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The map label for this gene is cbiX [H]

Identifier: 222526023

GI number: 222526023

Start: 3423713

End: 3425107

Strand: Reverse

Name: cbiX [H]

Synonym: Chy400_2779

Alternate gene names: 222526023

Gene position: 3425107-3423713 (Counterclockwise)

Preceding gene: 222526024

Following gene: 222526022

Centisome position: 65.01

GC content: 59.93

Gene sequence:

>1395_bases
ATGACCAGAACAGCAATCTTACTGATCGGGCACGGCACTGATGACAGTGATGGTCTGGCCGAGTATCGCCAACTGGCCGG
CATGCTGCAAGAGCGGCTCGATCTCCCAGTGCAGCCATGCTTTCTCGAACTGGCCGATCCTCCGATTGGGCAGGCGATTG
ACGAGTGCGCGCGTGCCGGGTATCAACGTTTGATCGCGATACCGCTGCTCCTAGGCGCAGCCGGCCATCAGAAAAATGAT
ATTCCTGTTGCACTTCAGCAGGCGCGCCTGCGCTGGCCGGAACTTACCATTCAATACGGTACGCCACTCGGTGTGCAGTA
TTCACTGCTCAAAGCACTGGCCGACCGTCTGGCAACGGCAGAAGCAGCCGCCCCACCTGTACCACGTGCCGAAACCGCCC
TGGCCCTGATCGGGCGCGGCAGCAGTGACCCCGACAGTAACGCTGATGTTGCCCGCATGGCACGTTTGCTGTGGGAAGGT
CGTGGCTATGCTCACGTCACTTATGGCTTTTACAGCATTACTACACCGCGAGTACCGCAAACGCTCGATGCCTGCATCGC
GCTAGGTGCGCGTCGCATCATCGTGATCCCGTATCTGCTCTTCACCGGACGCATTCTGCGACGGATCATTGCTCAGGTCG
CTGCGGCCCGCCAAACATATCCCGATCACGACTTCGTTGTGACCGAACACCTTGGTCTGCACGAAGGTGTCCTTGCCGCC
ATCATGCAACGGGTACAGGAAGCCATGAATGGTCAGTCGGCGGCCAACTGTGACGTTTGTAAATATCGACGGCTCTTTCC
AGGGTTTACCGCCGACTTCGGGCGCCCCCAGACCAGCGATCACGAACACGGCTTACGCGGCGAAGCATCTGGCAGTATGA
CGAACATCCTCCCGCCTCGCTACCGTGGTGGCCGGGCAGTGAGCGCAGCGCCGATGACTGCCGCCCCGCTTGTCTTTGAC
GAACATGGGCAGGTCGCGTGGGATCGTATCTGGGGCGGTGATGATCCTGACAATCCCTTCTGCGAGCTGGCACTGGCCGG
TGGCCCGCCCCACCGGGGAACGCTGCTTGAGCCGGTCGATCCGGCAACGATCCAGGCCGACCCGGCAGGCTACGCCCGTG
TCATTGCCGAACTCCGCCGGGCATTGACGATGGTCACCGATCTGCCGGTAACGACCGACACCGCCCCTGGCTGGATCGGT
CTGGTCTGTGAAAGTGAAGAGATGGCAATCTGGCTCTTGCGGGCAATTATCGTGGAAAATGTCAGTGTCCGTCGCGAAGA
CCGCACGCTACTCTTACCGGCGGGGCCGCATTTTCGGCTGGAGGGAGAGATCAAAAATGTGGTCACGGCAGTTGCCAAGA
CCTATCACTACTGGAAGGAGCATATACAGCGATGA

Upstream 100 bases:

>100_bases
TCCCCCGACAGAAGCGGAGCAGGGGCTGGGGGGTAAAGTGAAGGGAGGAAGCCAGCCGACGTGTACACCATCTACAGCGT
AATGAACGGAGACCACGACG

Downstream 100 bases:

>100_bases
CAACGCCAACCTGCCGCATCTTCATCCTGCACGATCACGGTGAGCACGTGACCCAATTGGCGCAGGCGATTGGAGAGGGA
GCCGCCGGCGTTACCGGTGT

Product: cobalamin (vitamin B12) biosynthesis CbiX protein

Products: NA

Alternate protein names: CbiXL [H]

Number of amino acids: Translated: 464; Mature: 463

Protein sequence:

>464_residues
MTRTAILLIGHGTDDSDGLAEYRQLAGMLQERLDLPVQPCFLELADPPIGQAIDECARAGYQRLIAIPLLLGAAGHQKND
IPVALQQARLRWPELTIQYGTPLGVQYSLLKALADRLATAEAAAPPVPRAETALALIGRGSSDPDSNADVARMARLLWEG
RGYAHVTYGFYSITTPRVPQTLDACIALGARRIIVIPYLLFTGRILRRIIAQVAAARQTYPDHDFVVTEHLGLHEGVLAA
IMQRVQEAMNGQSAANCDVCKYRRLFPGFTADFGRPQTSDHEHGLRGEASGSMTNILPPRYRGGRAVSAAPMTAAPLVFD
EHGQVAWDRIWGGDDPDNPFCELALAGGPPHRGTLLEPVDPATIQADPAGYARVIAELRRALTMVTDLPVTTDTAPGWIG
LVCESEEMAIWLLRAIIVENVSVRREDRTLLLPAGPHFRLEGEIKNVVTAVAKTYHYWKEHIQR

Sequences:

>Translated_464_residues
MTRTAILLIGHGTDDSDGLAEYRQLAGMLQERLDLPVQPCFLELADPPIGQAIDECARAGYQRLIAIPLLLGAAGHQKND
IPVALQQARLRWPELTIQYGTPLGVQYSLLKALADRLATAEAAAPPVPRAETALALIGRGSSDPDSNADVARMARLLWEG
RGYAHVTYGFYSITTPRVPQTLDACIALGARRIIVIPYLLFTGRILRRIIAQVAAARQTYPDHDFVVTEHLGLHEGVLAA
IMQRVQEAMNGQSAANCDVCKYRRLFPGFTADFGRPQTSDHEHGLRGEASGSMTNILPPRYRGGRAVSAAPMTAAPLVFD
EHGQVAWDRIWGGDDPDNPFCELALAGGPPHRGTLLEPVDPATIQADPAGYARVIAELRRALTMVTDLPVTTDTAPGWIG
LVCESEEMAIWLLRAIIVENVSVRREDRTLLLPAGPHFRLEGEIKNVVTAVAKTYHYWKEHIQR
>Mature_463_residues
TRTAILLIGHGTDDSDGLAEYRQLAGMLQERLDLPVQPCFLELADPPIGQAIDECARAGYQRLIAIPLLLGAAGHQKNDI
PVALQQARLRWPELTIQYGTPLGVQYSLLKALADRLATAEAAAPPVPRAETALALIGRGSSDPDSNADVARMARLLWEGR
GYAHVTYGFYSITTPRVPQTLDACIALGARRIIVIPYLLFTGRILRRIIAQVAAARQTYPDHDFVVTEHLGLHEGVLAAI
MQRVQEAMNGQSAANCDVCKYRRLFPGFTADFGRPQTSDHEHGLRGEASGSMTNILPPRYRGGRAVSAAPMTAAPLVFDE
HGQVAWDRIWGGDDPDNPFCELALAGGPPHRGTLLEPVDPATIQADPAGYARVIAELRRALTMVTDLPVTTDTAPGWIGL
VCESEEMAIWLLRAIIVENVSVRREDRTLLLPAGPHFRLEGEIKNVVTAVAKTYHYWKEHIQR

Specific function: Chelates cobalt to the cobalamin precursor as part of the anaerobic pathway to cobalamin biosynthesis [H]

COG id: COG2138

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CbiX family. CbiXL subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002762 [H]

Pfam domain/function: PF01903 CbiX [H]

EC number: =4.99.1.3 [H]

Molecular weight: Translated: 50751; Mature: 50620

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRTAILLIGHGTDDSDGLAEYRQLAGMLQERLDLPVQPCFLELADPPIGQAIDECARAG
CCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCHHHHHHHHHHHH
YQRLIAIPLLLGAAGHQKNDIPVALQQARLRWPELTIQYGTPLGVQYSLLKALADRLATA
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHH
EAAAPPVPRAETALALIGRGSSDPDSNADVARMARLLWEGRGYAHVTYGFYSITTPRVPQ
HHCCCCCCCHHHHHHEEECCCCCCCCCHHHHHHHHHHHCCCCEEEEEEEEEEECCCCCHH
TLDACIALGARRIIVIPYLLFTGRILRRIIAQVAAARQTYPDHDFVVTEHLGLHEGVLAA
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHH
IMQRVQEAMNGQSAANCDVCKYRRLFPGFTADFGRPQTSDHEHGLRGEASGSMTNILPPR
HHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
YRGGRAVSAAPMTAAPLVFDEHGQVAWDRIWGGDDPDNPFCELALAGGPPHRGTLLEPVD
CCCCEEECCCCCCCCCEEECCCCCEEEHHCCCCCCCCCCCEEEEECCCCCCCCCCCCCCC
PATIQADPAGYARVIAELRRALTMVTDLPVTTDTAPGWIGLVCESEEMAIWLLRAIIVEN
CCEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHC
VSVRREDRTLLLPAGPHFRLEGEIKNVVTAVAKTYHYWKEHIQR
CCEECCCCEEEEECCCCEEECHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TRTAILLIGHGTDDSDGLAEYRQLAGMLQERLDLPVQPCFLELADPPIGQAIDECARAG
CCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCHHHHHHHHHHHH
YQRLIAIPLLLGAAGHQKNDIPVALQQARLRWPELTIQYGTPLGVQYSLLKALADRLATA
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHH
EAAAPPVPRAETALALIGRGSSDPDSNADVARMARLLWEGRGYAHVTYGFYSITTPRVPQ
HHCCCCCCCHHHHHHEEECCCCCCCCCHHHHHHHHHHHCCCCEEEEEEEEEEECCCCCHH
TLDACIALGARRIIVIPYLLFTGRILRRIIAQVAAARQTYPDHDFVVTEHLGLHEGVLAA
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHH
IMQRVQEAMNGQSAANCDVCKYRRLFPGFTADFGRPQTSDHEHGLRGEASGSMTNILPPR
HHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
YRGGRAVSAAPMTAAPLVFDEHGQVAWDRIWGGDDPDNPFCELALAGGPPHRGTLLEPVD
CCCCEEECCCCCCCCCEEECCCCCEEEHHCCCCCCCCCCCEEEEECCCCCCCCCCCCCCC
PATIQADPAGYARVIAELRRALTMVTDLPVTTDTAPGWIGLVCESEEMAIWLLRAIIVEN
CCEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHC
VSVRREDRTLLLPAGPHFRLEGEIKNVVTAVAKTYHYWKEHIQR
CCEECCCCEEEEECCCCEEECHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9742225; 12917443 [H]