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The map label for this gene is lutR [H]

Identifier: 222526337

GI number: 222526337

Start: 3831698

End: 3832402

Strand: Reverse

Name: lutR [H]

Synonym: Chy400_3103

Alternate gene names: 222526337

Gene position: 3832402-3831698 (Counterclockwise)

Preceding gene: 222526338

Following gene: 222526336

Centisome position: 72.74

GC content: 60.71

Gene sequence:

>705_bases
ATGTCAATTACCAGACAACCGCTCGTTGAACAAGTAATCGAACATCTCCGACAGCAGGTTCAGGCACAGGTGTATCCGGT
AGGCAGCCGCTTACCACCAGAGCCACAACTGATGGCACAACTCGGCGTTGGGCGTTCGACGGTGCGCGAAGCGATTCGGG
CCTTGGCCCACGAAGGGTTGCTGGAAGTTCGCCAGGGCGATGGCACCTACGTGCGTGCAGCGGTCACTACAGAACCGTTA
GCAGCACGCCTGCGCCAGGCCAAAGTGCAGGAGGTTCACGAGGTTCGGCGGGCGCTCGAACTGGAGGTCGTTGCCCTGGC
CGCCGAGCGGCGCAGTGCCGATGATCTGACAATGATCCGGCACTGGCTGGCAGTGCGTACCACCGCGCTGGCAAACGGTG
ATACCACTGCGGCTCTCAATGCCGACATTGAATTGCACTGCGCTATTGCCGGTGCTACGCAGAATGCAATGCTGCGTGAT
CTGTATCGGATATTTGCCGTTACCTTGCGCGATGCGCTGGGTGTGCTGTGGGATGTCACAGCGCCGGCCCGCGATGACCA
TTCCCACGCCGATCTGGTGGCGGCCATCGAAGCCGGCGATGCTGAAACTGCCATTGCGGTGACCCGCCAGATTCTGGCTC
GCCACGAAGCGACAATTTATCGCTACGGCGCAGAGGAAGCGCACAGGCAGGAGGCTGAACGGTAG

Upstream 100 bases:

>100_bases
ACGTGGGAACAACCGGTGCAAGTTTCACCACGATTGACAGCCTGCTACACATCACGTATACTGAAACATCAGATGATATG
ATCTTTAGTCAGGAGTGATC

Downstream 100 bases:

>100_bases
AACAAGCAGGGAAAGGCGGTATGGCGCTGAACATCACTCACCAACCGACAGGCATGTACTCCCTGCTGACGGCAGCACCG
GCAGGTACAGGCAGACCGCA

Product: regulatory protein GntR HTH

Products: NA

Alternate protein names: L-lactate utilization operon repressor [H]

Number of amino acids: Translated: 234; Mature: 233

Protein sequence:

>234_residues
MSITRQPLVEQVIEHLRQQVQAQVYPVGSRLPPEPQLMAQLGVGRSTVREAIRALAHEGLLEVRQGDGTYVRAAVTTEPL
AARLRQAKVQEVHEVRRALELEVVALAAERRSADDLTMIRHWLAVRTTALANGDTTAALNADIELHCAIAGATQNAMLRD
LYRIFAVTLRDALGVLWDVTAPARDDHSHADLVAAIEAGDAETAIAVTRQILARHEATIYRYGAEEAHRQEAER

Sequences:

>Translated_234_residues
MSITRQPLVEQVIEHLRQQVQAQVYPVGSRLPPEPQLMAQLGVGRSTVREAIRALAHEGLLEVRQGDGTYVRAAVTTEPL
AARLRQAKVQEVHEVRRALELEVVALAAERRSADDLTMIRHWLAVRTTALANGDTTAALNADIELHCAIAGATQNAMLRD
LYRIFAVTLRDALGVLWDVTAPARDDHSHADLVAAIEAGDAETAIAVTRQILARHEATIYRYGAEEAHRQEAER
>Mature_233_residues
SITRQPLVEQVIEHLRQQVQAQVYPVGSRLPPEPQLMAQLGVGRSTVREAIRALAHEGLLEVRQGDGTYVRAAVTTEPLA
ARLRQAKVQEVHEVRRALELEVVALAAERRSADDLTMIRHWLAVRTTALANGDTTAALNADIELHCAIAGATQNAMLRDL
YRIFAVTLRDALGVLWDVTAPARDDHSHADLVAAIEAGDAETAIAVTRQILARHEATIYRYGAEEAHRQEAER

Specific function: Negatively regulates the transcription of the lutABC operon, which is required for L-lactate utilization. LutR activity is regulated by lactate, since presence of L-lactate, that probably binds to lutR, leads to derepression of the operon. Also appears to

COG id: COG2186

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790780, Length=143, Percent_Identity=35.6643356643357, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1786303, Length=233, Percent_Identity=31.7596566523605, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1790032, Length=235, Percent_Identity=27.2340425531915, Blast_Score=65, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011711
- InterPro:   IPR000524
- InterPro:   IPR011991 [H]

Pfam domain/function: PF07729 FCD; PF00392 GntR [H]

EC number: NA

Molecular weight: Translated: 25779; Mature: 25647

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSITRQPLVEQVIEHLRQQVQAQVYPVGSRLPPEPQLMAQLGVGRSTVREAIRALAHEGL
CCCCHHHHHHHHHHHHHHHHHHHHEECCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHCH
LEVRQGDGTYVRAAVTTEPLAARLRQAKVQEVHEVRRALELEVVALAAERRSADDLTMIR
HHEECCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
HWLAVRTTALANGDTTAALNADIELHCAIAGATQNAMLRDLYRIFAVTLRDALGVLWDVT
HHHHHHHHHHCCCCCCEEEECCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
APARDDHSHADLVAAIEAGDAETAIAVTRQILARHEATIYRYGAEEAHRQEAER
CCCCCCCCHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHEECCCHHHHHHHCCC
>Mature Secondary Structure 
SITRQPLVEQVIEHLRQQVQAQVYPVGSRLPPEPQLMAQLGVGRSTVREAIRALAHEGL
CCCHHHHHHHHHHHHHHHHHHHHEECCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHCH
LEVRQGDGTYVRAAVTTEPLAARLRQAKVQEVHEVRRALELEVVALAAERRSADDLTMIR
HHEECCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
HWLAVRTTALANGDTTAALNADIELHCAIAGATQNAMLRDLYRIFAVTLRDALGVLWDVT
HHHHHHHHHHCCCCCCEEEECCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
APARDDHSHADLVAAIEAGDAETAIAVTRQILARHEATIYRYGAEEAHRQEAER
CCCCCCCCHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHEECCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]