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The map label for this gene is nspVM [H]

Identifier: 222526306

GI number: 222526306

Start: 3790387

End: 3791958

Strand: Reverse

Name: nspVM [H]

Synonym: Chy400_3072

Alternate gene names: 222526306

Gene position: 3791958-3790387 (Counterclockwise)

Preceding gene: 222526312

Following gene: 222526304

Centisome position: 71.97

GC content: 50.06

Gene sequence:

>1572_bases
ATGAACCGACAACTTCGCAACGTTGAATTCGGAGATTTTCAAACACCTGTTAGCCTGGCAAGAGATATTTGTGCGCTGAT
TGCGCGAACTGGCTTTCAGCCTGCTGCCATTCTCGAACCCACCTGTGGGACGGGGGCATTCCTTCAAGCAGCATTGGAAA
CATTTCCAGGTGTAACGCGCATTCTTGGGTTTGATATCAATCCACAACATATCACGCAGGCACGACACACCATCACACCC
ATCAACCCTCATTCACCTGCATCTGTTGAAATATGTCAGGCCGACTTCTTTCGTACCGACTGGACAAATATTGTTGCAGC
ACTACCAGAACCACTCCTGGTGATTGGCAACCCACCGTGGGTCACAAACACAGCACTAAGCACGCTTAGCAGTACGAATC
TTCCCGTAAAGTCAAACCGCGACCATCTTCGTGGCATTGACGCGCTTACCGGAAGTAGTAACTTCGACATTTCAGAATGG
ATAATGAGAGAGATCATTTCCTGGTTTAGAGATAAAACCGGACTGCTGGCAATGCTCTGCAAGACAACCGTTGCCCGCAA
GGTGCTCAAATACGCCTGGCACAACGGAATACACTCCGGCACAGCATCACTCTATCTTATCGACGCGCACACACATTTCG
GTGCTGCGGTCGACGCCTGTTTACTACTTGTACCGATTACTCCAACCGGTACAGACAAGGTGTGTCAGGTGTTTCATTCT
CTGGATGCAGTGCAACCTGCCAGCACCTTTGGATTACGAGAAGGCATACTGGTGGCTGATACTGCATTACCTCCGCATTG
GCAAGAGCTGGGAGGAAATAGCCTGAAGGTCTGGCGGTCAGGAATAAAGCACGATTGCAGTAACGTCTTTGAACTGCACG
TTGAAGGTGACAGGCTCGTGAACGGACTGGGAACACAGGTTGATATTGAACCTGAAGTACTTTTCCCACTCTTGAAGAGC
GCTGACCTGGCAGCCCGACGCCGACCACAACGCTGGATTCTGGTTCCACAGCGAGCAGCAAATGATGATCCGAACCATCT
TAAGACCCATGCGCCTAAAGCGTGGCATTACCTGACTGCTCACGCCCATCTGTTAAACAAAAGGAAGAGCCAGATATACA
AAAACCGCCCACGATTTTCTATCTTTGATGTTGGATCGTATTCGTTCGCTCCATGGAAGGTTGCAATCTCAGGTCTCTAC
AAAAAACTCGAATTCGTACAGGTACCACCGTATGCAGATCGCCCGGTCATGCTTGATGATACCTGCTACTTCTTTTCCTG
TTATTCCGCAGAAGCCTGCAACCTGCTCTACGAGCTGGTCACATCCGAACCGGCCAAAGCATTCTGGTCATCCAGGATTT
TCTGGGATGCCAAGCGACCAATTACGGCACATTTGCTGAATGCACTCGATCTCAGGCGTCTTGCTCGATTGTTGGGAAAA
GAAGCTGCTGTTGACCAACTGCTCGCGGAACGACAAATTGCTGAGTCTACCCAGGGCGCAAACCAGTATCTTCTGTTTCA
AAACGGCTTCGCTGAAGAGGAGACGGAGGTACCCGGAAGAGAAGAACGCTGA

Upstream 100 bases:

>100_bases
GCAATCTCACCATCTCGAATGCACTTCAGTGGCGCCTGCAATACAGTCGAGTTATCGAGCAAGCTGGTTTCGTTGCAGGA
ATTGAAAGACTGAGATAGAC

Downstream 100 bases:

>100_bases
GCAATGCTATGATCACCACAAAGACACGACGGACACAGAGAATGCTCAAAACAGTCTACTACCCTCTGTGCTCTCAGTGC
CTCTCTGGTGCAACACCGCC

Product: modification methylase NspV

Products: NA

Alternate protein names: M.NspV; Methyltransferase NspV [H]

Number of amino acids: Translated: 523; Mature: 523

Protein sequence:

>523_residues
MNRQLRNVEFGDFQTPVSLARDICALIARTGFQPAAILEPTCGTGAFLQAALETFPGVTRILGFDINPQHITQARHTITP
INPHSPASVEICQADFFRTDWTNIVAALPEPLLVIGNPPWVTNTALSTLSSTNLPVKSNRDHLRGIDALTGSSNFDISEW
IMREIISWFRDKTGLLAMLCKTTVARKVLKYAWHNGIHSGTASLYLIDAHTHFGAAVDACLLLVPITPTGTDKVCQVFHS
LDAVQPASTFGLREGILVADTALPPHWQELGGNSLKVWRSGIKHDCSNVFELHVEGDRLVNGLGTQVDIEPEVLFPLLKS
ADLAARRRPQRWILVPQRAANDDPNHLKTHAPKAWHYLTAHAHLLNKRKSQIYKNRPRFSIFDVGSYSFAPWKVAISGLY
KKLEFVQVPPYADRPVMLDDTCYFFSCYSAEACNLLYELVTSEPAKAFWSSRIFWDAKRPITAHLLNALDLRRLARLLGK
EAAVDQLLAERQIAESTQGANQYLLFQNGFAEEETEVPGREER

Sequences:

>Translated_523_residues
MNRQLRNVEFGDFQTPVSLARDICALIARTGFQPAAILEPTCGTGAFLQAALETFPGVTRILGFDINPQHITQARHTITP
INPHSPASVEICQADFFRTDWTNIVAALPEPLLVIGNPPWVTNTALSTLSSTNLPVKSNRDHLRGIDALTGSSNFDISEW
IMREIISWFRDKTGLLAMLCKTTVARKVLKYAWHNGIHSGTASLYLIDAHTHFGAAVDACLLLVPITPTGTDKVCQVFHS
LDAVQPASTFGLREGILVADTALPPHWQELGGNSLKVWRSGIKHDCSNVFELHVEGDRLVNGLGTQVDIEPEVLFPLLKS
ADLAARRRPQRWILVPQRAANDDPNHLKTHAPKAWHYLTAHAHLLNKRKSQIYKNRPRFSIFDVGSYSFAPWKVAISGLY
KKLEFVQVPPYADRPVMLDDTCYFFSCYSAEACNLLYELVTSEPAKAFWSSRIFWDAKRPITAHLLNALDLRRLARLLGK
EAAVDQLLAERQIAESTQGANQYLLFQNGFAEEETEVPGREER
>Mature_523_residues
MNRQLRNVEFGDFQTPVSLARDICALIARTGFQPAAILEPTCGTGAFLQAALETFPGVTRILGFDINPQHITQARHTITP
INPHSPASVEICQADFFRTDWTNIVAALPEPLLVIGNPPWVTNTALSTLSSTNLPVKSNRDHLRGIDALTGSSNFDISEW
IMREIISWFRDKTGLLAMLCKTTVARKVLKYAWHNGIHSGTASLYLIDAHTHFGAAVDACLLLVPITPTGTDKVCQVFHS
LDAVQPASTFGLREGILVADTALPPHWQELGGNSLKVWRSGIKHDCSNVFELHVEGDRLVNGLGTQVDIEPEVLFPLLKS
ADLAARRRPQRWILVPQRAANDDPNHLKTHAPKAWHYLTAHAHLLNKRKSQIYKNRPRFSIFDVGSYSFAPWKVAISGLY
KKLEFVQVPPYADRPVMLDDTCYFFSCYSAEACNLLYELVTSEPAKAFWSSRIFWDAKRPITAHLLNALDLRRLARLLGK
EAAVDQLLAERQIAESTQGANQYLLFQNGFAEEETEVPGREER

Specific function: This methylase recognizes the double-stranded sequence TTCGAA, and methylates it on a yet unknown base to protect it against the NspV endonuclease [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 58404; Mature: 58404

Theoretical pI: Translated: 7.43; Mature: 7.43

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRQLRNVEFGDFQTPVSLARDICALIARTGFQPAAILEPTCGTGAFLQAALETFPGVTR
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHHCCCHHH
ILGFDINPQHITQARHTITPINPHSPASVEICQADFFRTDWTNIVAALPEPLLVIGNPPW
HHCCCCCHHHHHHHHHCCCCCCCCCCCCEEEEHHHHHHCCHHHHHHHCCCCEEEECCCCC
VTNTALSTLSSTNLPVKSNRDHLRGIDALTGSSNFDISEWIMREIISWFRDKTGLLAMLC
CCHHHHHHHHCCCCCCCCCCHHHCCCHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHH
KTTVARKVLKYAWHNGIHSGTASLYLIDAHTHFGAAVDACLLLVPITPTGTDKVCQVFHS
HHHHHHHHHHHHHHCCCCCCCEEEEEEECCCHHHHHHHEEEEEEECCCCCHHHHHHHHHH
LDAVQPASTFGLREGILVADTALPPHWQELGGNSLKVWRSGIKHDCSNVFELHVEGDRLV
HCCCCCHHHHHCCCCEEEEECCCCCCHHHHCCCHHHHHHHHHHHHCCCCEEEEECHHHHH
NGLGTQVDIEPEVLFPLLKSADLAARRRPQRWILVPQRAANDDPNHLKTHAPKAWHYLTA
HCCCCEEECCHHHHHHHHHCCCHHHHCCCCEEEEECCCCCCCCCCHHHHCCCCHHHHHHH
HAHLLNKRKSQIYKNRPRFSIFDVGSYSFAPWKVAISGLYKKLEFVQVPPYADRPVMLDD
HHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHEEECCCCCCCCEEEEC
TCYFFSCYSAEACNLLYELVTSEPAKAFWSSRIFWDAKRPITAHLLNALDLRRLARLLGK
CEEEEEECCHHHHHHHHHHHHCCHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHHHHHCC
EAAVDQLLAERQIAESTQGANQYLLFQNGFAEEETEVPGREER
HHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNRQLRNVEFGDFQTPVSLARDICALIARTGFQPAAILEPTCGTGAFLQAALETFPGVTR
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHHCCCHHH
ILGFDINPQHITQARHTITPINPHSPASVEICQADFFRTDWTNIVAALPEPLLVIGNPPW
HHCCCCCHHHHHHHHHCCCCCCCCCCCCEEEEHHHHHHCCHHHHHHHCCCCEEEECCCCC
VTNTALSTLSSTNLPVKSNRDHLRGIDALTGSSNFDISEWIMREIISWFRDKTGLLAMLC
CCHHHHHHHHCCCCCCCCCCHHHCCCHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHH
KTTVARKVLKYAWHNGIHSGTASLYLIDAHTHFGAAVDACLLLVPITPTGTDKVCQVFHS
HHHHHHHHHHHHHHCCCCCCCEEEEEEECCCHHHHHHHEEEEEEECCCCCHHHHHHHHHH
LDAVQPASTFGLREGILVADTALPPHWQELGGNSLKVWRSGIKHDCSNVFELHVEGDRLV
HCCCCCHHHHHCCCCEEEEECCCCCCHHHHCCCHHHHHHHHHHHHCCCCEEEEECHHHHH
NGLGTQVDIEPEVLFPLLKSADLAARRRPQRWILVPQRAANDDPNHLKTHAPKAWHYLTA
HCCCCEEECCHHHHHHHHHCCCHHHHCCCCEEEEECCCCCCCCCCHHHHCCCCHHHHHHH
HAHLLNKRKSQIYKNRPRFSIFDVGSYSFAPWKVAISGLYKKLEFVQVPPYADRPVMLDD
HHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHEEECCCCCCCCEEEEC
TCYFFSCYSAEACNLLYELVTSEPAKAFWSSRIFWDAKRPITAHLLNALDLRRLARLLGK
CEEEEEECCHHHHHHHHHHHHCCHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHHHHHCC
EAAVDQLLAERQIAESTQGANQYLLFQNGFAEEETEVPGREER
HHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8367311 [H]