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The map label for this gene is ribE [H]

Identifier: 225629975

GI number: 225629975

Start: 124984

End: 125580

Strand: Direct

Name: ribE [H]

Synonym: WRi_001220

Alternate gene names: 225629975

Gene position: 124984-125580 (Clockwise)

Preceding gene: 225629974

Following gene: 225629976

Centisome position: 8.64

GC content: 32.66

Gene sequence:

>597_bases
ATGTTTAAAGGAATTATCACGGATATCGGGACTATAACTGATACTACCACTCACTCCAACTCTGATCAAATTTTCCATAT
CAAGACACAAAATTTATCCTCTATAAATAAAGGAGATTCAATAGCTTGCTCTGGTGTGTGTTTAACTGTTGTTGATATAA
TGAGCGATACATTTACAGTCCAAGCATCTCAAGAAACTATGAAGGTTGCTAACTTAAATACGTGGAGAATAGGAAAAAAA
ATAAATCTCGAACAAGCAATGAGATTAAGCGATAAGATCGATGGCCACCTGGTTCAGGGTCATGTTGATGGGATAGTAAA
ATTTTTAACAATTAATCAAAATTTAGATTCTCATGAAATCAAACTATCGTGCCCACAAGAATTAATTAAATTTGTTGCAA
AAAAAAGCTCCGTTACACTAGATGGGGTTTCCCTCACAGTGAATTCAGTTGTTAATCAGGAATTTACAGTAAATATCACT
CCCTATACATGGGAAAATACAACTTTTCAGTACAACAAAGTAAATGATTATTTGAATTTAGAAATTGACATGATCGCTAG
GTATTTAGATCAATTGATGCAGCATAAATACAATTAA

Upstream 100 bases:

>100_bases
TTTTTAAATTGTCGGTAAACCAGAATTACTTTAACTATAACAAAATAGAAATAAGTTGTTGTAAAAAATTTTTCATGGTA
AAATTATTTATTTTCACCAT

Downstream 100 bases:

>100_bases
TTTTACAACCTTGTTATTTACAACTATTATATCTTGAAAACCATATAGATATAAAACATGCTTTTCGATAGCCTAATTAT
CTTCATTGTTGTTCTATGCG

Product: riboflavin synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 198; Mature: 198

Protein sequence:

>198_residues
MFKGIITDIGTITDTTTHSNSDQIFHIKTQNLSSINKGDSIACSGVCLTVVDIMSDTFTVQASQETMKVANLNTWRIGKK
INLEQAMRLSDKIDGHLVQGHVDGIVKFLTINQNLDSHEIKLSCPQELIKFVAKKSSVTLDGVSLTVNSVVNQEFTVNIT
PYTWENTTFQYNKVNDYLNLEIDMIARYLDQLMQHKYN

Sequences:

>Translated_198_residues
MFKGIITDIGTITDTTTHSNSDQIFHIKTQNLSSINKGDSIACSGVCLTVVDIMSDTFTVQASQETMKVANLNTWRIGKK
INLEQAMRLSDKIDGHLVQGHVDGIVKFLTINQNLDSHEIKLSCPQELIKFVAKKSSVTLDGVSLTVNSVVNQEFTVNIT
PYTWENTTFQYNKVNDYLNLEIDMIARYLDQLMQHKYN
>Mature_198_residues
MFKGIITDIGTITDTTTHSNSDQIFHIKTQNLSSINKGDSIACSGVCLTVVDIMSDTFTVQASQETMKVANLNTWRIGKK
INLEQAMRLSDKIDGHLVQGHVDGIVKFLTINQNLDSHEIKLSCPQELIKFVAKKSSVTLDGVSLTVNSVVNQEFTVNIT
PYTWENTTFQYNKVNDYLNLEIDMIARYLDQLMQHKYN

Specific function: Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The alpha subunit catalyze

COG id: COG0307

COG function: function code H; Riboflavin synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 lumazine-binding repeats [H]

Homologues:

Organism=Escherichia coli, GI1787952, Length=185, Percent_Identity=29.7297297297297, Blast_Score=98, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6319733, Length=203, Percent_Identity=31.5270935960591, Blast_Score=104, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001783
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00677 Lum_binding [H]

EC number: =2.5.1.9 [H]

Molecular weight: Translated: 22307; Mature: 22307

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: PS00430 TONB_DEPENDENT_REC_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKGIITDIGTITDTTTHSNSDQIFHIKTQNLSSINKGDSIACSGVCLTVVDIMSDTFTV
CCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCEEECHHHHHHHHHHCCCEEE
QASQETMKVANLNTWRIGKKINLEQAMRLSDKIDGHLVQGHVDGIVKFLTINQNLDSHEI
EECHHHEEEECCCEEECCCEECHHHHHHHHHCCCCEEEEEHHCCEEEEEEECCCCCCCEE
KLSCPQELIKFVAKKSSVTLDGVSLTVNSVVNQEFTVNITPYTWENTTFQYNKVNDYLNL
EEECHHHHHHHHHCCCCEEECCCEEEEEHHCCCEEEEEECCEEECCCEEEEECCCCEEEE
EIDMIARYLDQLMQHKYN
EHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MFKGIITDIGTITDTTTHSNSDQIFHIKTQNLSSINKGDSIACSGVCLTVVDIMSDTFTV
CCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCEEECHHHHHHHHHHCCCEEE
QASQETMKVANLNTWRIGKKINLEQAMRLSDKIDGHLVQGHVDGIVKFLTINQNLDSHEI
EECHHHEEEECCCEEECCCEECHHHHHHHHHCCCCEEEEEHHCCEEEEEEECCCCCCCEE
KLSCPQELIKFVAKKSSVTLDGVSLTVNSVVNQEFTVNITPYTWENTTFQYNKVNDYLNL
EEECHHHHHHHHHCCCCEEECCCEEEEEHHCCCEEEEEECCEEECCCEEEEECCCCEEEE
EIDMIARYLDQLMQHKYN
EHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]