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The map label for this gene is puuR [H]

Identifier: 222526115

GI number: 222526115

Start: 3557847

End: 3558392

Strand: Direct

Name: puuR [H]

Synonym: Chy400_2872

Alternate gene names: 222526115

Gene position: 3557847-3558392 (Clockwise)

Preceding gene: 222526114

Following gene: 222526116

Centisome position: 67.52

GC content: 55.31

Gene sequence:

>546_bases
ATGTCACTCGGACAAAAGATCGGTCGTCTGCGTCAAGAACGTGGTCTGACCCTCCAGGAGGTGTCAGAGGGATCGGGGCT
AACTCCCTCGTTCCTGAGCCGTCTTGAACGTGATAAGGTTAATATTTCGGTGGCCAACCTGCGCAAACTGGCACAGTTTT
TTAGTGTCCAGATGACGCATTTTTTCGAGGGAGAGGACGATCAACAGCTTGGCCAGGTAGTGCGAGTCGCTGACCGTGTT
CGCCTCTCGCTCGATGATGCGCCGGTGCAGGTCTTTTCGTTGCTGCCACCCAACAGCGATCTCGATGCGCGCCTGATCGA
AGCCTATCCTGGCAGCAGTCAGCAGGGGTTCTCATCACGTGGCAGTCAGATGGTGCATGTCCTGGCTGGTCATGTGCGGT
ATACTTTGGGTGAGGAGCAGTACGATCTCGCCCCCGGCGATACGCTCTTCTTTCGCGATGATACGGCGTATAGCTGGACG
AATACCGGCCATTCGATTGCGACTATGCTAACGGTCAGTACTCTCAATCCACGTGATGAGCGCTGA

Upstream 100 bases:

>100_bases
TATAATCACATCTGACAATATGCAACAGTTGAGATAGTGGTATGTTCGGCGGCACAGACAAAAGTGCCGGCTATCGTTTT
TCTTTATATAGAGCTAACCT

Downstream 100 bases:

>100_bases
CACGTTTTGGTGAGGTTTCATGAGTATTCTGACCCCTGCGTTCCTCCGTCAGGCTGCCCGCTGGCTGCTGATTGGTTTTT
CAATTTACCTCATCGGCTGG

Product: XRE family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 181; Mature: 180

Protein sequence:

>181_residues
MSLGQKIGRLRQERGLTLQEVSEGSGLTPSFLSRLERDKVNISVANLRKLAQFFSVQMTHFFEGEDDQQLGQVVRVADRV
RLSLDDAPVQVFSLLPPNSDLDARLIEAYPGSSQQGFSSRGSQMVHVLAGHVRYTLGEEQYDLAPGDTLFFRDDTAYSWT
NTGHSIATMLTVSTLNPRDER

Sequences:

>Translated_181_residues
MSLGQKIGRLRQERGLTLQEVSEGSGLTPSFLSRLERDKVNISVANLRKLAQFFSVQMTHFFEGEDDQQLGQVVRVADRV
RLSLDDAPVQVFSLLPPNSDLDARLIEAYPGSSQQGFSSRGSQMVHVLAGHVRYTLGEEQYDLAPGDTLFFRDDTAYSWT
NTGHSIATMLTVSTLNPRDER
>Mature_180_residues
SLGQKIGRLRQERGLTLQEVSEGSGLTPSFLSRLERDKVNISVANLRKLAQFFSVQMTHFFEGEDDQQLGQVVRVADRVR
LSLDDAPVQVFSLLPPNSDLDARLIEAYPGSSQQGFSSRGSQMVHVLAGHVRYTLGEEQYDLAPGDTLFFRDDTAYSWTN
TGHSIATMLTVSTLNPRDER

Specific function: Represses puuD [H]

COG id: COG1396

COG function: function code K; Predicted transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH cro/C1-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787557, Length=179, Percent_Identity=26.2569832402235, Blast_Score=71, Evalue=4e-14,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013096
- InterPro:   IPR011051
- InterPro:   IPR001387
- InterPro:   IPR010982
- InterPro:   IPR014710 [H]

Pfam domain/function: PF07883 Cupin_2; PF01381 HTH_3 [H]

EC number: NA

Molecular weight: Translated: 20205; Mature: 20074

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: PS50943 HTH_CROC1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLGQKIGRLRQERGLTLQEVSEGSGLTPSFLSRLERDKVNISVANLRKLAQFFSVQMTH
CCHHHHHHHHHHHHCCCHHHHCCCCCCCHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHH
FFEGEDDQQLGQVVRVADRVRLSLDDAPVQVFSLLPPNSDLDARLIEAYPGSSQQGFSSR
EECCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCEEECCCCCCCCCCHHH
GSQMVHVLAGHVRYTLGEEQYDLAPGDTLFFRDDTAYSWTNTGHSIATMLTVSTLNPRDE
HHHHHHHHHHHHHEECCCCCCCCCCCCEEEEECCCCCEECCCCCCEEEEEEEECCCCCCC
R
C
>Mature Secondary Structure 
SLGQKIGRLRQERGLTLQEVSEGSGLTPSFLSRLERDKVNISVANLRKLAQFFSVQMTH
CHHHHHHHHHHHHCCCHHHHCCCCCCCHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHH
FFEGEDDQQLGQVVRVADRVRLSLDDAPVQVFSLLPPNSDLDARLIEAYPGSSQQGFSSR
EECCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCEEECCCCCCCCCCHHH
GSQMVHVLAGHVRYTLGEEQYDLAPGDTLFFRDDTAYSWTNTGHSIATMLTVSTLNPRDE
HHHHHHHHHHHHHEECCCCCCCCCCCCEEEEECCCCCEECCCCCCEEEEEEEECCCCCCC
R
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1840553 [H]