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The map label for this gene is sigA [H]

Identifier: 222526547

GI number: 222526547

Start: 4129348

End: 4130493

Strand: Reverse

Name: sigA [H]

Synonym: Chy400_3314

Alternate gene names: 222526547

Gene position: 4130493-4129348 (Counterclockwise)

Preceding gene: 222526548

Following gene: 222526546

Centisome position: 78.39

GC content: 54.8

Gene sequence:

>1146_bases
ATGATCGAGCAATTGCTCGCCGCAGCTCGTGTGCGTGGCTACATCACCCACGCCGACATTCTTGCTACTTTTCCAAACCC
AGAGCACGACATTGCTGAGATCGATCAGCTTTATGCGATGCTGCAAGCTGAAGGGATCAAAGTAATCGAGTCAAGTGATG
AACTCGATGGCCCGACCGATTTTGAACCTGATCCAGATGCTGATCTCATTGCTGATTTGCCTGATTTGGGCGATATTGCA
TTTGATGACCCGGTTCGCATGTACTTGCAAGAGATCGGGCAGGTACCGTTGCTGACTGCCGAACAAGAGGTTGAACTGGC
AAAAGCAATGGAAGCAGGTGCTGCTGCTCGCCAGCGCCTTGATCGCGAAGAATACACCTCGGCCCGCGAGCGGTTCGAGC
TTGAACGGGCAGTCCAGCAGGGACAGGATGCCCGCCATCATCTCATTCAGGCTAATCTGCGCCTGGTCGTCAGTATTGCC
AAGAAGTACACGTCCTACGGCCTGACGATGATGGATCTCGTCCAAGAGGGAAATATTGGCCTGATGCGGGCAGTTGAGAA
ATTTGATTACAAAAAGGGGCATAAGTTTAGCACTTACGCAACGTGGTGGATCCGTCAGGCGATTACTCGTGCGATTGCCG
ATCAGAGCCGCACGATCCGTCTCCCCGTCCATATGGGTGAGGCAATTAGTCAGGTGAAGCGCACGTCTCATCGCCTCCAG
CAGACGATGCAGCGTGAGCCAACGCCGGAAGAGATCGCCGATGCAATGGGTATCTCTGCTGGTAAAGTACGGCGCACGCT
TGAGGCGAGCATGCATCCCCTTTCCCTGGAGATGCCGGTCGGGCAAGAGGGTGAAGGACGGATGGGCGATTTTATTGAGG
ATGATCGTATTTCCACTCCCGCCGAAGCGGCTGCTGCCTCGTTGCTGCGCGAGCAGCTCGAAGAGGTTTTGATGAAGCTG
CCCGAACGCGAGCGGAAGATCATTCAATTGCGCTACGGTCTCAAAGACGGTCGTTACCGCACGCTCGAAGAGGTCGGTAT
CGAGTTTGGGATTACCCGCGAGCGCATTCGCCAGATTGAAGCAGTTGCCCTTCGCAAGCTCCGCCATCCCCATCTCGGTA
AGAAGCTCCGCGGCTATCTGGATTAA

Upstream 100 bases:

>100_bases
TCTCATCAACGGCCTAGTGATGGTCGTCACGCCAGCCTGACGCCATCAACAGAGTACTGGTTGAACGATGTTGAGCAAAG
CTACACCGGAGTCAGAGGCA

Downstream 100 bases:

>100_bases
GCTCTTTATCGCTTGTGTGTAAGCCTTAATAGCCCCCGTGACGATTGCACGGGGGCTAGTATGTACCTGTGACTGTTATG
CAACGCCTCGATGAAGCACA

Product: RpoD subfamily RNA polymerase sigma-70 subunit

Products: NA

Alternate protein names: Sigma-43; Sigma-A [H]

Number of amino acids: Translated: 381; Mature: 381

Protein sequence:

>381_residues
MIEQLLAAARVRGYITHADILATFPNPEHDIAEIDQLYAMLQAEGIKVIESSDELDGPTDFEPDPDADLIADLPDLGDIA
FDDPVRMYLQEIGQVPLLTAEQEVELAKAMEAGAAARQRLDREEYTSARERFELERAVQQGQDARHHLIQANLRLVVSIA
KKYTSYGLTMMDLVQEGNIGLMRAVEKFDYKKGHKFSTYATWWIRQAITRAIADQSRTIRLPVHMGEAISQVKRTSHRLQ
QTMQREPTPEEIADAMGISAGKVRRTLEASMHPLSLEMPVGQEGEGRMGDFIEDDRISTPAEAAAASLLREQLEEVLMKL
PERERKIIQLRYGLKDGRYRTLEEVGIEFGITRERIRQIEAVALRKLRHPHLGKKLRGYLD

Sequences:

>Translated_381_residues
MIEQLLAAARVRGYITHADILATFPNPEHDIAEIDQLYAMLQAEGIKVIESSDELDGPTDFEPDPDADLIADLPDLGDIA
FDDPVRMYLQEIGQVPLLTAEQEVELAKAMEAGAAARQRLDREEYTSARERFELERAVQQGQDARHHLIQANLRLVVSIA
KKYTSYGLTMMDLVQEGNIGLMRAVEKFDYKKGHKFSTYATWWIRQAITRAIADQSRTIRLPVHMGEAISQVKRTSHRLQ
QTMQREPTPEEIADAMGISAGKVRRTLEASMHPLSLEMPVGQEGEGRMGDFIEDDRISTPAEAAAASLLREQLEEVLMKL
PERERKIIQLRYGLKDGRYRTLEEVGIEFGITRERIRQIEAVALRKLRHPHLGKKLRGYLD
>Mature_381_residues
MIEQLLAAARVRGYITHADILATFPNPEHDIAEIDQLYAMLQAEGIKVIESSDELDGPTDFEPDPDADLIADLPDLGDIA
FDDPVRMYLQEIGQVPLLTAEQEVELAKAMEAGAAARQRLDREEYTSARERFELERAVQQGQDARHHLIQANLRLVVSIA
KKYTSYGLTMMDLVQEGNIGLMRAVEKFDYKKGHKFSTYATWWIRQAITRAIADQSRTIRLPVHMGEAISQVKRTSHRLQ
QTMQREPTPEEIADAMGISAGKVRRTLEASMHPLSLEMPVGQEGEGRMGDFIEDDRISTPAEAAAASLLREQLEEVLMKL
PERERKIIQLRYGLKDGRYRTLEEVGIEFGITRERIRQIEAVALRKLRHPHLGKKLRGYLD

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This is the primary sigma factor of this bacterium [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=293, Percent_Identity=48.122866894198, Blast_Score=279, Evalue=2e-76,
Organism=Escherichia coli, GI1789098, Length=286, Percent_Identity=38.8111888111888, Blast_Score=208, Evalue=5e-55,
Organism=Escherichia coli, GI1789871, Length=243, Percent_Identity=30.4526748971193, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1788231, Length=233, Percent_Identity=27.8969957081545, Blast_Score=75, Evalue=8e-15,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR007127
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012760
- InterPro:   IPR011991 [H]

Pfam domain/function: PF03979 Sigma70_r1_1; PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 43235; Mature: 43235

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEQLLAAARVRGYITHADILATFPNPEHDIAEIDQLYAMLQAEGIKVIESSDELDGPTD
CHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCC
FEPDPDADLIADLPDLGDIAFDDPVRMYLQEIGQVPLLTAEQEVELAKAMEAGAAARQRL
CCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHC
DREEYTSARERFELERAVQQGQDARHHLIQANLRLVVSIAKKYTSYGLTMMDLVQEGNIG
CHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCHH
LMRAVEKFDYKKGHKFSTYATWWIRQAITRAIADQSRTIRLPVHMGEAISQVKRTSHRLQ
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHH
QTMQREPTPEEIADAMGISAGKVRRTLEASMHPLSLEMPVGQEGEGRMGDFIEDDRISTP
HHHHCCCCHHHHHHHHCCCHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCCCCCC
AEAAAASLLREQLEEVLMKLPERERKIIQLRYGLKDGRYRTLEEVGIEFGITRERIRQIE
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHCHHCCCCHHHHHHHH
AVALRKLRHPHLGKKLRGYLD
HHHHHHHCCCCCCHHHHCCCC
>Mature Secondary Structure
MIEQLLAAARVRGYITHADILATFPNPEHDIAEIDQLYAMLQAEGIKVIESSDELDGPTD
CHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCC
FEPDPDADLIADLPDLGDIAFDDPVRMYLQEIGQVPLLTAEQEVELAKAMEAGAAARQRL
CCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHC
DREEYTSARERFELERAVQQGQDARHHLIQANLRLVVSIAKKYTSYGLTMMDLVQEGNIG
CHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCHH
LMRAVEKFDYKKGHKFSTYATWWIRQAITRAIADQSRTIRLPVHMGEAISQVKRTSHRLQ
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHH
QTMQREPTPEEIADAMGISAGKVRRTLEASMHPLSLEMPVGQEGEGRMGDFIEDDRISTP
HHHHCCCCHHHHHHHHCCCHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCCCCCC
AEAAAASLLREQLEEVLMKLPERERKIIQLRYGLKDGRYRTLEEVGIEFGITRERIRQIE
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHCHHCCCCHHHHHHHH
AVALRKLRHPHLGKKLRGYLD
HHHHHHHCCCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]