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The map label for this gene is cobB2 [H]

Identifier: 222526409

GI number: 222526409

Start: 3936605

End: 3937369

Strand: Reverse

Name: cobB2 [H]

Synonym: Chy400_3175

Alternate gene names: 222526409

Gene position: 3937369-3936605 (Counterclockwise)

Preceding gene: 222526410

Following gene: 222526407

Centisome position: 74.73

GC content: 58.82

Gene sequence:

>765_bases
ATGAACGATCTCGATGATGTCATCACGATACTGGCTACAGCTCTATCCACGGCCCGTCATGTCACCGTGCTGACCGGTGC
CGGCGTATCGGCTGAAAGTGGTATTCCAACGTTTCGTGATGCTCAGACCGGTCTCTGGTCACATTTTGACCCGGAGGAAC
TGGCTTCACCGGCAGGTTTTGCCCGCAACCCAGCACTGGTCTGGCGCTGGTACGCCGAGCGACGGGTGAAAGCCTGTACT
GCCCAGCCTAACCCGGCCCACCATGCCCTGGCCGATCTGGCGACCCTGGTACCAAGGCTCACGCTGGTTACGCAGAATAT
TGACGGATTACACCAGCGGGCCGGTAGCCCACAGGTGATCGAACTGCACGGCAACATCCACCGTGCCCGCTGTACGGTCG
ATGGTTCAATCCATACCACCTGGGATTACGACGAAGAGCTACCGCAGTGCCCCAACTGCGGCGCATTGTTGCGCCCTGAT
GTGGTCTGGTTTGGCGAATATCTGCCACCTGGTGCCCTGGAAGCTGCTTATGCTGCAACCCTCGACTGCGATGTCTTCTG
CTCGATTGGGACATCAGGGGTTGTCGAACCGGCAGCCTCACTACCACGCATTGCCCTGTCACGGGGCGCGACTGTGTTGA
TCCTCAACCTTGAACAAACCACTACCGCTCGCTCACCACTGTTCACCGTGTACGGAAAGGCCGGTGAAGTGTTACCGCGC
CTGGTAGCAGCGGTGCGATCAATGCGTCGGCAACAGGGTGAATGA

Upstream 100 bases:

>100_bases
GGCCCCTACCTGTGGCATCTGGCACGTCACCTTTACCAACCAGCGGGCCAGCCGATTCGATCTGGTGTTCAAGCCGTCCT
GAACGTAGCGGAGGCCGGTC

Downstream 100 bases:

>100_bases
ATGGCAATACTGGCTGTGTGTTCACCTGGAGCAACTCTCTCTGTGGGTCGCTTGTGTACGACCAATAAGATGGCAAATAC
GACACGTTGATCTCGCGCCT

Product: Silent information regulator protein Sir2

Products: NA

Alternate protein names: Regulatory protein SIR2 homolog 2 [H]

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MNDLDDVITILATALSTARHVTVLTGAGVSAESGIPTFRDAQTGLWSHFDPEELASPAGFARNPALVWRWYAERRVKACT
AQPNPAHHALADLATLVPRLTLVTQNIDGLHQRAGSPQVIELHGNIHRARCTVDGSIHTTWDYDEELPQCPNCGALLRPD
VVWFGEYLPPGALEAAYAATLDCDVFCSIGTSGVVEPAASLPRIALSRGATVLILNLEQTTTARSPLFTVYGKAGEVLPR
LVAAVRSMRRQQGE

Sequences:

>Translated_254_residues
MNDLDDVITILATALSTARHVTVLTGAGVSAESGIPTFRDAQTGLWSHFDPEELASPAGFARNPALVWRWYAERRVKACT
AQPNPAHHALADLATLVPRLTLVTQNIDGLHQRAGSPQVIELHGNIHRARCTVDGSIHTTWDYDEELPQCPNCGALLRPD
VVWFGEYLPPGALEAAYAATLDCDVFCSIGTSGVVEPAASLPRIALSRGATVLILNLEQTTTARSPLFTVYGKAGEVLPR
LVAAVRSMRRQQGE
>Mature_254_residues
MNDLDDVITILATALSTARHVTVLTGAGVSAESGIPTFRDAQTGLWSHFDPEELASPAGFARNPALVWRWYAERRVKACT
AQPNPAHHALADLATLVPRLTLVTQNIDGLHQRAGSPQVIELHGNIHRARCTVDGSIHTTWDYDEELPQCPNCGALLRPD
VVWFGEYLPPGALEAAYAATLDCDVFCSIGTSGVVEPAASLPRIALSRGATVLILNLEQTTTARSPLFTVYGKAGEVLPR
LVAAVRSMRRQQGE

Specific function: Modulates the activity of chromatin proteins, thereby having an effect on transcription per subunit [H]

COG id: COG0846

COG function: function code K; NAD-dependent protein deacetylases, SIR2 family

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 deacetylase sirtuin-type domain [H]

Homologues:

Organism=Homo sapiens, GI300795542, Length=243, Percent_Identity=38.6831275720165, Blast_Score=168, Evalue=5e-42,
Organism=Homo sapiens, GI6912664, Length=257, Percent_Identity=36.9649805447471, Blast_Score=166, Evalue=2e-41,
Organism=Homo sapiens, GI13787215, Length=234, Percent_Identity=38.034188034188, Blast_Score=159, Evalue=2e-39,
Organism=Homo sapiens, GI6912662, Length=269, Percent_Identity=27.8810408921933, Blast_Score=112, Evalue=4e-25,
Organism=Homo sapiens, GI6912660, Length=226, Percent_Identity=32.7433628318584, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI13775602, Length=198, Percent_Identity=31.3131313131313, Blast_Score=93, Evalue=2e-19,
Organism=Homo sapiens, GI63054862, Length=206, Percent_Identity=33.495145631068, Blast_Score=92, Evalue=3e-19,
Organism=Homo sapiens, GI13775600, Length=198, Percent_Identity=31.3131313131313, Blast_Score=92, Evalue=4e-19,
Organism=Homo sapiens, GI7657575, Length=220, Percent_Identity=29.0909090909091, Blast_Score=88, Evalue=7e-18,
Organism=Homo sapiens, GI300797705, Length=190, Percent_Identity=30, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI7706712, Length=265, Percent_Identity=26.7924528301887, Blast_Score=75, Evalue=5e-14,
Organism=Escherichia coli, GI308199517, Length=228, Percent_Identity=45.1754385964912, Blast_Score=162, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI71990482, Length=278, Percent_Identity=25.8992805755396, Blast_Score=101, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17567771, Length=275, Percent_Identity=24.7272727272727, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI71990487, Length=280, Percent_Identity=25.3571428571429, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17541892, Length=217, Percent_Identity=28.5714285714286, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24648389, Length=199, Percent_Identity=32.6633165829146, Blast_Score=101, Evalue=4e-22,
Organism=Drosophila melanogaster, GI28571445, Length=276, Percent_Identity=27.8985507246377, Blast_Score=101, Evalue=5e-22,
Organism=Drosophila melanogaster, GI17137536, Length=259, Percent_Identity=30.1158301158301, Blast_Score=92, Evalue=3e-19,
Organism=Drosophila melanogaster, GI28571443, Length=220, Percent_Identity=25.9090909090909, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI28571441, Length=220, Percent_Identity=25.9090909090909, Blast_Score=73, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003000 [H]

Pfam domain/function: PF02146 SIR2 [H]

EC number: 3.5.1.- [C]

Molecular weight: Translated: 27382; Mature: 27382

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: PS50305 SIRTUIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDLDDVITILATALSTARHVTVLTGAGVSAESGIPTFRDAQTGLWSHFDPEELASPAGF
CCHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCC
ARNPALVWRWYAERRVKACTAQPNPAHHALADLATLVPRLTLVTQNIDGLHQRAGSPQVI
CCCCHHEEHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCEE
ELHGNIHRARCTVDGSIHTTWDYDEELPQCPNCGALLRPDVVWFGEYLPPGALEAAYAAT
EECCCEEEEEEEECCCEEECCCCHHCCCCCCCCCCCCCCCCEEECCCCCCCHHHHHHHHH
LDCDVFCSIGTSGVVEPAASLPRIALSRGATVLILNLEQTTTARSPLFTVYGKAGEVLPR
CCCEEEEECCCCCCCCHHHHCCHHHHHCCCEEEEEECCCCCCCCCCEEEEECCHHHHHHH
LVAAVRSMRRQQGE
HHHHHHHHHHHCCC
>Mature Secondary Structure
MNDLDDVITILATALSTARHVTVLTGAGVSAESGIPTFRDAQTGLWSHFDPEELASPAGF
CCHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCC
ARNPALVWRWYAERRVKACTAQPNPAHHALADLATLVPRLTLVTQNIDGLHQRAGSPQVI
CCCCHHEEHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCEE
ELHGNIHRARCTVDGSIHTTWDYDEELPQCPNCGALLRPDVVWFGEYLPPGALEAAYAAT
EECCCEEEEEEEECCCEEECCCCHHCCCCCCCCCCCCCCCCEEECCCCCCCHHHHHHHHH
LDCDVFCSIGTSGVVEPAASLPRIALSRGATVLILNLEQTTTARSPLFTVYGKAGEVLPR
CCCEEEEECCCCCCCCHHHHCCHHHHHCCCEEEEEECCCCCCCCCCEEEEECCHHHHHHH
LVAAVRSMRRQQGE
HHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11792869 [H]