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The map label for this gene is ruvC [H]

Identifier: 225629965

GI number: 225629965

Start: 114599

End: 115087

Strand: Reverse

Name: ruvC [H]

Synonym: WRi_001110

Alternate gene names: 225629965

Gene position: 115087-114599 (Counterclockwise)

Preceding gene: 225629966

Following gene: 225629962

Centisome position: 7.96

GC content: 34.15

Gene sequence:

>489_bases
GTGGTTAAAATAATAGGTCTAGATCCAGGAATAAGCAAGACTGGGTGGGCCATTATCAGCCTGAACGAAAAAAATAATAT
TGAATTTTTGGGCGGCGGTACCATATCAACTGATGGTAAATTGGGTACAGGTGAACGCCTACATATAATTTTTGAACAAT
TAAAGAAAGTAATTTCCCTATACTCTCCGAATGAAGCTGCGGTGGAGAAAATTTTTGTTAATAAAAATCCTAAATCTTCA
CTAACTTTAGGATATGCAAGAGGGGTTGTAATTTTAGCGTTAAAAATAACAAAGCTAACTATGAATGAATATGATGCAAA
CTATGTAAAAAAAAGCATTACCGGAAATGGCCATGCTGATAAGGATCAGATTATATTTATGGTGAAGCAGATAGTTAAAA
ATTTGAATATAAAATGTCACCATGCTGCTGATGCTCTTGCTGTAGCAATTTGTCATGCTTATACGAAAGGTTCTTGTTTT
GTTGAGTAA

Upstream 100 bases:

>100_bases
GGACCATTTGTGCTTTGAGTTTGCCTCCTGGTATTGGCACTTTGTAGATGTAGTCTGGATATTTTTATTTTTGTTTGTCT
ATTGGTTAAGCGTTTATTAA

Downstream 100 bases:

>100_bases
CTTTTATATGGGATTCAGTTTGAAGATTTCCAGATCTGGAAAAACTATTTGATTGTATTTATCTTGTCTAACGTTCGTTG
CAAAATAATACATGCGGAGA

Product: crossover junction endodeoxyribonuclease RuvC

Products: NA

Alternate protein names: Holliday junction nuclease ruvC; Holliday junction resolvase ruvC [H]

Number of amino acids: Translated: 162; Mature: 162

Protein sequence:

>162_residues
MVKIIGLDPGISKTGWAIISLNEKNNIEFLGGGTISTDGKLGTGERLHIIFEQLKKVISLYSPNEAAVEKIFVNKNPKSS
LTLGYARGVVILALKITKLTMNEYDANYVKKSITGNGHADKDQIIFMVKQIVKNLNIKCHHAADALAVAICHAYTKGSCF
VE

Sequences:

>Translated_162_residues
MVKIIGLDPGISKTGWAIISLNEKNNIEFLGGGTISTDGKLGTGERLHIIFEQLKKVISLYSPNEAAVEKIFVNKNPKSS
LTLGYARGVVILALKITKLTMNEYDANYVKKSITGNGHADKDQIIFMVKQIVKNLNIKCHHAADALAVAICHAYTKGSCF
VE
>Mature_162_residues
MVKIIGLDPGISKTGWAIISLNEKNNIEFLGGGTISTDGKLGTGERLHIIFEQLKKVISLYSPNEAAVEKIFVNKNPKSS
LTLGYARGVVILALKITKLTMNEYDANYVKKSITGNGHADKDQIIFMVKQIVKNLNIKCHHAADALAVAICHAYTKGSCF
VE

Specific function: Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leav

COG id: COG0817

COG function: function code L; Holliday junction resolvasome, endonuclease subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvC family [H]

Homologues:

Organism=Escherichia coli, GI1788170, Length=133, Percent_Identity=36.0902255639098, Blast_Score=87, Evalue=6e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012337
- InterPro:   IPR002176 [H]

Pfam domain/function: PF02075 RuvC [H]

EC number: =3.1.22.4 [H]

Molecular weight: Translated: 17660; Mature: 17660

Theoretical pI: Translated: 9.61; Mature: 9.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKIIGLDPGISKTGWAIISLNEKNNIEFLGGGTISTDGKLGTGERLHIIFEQLKKVISL
CEEEEECCCCCCCCCEEEEEECCCCCEEEEECCEEECCCCCCCCCHHHHHHHHHHHHHHH
YSPNEAAVEKIFVNKNPKSSLTLGYARGVVILALKITKLTMNEYDANYVKKSITGNGHAD
CCCCHHHHHEEEECCCCCCCEEEEHHCCEEEEEEEEEEEECCCCCHHHHHHHCCCCCCCC
KDQIIFMVKQIVKNLNIKCHHAADALAVAICHAYTKGSCFVE
HHHHHHHHHHHHHHCCEEEECHHHHHHHHHHHHHCCCCEEEC
>Mature Secondary Structure
MVKIIGLDPGISKTGWAIISLNEKNNIEFLGGGTISTDGKLGTGERLHIIFEQLKKVISL
CEEEEECCCCCCCCCEEEEEECCCCCEEEEECCEEECCCCCCCCCHHHHHHHHHHHHHHH
YSPNEAAVEKIFVNKNPKSSLTLGYARGVVILALKITKLTMNEYDANYVKKSITGNGHAD
CCCCHHHHHEEEECCCCCCCEEEEHHCCEEEEEEEEEEEECCCCCHHHHHHHCCCCCCCC
KDQIIFMVKQIVKNLNIKCHHAADALAVAICHAYTKGSCFVE
HHHHHHHHHHHHHHCCEEEECHHHHHHHHHHHHHCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA