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The map label for this gene is cca [H]

Identifier: 222525752

GI number: 222525752

Start: 3146758

End: 3148260

Strand: Direct

Name: cca [H]

Synonym: Chy400_2505

Alternate gene names: 222525752

Gene position: 3146758-3148260 (Clockwise)

Preceding gene: 222525751

Following gene: 222525755

Centisome position: 59.72

GC content: 60.48

Gene sequence:

>1503_bases
TTGCCGACTAAGGACATCTGTGGCGACGTGATCCTGGTTCGTGTTATGGTGAATGACCAGCTTAACAACCTGTTCCAGCT
TATTACCGCTTGTACGTCGCGCCCGGTGTGGCTGGTCGGTGGTGCAGTCCGAGAGCTTTTAACCGGGCACCAGCCGGTTG
ATCTCGATCTCGCGATTGACGGGAGTGGCCTGGCGCTCGCGCAGGCGCTGGCCGATGCCGGCCAGGGCACCTTCGTGCTG
CTTGATCGCGAACGTGATACCGGTCGCGTCGTGCTGCCTGATAGCATCGTGATCGACTGCGCCCGCCTGCGGGCAACCGA
TATTGTGTCCGATCTTTACCTGCGCGACTTTACCGTAAATGCGCTGGCACTCCCGTTGGATGCAGCACGCTGGGGCGACT
GGAGTGCATTACTTGATCCACTCGACGGGCAGGCCGATCTTGCCGCACGCCGCTTGCGCCCGTGTACCCCGACCAGTCTA
CGCGATGACCCGTTGCGCGTGATTCGGGCCGGTCGCTTTCGCGCCACCCATCAGCTCATCCCTGTGCCCGAACTGGTGAC
CCCTGCGCGTGAGGTAGTCCCATTGCTATCAGCGATTGCCGTTGAACGCATCCGTGACGAATTGCTCAAGCTCTGCGATG
GGTTAGCTGCCGCTGCCGGTTTGCGCTTCCTGGATCAGGTCGGCGCACTCACCGCCATCTTCCCCGAACTGGAAGCGGCA
CGTACCTGCGATCAGCCGCACGTCCATTTTCTGCCGGTGTTGGCCCATATGCTGGAAACAGTTGCCGCGCTGGATTGGCT
GATTGACAACGGCGAGCCACCTGTTGCCGTCCAGACCCATCCCCACCTGGAACGCATACTCCCGTTTGCCGATCAGTATC
AGGCTCTTCTCCACCAACGGCGCGGCAATGTCCGGCGGGCAGCCCTCTTAAAACTGGCAGCCCTGCTCCACGATAACGCC
AAACCGCAGACGAAAGTCAGCCATCCCGATGGTACAGTAACCTTCTACGGCCATCAGGGGTTGGGTGCCGAGCGCGTTTT
GATCATCGCTCAGCGCTTACGGCTCAGTCGGGCCGATACCGCATACCTGGCCTGCATTGTTCGTGAACATATGCGTCCAG
GCCAAATGCGGAGTGGTGGTCAACTCACGGAACGGGGGATTGCCCGCTTCTTCCGTGATACCGGCGATGCCGGGCCTGAC
ATTCTCTTGCACGAACTGGCCGACCATCTGGCAACCCGTGGCCCCTGGCTTGACCCAATGGCGTGGCGGGAACATCTCCA
TTGGGTGGCAATGATGCTGAACCATTACTGGAACGCGCCGACCCCACCGCCGCCACCATTACTGCGTGGCGACACCCTAA
TGGCTGCGTTGGGGATCAAACCGGGGCCGGAAGTAGGTCGCCTGTTACGCTTAATCCACGAAGCGCAACTGGCGGGAGAG
ATTCATAGTCCTGAAGAGGCGATTGCGCTGGCTCGCACACTACAGACCACCGATCACGGATAA

Upstream 100 bases:

>100_bases
GCATCAGCCGGTTGATCTCGATCTCGCGATTGACGGGAGTGGCCTGGCTTATGGAGCCGCCCGGCGGTTGAACGCCATTC
GACACAGGTGCTGACCTTAC

Downstream 100 bases:

>100_bases
CGGATCATCAGCGTGGTCGGCGCGGCAAACGGATCTGGTCGGTCTGCTGCCGTTCACCGGCGGCAGGACGATCATCGTCA
AGGAGCGGCAACAGGATCAG

Product: polynucleotide adenylyltransferase

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT [H]

Number of amino acids: Translated: 500; Mature: 499

Protein sequence:

>500_residues
MPTKDICGDVILVRVMVNDQLNNLFQLITACTSRPVWLVGGAVRELLTGHQPVDLDLAIDGSGLALAQALADAGQGTFVL
LDRERDTGRVVLPDSIVIDCARLRATDIVSDLYLRDFTVNALALPLDAARWGDWSALLDPLDGQADLAARRLRPCTPTSL
RDDPLRVIRAGRFRATHQLIPVPELVTPAREVVPLLSAIAVERIRDELLKLCDGLAAAAGLRFLDQVGALTAIFPELEAA
RTCDQPHVHFLPVLAHMLETVAALDWLIDNGEPPVAVQTHPHLERILPFADQYQALLHQRRGNVRRAALLKLAALLHDNA
KPQTKVSHPDGTVTFYGHQGLGAERVLIIAQRLRLSRADTAYLACIVREHMRPGQMRSGGQLTERGIARFFRDTGDAGPD
ILLHELADHLATRGPWLDPMAWREHLHWVAMMLNHYWNAPTPPPPPLLRGDTLMAALGIKPGPEVGRLLRLIHEAQLAGE
IHSPEEAIALARTLQTTDHG

Sequences:

>Translated_500_residues
MPTKDICGDVILVRVMVNDQLNNLFQLITACTSRPVWLVGGAVRELLTGHQPVDLDLAIDGSGLALAQALADAGQGTFVL
LDRERDTGRVVLPDSIVIDCARLRATDIVSDLYLRDFTVNALALPLDAARWGDWSALLDPLDGQADLAARRLRPCTPTSL
RDDPLRVIRAGRFRATHQLIPVPELVTPAREVVPLLSAIAVERIRDELLKLCDGLAAAAGLRFLDQVGALTAIFPELEAA
RTCDQPHVHFLPVLAHMLETVAALDWLIDNGEPPVAVQTHPHLERILPFADQYQALLHQRRGNVRRAALLKLAALLHDNA
KPQTKVSHPDGTVTFYGHQGLGAERVLIIAQRLRLSRADTAYLACIVREHMRPGQMRSGGQLTERGIARFFRDTGDAGPD
ILLHELADHLATRGPWLDPMAWREHLHWVAMMLNHYWNAPTPPPPPLLRGDTLMAALGIKPGPEVGRLLRLIHEAQLAGE
IHSPEEAIALARTLQTTDHG
>Mature_499_residues
PTKDICGDVILVRVMVNDQLNNLFQLITACTSRPVWLVGGAVRELLTGHQPVDLDLAIDGSGLALAQALADAGQGTFVLL
DRERDTGRVVLPDSIVIDCARLRATDIVSDLYLRDFTVNALALPLDAARWGDWSALLDPLDGQADLAARRLRPCTPTSLR
DDPLRVIRAGRFRATHQLIPVPELVTPAREVVPLLSAIAVERIRDELLKLCDGLAAAAGLRFLDQVGALTAIFPELEAAR
TCDQPHVHFLPVLAHMLETVAALDWLIDNGEPPVAVQTHPHLERILPFADQYQALLHQRRGNVRRAALLKLAALLHDNAK
PQTKVSHPDGTVTFYGHQGLGAERVLIIAQRLRLSRADTAYLACIVREHMRPGQMRSGGQLTERGIARFFRDTGDAGPDI
LLHELADHLATRGPWLDPMAWREHLHWVAMMLNHYWNAPTPPPPPLLRGDTLMAALGIKPGPEVGRLLRLIHEAQLAGEI
HSPEEAIALARTLQTTDHG

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023068
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 54955; Mature: 54824

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTKDICGDVILVRVMVNDQLNNLFQLITACTSRPVWLVGGAVRELLTGHQPVDLDLAID
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHCCCCCCEEEEEEC
GSGLALAQALADAGQGTFVLLDRERDTGRVVLPDSIVIDCARLRATDIVSDLYLRDFTVN
CCCHHHHHHHHHCCCCEEEEEECCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHCCHH
ALALPLDAARWGDWSALLDPLDGQADLAARRLRPCTPTSLRDDPLRVIRAGRFRATHQLI
HEEECCCCCCCCCHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCHHHCCCC
PVPELVTPAREVVPLLSAIAVERIRDELLKLCDGLAAAAGLRFLDQVGALTAIFPELEAA
CCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RTCDQPHVHFLPVLAHMLETVAALDWLIDNGEPPVAVQTHPHLERILPFADQYQALLHQR
HCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCHHHHHHCCHHHHHHHHHHHH
RGNVRRAALLKLAALLHDNAKPQTKVSHPDGTVTFYGHQGLGAERVLIIAQRLRLSRADT
CCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCHH
AYLACIVREHMRPGQMRSGGQLTERGIARFFRDTGDAGPDILLHELADHLATRGPWLDPM
HHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCHH
AWREHLHWVAMMLNHYWNAPTPPPPPLLRGDTLMAALGIKPGPEVGRLLRLIHEAQLAGE
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHCC
IHSPEEAIALARTLQTTDHG
CCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PTKDICGDVILVRVMVNDQLNNLFQLITACTSRPVWLVGGAVRELLTGHQPVDLDLAID
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHCCCCCCEEEEEEC
GSGLALAQALADAGQGTFVLLDRERDTGRVVLPDSIVIDCARLRATDIVSDLYLRDFTVN
CCCHHHHHHHHHCCCCEEEEEECCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHCCHH
ALALPLDAARWGDWSALLDPLDGQADLAARRLRPCTPTSLRDDPLRVIRAGRFRATHQLI
HEEECCCCCCCCCHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCHHHCCCC
PVPELVTPAREVVPLLSAIAVERIRDELLKLCDGLAAAAGLRFLDQVGALTAIFPELEAA
CCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RTCDQPHVHFLPVLAHMLETVAALDWLIDNGEPPVAVQTHPHLERILPFADQYQALLHQR
HCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCHHHHHHCCHHHHHHHHHHHH
RGNVRRAALLKLAALLHDNAKPQTKVSHPDGTVTFYGHQGLGAERVLIIAQRLRLSRADT
CCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCHH
AYLACIVREHMRPGQMRSGGQLTERGIARFFRDTGDAGPDILLHELADHLATRGPWLDPM
HHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCHH
AWREHLHWVAMMLNHYWNAPTPPPPPLLRGDTLMAALGIKPGPEVGRLLRLIHEAQLAGE
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHCC
IHSPEEAIALARTLQTTDHG
CCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]