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The map label for this gene is mutB [H]

Identifier: 222525949

GI number: 222525949

Start: 3336976

End: 3339177

Strand: Reverse

Name: mutB [H]

Synonym: Chy400_2705

Alternate gene names: 222525949

Gene position: 3339177-3336976 (Counterclockwise)

Preceding gene: 222525950

Following gene: 222525948

Centisome position: 63.37

GC content: 60.04

Gene sequence:

>2202_bases
GTGAAGAACCCCGACTTTACTACCCTATCGTACCGTGATCCATGGCCTACATCCGATCTTGGCCAGTGGCAGGAACGCGC
TGAGCAGGAGGCCGGCCAACCTCTGGCCGATCTGGTCTGGCGGACGATGGAACAGATCGAGGTGAGACCGCTCTACACCG
CAGCCGATCTGGCCGGCCTGGAGCACCTTGGCTTTACCGCCGGTATCCCCCCGTACCTGCGCGGGCCGTATCCTACCATG
TACGTCACCCAACCCTGGACAATCCGCCAGTACGCCGGTTTCTCGACCGCCGAAGCCAGCAATGCCTTCTACCGGCGAAA
TCTGGCTGCCGGTCAAAAGGGCTTATCAGTCGCCTTCGACCTGGCGACCCACCGCGGCTATGACTCCGACCATCCGCGGG
TGGTGGGTGACGTTGGTAAAGCGGGTGTCGCCATCGACTCCGTGCTCGACATGAAAATACTCTTCGACGGCATTCCGCTC
GATCAGATGTCGGTTTCGATGACGATGAACGGTGCTGTGATCCCGATCATGGCCTTCTTCATCGTCGCCGCTGAAGAGCA
AGGGGTGCGCCCGGAACAACTCACCGGCACAATCCAAAACGACATTCTGAAAGAGTACATGGTGCGCAACACCTACATCT
ACCCACCCGAACCGTCAATGCGCATCATTGCCGACATCTTCGCCTACACGGCGCAGCACATGCCCAAATTCAACAGCATC
AGCATCTCCGGCTACCATATGCAAGAGGCTGGTGCCAGCGCCGACCTTGAACTGGCCTACACGCTGGCCGACGGGCTGGA
ATACGTGCGCGCCGGCCTGAAAGCCGGCCTGGCAATCGACAGCTTTGCGCCGCGCCTCTCCTTCTTCTGGGCCATCGGCA
TGAACTACTTCATGGAGATTGCCAAGATGCGGGCAGCACGTCTGCTGTGGGCAAAAATCATCAAACAGTTCAACCCGCAA
GACCCGCGCTCGCTGGCGCTCCGCACCCATTGTCAGACCTCCGGCTGGAGCCTGACCGAGCAAGACCCCTTCAACAATGT
GGCACGCACCTGCATTGAGGCGATGGCGGCGGCGCTCGGTCACACCCAATCGCTGCACACCAATTCACTCGACGAAGCGA
TTGCCTTGCCGACCGATTTTTCGGCCCGCATCGCCCGCAACACCCAGTTGTACCTGCAAGAGGAGACCGGCATCTGCAAG
ATCGTCGATCCGTGGGGCGGTTCGTACTACCTCGAATGGCTCACCGACGCATTGGCCCGCCGGGCCTGGACGCATATCCA
GGAAGTCGAGGCCCTCGGTGGGATGGCCAAAGCGATAGAGGCCGGCTTGCCCAAATTACGGATCGAAGAAGCAGCGGCCC
GTCGGCAAGCCCACATCGACTCTGGGCGAGAGACGATTGTCGGGGTGAACAAATATCGTCTCGATTACGAAGAACCCATC
GAGATTCTGGAAGTCGATAACACCGCTGTGCGCCAGAAGCAGATCGAACGCCTGCAACGCCTGCGTACCGAACGTGACGA
AGTCCGTGTTCAAGCCACGCTCAACGAATTGACCCGCGTGGCGGCCTCTGGTGAAGGCAACCTGCTCGCTGCGGCCATCG
AAGCGGCCCGCGCTCGCGCCACACTTGGCGAGATTTCGCTGGCAATGGAAAAGGTGTTTGGGCGGTTCAAGGCTGAAATT
CGCTCCATTTCTGGTGTCTACAGCAGCGAATACAGCGATGTAGAACAGATTCACCACGTGCGGGCAATGGCCGACGCCTT
CGCCGCTCTGGAAGGGCGCCGACCGCGCATACTGGTCGCCAAAATCGGCCAGGACGGTCACGACCGGGGGGCCAAGGTGG
TCGCTACCGCATTTGCCGATCTCGGCTTCGATGTGGATATTGGTTCACTCTTCCAGACACCGGAAGAAGTGGCGCGTCAG
GCGGTAGAAAATGACGTACACGTGGTGGGGATCAGCTCACTGGCCGGCGGTCACAAGACTCTCTTGCCCCAACTGGTCGA
GGAGTTGCGCAAACTGGGCCGCGAAGACATCATGGTCGTGATCGGTGGTATCATCCCACCACAAGACTACGACTTTCTCC
GCGCCCACGGTGCAGCCCTGATCTTCGGCCCCGGAACCATCATTCCGGTCGCTGCCGAGAAGCTGTTGCGCGAGTTGCAA
CGGCGTCTCCACGGTGATAGCGTCGCTGCACCGGCATCGTGA

Upstream 100 bases:

>100_bases
TTGACACGTACCGCGCTGCCGGGATCGATGAATTCATCCATCTACGCGCCAATTGCTACGACATCCTGGCCCGGCTGCAA
CGCTTGAAAGGAGTGGCCGC

Downstream 100 bases:

>100_bases
AACGAGGGTGAGAGGGATCATGACCGATAAGCAGCAGCCTTCATCTTCTGGCGAACTACCATTTGCGCTATCGGTACTGG
CCGGGATTGCCAGCCGTCAC

Product: methylmalonyl-CoA mutase

Products: NA

Alternate protein names: MCM-alpha [H]

Number of amino acids: Translated: 733; Mature: 733

Protein sequence:

>733_residues
MKNPDFTTLSYRDPWPTSDLGQWQERAEQEAGQPLADLVWRTMEQIEVRPLYTAADLAGLEHLGFTAGIPPYLRGPYPTM
YVTQPWTIRQYAGFSTAEASNAFYRRNLAAGQKGLSVAFDLATHRGYDSDHPRVVGDVGKAGVAIDSVLDMKILFDGIPL
DQMSVSMTMNGAVIPIMAFFIVAAEEQGVRPEQLTGTIQNDILKEYMVRNTYIYPPEPSMRIIADIFAYTAQHMPKFNSI
SISGYHMQEAGASADLELAYTLADGLEYVRAGLKAGLAIDSFAPRLSFFWAIGMNYFMEIAKMRAARLLWAKIIKQFNPQ
DPRSLALRTHCQTSGWSLTEQDPFNNVARTCIEAMAAALGHTQSLHTNSLDEAIALPTDFSARIARNTQLYLQEETGICK
IVDPWGGSYYLEWLTDALARRAWTHIQEVEALGGMAKAIEAGLPKLRIEEAAARRQAHIDSGRETIVGVNKYRLDYEEPI
EILEVDNTAVRQKQIERLQRLRTERDEVRVQATLNELTRVAASGEGNLLAAAIEAARARATLGEISLAMEKVFGRFKAEI
RSISGVYSSEYSDVEQIHHVRAMADAFAALEGRRPRILVAKIGQDGHDRGAKVVATAFADLGFDVDIGSLFQTPEEVARQ
AVENDVHVVGISSLAGGHKTLLPQLVEELRKLGREDIMVVIGGIIPPQDYDFLRAHGAALIFGPGTIIPVAAEKLLRELQ
RRLHGDSVAAPAS

Sequences:

>Translated_733_residues
MKNPDFTTLSYRDPWPTSDLGQWQERAEQEAGQPLADLVWRTMEQIEVRPLYTAADLAGLEHLGFTAGIPPYLRGPYPTM
YVTQPWTIRQYAGFSTAEASNAFYRRNLAAGQKGLSVAFDLATHRGYDSDHPRVVGDVGKAGVAIDSVLDMKILFDGIPL
DQMSVSMTMNGAVIPIMAFFIVAAEEQGVRPEQLTGTIQNDILKEYMVRNTYIYPPEPSMRIIADIFAYTAQHMPKFNSI
SISGYHMQEAGASADLELAYTLADGLEYVRAGLKAGLAIDSFAPRLSFFWAIGMNYFMEIAKMRAARLLWAKIIKQFNPQ
DPRSLALRTHCQTSGWSLTEQDPFNNVARTCIEAMAAALGHTQSLHTNSLDEAIALPTDFSARIARNTQLYLQEETGICK
IVDPWGGSYYLEWLTDALARRAWTHIQEVEALGGMAKAIEAGLPKLRIEEAAARRQAHIDSGRETIVGVNKYRLDYEEPI
EILEVDNTAVRQKQIERLQRLRTERDEVRVQATLNELTRVAASGEGNLLAAAIEAARARATLGEISLAMEKVFGRFKAEI
RSISGVYSSEYSDVEQIHHVRAMADAFAALEGRRPRILVAKIGQDGHDRGAKVVATAFADLGFDVDIGSLFQTPEEVARQ
AVENDVHVVGISSLAGGHKTLLPQLVEELRKLGREDIMVVIGGIIPPQDYDFLRAHGAALIFGPGTIIPVAAEKLLRELQ
RRLHGDSVAAPAS
>Mature_733_residues
MKNPDFTTLSYRDPWPTSDLGQWQERAEQEAGQPLADLVWRTMEQIEVRPLYTAADLAGLEHLGFTAGIPPYLRGPYPTM
YVTQPWTIRQYAGFSTAEASNAFYRRNLAAGQKGLSVAFDLATHRGYDSDHPRVVGDVGKAGVAIDSVLDMKILFDGIPL
DQMSVSMTMNGAVIPIMAFFIVAAEEQGVRPEQLTGTIQNDILKEYMVRNTYIYPPEPSMRIIADIFAYTAQHMPKFNSI
SISGYHMQEAGASADLELAYTLADGLEYVRAGLKAGLAIDSFAPRLSFFWAIGMNYFMEIAKMRAARLLWAKIIKQFNPQ
DPRSLALRTHCQTSGWSLTEQDPFNNVARTCIEAMAAALGHTQSLHTNSLDEAIALPTDFSARIARNTQLYLQEETGICK
IVDPWGGSYYLEWLTDALARRAWTHIQEVEALGGMAKAIEAGLPKLRIEEAAARRQAHIDSGRETIVGVNKYRLDYEEPI
EILEVDNTAVRQKQIERLQRLRTERDEVRVQATLNELTRVAASGEGNLLAAAIEAARARATLGEISLAMEKVFGRFKAEI
RSISGVYSSEYSDVEQIHHVRAMADAFAALEGRRPRILVAKIGQDGHDRGAKVVATAFADLGFDVDIGSLFQTPEEVARQ
AVENDVHVVGISSLAGGHKTLLPQLVEELRKLGREDIMVVIGGIIPPQDYDFLRAHGAALIFGPGTIIPVAAEKLLRELQ
RRLHGDSVAAPAS

Specific function: Catalyzes the isomerization of succinyl-CoA to methylmalonyl-CoA during synthesis of propionate from tricarboxylic acid-cycle intermediates [H]

COG id: COG1884

COG function: function code I; Methylmalonyl-CoA mutase, N-terminal domain/subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 B12-binding domain [H]

Homologues:

Organism=Homo sapiens, GI156105689, Length=705, Percent_Identity=65.2482269503546, Blast_Score=956, Evalue=0.0,
Organism=Escherichia coli, GI1789284, Length=696, Percent_Identity=64.6551724137931, Blast_Score=914, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25144500, Length=705, Percent_Identity=62.6950354609929, Blast_Score=924, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006159
- InterPro:   IPR016176
- InterPro:   IPR014348
- InterPro:   IPR006158
- InterPro:   IPR006099
- InterPro:   IPR006098 [H]

Pfam domain/function: PF02310 B12-binding; PF01642 MM_CoA_mutase [H]

EC number: =5.4.99.2 [H]

Molecular weight: Translated: 80996; Mature: 80996

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: PS00544 METMALONYL_COA_MUTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNPDFTTLSYRDPWPTSDLGQWQERAEQEAGQPLADLVWRTMEQIEVRPLYTAADLAGL
CCCCCCCEEECCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHH
EHLGFTAGIPPYLRGPYPTMYVTQPWTIRQYAGFSTAEASNAFYRRNLAAGQKGLSVAFD
HHCCCCCCCCCCCCCCCCEEEEECCEEEHHHCCCCCHHHHHHHHHHHHHCCCCCCEEEEE
LATHRGYDSDHPRVVGDVGKAGVAIDSVLDMKILFDGIPLDQMSVSMTMNGAVIPIMAFF
HHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEECCCHHHHHHHH
IVAAEEQGVRPEQLTGTIQNDILKEYMVRNTYIYPPEPSMRIIADIFAYTAQHMPKFNSI
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEECCCCCHHHHHHHHHHHHHHCCCCCCE
SISGYHMQEAGASADLELAYTLADGLEYVRAGLKAGLAIDSFAPRLSFFWAIGMNYFMEI
EEECEEHHHCCCCCCCEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
AKMRAARLLWAKIIKQFNPQDPRSLALRTHCQTSGWSLTEQDPFNNVARTCIEAMAAALG
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHC
HTQSLHTNSLDEAIALPTDFSARIARNTQLYLQEETGICKIVDPWGGSYYLEWLTDALAR
CCCCCCCCCCHHHHCCCCCCHHHHHCCCEEEEEECCCEEEEECCCCCHHHHHHHHHHHHH
RAWTHIQEVEALGGMAKAIEAGLPKLRIEEAAARRQAHIDSGRETIVGVNKYRLDYEEPI
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCEEEECHHEECCCCCCC
EILEVDNTAVRQKQIERLQRLRTERDEVRVQATLNELTRVAASGEGNLLAAAIEAARARA
EEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
TLGEISLAMEKVFGRFKAEIRSISGVYSSEYSDVEQIHHVRAMADAFAALEGRRPRILVA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
KIGQDGHDRGAKVVATAFADLGFDVDIGSLFQTPEEVARQAVENDVHVVGISSLAGGHKT
ECCCCCCCCCHHHHHHHHHHCCCCCCHHHHHCCHHHHHHHHHHCCEEEEEEHHHCCCHHH
LLPQLVEELRKLGREDIMVVIGGIIPPQDYDFLRAHGAALIFGPGTIIPVAAEKLLRELQ
HHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHCCCEEEECCCCCHHHHHHHHHHHHH
RRLHGDSVAAPAS
HHHCCCCCCCCCC
>Mature Secondary Structure
MKNPDFTTLSYRDPWPTSDLGQWQERAEQEAGQPLADLVWRTMEQIEVRPLYTAADLAGL
CCCCCCCEEECCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHH
EHLGFTAGIPPYLRGPYPTMYVTQPWTIRQYAGFSTAEASNAFYRRNLAAGQKGLSVAFD
HHCCCCCCCCCCCCCCCCEEEEECCEEEHHHCCCCCHHHHHHHHHHHHHCCCCCCEEEEE
LATHRGYDSDHPRVVGDVGKAGVAIDSVLDMKILFDGIPLDQMSVSMTMNGAVIPIMAFF
HHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEECCCHHHHHHHH
IVAAEEQGVRPEQLTGTIQNDILKEYMVRNTYIYPPEPSMRIIADIFAYTAQHMPKFNSI
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEECCCCCHHHHHHHHHHHHHHCCCCCCE
SISGYHMQEAGASADLELAYTLADGLEYVRAGLKAGLAIDSFAPRLSFFWAIGMNYFMEI
EEECEEHHHCCCCCCCEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
AKMRAARLLWAKIIKQFNPQDPRSLALRTHCQTSGWSLTEQDPFNNVARTCIEAMAAALG
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHC
HTQSLHTNSLDEAIALPTDFSARIARNTQLYLQEETGICKIVDPWGGSYYLEWLTDALAR
CCCCCCCCCCHHHHCCCCCCHHHHHCCCEEEEEECCCEEEEECCCCCHHHHHHHHHHHHH
RAWTHIQEVEALGGMAKAIEAGLPKLRIEEAAARRQAHIDSGRETIVGVNKYRLDYEEPI
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCEEEECHHEECCCCCCC
EILEVDNTAVRQKQIERLQRLRTERDEVRVQATLNELTRVAASGEGNLLAAAIEAARARA
EEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
TLGEISLAMEKVFGRFKAEIRSISGVYSSEYSDVEQIHHVRAMADAFAALEGRRPRILVA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
KIGQDGHDRGAKVVATAFADLGFDVDIGSLFQTPEEVARQAVENDVHVVGISSLAGGHKT
ECCCCCCCCCHHHHHHHHHHCCCCCCHHHHHCCHHHHHHHHHHCCEEEEEEHHHCCCHHH
LLPQLVEELRKLGREDIMVVIGGIIPPQDYDFLRAHGAALIFGPGTIIPVAAEKLLRELQ
HHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHCCCEEEECCCCCHHHHHHHHHHHHH
RRLHGDSVAAPAS
HHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8566763; 12949112 [H]