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The map label for this gene is truA

Identifier: 222525832

GI number: 222525832

Start: 3222792

End: 3223628

Strand: Direct

Name: truA

Synonym: Chy400_2585

Alternate gene names: 222525832

Gene position: 3222792-3223628 (Clockwise)

Preceding gene: 222525831

Following gene: 222525833

Centisome position: 61.17

GC content: 56.75

Gene sequence:

>837_bases
ATGCGCACGATTGCGCTGCTGTTAGCTTATGACGGCACCGATTTTGCCGGGTCGCAATGGCAAACTGACATCCGAACTGT
GCAGGGTGCCCTGGAGTCGGCATGGGAAGCGTTGACGCAAGAGCGGCGTCGGATTGTGCTGGCAGGCCGAACCGATGCCG
GAGTTCACGCGACTGGTCAGGTCGCTCACGTCCAGACCACAACGCGCCACAATTTACAAACAATCTGGCGCGGTTTGAAC
GCTCACCTGCCGGTTGACCTGGCCATTCAGAATGTCGGGGAAGCAGTACCAGATTTTCACGCTCGCTTTAGTGCGATCCA
GCGTGAGTATCGTTACCTGATAGACTGTGCAGCAGCGCCGTTGCCACAACTTCGCCATCAAGTCTTGCACTACGCAGGTA
CACTTGATGTGGCGGCAATGACGGCAGCGCTCAAATTGCTGGAGGGAACGCACGATTTTGCTGCATTTACAACAGCAACG
CCTGCCCAACGTTCTACCGTTCGCACCATGTATTGGACGCGGGTTGGGGATTATGAATGGTTTGAGCGGCGTTTACTGGC
TATCGAAGTAGCGGCAAATGCATTCCTCCAGCATATGGTGCGGATGATCGTCGGTACGCTCTTGCTGGTTGGGCGTGGGC
GGATGACCGTCGATCAGTTTGGAGAGGTGTTGGCGAGTCGGGATCGCCGCCTGGCTGGTCCAACGGCGCCGGCTCACGGC
TTAACATTAACGGCAGTACGCTATCCGCCTGGCCTGATCCGCTGGGTGGAACCTTCAAAACGGCAAAATGGCACGACGAA
GGTCGAACAGCCTTCGTCCTACGTACACGAGGAATAG

Upstream 100 bases:

>100_bases
CTACCACCTACGGTGGCCGGAATGGTGGGTACACCCGGATCACAAAACTGGGACCACGCCGGGGTGATGGAGCCGAGATG
GCATTGATTGAGTTGATCTG

Downstream 100 bases:

>100_bases
GTGCTGTGAAGACCTATCATCAAAAACCAACAGAAGTGCAGCGTGATTGGTATGTCATCGATGCGAGTGGTAAGGTGCTC
GGTCGGCTGGCAACACAGAT

Product: tRNA pseudouridine synthase ACD

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I

Number of amino acids: Translated: 278; Mature: 278

Protein sequence:

>278_residues
MRTIALLLAYDGTDFAGSQWQTDIRTVQGALESAWEALTQERRRIVLAGRTDAGVHATGQVAHVQTTTRHNLQTIWRGLN
AHLPVDLAIQNVGEAVPDFHARFSAIQREYRYLIDCAAAPLPQLRHQVLHYAGTLDVAAMTAALKLLEGTHDFAAFTTAT
PAQRSTVRTMYWTRVGDYEWFERRLLAIEVAANAFLQHMVRMIVGTLLLVGRGRMTVDQFGEVLASRDRRLAGPTAPAHG
LTLTAVRYPPGLIRWVEPSKRQNGTTKVEQPSSYVHEE

Sequences:

>Translated_278_residues
MRTIALLLAYDGTDFAGSQWQTDIRTVQGALESAWEALTQERRRIVLAGRTDAGVHATGQVAHVQTTTRHNLQTIWRGLN
AHLPVDLAIQNVGEAVPDFHARFSAIQREYRYLIDCAAAPLPQLRHQVLHYAGTLDVAAMTAALKLLEGTHDFAAFTTAT
PAQRSTVRTMYWTRVGDYEWFERRLLAIEVAANAFLQHMVRMIVGTLLLVGRGRMTVDQFGEVLASRDRRLAGPTAPAHG
LTLTAVRYPPGLIRWVEPSKRQNGTTKVEQPSSYVHEE
>Mature_278_residues
MRTIALLLAYDGTDFAGSQWQTDIRTVQGALESAWEALTQERRRIVLAGRTDAGVHATGQVAHVQTTTRHNLQTIWRGLN
AHLPVDLAIQNVGEAVPDFHARFSAIQREYRYLIDCAAAPLPQLRHQVLHYAGTLDVAAMTAALKLLEGTHDFAAFTTAT
PAQRSTVRTMYWTRVGDYEWFERRLLAIEVAANAFLQHMVRMIVGTLLLVGRGRMTVDQFGEVLASRDRRLAGPTAPAHG
LTLTAVRYPPGLIRWVEPSKRQNGTTKVEQPSSYVHEE

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family

Homologues:

Organism=Homo sapiens, GI23503259, Length=266, Percent_Identity=32.7067669172932, Blast_Score=87, Evalue=1e-17,
Organism=Escherichia coli, GI1788657, Length=249, Percent_Identity=40.9638554216867, Blast_Score=162, Evalue=2e-41,
Organism=Drosophila melanogaster, GI116007752, Length=302, Percent_Identity=28.476821192053, Blast_Score=74, Evalue=9e-14,
Organism=Drosophila melanogaster, GI24648455, Length=267, Percent_Identity=25.8426966292135, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI281362146, Length=267, Percent_Identity=25.8426966292135, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUA_CHLAA (A9WH95)

Other databases:

- EMBL:   CP000909
- RefSeq:   YP_001635996.1
- ProteinModelPortal:   A9WH95
- SMR:   A9WH95
- GeneID:   5826862
- GenomeReviews:   CP000909_GR
- KEGG:   cau:Caur_2398
- HOGENOM:   HBG621020
- OMA:   HSAIRRH
- ProtClustDB:   PRK14588
- HAMAP:   MF_00171
- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094
- Gene3D:   G3DSA:3.30.70.580
- Gene3D:   G3DSA:3.30.70.660
- PANTHER:   PTHR11142
- PIRSF:   PIRSF001430
- TIGRFAMs:   TIGR00071

Pfam domain/function: PF01416 PseudoU_synth_1; SSF55120 PsdUridine_synth_cat_dom

EC number: =5.4.99.12

Molecular weight: Translated: 31004; Mature: 31004

Theoretical pI: Translated: 9.34; Mature: 9.34

Prosite motif: NA

Important sites: ACT_SITE 52-52

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTIALLLAYDGTDFAGSQWQTDIRTVQGALESAWEALTQERRRIVLAGRTDAGVHATGQ
CCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCC
VAHVQTTTRHNLQTIWRGLNAHLPVDLAIQNVGEAVPDFHARFSAIQREYRYLIDCAAAP
EEEEEHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCC
LPQLRHQVLHYAGTLDVAAMTAALKLLEGTHDFAAFTTATPAQRSTVRTMYWTRVGDYEW
HHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCHHHHEECCCCCHHHHHHHHHHEECCCHHH
FERRLLAIEVAANAFLQHMVRMIVGTLLLVGRGRMTVDQFGEVLASRDRRLAGPTAPAHG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCC
LTLTAVRYPPGLIRWVEPSKRQNGTTKVEQPSSYVHEE
CEEEEEECCCCEEEECCCCCCCCCCCCCCCCCHHCCCC
>Mature Secondary Structure
MRTIALLLAYDGTDFAGSQWQTDIRTVQGALESAWEALTQERRRIVLAGRTDAGVHATGQ
CCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCC
VAHVQTTTRHNLQTIWRGLNAHLPVDLAIQNVGEAVPDFHARFSAIQREYRYLIDCAAAP
EEEEEHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCC
LPQLRHQVLHYAGTLDVAAMTAALKLLEGTHDFAAFTTATPAQRSTVRTMYWTRVGDYEW
HHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCHHHHEECCCCCHHHHHHHHHHEECCCHHH
FERRLLAIEVAANAFLQHMVRMIVGTLLLVGRGRMTVDQFGEVLASRDRRLAGPTAPAHG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCC
LTLTAVRYPPGLIRWVEPSKRQNGTTKVEQPSSYVHEE
CEEEEEECCCCEEEECCCCCCCCCCCCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA