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The map label for this gene is dehH109 [H]

Identifier: 222526353

GI number: 222526353

Start: 3859648

End: 3860328

Strand: Reverse

Name: dehH109 [H]

Synonym: Chy400_3119

Alternate gene names: 222526353

Gene position: 3860328-3859648 (Counterclockwise)

Preceding gene: 222526355

Following gene: 222526342

Centisome position: 73.27

GC content: 58.44

Gene sequence:

>681_bases
ATGCCACGTGTTTGTGTCTTCGACGTGAATGAGACCTTGCTCGACCTGCGTGCCCTCGATCCTATGTTTGAGCAGGCGTT
TGGCGACCCTGGCGTGCGGCACGAGTGGTTTCAGCAGGTGATTCACCTGGCAATGGTGGTCACCATTACGGGAAGCTATG
TTCCATTCGCGCAGATTGGCGATGCCGCACTGACAATGACAGCCGCACGCCGTGGTGTAAAGCTGAGCAGTAGCGGTCGG
CAACAGATTCTGGCTGGTATGCGGCAACTGCCTCCGCATCCCGATGTACAACCGGCACTCGAACGCTTGCAGGCCAACGG
GATCAGGCTGGCAACACTCACGAATTCAAATGTCGAACTGGCCGAGGCTCAACTTGCTTTTGCCGGCCTGCGTGACTTCT
TTGAGGTGCGTCTGTCGGTCGAGGAAGTTCGCCGTTTGAAACCGGCGCCGGAGGTGTATCAGATGGCGGCTCATCGGCTG
GGTGTGGCAATGGATCAGGTACGGTTGATTGCCGCCCATGCCTGGGACGTAGCAGGAGCCTTGCGTGCCGGTTGTGCCGC
TGCTTTTGTCGCCCGTCCAGGTATGGTACTCGACCCATTGGCTCCACCGCCGGATGTGGTAGGACATGATCTGGCCGCGG
TTGCAGATCAAATCCTGGTCATAGAGAGGCAGGCGGCATAA

Upstream 100 bases:

>100_bases
CTTTACTGGTATGACGCACACCCACAGCAATTCGTTCCATTCAGGGCAGCCGTATCCGAGGCTGTATAATATGCATCTAC
TATCAAACAGGAGAACTGCT

Downstream 100 bases:

>100_bases
CACGCTCATCTATGTTGTGAGTGATCAAACGCGGGTGAACTTATTATGACATTGACAGCGAATTTTTCTATGAATGGTCT
CACGCAAGAGGCGTTCGGCA

Product: haloacid dehalogenase I

Products: NA

Alternate protein names: 2-haloalkanoic acid dehalogenase H-109; Halocarboxylic acid halidohydrolase H-109; L-2-haloacid dehalogenase H-109 [H]

Number of amino acids: Translated: 226; Mature: 225

Protein sequence:

>226_residues
MPRVCVFDVNETLLDLRALDPMFEQAFGDPGVRHEWFQQVIHLAMVVTITGSYVPFAQIGDAALTMTAARRGVKLSSSGR
QQILAGMRQLPPHPDVQPALERLQANGIRLATLTNSNVELAEAQLAFAGLRDFFEVRLSVEEVRRLKPAPEVYQMAAHRL
GVAMDQVRLIAAHAWDVAGALRAGCAAAFVARPGMVLDPLAPPPDVVGHDLAAVADQILVIERQAA

Sequences:

>Translated_226_residues
MPRVCVFDVNETLLDLRALDPMFEQAFGDPGVRHEWFQQVIHLAMVVTITGSYVPFAQIGDAALTMTAARRGVKLSSSGR
QQILAGMRQLPPHPDVQPALERLQANGIRLATLTNSNVELAEAQLAFAGLRDFFEVRLSVEEVRRLKPAPEVYQMAAHRL
GVAMDQVRLIAAHAWDVAGALRAGCAAAFVARPGMVLDPLAPPPDVVGHDLAAVADQILVIERQAA
>Mature_225_residues
PRVCVFDVNETLLDLRALDPMFEQAFGDPGVRHEWFQQVIHLAMVVTITGSYVPFAQIGDAALTMTAARRGVKLSSSGRQ
QILAGMRQLPPHPDVQPALERLQANGIRLATLTNSNVELAEAQLAFAGLRDFFEVRLSVEEVRRLKPAPEVYQMAAHRLG
VAMDQVRLIAAHAWDVAGALRAGCAAAFVARPGMVLDPLAPPPDVVGHDLAAVADQILVIERQAA

Specific function: Catalyzes the hydrolytic dehalogenation of small (S)-2- haloalkanoic acids to yield the corresponding (R)-2- hydroxyalkanoic acids. Acts on acids of short chain lengths, C(2) to C(4), with inversion of configuration at C-2 [H]

COG id: COG1011

COG function: function code R; Predicted hydrolase (HAD superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. S-2- haloalkanoic acid dehalogenase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006388
- InterPro:   IPR006328
- InterPro:   IPR005833 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: =3.8.1.2 [H]

Molecular weight: Translated: 24513; Mature: 24382

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRVCVFDVNETLLDLRALDPMFEQAFGDPGVRHEWFQQVIHLAMVVTITGSYVPFAQIG
CCCEEEEECCHHHHHHHHCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHC
DAALTMTAARRGVKLSSSGRQQILAGMRQLPPHPDVQPALERLQANGIRLATLTNSNVEL
CHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHCCCEEEEEEECCCCHH
AEAQLAFAGLRDFFEVRLSVEEVRRLKPAPEVYQMAAHRLGVAMDQVRLIAAHAWDVAGA
HHHHHHHHHHHHHHHHHHCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRAGCAAAFVARPGMVLDPLAPPPDVVGHDLAAVADQILVIERQAA
HHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHHHHEECCCC
>Mature Secondary Structure 
PRVCVFDVNETLLDLRALDPMFEQAFGDPGVRHEWFQQVIHLAMVVTITGSYVPFAQIG
CCEEEEECCHHHHHHHHCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHC
DAALTMTAARRGVKLSSSGRQQILAGMRQLPPHPDVQPALERLQANGIRLATLTNSNVEL
CHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHCCCEEEEEEECCCCHH
AEAQLAFAGLRDFFEVRLSVEEVRRLKPAPEVYQMAAHRLGVAMDQVRLIAAHAWDVAGA
HHHHHHHHHHHHHHHHHHCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRAGCAAAFVARPGMVLDPLAPPPDVVGHDLAAVADQILVIERQAA
HHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHHHHEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7764511 [H]