQLKQRGYKPSPLKRIYISKSNGKRRPLGIPSIKDRAMQALYLFALEPIAETISDRHSYGFRPKR
SCADATVACHLLLASRNQLQWILEGDIKGCFDNINHEWLMKHIPMEKKILHSWLKAGFLESKTLYPTTAGTPQGGIISPI
LANLALNGLEKLLESRFGKLGSRRRNKIRSGVNVIRYADDFIISGFTHEVLENEVKPLVSSFLHERGLILSEEKTKITSI
TTGFDFLGCNVRRYNKKLIIKPSKESIKRLLNKARTLIKANIENTQAIVIKSLNSLLRGWGNYYHHVCAKKAFRKIDNEI
WHSLWKWAKKRHPRKGLRWIKNRYFKVMGQRQWVFAAPICKNKPKEIRYLRLLKLIDIPIRRHVKIRADANPLDLKWKKY
FDERVKRTRMLASSFSREGSLLLVSPLRMMFPEES

Sequences:

>Translated_515_residues
MITSKPVSASTESFEAWKQLPWKKCQKVIVRLQRRIVKAVQKGRWGKVKALQHLLTRSFSGKALAVKRVTENQGKNTAGV
DRQLWSTCNAKFQGIKQLKQRGYKPSPLKRIYISKSNGKRRPLGIPSIKDRAMQALYLFALEPIAETISDRHSYGFRPKR
SCADATVACHLLLASRNQLQWILEGDIKGCFDNINHEWLMKHIPMEKKILHSWLKAGFLESKTLYPTTAGTPQGGIISPI
LANLALNGLEKLLESRFGKLGSRRRNKIRSGVNVIRYADDFIISGFTHEVLENEVKPLVSSFLHERGLILSEEKTKITSI
TTGFDFLGCNVRRYNKKLIIKPSKESIKRLLNKARTLIKANIENTQAIVIKSLNSLLRGWGNYYHHVCAKKAFRKIDNEI
WHSLWKWAKKRHPRKGLRWIKNRYFKVMGQRQWVFAAPICKNKPKEIRYLRLLKLIDIPIRRHVKIRADANPLDLKWKKY
FDERVKRTRMLASSFSREGSLLLVSPLRMMFPEES
>Mature_515_residues
MITSKPVSASTESFEAWKQLPWKKCQKVIVRLQRRIVKAVQKGRWGKVKALQHLLTRSFSGKALAVKRVTENQGKNTAGV
DRQLWSTCNAKFQGIKQLKQRGYKPSPLKRIYISKSNGKRRPLGIPSIKDRAMQALYLFALEPIAETISDRHSYGFRPKR
SCADATVACHLLLASRNQLQWILEGDIKGCFDNINHEWLMKHIPMEKKILHSWLKAGFLESKTLYPTTAGTPQGGIISPI
LANLALNGLEKLLESRFGKLGSRRRNKIRSGVNVIRYADDFIISGFTHEVLENEVKPLVSSFLHERGLILSEEKTKITSI
TTGFDFLGCNVRRYNKKLIIKPSKESIKRLLNKARTLIKANIENTQAIVIKSLNSLLRGWGNYYHHVCAKKAFRKIDNEI
WHSLWKWAKKRHPRKGLRWIKNRYFKVMGQRQWVFAAPICKNKPKEIRYLRLLKLIDIPIRRHVKIRADANPLDLKWKKY
FDERVKRTRMLASSFSREGSLLLVSPLRMMFPEES

Specific function: Multifunctional protein that promotes group II intron splicing and mobility by acting both on RNA and DNA. It has three activities:reverse transcriptase (RT) for intron duplication, maturase to promote splicing, and DNA endonuclease for site- specific cle

COG id: COG3344

COG function: function code L; Retron-type reverse transcriptase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 reverse transcriptase domain [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6226521, Length=325, Percent_Identity=26.4615384615385, Blast_Score=111, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6226520, Length=317, Percent_Identity=27.4447949526814, Blast_Score=100, Evalue=5e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003615
- InterPro:   IPR002866
- InterPro:   IPR000477 [H]

Pfam domain/function: PF01348 Intron_maturas2; PF00078 RVT_1 [H]

EC number: =2.7.7.49 [H]

Molecular weight: Translated: 59583; Mature: 59583

Theoretical pI: Translated: 11.19; Mature: 11.19

Prosite motif: PS50878 RT_POL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITSKPVSASTESFEAWKQLPWKKCQKVIVRLQRRIVKAVQKGRWGKVKALQHLLTRSFS
CCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCC
GKALAVKRVTENQGKNTAGVDRQLWSTCNAKFQGIKQLKQRGYKPSPLKRIYISKSNGKR
CCEEEEEEHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCC
RPLGIPSIKDRAMQALYLFALEPIAETISDRHSYGFRPKRSCADATVACHLLLASRNQLQ
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCEE
WILEGDIKGCFDNINHEWLMKHIPMEKKILHSWLKAGFLESKTLYPTTAGTPQGGIISPI
EEEECCHHHHHHCCCHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHH
LANLALNGLEKLLESRFGKLGSRRRNKIRSGVNVIRYADDFIISGFTHEVLENEVKPLVS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHHH
SFLHERGLILSEEKTKITSITTGFDFLGCNVRRYNKKLIIKPSKESIKRLLNKARTLIKA
HHHHHCCCEECCCCCHHEEEECCCHHHCCCCEEECCEEEECCCHHHHHHHHHHHHHHHHH
NIENTQAIVIKSLNSLLRGWGNYYHHVCAKKAFRKIDNEIWHSLWKWAKKRHPRKGLRWI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
KNRYFKVMGQRQWVFAAPICKNKPKEIRYLRLLKLIDIPIRRHVKIRADANPLDLKWKKY
HHHHHHHHCCCCEEEECCHHCCCCHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHH
FDERVKRTRMLASSFSREGSLLLVSPLRMMFPEES
HHHHHHHHHHHHHHHCCCCCEEEECCHHHHCCCCC
>Mature Secondary Structure
MITSKPVSASTESFEAWKQLPWKKCQKVIVRLQRRIVKAVQKGRWGKVKALQHLLTRSFS
CCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCC
GKALAVKRVTENQGKNTAGVDRQLWSTCNAKFQGIKQLKQRGYKPSPLKRIYISKSNGKR
CCEEEEEEHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCC
RPLGIPSIKDRAMQALYLFALEPIAETISDRHSYGFRPKRSCADATVACHLLLASRNQLQ
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCEE
WILEGDIKGCFDNINHEWLMKHIPMEKKILHSWLKAGFLESKTLYPTTAGTPQGGIISPI
EEEECCHHHHHHCCCHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHH
LANLALNGLEKLLESRFGKLGSRRRNKIRSGVNVIRYADDFIISGFTHEVLENEVKPLVS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHHH
SFLHERGLILSEEKTKITSITTGFDFLGCNVRRYNKKLIIKPSKESIKRLLNKARTLIKA
HHHHHCCCEECCCCCHHEEEECCCHHHCCCCEEECCEEEECCCHHHHHHHHHHHHHHHHH
NIENTQAIVIKSLNSLLRGWGNYYHHVCAKKAFRKIDNEIWHSLWKWAKKRHPRKGLRWI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
KNRYFKVMGQRQWVFAAPICKNKPKEIRYLRLLKLIDIPIRRHVKIRADANPLDLKWKKY
HHHHHHHHCCCCEEEECCHHCCCCHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHH
FDERVKRTRMLASSFSREGSLLLVSPLRMMFPEES
HHHHHHHHHHHHHHHCCCCCEEEECCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8655550; 11157264; 10488339; 9353259; 10413481; 11731786 [H]