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The map label for this gene is hofB [C]

Identifier: 222525845

GI number: 222525845

Start: 3233183

End: 3234646

Strand: Reverse

Name: hofB [C]

Synonym: Chy400_2598

Alternate gene names: 222525845

Gene position: 3234646-3233183 (Counterclockwise)

Preceding gene: 222525846

Following gene: 222525844

Centisome position: 61.39

GC content: 55.74

Gene sequence:

>1464_bases
ATGTCGCTGCTCAAACGTCTCGGTAATCAACCACAGCCAGAGCCTGCTCCGGCTGTTGCTACACCCCCGCCTGTAGCGGC
AACCGTGGTGCCGGCTCCAGCGCCGCCTCCACCGGTGACGCCCCCTGCTGCCCCAGCACCAGCTCGCCCAACGGTTCAGG
ACGCAACTCCGGCCAATGATCAGAAACGGATGCTGGAACTGTCGCTCTGGATTGTTGACCGCATTCAGGCTTCGTTGGGG
AATCAAACGGAGCTGAAGCGATCTCCTGAGACGGAGCGACTACTCCAAGAGCGCTTCGCTCGTATTTATCAACAGGCGAA
CGTGCAACTTTCCGACCAGGATGTCAAAAAGCTCTTCGCAATGGTCTGCGATGAGCTGTTCGGCTTCGGCCCACTTCAGC
AGTTGCTCGACGATGATAGCATTTCCGAGGTGATGGTCAATGGACCATACAAGGTCTACATTGAACAAAAGGGCAAGCTC
AAGCTCTCTGATGTACGTTTTGCATCTGATGAGCATGTGATGAAGATTATCGACCGGATCATCCGGCCGCTCGGTCGCCG
CATCGACCGCAAGTGGCCGATGGTCGATGCTCGTTTGCCCGATGGCTCGCGTGTTAATGCGATCATCCCGCCCTGCGCTA
TCGATGGCTCAACGATTACCATTCGTAAGTTTTCCAAGAACAAGCTCAAGGTCGATGACCTGATTCGCTTCGGTTCGATG
ACGGCTGAAATGGCAGAATTTCTCCGTGCCTGTGTCGTCTCGCGCTTGAACATTGTGGTTGCTGGTGGTACCGGCTCCGG
TAAGACGACATTGCTGAACGTGCTGTCGAACTTTATTCCCGAAGACGAACGTATCGTAACGATTGAAGACAGCGCCGAGC
TTCAACTGGCCCAGGATCACGTGGTTCGCCTCGAAGCCAAACCACCGGAGATCGACGGTACCGGGCGGGTGACGATCCGC
GACCTGGTGATCAACTCACTGCGTATGCGACCGGAGCGGATTGTGATTGGCGAATGCCGTGGTGGTGAGACGCTTGACAT
GTTGCAGGCAATGAATACCGGCCACGATGGCTCGTTGACTACCCTGCACGCAAATACGCCACGCGACGCTATCGCTCGTA
TGGAAACAATGGCGCTGATGGCGGGTATGGAAATGCCGCTAAAGGTTATTCGTGAACAGATCGCCTCGGCAATTGATCTG
ATTGTCCAGCAAACCCGCCTCGAAGATGGTTCACGCAAGGTGGCCTATATCACCGAAGTGCAGGGCATGGAAGGTGATAC
CGTCGTTTTGCAAGACATCTTTAAGCTCGAGATTCTGGGCAAGACGGGTGAAGGTAAGATACTGGCCGAATTACGCCCAA
CCGGTGTCCGGCCCAAGTTTACACCTCGTCTGGAGGCTCATGGCTTCAAACTGCCGCCCAGCATCTTCGGCGCTCAGATA
CCCGGTCAGCGCGGTCGCTTCTAG

Upstream 100 bases:

>100_bases
CTTGGTTAAACATCCATCCAGATAAGGTGCTTCGTGTACGCGCCAGTGCTACTGACGTTCGGCGAGAGTTCGGATGGACA
ACTAAGGAGGTTTTATCGCT

Downstream 100 bases:

>100_bases
TTAACCGGTACACCGGGTTTGTGTTAGAGATTGACAGTCTCAGGGTAGCCATTTGGCTGCCCTTGTCTTTTGGTTGCCTC
TGTGATGGTACGCAAAGCCC

Product: type II secretion system protein E

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 487; Mature: 486

Protein sequence:

>487_residues
MSLLKRLGNQPQPEPAPAVATPPPVAATVVPAPAPPPPVTPPAAPAPARPTVQDATPANDQKRMLELSLWIVDRIQASLG
NQTELKRSPETERLLQERFARIYQQANVQLSDQDVKKLFAMVCDELFGFGPLQQLLDDDSISEVMVNGPYKVYIEQKGKL
KLSDVRFASDEHVMKIIDRIIRPLGRRIDRKWPMVDARLPDGSRVNAIIPPCAIDGSTITIRKFSKNKLKVDDLIRFGSM
TAEMAEFLRACVVSRLNIVVAGGTGSGKTTLLNVLSNFIPEDERIVTIEDSAELQLAQDHVVRLEAKPPEIDGTGRVTIR
DLVINSLRMRPERIVIGECRGGETLDMLQAMNTGHDGSLTTLHANTPRDAIARMETMALMAGMEMPLKVIREQIASAIDL
IVQQTRLEDGSRKVAYITEVQGMEGDTVVLQDIFKLEILGKTGEGKILAELRPTGVRPKFTPRLEAHGFKLPPSIFGAQI
PGQRGRF

Sequences:

>Translated_487_residues
MSLLKRLGNQPQPEPAPAVATPPPVAATVVPAPAPPPPVTPPAAPAPARPTVQDATPANDQKRMLELSLWIVDRIQASLG
NQTELKRSPETERLLQERFARIYQQANVQLSDQDVKKLFAMVCDELFGFGPLQQLLDDDSISEVMVNGPYKVYIEQKGKL
KLSDVRFASDEHVMKIIDRIIRPLGRRIDRKWPMVDARLPDGSRVNAIIPPCAIDGSTITIRKFSKNKLKVDDLIRFGSM
TAEMAEFLRACVVSRLNIVVAGGTGSGKTTLLNVLSNFIPEDERIVTIEDSAELQLAQDHVVRLEAKPPEIDGTGRVTIR
DLVINSLRMRPERIVIGECRGGETLDMLQAMNTGHDGSLTTLHANTPRDAIARMETMALMAGMEMPLKVIREQIASAIDL
IVQQTRLEDGSRKVAYITEVQGMEGDTVVLQDIFKLEILGKTGEGKILAELRPTGVRPKFTPRLEAHGFKLPPSIFGAQI
PGQRGRF
>Mature_486_residues
SLLKRLGNQPQPEPAPAVATPPPVAATVVPAPAPPPPVTPPAAPAPARPTVQDATPANDQKRMLELSLWIVDRIQASLGN
QTELKRSPETERLLQERFARIYQQANVQLSDQDVKKLFAMVCDELFGFGPLQQLLDDDSISEVMVNGPYKVYIEQKGKLK
LSDVRFASDEHVMKIIDRIIRPLGRRIDRKWPMVDARLPDGSRVNAIIPPCAIDGSTITIRKFSKNKLKVDDLIRFGSMT
AEMAEFLRACVVSRLNIVVAGGTGSGKTTLLNVLSNFIPEDERIVTIEDSAELQLAQDHVVRLEAKPPEIDGTGRVTIRD
LVINSLRMRPERIVIGECRGGETLDMLQAMNTGHDGSLTTLHANTPRDAIARMETMALMAGMEMPLKVIREQIASAIDLI
VQQTRLEDGSRKVAYITEVQGMEGDTVVLQDIFKLEILGKTGEGKILAELRPTGVRPKFTPRLEAHGFKLPPSIFGAQIP
GQRGRF

Specific function: Unknown

COG id: COG4962

COG function: function code U; Flp pilus assembly protein, ATPase CpaF

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1786296, Length=174, Percent_Identity=29.8850574712644, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022399
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 53679; Mature: 53548

Theoretical pI: Translated: 7.97; Mature: 7.97

Prosite motif: PS00227 TUBULIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLLKRLGNQPQPEPAPAVATPPPVAATVVPAPAPPPPVTPPAAPAPARPTVQDATPAND
CCHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCH
QKRMLELSLWIVDRIQASLGNQTELKRSPETERLLQERFARIYQQANVQLSDQDVKKLFA
HHHHHHHHHHHHHHHHHHCCCCHHHCCCCCHHHHHHHHHHHHHHHCCCEECHHHHHHHHH
MVCDELFGFGPLQQLLDDDSISEVMVNGPYKVYIEQKGKLKLSDVRFASDEHVMKIIDRI
HHHHHHHCCCHHHHHHCCCCHHHEEECCCEEEEEECCCCEEEECEEECCHHHHHHHHHHH
IRPLGRRIDRKWPMVDARLPDGSRVNAIIPPCAIDGSTITIRKFSKNKLKVDDLIRFGSM
HHHHHHHHCCCCCEEECCCCCCCCEEEECCCCCCCCCEEEEEECCCCCCCHHHHHHHCCH
TAEMAEFLRACVVSRLNIVVAGGTGSGKTTLLNVLSNFIPEDERIVTIEDSAELQLAQDH
HHHHHHHHHHHHHHHCEEEEECCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCEEEECCC
VVRLEAKPPEIDGTGRVTIRDLVINSLRMRPERIVIGECRGGETLDMLQAMNTGHDGSLT
EEEEECCCCCCCCCCCEEHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHCCCCCCCEE
TLHANTPRDAIARMETMALMAGMEMPLKVIREQIASAIDLIVQQTRLEDGSRKVAYITEV
EEECCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEE
QGMEGDTVVLQDIFKLEILGKTGEGKILAELRPTGVRPKFTPRLEAHGFKLPPSIFGAQI
CCCCCCCHHHHHHHEEEEECCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCHHHHCCCC
PGQRGRF
CCCCCCC
>Mature Secondary Structure 
SLLKRLGNQPQPEPAPAVATPPPVAATVVPAPAPPPPVTPPAAPAPARPTVQDATPAND
CHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCH
QKRMLELSLWIVDRIQASLGNQTELKRSPETERLLQERFARIYQQANVQLSDQDVKKLFA
HHHHHHHHHHHHHHHHHHCCCCHHHCCCCCHHHHHHHHHHHHHHHCCCEECHHHHHHHHH
MVCDELFGFGPLQQLLDDDSISEVMVNGPYKVYIEQKGKLKLSDVRFASDEHVMKIIDRI
HHHHHHHCCCHHHHHHCCCCHHHEEECCCEEEEEECCCCEEEECEEECCHHHHHHHHHHH
IRPLGRRIDRKWPMVDARLPDGSRVNAIIPPCAIDGSTITIRKFSKNKLKVDDLIRFGSM
HHHHHHHHCCCCCEEECCCCCCCCEEEECCCCCCCCCEEEEEECCCCCCCHHHHHHHCCH
TAEMAEFLRACVVSRLNIVVAGGTGSGKTTLLNVLSNFIPEDERIVTIEDSAELQLAQDH
HHHHHHHHHHHHHHHCEEEEECCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCEEEECCC
VVRLEAKPPEIDGTGRVTIRDLVINSLRMRPERIVIGECRGGETLDMLQAMNTGHDGSLT
EEEEECCCCCCCCCCCEEHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHCCCCCCCEE
TLHANTPRDAIARMETMALMAGMEMPLKVIREQIASAIDLIVQQTRLEDGSRKVAYITEV
EEECCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEE
QGMEGDTVVLQDIFKLEILGKTGEGKILAELRPTGVRPKFTPRLEAHGFKLPPSIFGAQI
CCCCCCCHHHHHHHEEEEECCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCHHHHCCCC
PGQRGRF
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12218036; 9634230 [H]