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The map label for this gene is nfi [H]

Identifier: 222525777

GI number: 222525777

Start: 3179746

End: 3180438

Strand: Reverse

Name: nfi [H]

Synonym: Chy400_2530

Alternate gene names: 222525777

Gene position: 3180438-3179746 (Counterclockwise)

Preceding gene: 222525778

Following gene: 222525776

Centisome position: 60.36

GC content: 60.32

Gene sequence:

>693_bases
ATGCCGACGATTGATACCAGTGATGAGCGAGTCGCCGCAGCCATCGCCGAGCAGCAGGAACTCCGTGCCCGTGTACGCTT
GCACGACGACTACAGCGAATTACAGCTCGTTGCCGGGGTCGATGCCGGTTTTGAAGCCGATGGTACGCTCATTCGGGCGG
CGGTTGTGGTTGTGCGGTTACCCGATCTGGCACCGGTTGATTATGCATTGGTGCGGCAACCGGTGACCTTTCCCTACGTG
CCCGGTCTGCTTGCTTTTCGCGAGGCACCGGCAATTATCGCTGCGATCAGACAATTGCGGACGACGCCTGATCTGATCAT
CTGCGATGGTCACGGCATTGCCCATCCGCGCCGGTGCGGCATTGCCTGTCATATCGGTGTGCTCCTCGATCTACCGGCAA
TCGGTTGCGCAAAGCAACGTCTGATCGGGCGATTTACCGAACCCTTGCCTGAACGCGGTGATCGCAGTCCGTTGTACGAT
GGCGATGATCTGATTGGATGGGTGGTACGCACCCGCGCTACTGCCCGACCGGTCTTCATCTCTCCCGGTCATCGAGTCAG
CCTTGAGCGCGCCCCCGATCTGGTGATGGCCTGTACCACCAGATTTCGCCTGCCCGAACCGACGCGCCTGGCCCATCGCC
TGGCTTCGCACGACGACGCACCGCTCACCTCAGATTCTCCGCCACTGTTGTAA

Upstream 100 bases:

>100_bases
AAATGGCTGCGGGGGATTGAATTTCTCGACCATGATCGGCTGGGATTCTGGGAGCGTTACGGCTACAACAACAACGCTGA
TCCCTGGCGTGAAGAACGGT

Downstream 100 bases:

>100_bases
AAAAGTCGAAAAAAACCGCGTACCTTTTCGCGGGTTTGCACGGTTTCTATAGTAGGAAGCAAACCAACCGGAGGGAAACC
GTGCGCTGGTTTATCATTGG

Product: deoxyribonuclease V

Products: NA

Alternate protein names: Deoxyinosine 3'endonuclease; Deoxyribonuclease V; DNase V [H]

Number of amino acids: Translated: 230; Mature: 229

Protein sequence:

>230_residues
MPTIDTSDERVAAAIAEQQELRARVRLHDDYSELQLVAGVDAGFEADGTLIRAAVVVVRLPDLAPVDYALVRQPVTFPYV
PGLLAFREAPAIIAAIRQLRTTPDLIICDGHGIAHPRRCGIACHIGVLLDLPAIGCAKQRLIGRFTEPLPERGDRSPLYD
GDDLIGWVVRTRATARPVFISPGHRVSLERAPDLVMACTTRFRLPEPTRLAHRLASHDDAPLTSDSPPLL

Sequences:

>Translated_230_residues
MPTIDTSDERVAAAIAEQQELRARVRLHDDYSELQLVAGVDAGFEADGTLIRAAVVVVRLPDLAPVDYALVRQPVTFPYV
PGLLAFREAPAIIAAIRQLRTTPDLIICDGHGIAHPRRCGIACHIGVLLDLPAIGCAKQRLIGRFTEPLPERGDRSPLYD
GDDLIGWVVRTRATARPVFISPGHRVSLERAPDLVMACTTRFRLPEPTRLAHRLASHDDAPLTSDSPPLL
>Mature_229_residues
PTIDTSDERVAAAIAEQQELRARVRLHDDYSELQLVAGVDAGFEADGTLIRAAVVVVRLPDLAPVDYALVRQPVTFPYVP
GLLAFREAPAIIAAIRQLRTTPDLIICDGHGIAHPRRCGIACHIGVLLDLPAIGCAKQRLIGRFTEPLPERGDRSPLYDG
DDLIGWVVRTRATARPVFISPGHRVSLERAPDLVMACTTRFRLPEPTRLAHRLASHDDAPLTSDSPPLL

Specific function: Selectively cleaves double-stranded DNA at the second phosphodiester bond 3' to a deoxyinosine leaving behind the intact lesion on the nicked DNA. Acts in DNA repair [H]

COG id: COG1515

COG function: function code L; Deoxyinosine 3'endonuclease (endonuclease V)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the endonuclease V family [H]

Homologues:

Organism=Homo sapiens, GI31542775, Length=230, Percent_Identity=37.8260869565217, Blast_Score=122, Evalue=3e-28,
Organism=Homo sapiens, GI257467548, Length=225, Percent_Identity=31.5555555555556, Blast_Score=82, Evalue=3e-16,
Organism=Homo sapiens, GI257467550, Length=225, Percent_Identity=31.5555555555556, Blast_Score=81, Evalue=7e-16,
Organism=Escherichia coli, GI87082357, Length=210, Percent_Identity=47.1428571428571, Blast_Score=187, Evalue=5e-49,
Organism=Caenorhabditis elegans, GI71981509, Length=215, Percent_Identity=27.906976744186, Blast_Score=77, Evalue=9e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007581 [H]

Pfam domain/function: PF04493 Endonuclease_5 [H]

EC number: =3.1.21.7 [H]

Molecular weight: Translated: 25134; Mature: 25003

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTIDTSDERVAAAIAEQQELRARVRLHDDYSELQLVAGVDAGFEADGTLIRAAVVVVRL
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHEEEEECCCCCCCCCCHHHHHHHHHHCC
PDLAPVDYALVRQPVTFPYVPGLLAFREAPAIIAAIRQLRTTPDLIICDGHGIAHPRRCG
CCCCCCCHHHHCCCCCCCCCCHHHHHHHCHHHHHHHHHHCCCCCEEEECCCCCCCCCCCC
IACHIGVLLDLPAIGCAKQRLIGRFTEPLPERGDRSPLYDGDDLIGWVVRTRATARPVFI
CEEEEHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCEEE
SPGHRVSLERAPDLVMACTTRFRLPEPTRLAHRLASHDDAPLTSDSPPLL
CCCCEEECCCCCCCEEHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PTIDTSDERVAAAIAEQQELRARVRLHDDYSELQLVAGVDAGFEADGTLIRAAVVVVRL
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHEEEEECCCCCCCCCCHHHHHHHHHHCC
PDLAPVDYALVRQPVTFPYVPGLLAFREAPAIIAAIRQLRTTPDLIICDGHGIAHPRRCG
CCCCCCCHHHHCCCCCCCCCCHHHHHHHCHHHHHHHHHHCCCCCEEEECCCCCCCCCCCC
IACHIGVLLDLPAIGCAKQRLIGRFTEPLPERGDRSPLYDGDDLIGWVVRTRATARPVFI
CEEEEHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCEEE
SPGHRVSLERAPDLVMACTTRFRLPEPTRLAHRLASHDDAPLTSDSPPLL
CCCCEEECCCCCCCEEHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA