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The map label for this gene is tuf1 [H]

Identifier: 225629886

GI number: 225629886

Start: 16396

End: 17568

Strand: Direct

Name: tuf1 [H]

Synonym: WRi_000150

Alternate gene names: 225629886

Gene position: 16396-17568 (Clockwise)

Preceding gene: 225629885

Following gene: 225629887

Centisome position: 1.13

GC content: 43.05

Gene sequence:

>1173_bases
ATGACAGCAGTAGTGGAAGCATTTGGAAAGCCGCATGTAAATGTGGGAACAATAGGACATGTGGATCATGGGAAGACAAC
GTTAACAGCGGCAATAACAAAGCATTATGGAAATTTTGTAGCGTACGATCAGATAGATAAAGCGCCAGAAGAAAGGAAGA
GGGGGATAACGATAGCAACAGCGCATGTTGAGTATCAGACAGAGAAAAGGCATTATGCACACGTTGATTGTCCTGGGCAC
GCTGACTATGTAAAGAACATGATAGTAGGTGCAGCACAGATGGATGCGGCGATATTGGTAGTGTCAGGGGTTGATGGGCC
AATGCCACAAACGAGAGAGCATATATTGCTGGCGAAGCAGGTAGGTGTTGGATATATCGTGGTGTATATAAATAAAGCTG
ATGTTGCTGATGCTGATATGATAGATTTAGTAGAAATGGAAGTGAGGGAATTACTGAGTAAATACGGATTTCCAGGGGAT
GAAGTGCCTGTGGTGGTTGGTTCAGCGCTCAAGGCGCTGGAGGATGACAGCAGCGAATATGGAAAGAAATCAATAGACAA
ATTGATGGAAAAGTTAGATGAATATGTAGCGGTTCCACCAAGGCCTGTGGATTTACCATTTTTATTGCCAATCGAAGATG
TGTTTTCGATATCGGGGCGAGGAACGGTGGTAACAGGAAGGATAGAGAAGGGAGAGATAAAGACGGGAGAAGAGATAGAG
ATAATAGGTCTGAAGGGAACGCAAAAGACGATATGCACAGGTGTAGAAATGTTTAAGAAGTTGCTGGATAAGGGAAGTGC
AGGACTCAATGTAGGAATATTGTTAAGAGGAACAAAAAGAGAAGAAGTAGAGAGAGGGCAAGTATTGGCAAAACCGGGGA
CGATAACGCCGCATAGAAAGTTTAAGGCGGAGGTTTATATATTAAAGAAAGAGGAAGGAGGAAGGCATACACCATTTTTT
GCGAATTACCAGCCACAGTTTTATTTAAGGACAACGGATGTAACTGGGAGCATAAAATTGCTAGATGGGAAGGAGATGGT
AATGCCAGGAGATAATGTGAGTGTAGAAGTAGAATTGCAAGTACCGATAGCAATGGATAAGGGATTGCGTTTTGCGATAA
GAGAAGGCGGTAGAACTGTTGGTTCTGGTGTTGTTTCTGAAATTTTGGAGTGA

Upstream 100 bases:

>100_bases
AGGGAAGGGCGCAGTATAGTATGCATTTTTCTTGTTATGAGCAAGTGCCACAATATGTGGTTGATGAGTTAAAGTTAGAG
TATAATTAAGGGTAATAATT

Downstream 100 bases:

>100_bases
GAGTTTGAGTTATAGGAGTGTAGCTCAATTGGTAGAGCGCTGGTCTCCAAAACCAGAGGTTGTAGGTTCAATTCCTATCG
CTCCTGCATTAATGTTGTGG

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu 1 [H]

Number of amino acids: Translated: 390; Mature: 389

Protein sequence:

>390_residues
MTAVVEAFGKPHVNVGTIGHVDHGKTTLTAAITKHYGNFVAYDQIDKAPEERKRGITIATAHVEYQTEKRHYAHVDCPGH
ADYVKNMIVGAAQMDAAILVVSGVDGPMPQTREHILLAKQVGVGYIVVYINKADVADADMIDLVEMEVRELLSKYGFPGD
EVPVVVGSALKALEDDSSEYGKKSIDKLMEKLDEYVAVPPRPVDLPFLLPIEDVFSISGRGTVVTGRIEKGEIKTGEEIE
IIGLKGTQKTICTGVEMFKKLLDKGSAGLNVGILLRGTKREEVERGQVLAKPGTITPHRKFKAEVYILKKEEGGRHTPFF
ANYQPQFYLRTTDVTGSIKLLDGKEMVMPGDNVSVEVELQVPIAMDKGLRFAIREGGRTVGSGVVSEILE

Sequences:

>Translated_390_residues
MTAVVEAFGKPHVNVGTIGHVDHGKTTLTAAITKHYGNFVAYDQIDKAPEERKRGITIATAHVEYQTEKRHYAHVDCPGH
ADYVKNMIVGAAQMDAAILVVSGVDGPMPQTREHILLAKQVGVGYIVVYINKADVADADMIDLVEMEVRELLSKYGFPGD
EVPVVVGSALKALEDDSSEYGKKSIDKLMEKLDEYVAVPPRPVDLPFLLPIEDVFSISGRGTVVTGRIEKGEIKTGEEIE
IIGLKGTQKTICTGVEMFKKLLDKGSAGLNVGILLRGTKREEVERGQVLAKPGTITPHRKFKAEVYILKKEEGGRHTPFF
ANYQPQFYLRTTDVTGSIKLLDGKEMVMPGDNVSVEVELQVPIAMDKGLRFAIREGGRTVGSGVVSEILE
>Mature_389_residues
TAVVEAFGKPHVNVGTIGHVDHGKTTLTAAITKHYGNFVAYDQIDKAPEERKRGITIATAHVEYQTEKRHYAHVDCPGHA
DYVKNMIVGAAQMDAAILVVSGVDGPMPQTREHILLAKQVGVGYIVVYINKADVADADMIDLVEMEVRELLSKYGFPGDE
VPVVVGSALKALEDDSSEYGKKSIDKLMEKLDEYVAVPPRPVDLPFLLPIEDVFSISGRGTVVTGRIEKGEIKTGEEIEI
IGLKGTQKTICTGVEMFKKLLDKGSAGLNVGILLRGTKREEVERGQVLAKPGTITPHRKFKAEVYILKKEEGGRHTPFFA
NYQPQFYLRTTDVTGSIKLLDGKEMVMPGDNVSVEVELQVPIAMDKGLRFAIREGGRTVGSGVVSEILE

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=386, Percent_Identity=56.2176165803109, Blast_Score=456, Evalue=1e-128,
Organism=Homo sapiens, GI223555963, Length=433, Percent_Identity=30.9468822170901, Blast_Score=156, Evalue=3e-38,
Organism=Homo sapiens, GI5729864, Length=433, Percent_Identity=30.9468822170901, Blast_Score=156, Evalue=3e-38,
Organism=Homo sapiens, GI4503471, Length=440, Percent_Identity=29.3181818181818, Blast_Score=147, Evalue=1e-35,
Organism=Homo sapiens, GI4503475, Length=440, Percent_Identity=29.0909090909091, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI194018520, Length=338, Percent_Identity=31.0650887573964, Blast_Score=128, Evalue=7e-30,
Organism=Homo sapiens, GI194097354, Length=338, Percent_Identity=31.0650887573964, Blast_Score=128, Evalue=8e-30,
Organism=Homo sapiens, GI194018522, Length=437, Percent_Identity=28.1464530892449, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI46094014, Length=377, Percent_Identity=28.6472148541114, Blast_Score=123, Evalue=3e-28,
Organism=Homo sapiens, GI54607086, Length=316, Percent_Identity=28.4810126582279, Blast_Score=116, Evalue=4e-26,
Organism=Homo sapiens, GI4503507, Length=267, Percent_Identity=27.7153558052434, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI53729339, Length=243, Percent_Identity=32.5102880658436, Blast_Score=65, Evalue=9e-11,
Organism=Homo sapiens, GI53729337, Length=243, Percent_Identity=32.5102880658436, Blast_Score=65, Evalue=9e-11,
Organism=Escherichia coli, GI1790412, Length=386, Percent_Identity=70.2072538860104, Blast_Score=536, Evalue=1e-153,
Organism=Escherichia coli, GI1789737, Length=386, Percent_Identity=70.2072538860104, Blast_Score=535, Evalue=1e-153,
Organism=Escherichia coli, GI2367247, Length=366, Percent_Identity=29.5081967213115, Blast_Score=139, Evalue=4e-34,
Organism=Escherichia coli, GI48994988, Length=248, Percent_Identity=31.0483870967742, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1789108, Length=249, Percent_Identity=28.5140562248996, Blast_Score=84, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17556456, Length=396, Percent_Identity=49.2424242424242, Blast_Score=371, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI25141371, Length=386, Percent_Identity=43.2642487046632, Blast_Score=323, Evalue=8e-89,
Organism=Caenorhabditis elegans, GI17552884, Length=440, Percent_Identity=29.7727272727273, Blast_Score=151, Evalue=4e-37,
Organism=Caenorhabditis elegans, GI17569207, Length=440, Percent_Identity=29.7727272727273, Blast_Score=151, Evalue=4e-37,
Organism=Caenorhabditis elegans, GI115532067, Length=435, Percent_Identity=28.2758620689655, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI115532065, Length=435, Percent_Identity=28.2758620689655, Blast_Score=132, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI32566303, Length=427, Percent_Identity=28.3372365339578, Blast_Score=125, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI32566629, Length=437, Percent_Identity=26.3157894736842, Blast_Score=115, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI17506081, Length=267, Percent_Identity=29.5880149812734, Blast_Score=112, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI32566301, Length=145, Percent_Identity=37.2413793103448, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17509919, Length=279, Percent_Identity=28.673835125448, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17557151, Length=230, Percent_Identity=28.2608695652174, Blast_Score=79, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6324761, Length=402, Percent_Identity=59.7014925373134, Blast_Score=489, Evalue=1e-139,
Organism=Saccharomyces cerevisiae, GI6325337, Length=438, Percent_Identity=30.1369863013699, Blast_Score=154, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6319594, Length=438, Percent_Identity=30.1369863013699, Blast_Score=154, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6320377, Length=339, Percent_Identity=32.1533923303835, Blast_Score=100, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6322937, Length=468, Percent_Identity=24.5726495726496, Blast_Score=97, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6320863, Length=277, Percent_Identity=28.5198555956679, Blast_Score=83, Evalue=7e-17,
Organism=Saccharomyces cerevisiae, GI6322359, Length=175, Percent_Identity=29.7142857142857, Blast_Score=68, Evalue=3e-12,
Organism=Drosophila melanogaster, GI281363316, Length=385, Percent_Identity=55.8441558441558, Blast_Score=452, Evalue=1e-127,
Organism=Drosophila melanogaster, GI17864358, Length=385, Percent_Identity=55.8441558441558, Blast_Score=452, Evalue=1e-127,
Organism=Drosophila melanogaster, GI19921738, Length=384, Percent_Identity=49.7395833333333, Blast_Score=397, Evalue=1e-111,
Organism=Drosophila melanogaster, GI45553807, Length=441, Percent_Identity=27.891156462585, Blast_Score=141, Evalue=9e-34,
Organism=Drosophila melanogaster, GI45553816, Length=441, Percent_Identity=27.891156462585, Blast_Score=141, Evalue=9e-34,
Organism=Drosophila melanogaster, GI24651721, Length=441, Percent_Identity=27.891156462585, Blast_Score=141, Evalue=9e-34,
Organism=Drosophila melanogaster, GI17864154, Length=441, Percent_Identity=27.891156462585, Blast_Score=141, Evalue=9e-34,
Organism=Drosophila melanogaster, GI24652838, Length=441, Percent_Identity=27.6643990929705, Blast_Score=140, Evalue=1e-33,
Organism=Drosophila melanogaster, GI17137572, Length=441, Percent_Identity=27.6643990929705, Blast_Score=140, Evalue=1e-33,
Organism=Drosophila melanogaster, GI19922690, Length=300, Percent_Identity=31, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI17137380, Length=345, Percent_Identity=31.304347826087, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI45550900, Length=435, Percent_Identity=26.2068965517241, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24647046, Length=277, Percent_Identity=27.7978339350181, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24647048, Length=277, Percent_Identity=27.7978339350181, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI78706572, Length=243, Percent_Identity=27.9835390946502, Blast_Score=66, Evalue=4e-11,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 42627; Mature: 42496

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAVVEAFGKPHVNVGTIGHVDHGKTTLTAAITKHYGNFVAYDQIDKAPEERKRGITIAT
CCCCHHHCCCCCCCCCEEECCCCCCEEEHHHHHHHCCCEEEEHHHHCCHHHHHCCEEEEE
AHVEYQTEKRHYAHVDCPGHADYVKNMIVGAAQMDAAILVVSGVDGPMPQTREHILLAKQ
EEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCHHHEEEEEE
VGVGYIVVYINKADVADADMIDLVEMEVRELLSKYGFPGDEVPVVVGSALKALEDDSSEY
CCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHCCCCHHH
GKKSIDKLMEKLDEYVAVPPRPVDLPFLLPIEDVFSISGRGTVVTGRIEKGEIKTGEEIE
HHHHHHHHHHHHHHHCCCCCCCCCCEEECCHHHHHCCCCCCEEEEEEEECCCCCCCCEEE
IIGLKGTQKTICTGVEMFKKLLDKGSAGLNVGILLRGTKREEVERGQVLAKPGTITPHRK
EEEECCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHCCCEEECCCCCCCCCC
FKAEVYILKKEEGGRHTPFFANYQPQFYLRTTDVTGSIKLLDGKEMVMPGDNVSVEVELQ
EEEEEEEEEECCCCCCCCEEECCCCEEEEEEECCCCEEEEECCCEEEECCCCEEEEEEEE
VPIAMDKGLRFAIREGGRTVGSGVVSEILE
EEEEECCCCEEEECCCCCCHHHHHHHHHCC
>Mature Secondary Structure 
TAVVEAFGKPHVNVGTIGHVDHGKTTLTAAITKHYGNFVAYDQIDKAPEERKRGITIAT
CCCHHHCCCCCCCCCEEECCCCCCEEEHHHHHHHCCCEEEEHHHHCCHHHHHCCEEEEE
AHVEYQTEKRHYAHVDCPGHADYVKNMIVGAAQMDAAILVVSGVDGPMPQTREHILLAKQ
EEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCHHHEEEEEE
VGVGYIVVYINKADVADADMIDLVEMEVRELLSKYGFPGDEVPVVVGSALKALEDDSSEY
CCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHCCCCHHH
GKKSIDKLMEKLDEYVAVPPRPVDLPFLLPIEDVFSISGRGTVVTGRIEKGEIKTGEEIE
HHHHHHHHHHHHHHHCCCCCCCCCCEEECCHHHHHCCCCCCEEEEEEEECCCCCCCCEEE
IIGLKGTQKTICTGVEMFKKLLDKGSAGLNVGILLRGTKREEVERGQVLAKPGTITPHRK
EEEECCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHCCCEEECCCCCCCCCC
FKAEVYILKKEEGGRHTPFFANYQPQFYLRTTDVTGSIKLLDGKEMVMPGDNVSVEVELQ
EEEEEEEEEECCCCCCCCEEECCCCEEEEEEECCCCEEEEECCCEEEECCCCEEEEEEEE
VPIAMDKGLRFAIREGGRTVGSGVVSEILE
EEEEECCCCEEEECCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA