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The map label for this gene is gyrB [H]

Identifier: 222526487

GI number: 222526487

Start: 4057609

End: 4059519

Strand: Reverse

Name: gyrB [H]

Synonym: Chy400_3254

Alternate gene names: 222526487

Gene position: 4059519-4057609 (Counterclockwise)

Preceding gene: 222526488

Following gene: 222526486

Centisome position: 77.05

GC content: 54.95

Gene sequence:

>1911_bases
ATGACAAGCGAGACACCGACAACCTACGATGAAAGTCAGATTCAGGTGCTTGAGGGCATGGAAGCCGTGCGCAAGCGCCC
CGGTATGTATATTGGCCCAACCGACATCAATGGCCTGCATACGATGGTGCGTGAAGTGGTTGACAATTCGGTCGATGAGG
TGATGGCCGGTCGTGCAACCAGCGTGAGCGTTACCATTCATCGTGATGGCTCGGTGACCGTCAGTGATGATGGGTCGGGG
ATTCCGGTCGGTATTCATCCCAAAGAGGGGATCAGCACGCTGACCCTGGTGATGACGCGCCTGCACGCCGGTGGTAAATT
CGGCAGCGGTGGCTATAAGGTTTCGTCTGGCCTGCACGGCGTCGGCGTCTCGGCTGTTAATGCCCTGTCGGCTTATATGC
GAGTAGACGTGTATCGCAATGGCCGCCACTACTACCAGGAATATCGGGCCGGTGTGCCGGTCACCGATGTAATCGATCTC
GAACCAACCGACCGCCACGGTACGACGACGCGCTTTTTGCCCGACACATCCATCATTCAAACGCTTGACTACAATTTCGA
TACGCTGGCCCAGCGCTTCCGCGAGATGAGCTATCTCAATAAGGGGTTGCGCTTTAAGTTTGTTGATGAACGGACAAACC
GTGAACTCAACTTCTATTTTGAAGGCGGAATCGTTTCCTATGTCCGCCATCTGAACAAGGAAAAGACGGTACTCCATCGT
CAACCGTTTTATGTCGAGCGGATTGTTGACGATGTGATGGTTGAGGTGGCGCTCCAGTACACCGATTCGTTCGATACCGA
TAACGTCTACACTTTTGCCAATAACATCAACAACGCCGATGGTGGATCGCACCTGTCAGGATTTCGCACCGCACTGACCC
GTGTCATCAACAATTATGCGCGGGCAAAGGGGCTGTTGAAAGACAACGAAAGCAACCTGACCGGTGATGATGTGCGCGAG
GGCTTGACGGCGGTTATCAGCGTTAAGCTGAAAGACCCTCAGTTCTCGTCGCAAACCAAAGAGAAGCTGGTCAGCCCGGA
GGCGGCCAGTGCGGTGGCGACGGTGTTCAGCGATGCGTTCAGTGCCTGGCTCGACGAGAATCCCACTGAAGCGCGGCGTA
TTATCGAAAAAGCTATTTCTGCTGCGCGTACCAGGCTGGCCGTACAGAAGGTACGCGAGACAGCACGCAAGAGCGCAATG
GAGGGGTTCTCGCTCCCCGGTAAGCTCGCCGATTGTTCAGACCCCAATCCGGCCCGCTGTGAACTCTACATTGTTGAGGG
TGATAGCGCAGGCGGCACGGCGAAACAGGGACGTGACCGGCGCTTTCAGGCGATTTTGCCGTTGCGCGGGAAGATTCTTA
ATGTCGAAAAGAGTCGGCTTGATCGCATGCTCTCGAATGCCGAGGTGCGTGCGCTAATTACCGCCATCGGTACGTCTATC
GGTGATCAGTTTGATCTCAGTAAACTGCGCTATCACCGCATTTTCCTGATGACCGACGCCGATGTTGACGGCAGCCATAT
CCGCACGCTGCTGTTGACCTTCTTCTTCCGGCATATGCGACCGCTCATTACCAACGGTCATTTGTTCATCGCCCAACCGC
CACTCTACCGGATCAAACACGGTCGCGAGCAGGTCTACACCTACTCTGATGCTGAGCGTGATGCGTATCTGAGCAAATTG
CCACCCGGTACCAAGGTAGAAATCCAGCGTTACAAAGGGCTGGGTGAAATGAACGCCGAACAGCTCTGGGAGACGACGAT
GAATCCGCAAAACCGGGTTATTCTTCAGGTGACGCTGGAAGATGCCGCACGCGCCGATGAGACGTTTACGATGTTGATGG
GAGACCTGGTGCCACCACGCCGGCGGTTTATTCAAACCCACGCGAACGAGGTGGTTGAGCTGGATATTTGA

Upstream 100 bases:

>100_bases
GGCATTCGTGCCGCTCGTTTACAGTTTTATCGCTATCACGCCGCCACGCTATTGCCGGCGTAGGGCGAAACACAATGTGA
CACTATAAGGAAAACAGACT

Downstream 100 bases:

>100_bases
GAGGATGTTCAGCGGGTACGTGAACGTCGTGTAGGGTTGTTACTGTTGCACCACCGAGCCACAGAGGACCCAGAGGTAGT
ACACTCTGCCGTGTTCTCTG

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 636; Mature: 635

Protein sequence:

>636_residues
MTSETPTTYDESQIQVLEGMEAVRKRPGMYIGPTDINGLHTMVREVVDNSVDEVMAGRATSVSVTIHRDGSVTVSDDGSG
IPVGIHPKEGISTLTLVMTRLHAGGKFGSGGYKVSSGLHGVGVSAVNALSAYMRVDVYRNGRHYYQEYRAGVPVTDVIDL
EPTDRHGTTTRFLPDTSIIQTLDYNFDTLAQRFREMSYLNKGLRFKFVDERTNRELNFYFEGGIVSYVRHLNKEKTVLHR
QPFYVERIVDDVMVEVALQYTDSFDTDNVYTFANNINNADGGSHLSGFRTALTRVINNYARAKGLLKDNESNLTGDDVRE
GLTAVISVKLKDPQFSSQTKEKLVSPEAASAVATVFSDAFSAWLDENPTEARRIIEKAISAARTRLAVQKVRETARKSAM
EGFSLPGKLADCSDPNPARCELYIVEGDSAGGTAKQGRDRRFQAILPLRGKILNVEKSRLDRMLSNAEVRALITAIGTSI
GDQFDLSKLRYHRIFLMTDADVDGSHIRTLLLTFFFRHMRPLITNGHLFIAQPPLYRIKHGREQVYTYSDAERDAYLSKL
PPGTKVEIQRYKGLGEMNAEQLWETTMNPQNRVILQVTLEDAARADETFTMLMGDLVPPRRRFIQTHANEVVELDI

Sequences:

>Translated_636_residues
MTSETPTTYDESQIQVLEGMEAVRKRPGMYIGPTDINGLHTMVREVVDNSVDEVMAGRATSVSVTIHRDGSVTVSDDGSG
IPVGIHPKEGISTLTLVMTRLHAGGKFGSGGYKVSSGLHGVGVSAVNALSAYMRVDVYRNGRHYYQEYRAGVPVTDVIDL
EPTDRHGTTTRFLPDTSIIQTLDYNFDTLAQRFREMSYLNKGLRFKFVDERTNRELNFYFEGGIVSYVRHLNKEKTVLHR
QPFYVERIVDDVMVEVALQYTDSFDTDNVYTFANNINNADGGSHLSGFRTALTRVINNYARAKGLLKDNESNLTGDDVRE
GLTAVISVKLKDPQFSSQTKEKLVSPEAASAVATVFSDAFSAWLDENPTEARRIIEKAISAARTRLAVQKVRETARKSAM
EGFSLPGKLADCSDPNPARCELYIVEGDSAGGTAKQGRDRRFQAILPLRGKILNVEKSRLDRMLSNAEVRALITAIGTSI
GDQFDLSKLRYHRIFLMTDADVDGSHIRTLLLTFFFRHMRPLITNGHLFIAQPPLYRIKHGREQVYTYSDAERDAYLSKL
PPGTKVEIQRYKGLGEMNAEQLWETTMNPQNRVILQVTLEDAARADETFTMLMGDLVPPRRRFIQTHANEVVELDI
>Mature_635_residues
TSETPTTYDESQIQVLEGMEAVRKRPGMYIGPTDINGLHTMVREVVDNSVDEVMAGRATSVSVTIHRDGSVTVSDDGSGI
PVGIHPKEGISTLTLVMTRLHAGGKFGSGGYKVSSGLHGVGVSAVNALSAYMRVDVYRNGRHYYQEYRAGVPVTDVIDLE
PTDRHGTTTRFLPDTSIIQTLDYNFDTLAQRFREMSYLNKGLRFKFVDERTNRELNFYFEGGIVSYVRHLNKEKTVLHRQ
PFYVERIVDDVMVEVALQYTDSFDTDNVYTFANNINNADGGSHLSGFRTALTRVINNYARAKGLLKDNESNLTGDDVREG
LTAVISVKLKDPQFSSQTKEKLVSPEAASAVATVFSDAFSAWLDENPTEARRIIEKAISAARTRLAVQKVRETARKSAME
GFSLPGKLADCSDPNPARCELYIVEGDSAGGTAKQGRDRRFQAILPLRGKILNVEKSRLDRMLSNAEVRALITAIGTSIG
DQFDLSKLRYHRIFLMTDADVDGSHIRTLLLTFFFRHMRPLITNGHLFIAQPPLYRIKHGREQVYTYSDAERDAYLSKLP
PGTKVEIQRYKGLGEMNAEQLWETTMNPQNRVILQVTLEDAARADETFTMLMGDLVPPRRRFIQTHANEVVELDI

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI19913406, Length=582, Percent_Identity=24.3986254295533, Blast_Score=136, Evalue=7e-32,
Organism=Homo sapiens, GI19913408, Length=569, Percent_Identity=24.6045694200351, Blast_Score=133, Evalue=5e-31,
Organism=Escherichia coli, GI48994957, Length=553, Percent_Identity=53.5262206148282, Blast_Score=579, Evalue=1e-166,
Organism=Escherichia coli, GI1789408, Length=634, Percent_Identity=39.4321766561514, Blast_Score=448, Evalue=1e-127,
Organism=Caenorhabditis elegans, GI212645845, Length=540, Percent_Identity=25.7407407407407, Blast_Score=147, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17535065, Length=565, Percent_Identity=24.9557522123894, Blast_Score=139, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI212645657, Length=186, Percent_Identity=30.1075268817204, Blast_Score=84, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6324241, Length=658, Percent_Identity=23.7082066869301, Blast_Score=118, Evalue=2e-27,
Organism=Drosophila melanogaster, GI17136538, Length=624, Percent_Identity=24.3589743589744, Blast_Score=136, Evalue=4e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR006171 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 71199; Mature: 71067

Theoretical pI: Translated: 6.97; Mature: 6.97

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSETPTTYDESQIQVLEGMEAVRKRPGMYIGPTDINGLHTMVREVVDNSVDEVMAGRAT
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCEECCCCCCHHHHHHHHHHCCCHHHHHCCCCE
SVSVTIHRDGSVTVSDDGSGIPVGIHPKEGISTLTLVMTRLHAGGKFGSGGYKVSSGLHG
EEEEEEEECCEEEECCCCCCCEEECCCCCCHHHHHHHHHHHHCCCCCCCCCEEECCCCCC
VGVSAVNALSAYMRVDVYRNGRHYYQEYRAGVPVTDVIDLEPTDRHGTTTRFLPDTSIIQ
CCHHHHHHHHHHHHEEEEECCHHHHHHHHCCCCCEEEEECCCCCCCCCEEEECCCHHHHH
TLDYNFDTLAQRFREMSYLNKGLRFKFVDERTNRELNFYFEGGIVSYVRHLNKEKTVLHR
HHCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEECCCHHHHHHHCCCHHHHHHC
QPFYVERIVDDVMVEVALQYTDSFDTDNVYTFANNINNADGGSHLSGFRTALTRVINNYA
CCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHH
RAKGLLKDNESNLTGDDVREGLTAVISVKLKDPQFSSQTKEKLVSPEAASAVATVFSDAF
HHCCCCCCCCCCCCHHHHHHCCEEEEEEEECCCCCCCHHHHHHCCCHHHHHHHHHHHHHH
SAWLDENPTEARRIIEKAISAARTRLAVQKVRETARKSAMEGFSLPGKLADCSDPNPARC
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEE
ELYIVEGDSAGGTAKQGRDRRFQAILPLRGKILNVEKSRLDRMLSNAEVRALITAIGTSI
EEEEEECCCCCCCCCCCCCCCEEEECCCCCEEECCHHHHHHHHHCCHHHHHHHHHHHHHC
GDQFDLSKLRYHRIFLMTDADVDGSHIRTLLLTFFFRHMRPLITNGHLFIAQPPLYRIKH
CCCCCHHHHEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHC
GREQVYTYSDAERDAYLSKLPPGTKVEIQRYKGLGEMNAEQLWETTMNPQNRVILQVTLE
CCHHEEEECCCHHHHHHHHCCCCCCEEHHHHCCCCCCCHHHHHHHHCCCCCEEEEEEEEC
DAARADETFTMLMGDLVPPRRRFIQTHANEVVELDI
HHCCCCHHHHHHHHCCCCHHHHHHHHHCCCEEEECC
>Mature Secondary Structure 
TSETPTTYDESQIQVLEGMEAVRKRPGMYIGPTDINGLHTMVREVVDNSVDEVMAGRAT
CCCCCCCCCHHHHHHHHHHHHHHHCCCCEECCCCCCHHHHHHHHHHCCCHHHHHCCCCE
SVSVTIHRDGSVTVSDDGSGIPVGIHPKEGISTLTLVMTRLHAGGKFGSGGYKVSSGLHG
EEEEEEEECCEEEECCCCCCCEEECCCCCCHHHHHHHHHHHHCCCCCCCCCEEECCCCCC
VGVSAVNALSAYMRVDVYRNGRHYYQEYRAGVPVTDVIDLEPTDRHGTTTRFLPDTSIIQ
CCHHHHHHHHHHHHEEEEECCHHHHHHHHCCCCCEEEEECCCCCCCCCEEEECCCHHHHH
TLDYNFDTLAQRFREMSYLNKGLRFKFVDERTNRELNFYFEGGIVSYVRHLNKEKTVLHR
HHCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEECCCHHHHHHHCCCHHHHHHC
QPFYVERIVDDVMVEVALQYTDSFDTDNVYTFANNINNADGGSHLSGFRTALTRVINNYA
CCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHH
RAKGLLKDNESNLTGDDVREGLTAVISVKLKDPQFSSQTKEKLVSPEAASAVATVFSDAF
HHCCCCCCCCCCCCHHHHHHCCEEEEEEEECCCCCCCHHHHHHCCCHHHHHHHHHHHHHH
SAWLDENPTEARRIIEKAISAARTRLAVQKVRETARKSAMEGFSLPGKLADCSDPNPARC
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEE
ELYIVEGDSAGGTAKQGRDRRFQAILPLRGKILNVEKSRLDRMLSNAEVRALITAIGTSI
EEEEEECCCCCCCCCCCCCCCEEEECCCCCEEECCHHHHHHHHHCCHHHHHHHHHHHHHC
GDQFDLSKLRYHRIFLMTDADVDGSHIRTLLLTFFFRHMRPLITNGHLFIAQPPLYRIKH
CCCCCHHHHEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHC
GREQVYTYSDAERDAYLSKLPPGTKVEIQRYKGLGEMNAEQLWETTMNPQNRVILQVTLE
CCHHEEEECCCHHHHHHHHCCCCCCEEHHHHCCCCCCCHHHHHHHHCCCCCEEEEEEEEC
DAARADETFTMLMGDLVPPRRRFIQTHANEVVELDI
HHCCCCHHHHHHHHCCCCHHHHHHHHHCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2987847; 7584024; 9384377 [H]