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The map label for this gene is regX3 [H]

Identifier: 222526334

GI number: 222526334

Start: 3825001

End: 3825699

Strand: Direct

Name: regX3 [H]

Synonym: Chy400_3100

Alternate gene names: 222526334

Gene position: 3825001-3825699 (Clockwise)

Preceding gene: 222526333

Following gene: 222526335

Centisome position: 72.6

GC content: 58.08

Gene sequence:

>699_bases
ATGGCGACGGTTCTCATCGTCGAAGACGAAACCACGTTGGCCGAGACACTACGCTACAACCTCGAACGCGAAGGCTATTC
CGTCATTGTCGCCGGTGATGGTGTTCAGGGTCTCGACCGTGCTCGCCGTGATCAACCCGATCTCGTTGTCCTCGACATTA
TGCTCCCCCGTCTTGACGGCTTCTCGGTCTGTCGGATTCTGCGTCAAGAGAGCGATGTACCGATCATCATGCTCACGGCT
CGTCAGGACGAGGTTGATCGGATTGCCGGTCTGGAACTGGGGGCTGATGACTACATGGGCAAGCCGTTTAGCCTCGGCGA
GTTCCTTGCTCGTGTGCGGGCGATCCTGCGGCGCAGCGAACGACAGCCTCATTCGATTGTACGTGAAGTGCTAGAGGCCG
GGGCGATCCGGGTGGACACCAGCAGCCGTCGGGCCTGGCGGAATGGGCAGGAACTGAATCTCCCGCAGAAAGAATTCGAT
CTGCTCACCTGTCTGATGCGTAATCGTGGGATTGCCCTGACGCGCGATCTGCTGCTCGAACGGGTATGGGGACAGGACTT
CATCGGTGATAGTCGCACTGTCGATGTCCATATCCGCTGGCTGCGCGAGAAGATAGAGCCAGACCCTGGCAAACCGGTTT
ATATCCAGACGGTGCGAGGGGTTGGGTATCGGTTTGAACCGCCATCTGATGATGCGTAA

Upstream 100 bases:

>100_bases
ACGCGGGGGTCGTGGGTTCAAATCCCTCCGCCCCGACCACATTCGCCACGTGGCAATGACCGCCGACTCACAAGCGGCGG
TCTTTATGTGTGGAGTAACT

Downstream 100 bases:

>100_bases
ATTGCACCAATGATGCTGCTCGAATGGATGCTGATGGTAGCGACGATTGCACTGGCGGTTGGGTTTATCGTCAGTATGTA
TCGCCGACATCAGAAGAGCC

Product: winged helix family two component transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 232; Mature: 231

Protein sequence:

>232_residues
MATVLIVEDETTLAETLRYNLEREGYSVIVAGDGVQGLDRARRDQPDLVVLDIMLPRLDGFSVCRILRQESDVPIIMLTA
RQDEVDRIAGLELGADDYMGKPFSLGEFLARVRAILRRSERQPHSIVREVLEAGAIRVDTSSRRAWRNGQELNLPQKEFD
LLTCLMRNRGIALTRDLLLERVWGQDFIGDSRTVDVHIRWLREKIEPDPGKPVYIQTVRGVGYRFEPPSDDA

Sequences:

>Translated_232_residues
MATVLIVEDETTLAETLRYNLEREGYSVIVAGDGVQGLDRARRDQPDLVVLDIMLPRLDGFSVCRILRQESDVPIIMLTA
RQDEVDRIAGLELGADDYMGKPFSLGEFLARVRAILRRSERQPHSIVREVLEAGAIRVDTSSRRAWRNGQELNLPQKEFD
LLTCLMRNRGIALTRDLLLERVWGQDFIGDSRTVDVHIRWLREKIEPDPGKPVYIQTVRGVGYRFEPPSDDA
>Mature_231_residues
ATVLIVEDETTLAETLRYNLEREGYSVIVAGDGVQGLDRARRDQPDLVVLDIMLPRLDGFSVCRILRQESDVPIIMLTAR
QDEVDRIAGLELGADDYMGKPFSLGEFLARVRAILRRSERQPHSIVREVLEAGAIRVDTSSRRAWRNGQELNLPQKEFDL
LTCLMRNRGIALTRDLLLERVWGQDFIGDSRTVDVHIRWLREKIEPDPGKPVYIQTVRGVGYRFEPPSDDA

Specific function: Part of the two-component regulatory system senX3/regX3. Once phosphorylated by senX3, activates the expression of several operons/genes involved in phosphate assimilation and metabolism, such as pstSCAB, phnDCE, and phoA [H]

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786784, Length=226, Percent_Identity=42.0353982300885, Blast_Score=167, Evalue=8e-43,
Organism=Escherichia coli, GI1786911, Length=227, Percent_Identity=41.8502202643172, Blast_Score=162, Evalue=2e-41,
Organism=Escherichia coli, GI1786599, Length=225, Percent_Identity=41.3333333333333, Blast_Score=161, Evalue=3e-41,
Organism=Escherichia coli, GI1789809, Length=234, Percent_Identity=40.5982905982906, Blast_Score=157, Evalue=6e-40,
Organism=Escherichia coli, GI87082012, Length=222, Percent_Identity=40.0900900900901, Blast_Score=156, Evalue=9e-40,
Organism=Escherichia coli, GI1788394, Length=223, Percent_Identity=40.3587443946188, Blast_Score=154, Evalue=3e-39,
Organism=Escherichia coli, GI1790860, Length=225, Percent_Identity=41.3333333333333, Blast_Score=150, Evalue=6e-38,
Organism=Escherichia coli, GI2367329, Length=229, Percent_Identity=41.9213973799127, Blast_Score=140, Evalue=5e-35,
Organism=Escherichia coli, GI1790863, Length=229, Percent_Identity=36.6812227074236, Blast_Score=134, Evalue=5e-33,
Organism=Escherichia coli, GI1789402, Length=221, Percent_Identity=37.10407239819, Blast_Score=133, Evalue=1e-32,
Organism=Escherichia coli, GI1790552, Length=224, Percent_Identity=40.1785714285714, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI1787229, Length=226, Percent_Identity=37.6106194690265, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1787375, Length=224, Percent_Identity=33.4821428571429, Blast_Score=117, Evalue=5e-28,
Organism=Escherichia coli, GI145693140, Length=235, Percent_Identity=32.7659574468085, Blast_Score=115, Evalue=2e-27,
Organism=Escherichia coli, GI1788713, Length=104, Percent_Identity=37.5, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1788550, Length=106, Percent_Identity=32.0754716981132, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1790299, Length=121, Percent_Identity=29.7520661157025, Blast_Score=61, Evalue=8e-11,
Organism=Saccharomyces cerevisiae, GI6322000, Length=102, Percent_Identity=33.3333333333333, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 26456; Mature: 26324

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATVLIVEDETTLAETLRYNLEREGYSVIVAGDGVQGLDRARRDQPDLVVLDIMLPRLDG
CEEEEEEECCHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHCCCCCEEEEEEECCCCCH
FSVCRILRQESDVPIIMLTARQDEVDRIAGLELGADDYMGKPFSLGEFLARVRAILRRSE
HHHHHHHHHCCCCCEEEEECCCCHHHHHHCCEECCHHHCCCCCCHHHHHHHHHHHHHHCC
RQPHSIVREVLEAGAIRVDTSSRRAWRNGQELNLPQKEFDLLTCLMRNRGIALTRDLLLE
CCHHHHHHHHHHCCCEEEECCCHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHH
RVWGQDFIGDSRTVDVHIRWLREKIEPDPGKPVYIQTVRGVGYRFEPPSDDA
HHHCCCCCCCCCEEEEHHHHHHHHCCCCCCCCEEEEEECCCCEECCCCCCCC
>Mature Secondary Structure 
ATVLIVEDETTLAETLRYNLEREGYSVIVAGDGVQGLDRARRDQPDLVVLDIMLPRLDG
EEEEEEECCHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHCCCCCEEEEEEECCCCCH
FSVCRILRQESDVPIIMLTARQDEVDRIAGLELGADDYMGKPFSLGEFLARVRAILRRSE
HHHHHHHHHCCCCCEEEEECCCCHHHHHHCCEECCHHHCCCCCCHHHHHHHHHHHHHHCC
RQPHSIVREVLEAGAIRVDTSSRRAWRNGQELNLPQKEFDLLTCLMRNRGIALTRDLLLE
CCHHHHHHHHHHCCCEEEECCCHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHH
RVWGQDFIGDSRTVDVHIRWLREKIEPDPGKPVYIQTVRGVGYRFEPPSDDA
HHHCCCCCCCCCEEEEHHHHHHHHCCCCCCCCEEEEEECCCCEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA