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The map label for this gene is pleD [H]

Identifier: 222527011

GI number: 222527011

Start: 4721753

End: 4722865

Strand: Reverse

Name: pleD [H]

Synonym: Chy400_3790

Alternate gene names: 222527011

Gene position: 4722865-4721753 (Counterclockwise)

Preceding gene: 222527013

Following gene: 222527008

Centisome position: 89.64

GC content: 55.26

Gene sequence:

>1113_bases
ATGACTGAGGAACAGCCAGACAATACCGATGATCAGATCGTTCTACCCCCAATCCCGCCGAATGAAGAAGAACGCCTGCG
TGAACTGGAGAGCTTTCAGATCCTTGATACACTCCCTGAGCAGATCTTCGAGGACATTGTACGCATCGCGGCGTACATCT
GCCAGACCCCGATTGCCCTGGTGAACCTGGTTGACCGCCATCGCCAGTGGTCGATGGCGCGGTTGGGGTTCAGCGATGGC
GAAGTCGAGCGTACTCTCACGTTTTGTGCATTCACAATCAATGATCCTGATCACGTGATGGTAGTGCCAGATGCAACCCG
TGATCCGCGCTTTGCCAGCAATCCCTATGTGCGCCGCGAACCGTACATTCGCTTTTACGCCGGTGCGCCACTCGTAACTT
CACACGGGTATGCCCTTGGCACGGTTTGTGTTATCGATCTTCAGCCGCGTCAGCTCTCGCCGGAACAGCTTGAGATGCTG
CGCTTACTGTCACGGCAGGTTGTGACTCAGCTTGAAATCCGGCGTGATCTGTTGCGCCTCGAACAGCGTCTGCTACAGCG
CGAACAGGAGTATCTATCGCTGAATGCGTACCTTCAGAGTCTGGAAGACAAACTGGCAGCCGCACAACAGCAATCGCTCA
CCGATCCGTTGACCGGTGTGTTCAACCGGCGTGGCTTTGCGTTGCGCCTCAGCGAAGAGATTGAGCGGGCGCGGCGTTAC
CAGGTAACTCTGGCGCTGCTGTTGATCGATGTCGATCATTTCAAGCAATACAACGATACCTTTGGGCATCTTGCCGGCGA
TGAAGTGCTCCGCATGATTGCCCAACTTCTCAGTGAAGGTTGCTGCAAACACGACATTGTCGCCCGGTATGGTGGTGAAG
AATTTGCTGTTATCCTACCGGCAACTGCCGGCGACGGCGCTCAGGTCTTGGGCGAGAGGTTACGCCGGTTGGTGCAGATG
GCGGCCTGGCCGCTGCATCCATTGACAATCAGTGTGGGTGGTGCAGTCTTACAACCTGAGATGACCGATGAGCATGACCT
GATTGCACGTGCTGACAGTGCGTTATACCAGGCAAAAGCACAGGGTCGTAATACGTGTGTCATCATTGCCTGA

Upstream 100 bases:

>100_bases
TCCTTCAATTATTGTGACAGAATTGGATTTTCGATCACATTTGTGTTTTCTTATACAATATCTACAGGAGACAGTCTATC
CCCCGCTGAGGTTGTAACTG

Downstream 100 bases:

>100_bases
CCCGCAGGCTATGGTTGCGCTAACCCATCGAAAAAGGTCGTAAGGATCAGGTCTGCAATTACGTGACCCTGCTCGCCAGC
CGGTGCCTGATCGGTGTGAA

Product: diguanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 370; Mature: 369

Protein sequence:

>370_residues
MTEEQPDNTDDQIVLPPIPPNEEERLRELESFQILDTLPEQIFEDIVRIAAYICQTPIALVNLVDRHRQWSMARLGFSDG
EVERTLTFCAFTINDPDHVMVVPDATRDPRFASNPYVRREPYIRFYAGAPLVTSHGYALGTVCVIDLQPRQLSPEQLEML
RLLSRQVVTQLEIRRDLLRLEQRLLQREQEYLSLNAYLQSLEDKLAAAQQQSLTDPLTGVFNRRGFALRLSEEIERARRY
QVTLALLLIDVDHFKQYNDTFGHLAGDEVLRMIAQLLSEGCCKHDIVARYGGEEFAVILPATAGDGAQVLGERLRRLVQM
AAWPLHPLTISVGGAVLQPEMTDEHDLIARADSALYQAKAQGRNTCVIIA

Sequences:

>Translated_370_residues
MTEEQPDNTDDQIVLPPIPPNEEERLRELESFQILDTLPEQIFEDIVRIAAYICQTPIALVNLVDRHRQWSMARLGFSDG
EVERTLTFCAFTINDPDHVMVVPDATRDPRFASNPYVRREPYIRFYAGAPLVTSHGYALGTVCVIDLQPRQLSPEQLEML
RLLSRQVVTQLEIRRDLLRLEQRLLQREQEYLSLNAYLQSLEDKLAAAQQQSLTDPLTGVFNRRGFALRLSEEIERARRY
QVTLALLLIDVDHFKQYNDTFGHLAGDEVLRMIAQLLSEGCCKHDIVARYGGEEFAVILPATAGDGAQVLGERLRRLVQM
AAWPLHPLTISVGGAVLQPEMTDEHDLIARADSALYQAKAQGRNTCVIIA
>Mature_369_residues
TEEQPDNTDDQIVLPPIPPNEEERLRELESFQILDTLPEQIFEDIVRIAAYICQTPIALVNLVDRHRQWSMARLGFSDGE
VERTLTFCAFTINDPDHVMVVPDATRDPRFASNPYVRREPYIRFYAGAPLVTSHGYALGTVCVIDLQPRQLSPEQLEMLR
LLSRQVVTQLEIRRDLLRLEQRLLQREQEYLSLNAYLQSLEDKLAAAQQQSLTDPLTGVFNRRGFALRLSEEIERARRYQ
VTLALLLIDVDHFKQYNDTFGHLAGDEVLRMIAQLLSEGCCKHDIVARYGGEEFAVILPATAGDGAQVLGERLRRLVQMA
AWPLHPLTISVGGAVLQPEMTDEHDLIARADSALYQAKAQGRNTCVIIA

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1786584, Length=165, Percent_Identity=38.7878787878788, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1787262, Length=161, Percent_Identity=36.0248447204969, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI145693134, Length=155, Percent_Identity=38.0645161290323, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI87081881, Length=173, Percent_Identity=39.8843930635838, Blast_Score=95, Evalue=9e-21,
Organism=Escherichia coli, GI1787816, Length=164, Percent_Identity=37.1951219512195, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI87082007, Length=165, Percent_Identity=35.1515151515151, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI87081977, Length=247, Percent_Identity=26.7206477732794, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1787802, Length=159, Percent_Identity=33.9622641509434, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1787541, Length=161, Percent_Identity=32.9192546583851, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI87081974, Length=156, Percent_Identity=30.1282051282051, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1788381, Length=158, Percent_Identity=30.379746835443, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1788956, Length=234, Percent_Identity=33.3333333333333, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI1788085, Length=177, Percent_Identity=27.1186440677966, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1787056, Length=161, Percent_Identity=36.0248447204969, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 41973; Mature: 41841

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEEQPDNTDDQIVLPPIPPNEEERLRELESFQILDTLPEQIFEDIVRIAAYICQTPIAL
CCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHH
VNLVDRHRQWSMARLGFSDGEVERTLTFCAFTINDPDHVMVVPDATRDPRFASNPYVRRE
HHHHHHHHHHHHHHCCCCCCHHHHEEEEEEEEECCCCEEEECCCCCCCCCCCCCCCCCCC
PYIRFYAGAPLVTSHGYALGTVCVIDLQPRQLSPEQLEMLRLLSRQVVTQLEIRRDLLRL
CCEEEEECCCEEECCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
EQRLLQREQEYLSLNAYLQSLEDKLAAAQQQSLTDPLTGVFNRRGFALRLSEEIERARRY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCEEEEHHHHHHHHHHH
QVTLALLLIDVDHFKQYNDTFGHLAGDEVLRMIAQLLSEGCCKHDIVARYGGEEFAVILP
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHCCCCEEEEEEE
ATAGDGAQVLGERLRRLVQMAAWPLHPLTISVGGAVLQPEMTDEHDLIARADSALYQAKA
CCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCEEECCCCCCCHHHHHHHHHHHHHHHH
QGRNTCVIIA
CCCCEEEEEC
>Mature Secondary Structure 
TEEQPDNTDDQIVLPPIPPNEEERLRELESFQILDTLPEQIFEDIVRIAAYICQTPIAL
CCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHH
VNLVDRHRQWSMARLGFSDGEVERTLTFCAFTINDPDHVMVVPDATRDPRFASNPYVRRE
HHHHHHHHHHHHHHCCCCCCHHHHEEEEEEEEECCCCEEEECCCCCCCCCCCCCCCCCCC
PYIRFYAGAPLVTSHGYALGTVCVIDLQPRQLSPEQLEMLRLLSRQVVTQLEIRRDLLRL
CCEEEEECCCEEECCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
EQRLLQREQEYLSLNAYLQSLEDKLAAAQQQSLTDPLTGVFNRRGFALRLSEEIERARRY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCEEEEHHHHHHHHHHH
QVTLALLLIDVDHFKQYNDTFGHLAGDEVLRMIAQLLSEGCCKHDIVARYGGEEFAVILP
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHCCCCEEEEEEE
ATAGDGAQVLGERLRRLVQMAAWPLHPLTISVGGAVLQPEMTDEHDLIARADSALYQAKA
CCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCEEECCCCCCCHHHHHHHHHHHHHHHH
QGRNTCVIIA
CCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA