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The map label for this gene is cobD [H]

Identifier: 222526026

GI number: 222526026

Start: 3426568

End: 3427530

Strand: Reverse

Name: cobD [H]

Synonym: Chy400_2782

Alternate gene names: 222526026

Gene position: 3427530-3426568 (Counterclockwise)

Preceding gene: 222526027

Following gene: 222526025

Centisome position: 65.05

GC content: 63.45

Gene sequence:

>963_bases
ATGATTGGTGAAGAGCTACGTCGGCGTTGCGCGATTGCCCTCCTGGCCCTGGTGCTCGACTTCGCGTTGGGTGATCCGCC
CAACCAGCTTCATCCGGTGAGCCTGATGGGGCATTGGTTGAAATGGGGGGAACGATTAGCACCAGCGACACCGTTGGCCC
GCCTGGCCTGGGGTGGTGGGTGGCTATACGGGGGCTGGTGGCTGGTGGGGAGAGTGGCTTCCCGCCTGCCGCGTCATCTG
CTGGTGCAGGGGGCATGCGTTTCCCTGCTTCTGGCCTACCGGGGACTTGATCGAGCAGTTGCCGGGGTGGAACAGGCGTT
GCGAACGGGCAATCTTGCAGAAGCGCGTCATCTGCTTGGCTGGCACCTCGTCAGTCGGCCAACCGCCGACCTTAGTGCGG
CTGAAGTGGCCGGTGCAGCCATCGAGTCGCTGGCCGAAAATCTGAGTGACAGCATCATCGCTCCTCTGTTGGCCTTTCTG
GTGGGTGGTCTGCCCGCCCTGGCTATCTACCGCCTGACCAACACCGCCGATGCGATGTGGGGGTATCGCACAGAGCGCTA
CGAGTATCTGGGCAAAATTGCTGCCCGCTGCGACGATGCCTTCAACCTGGTACCGGCCCGCCTGACCGCACTGCTACTTG
CGCTGGCTACCCACCTGATCAACGGACGGGGGTGGCACGCCTGGCAGACCGCCCTGCGTGACGCCCGGCACACTGCTTCG
CCGAATGCCGGCTGGCCGATGGCTGCTATGGCCGGTGCGCTGGATACCGTCCTCACCAAACGCGATCACTACGCCCTGGG
TGATGGGAGCTGTATTCCTGATGCGGCGATGATTGGGCAAGCGCGCCGTATCGTGCAGGTAGCAATGGCGATAGTGGTGA
GTGGACTGTGGATCGGCATACTTACCGGTGCAGGGATACGGCGTGCCGCGCCTCTGCACCCAGATGCCACGCTAAGGGGG
TGA

Upstream 100 bases:

>100_bases
GTTTTGGATGCGGGTAGCGTGGCAGCCGTGGGAACGCCGGCGCGTGTGTTTGCTGATGAGACCCTGCGTCACCTGATTGC
ACCGGCAGAGGACTGGCATG

Downstream 100 bases:

>100_bases
CGGGATGCCGGCAATCTTACCCACAACTCCTCCCCCCAGCGAGGGAATCACCTTCAATTCCTCCCCCCAGCGGGGGGAGG
TTAGGAGGGGGGCGGAATTG

Product: cobalamin biosynthesis protein CobD

Products: ADP; phosphate; Adenosyl cobinamide phosphate; Adenosyl cobinamide

Alternate protein names: NA

Number of amino acids: Translated: 320; Mature: 320

Protein sequence:

>320_residues
MIGEELRRRCAIALLALVLDFALGDPPNQLHPVSLMGHWLKWGERLAPATPLARLAWGGGWLYGGWWLVGRVASRLPRHL
LVQGACVSLLLAYRGLDRAVAGVEQALRTGNLAEARHLLGWHLVSRPTADLSAAEVAGAAIESLAENLSDSIIAPLLAFL
VGGLPALAIYRLTNTADAMWGYRTERYEYLGKIAARCDDAFNLVPARLTALLLALATHLINGRGWHAWQTALRDARHTAS
PNAGWPMAAMAGALDTVLTKRDHYALGDGSCIPDAAMIGQARRIVQVAMAIVVSGLWIGILTGAGIRRAAPLHPDATLRG

Sequences:

>Translated_320_residues
MIGEELRRRCAIALLALVLDFALGDPPNQLHPVSLMGHWLKWGERLAPATPLARLAWGGGWLYGGWWLVGRVASRLPRHL
LVQGACVSLLLAYRGLDRAVAGVEQALRTGNLAEARHLLGWHLVSRPTADLSAAEVAGAAIESLAENLSDSIIAPLLAFL
VGGLPALAIYRLTNTADAMWGYRTERYEYLGKIAARCDDAFNLVPARLTALLLALATHLINGRGWHAWQTALRDARHTAS
PNAGWPMAAMAGALDTVLTKRDHYALGDGSCIPDAAMIGQARRIVQVAMAIVVSGLWIGILTGAGIRRAAPLHPDATLRG
>Mature_320_residues
MIGEELRRRCAIALLALVLDFALGDPPNQLHPVSLMGHWLKWGERLAPATPLARLAWGGGWLYGGWWLVGRVASRLPRHL
LVQGACVSLLLAYRGLDRAVAGVEQALRTGNLAEARHLLGWHLVSRPTADLSAAEVAGAAIESLAENLSDSIIAPLLAFL
VGGLPALAIYRLTNTADAMWGYRTERYEYLGKIAARCDDAFNLVPARLTALLLALATHLINGRGWHAWQTALRDARHTAS
PNAGWPMAAMAGALDTVLTKRDHYALGDGSCIPDAAMIGQARRIVQVAMAIVVSGLWIGILTGAGIRRAAPLHPDATLRG

Specific function: Converts cobyric acid to cobinamide by the addition of aminopropanol on the F carboxylic group [H]

COG id: COG1270

COG function: function code H; Cobalamin biosynthesis protein CobD/CbiB

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobD/CbiB family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004485 [H]

Pfam domain/function: PF03186 CobD_Cbib [H]

EC number: 6.3.1.10

Molecular weight: Translated: 34329; Mature: 34329

Theoretical pI: Translated: 9.48; Mature: 9.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIGEELRRRCAIALLALVLDFALGDPPNQLHPVSLMGHWLKWGERLAPATPLARLAWGGG
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHCCCC
WLYGGWWLVGRVASRLPRHLLVQGACVSLLLAYRGLDRAVAGVEQALRTGNLAEARHLLG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
WHLVSRPTADLSAAEVAGAAIESLAENLSDSIIAPLLAFLVGGLPALAIYRLTNTADAMW
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
GYRTERYEYLGKIAARCDDAFNLVPARLTALLLALATHLINGRGWHAWQTALRDARHTAS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC
PNAGWPMAAMAGALDTVLTKRDHYALGDGSCIPDAAMIGQARRIVQVAMAIVVSGLWIGI
CCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTGAGIRRAAPLHPDATLRG
HHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MIGEELRRRCAIALLALVLDFALGDPPNQLHPVSLMGHWLKWGERLAPATPLARLAWGGG
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHCCCC
WLYGGWWLVGRVASRLPRHLLVQGACVSLLLAYRGLDRAVAGVEQALRTGNLAEARHLLG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
WHLVSRPTADLSAAEVAGAAIESLAENLSDSIIAPLLAFLVGGLPALAIYRLTNTADAMW
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
GYRTERYEYLGKIAARCDDAFNLVPARLTALLLALATHLINGRGWHAWQTALRDARHTAS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC
PNAGWPMAAMAGALDTVLTKRDHYALGDGSCIPDAAMIGQARRIVQVAMAIVVSGLWIGI
CCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTGAGIRRAAPLHPDATLRG
HHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; adenosylcobyric acid; (R)-1-aminopropan-2-yl phosphate; (R)-1-aminopropan-2-ol

Specific reaction: (1) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-yl phosphate = ADP + phosphate + adenosylcobinamide phosphate; (2) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-ol = ADP + phosphate + adenosylcobinamide

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA