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The map label for this gene is lipB

Identifier: 222526305

GI number: 222526305

Start: 3789608

End: 3790279

Strand: Direct

Name: lipB

Synonym: Chy400_3071

Alternate gene names: 222526305

Gene position: 3789608-3790279 (Clockwise)

Preceding gene: 222526298

Following gene: 222526307

Centisome position: 71.92

GC content: 61.01

Gene sequence:

>672_bases
ATGCGCACGTTGACGGTTCATTATCTGGGGCAAATAGCCTATACCGCCGCCTGGGACATTCAGCGTCAACTGGCTGCCGA
GCGGTCGCGTGGGCAGATTGGCGATACGCTCCTCTTGCTGGAGCACCCGCCGACGATCACCCTGGGGAACAAAGCCCGAC
CCGATCATGTGTTGGCGTCACCTGCCGAACTGGCAGCGCGTGGCGTTGCCGTGGTGCAGAGTGATCGGGGTGGCGAGGTG
ACCTATCATGCGCCGGGTCAACTGGTTGCCTATCCGATCTTCAAACTCTCGCAGCATGGGAGCGATGTGGGCCGCTACGT
GCGCGGTTTGGAAGAGAGTGTTATTCGCGTGTTAGCCGGTTATGGTCTGGTGGGTGAGCGGGTTGCCGGATTGACCGGGG
TCTGGGTGCGCAACGGTGCGGCGAAGATTTGTGCCATTGGCGTGAAGCTCAGCGCGAGTGGGGTGACAACCCATGGCCTG
GCGCTCAACGTCGATCCCGATCTCAGCGGGTTTGATTTGATTGTACCCTGTGGTATCACTGATCGCAGCGTGACCTCGCT
GGCGTTTGAACTGGGGCAAGCGCCAGCGCTGGCCGAGGTAGCCGAGCGCCTGATCGCACAGATAGGCGAGGTGTTTGCCC
TTGAACCGCGGGTTGAAGCGCTGGCGATGTAA

Upstream 100 bases:

>100_bases
TGGTGCCCACAGGCAATCTTCCCGGCAGGGCGACGGGATCGGTTATACTGAAAAAGATGCCGTTAGCAACACCCGGAGGA
TCGCAGGAACGAGGCTCACC

Downstream 100 bases:

>100_bases
AGGTTTGGGCGGTGTTGCACCAGAGAGGCACTGAGAGCACAGAGGGTAGTAGACTGTTTTGAGCATTCTCTGTGTCCGTC
GTGTCTTTGTGGTGATCATA

Product: lipoate-protein ligase B

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase

Number of amino acids: Translated: 223; Mature: 223

Protein sequence:

>223_residues
MRTLTVHYLGQIAYTAAWDIQRQLAAERSRGQIGDTLLLLEHPPTITLGNKARPDHVLASPAELAARGVAVVQSDRGGEV
TYHAPGQLVAYPIFKLSQHGSDVGRYVRGLEESVIRVLAGYGLVGERVAGLTGVWVRNGAAKICAIGVKLSASGVTTHGL
ALNVDPDLSGFDLIVPCGITDRSVTSLAFELGQAPALAEVAERLIAQIGEVFALEPRVEALAM

Sequences:

>Translated_223_residues
MRTLTVHYLGQIAYTAAWDIQRQLAAERSRGQIGDTLLLLEHPPTITLGNKARPDHVLASPAELAARGVAVVQSDRGGEV
TYHAPGQLVAYPIFKLSQHGSDVGRYVRGLEESVIRVLAGYGLVGERVAGLTGVWVRNGAAKICAIGVKLSASGVTTHGL
ALNVDPDLSGFDLIVPCGITDRSVTSLAFELGQAPALAEVAERLIAQIGEVFALEPRVEALAM
>Mature_223_residues
MRTLTVHYLGQIAYTAAWDIQRQLAAERSRGQIGDTLLLLEHPPTITLGNKARPDHVLASPAELAARGVAVVQSDRGGEV
TYHAPGQLVAYPIFKLSQHGSDVGRYVRGLEESVIRVLAGYGLVGERVAGLTGVWVRNGAAKICAIGVKLSASGVTTHGL
ALNVDPDLSGFDLIVPCGITDRSVTSLAFELGQAPALAEVAERLIAQIGEVFALEPRVEALAM

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG0321

COG function: function code H; Lipoate-protein ligase B

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipB family

Homologues:

Organism=Homo sapiens, GI221554520, Length=153, Percent_Identity=38.562091503268, Blast_Score=91, Evalue=9e-19,
Organism=Escherichia coli, GI87081767, Length=174, Percent_Identity=37.3563218390805, Blast_Score=102, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6323268, Length=198, Percent_Identity=30.8080808080808, Blast_Score=85, Evalue=9e-18,
Organism=Drosophila melanogaster, GI21355483, Length=213, Percent_Identity=37.0892018779343, Blast_Score=124, Evalue=6e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPB_CHLAA (A9WKF8)

Other databases:

- EMBL:   CP000909
- RefSeq:   YP_001636425.1
- ProteinModelPortal:   A9WKF8
- SMR:   A9WKF8
- GeneID:   5827306
- GenomeReviews:   CP000909_GR
- KEGG:   cau:Caur_2835
- HOGENOM:   HBG716160
- OMA:   HIEMSEF
- ProtClustDB:   CLSK973306
- GO:   GO:0005737
- HAMAP:   MF_00013
- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605
- PIRSF:   PIRSF016262
- TIGRFAMs:   TIGR00214

Pfam domain/function: PF03099 BPL_LipA_LipB

EC number: =2.3.1.181

Molecular weight: Translated: 23581; Mature: 23581

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS01313 LIPB

Important sites: ACT_SITE 177-177

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTLTVHYLGQIAYTAAWDIQRQLAAERSRGQIGDTLLLLEHPPTITLGNKARPDHVLAS
CCEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCEEEECCCCCCCCCCCC
PAELAARGVAVVQSDRGGEVTYHAPGQLVAYPIFKLSQHGSDVGRYVRGLEESVIRVLAG
HHHHHHCCEEEEECCCCCEEEEECCCCEEEHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
YGLVGERVAGLTGVWVRNGAAKICAIGVKLSASGVTTHGLALNVDPDLSGFDLIVPCGIT
CCHHHHHHCCCEEEEEECCCCEEEEEEEEEECCCCEECCEEEEECCCCCCCEEEEECCCC
DRSVTSLAFELGQAPALAEVAERLIAQIGEVFALEPRVEALAM
CHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHEECCCCHHHHCC
>Mature Secondary Structure
MRTLTVHYLGQIAYTAAWDIQRQLAAERSRGQIGDTLLLLEHPPTITLGNKARPDHVLAS
CCEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCEEEECCCCCCCCCCCC
PAELAARGVAVVQSDRGGEVTYHAPGQLVAYPIFKLSQHGSDVGRYVRGLEESVIRVLAG
HHHHHHCCEEEEECCCCCEEEEECCCCEEEHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
YGLVGERVAGLTGVWVRNGAAKICAIGVKLSASGVTTHGLALNVDPDLSGFDLIVPCGIT
CCHHHHHHCCCEEEEEECCCCEEEEEEEEEECCCCEECCEEEEECCCCCCCEEEEECCCC
DRSVTSLAFELGQAPALAEVAERLIAQIGEVFALEPRVEALAM
CHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHEECCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA