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The map label for this gene is mrpD [H]

Identifier: 222525637

GI number: 222525637

Start: 3015685

End: 3017202

Strand: Direct

Name: mrpD [H]

Synonym: Chy400_2388

Alternate gene names: 222525637

Gene position: 3015685-3017202 (Clockwise)

Preceding gene: 222525636

Following gene: 222525638

Centisome position: 57.24

GC content: 54.48

Gene sequence:

>1518_bases
ATGGTCTTTCATCTCTTCGTTCCAATAGTCTCACCATTGATCGGTGCTGCTCTGGCCGTTCTGTTTGGTCATCAGCGTAT
GATTGCACGTGCAATTACGCTGGCGAGTGTGCTCATTAATCTCGGCTATGGTCTCTGGCTGATGAGCGTGGTACCTCAGA
GTGGCCCACTGGTCGCCCAGGCGTCGGGCTGGCTGGCACCGTTTGGTATTTCGCTGGTTGCCGATGGCATGAGCGCGTTG
ATGGTCATGCTGGCCGCGCTCCTCATGCTGACGACGGTTGTCTATAGCTTTTACTCGATTGATCGCGAGCGTGAACGCTA
TTTCTACTACCCACTGTTGCTGTTATTGCTGCTAGGGGTGAGTGGTGCGTTTCTTACCGGTGATCTCTTCAACCTGTACG
TTTGGTTTGAAGTCTTGCTGGTTTCCTCATTTGGTTTGATTACCCTCGGTGGTAGTCGCGATCAGCTCGAAGGTGGCTTG
AAATACGTTGTGCTCAACCTGCTCGGCAGTACTGCCTTCTTGTTTGGTTGTGGCCTGATGTACGGCTTTGCCGGCACGTT
AAATATGGCGCACATCGCCGAGCGTATGGCTACATTGACCAATCCCGGTTTGCAGACTGTGCTGGCCGGCGTTTTCCTCT
TCGCATTCGGTAGCAAAGCGGCGATTGTGCCTCTCTTCTTCTGGTTGCCATCGAGCTACCACACGCCACCGGTAGCGGTA
ACCGCAATCTTCAGCGGTTTGATGACAAAGGTGGGTGTCTATTCGCTATACCGGGTCTTTGGGCTGATGCTGCAAAACGA
GCTGGCGATCTACGCGCCGTGGATTACGCTGCTGGCCGGGTTGACGATGGTGATAGGTGTGTTGGGTGCGGTTGCCCAGA
TGAATGTGCGGCGTATTCTCAGCTTCCACATCATCAGTCAGATCGGGTATAGCGTGATGGGGTTGGGATTAGCCAGTGTA
GCCGGGCTGGCTGCCGGTCTGGTCTTCACCGCCCATGTGATGATTGTGAAGATGGCGCTCTTCTTTATCGGTGGCGCTGC
TGAACAGATTTACGGTACCGGTGATCTGAAAAAGATGGGTGGGTTGGCCCGGCGTGAACCGTGGCTGGCAATCTTCTGGT
TCGTCGGTATTCTGTCGCTGGCCGGCATTCCACCGCTGAGTGGGTTCATCGGCAAGCTGATCTTGCTCCAGGTCGGCGTG
AGTCAGCAGGCGTATCTGATTACAGCAGTGGCTGCCGGTACCAGTATCCTCACCTTCTTCTCGATGTTGAAGATTTGGAA
TGAGGTCTTCTGGAAGAAATCCTACGAGGACGTGAATCGGCTGCCACGGGTTAGTTTCGGCTTGCTGGCGCCAGGGGCTG
CTCTGGTGATTTTGAGCATCGCGCTTGGGCTACTGGCCGGGCCGCTGGTCGATTACAATACTATCGCCGCGCAACAGTTT
TTTGATCGGACAATCTACATTACGGCTGTCTGTGGTAGTGATGGCTGTGAAGCGGTATATCAGGCGGCTATGCGGTAG

Upstream 100 bases:

>100_bases
TCTGGCCTATCGCTCGAATCAGGCGGTGGGGAGCGACGATCTCGATGATCTGTCAACAACCGATAAGTTATAGTGTTGCG
ATCTGGCGTAGGAAAGAAGC

Downstream 100 bases:

>100_bases
AGGTGATTATGATTGTATTGATCCTTTTGCTGACAATCACCTGGATGGCATTGCAGAGCAGTTTTACCCTGCCCGATTTG
ATCGTCGGTTTCCTGGTCAG

Product: NADH dehydrogenase (quinone)

Products: H2; oxidized ferredoxin [C]

Alternate protein names: Mrp complex subunit D; Multiple resistance and pH homeostasis protein D [H]

Number of amino acids: Translated: 505; Mature: 505

Protein sequence:

>505_residues
MVFHLFVPIVSPLIGAALAVLFGHQRMIARAITLASVLINLGYGLWLMSVVPQSGPLVAQASGWLAPFGISLVADGMSAL
MVMLAALLMLTTVVYSFYSIDRERERYFYYPLLLLLLLGVSGAFLTGDLFNLYVWFEVLLVSSFGLITLGGSRDQLEGGL
KYVVLNLLGSTAFLFGCGLMYGFAGTLNMAHIAERMATLTNPGLQTVLAGVFLFAFGSKAAIVPLFFWLPSSYHTPPVAV
TAIFSGLMTKVGVYSLYRVFGLMLQNELAIYAPWITLLAGLTMVIGVLGAVAQMNVRRILSFHIISQIGYSVMGLGLASV
AGLAAGLVFTAHVMIVKMALFFIGGAAEQIYGTGDLKKMGGLARREPWLAIFWFVGILSLAGIPPLSGFIGKLILLQVGV
SQQAYLITAVAAGTSILTFFSMLKIWNEVFWKKSYEDVNRLPRVSFGLLAPGAALVILSIALGLLAGPLVDYNTIAAQQF
FDRTIYITAVCGSDGCEAVYQAAMR

Sequences:

>Translated_505_residues
MVFHLFVPIVSPLIGAALAVLFGHQRMIARAITLASVLINLGYGLWLMSVVPQSGPLVAQASGWLAPFGISLVADGMSAL
MVMLAALLMLTTVVYSFYSIDRERERYFYYPLLLLLLLGVSGAFLTGDLFNLYVWFEVLLVSSFGLITLGGSRDQLEGGL
KYVVLNLLGSTAFLFGCGLMYGFAGTLNMAHIAERMATLTNPGLQTVLAGVFLFAFGSKAAIVPLFFWLPSSYHTPPVAV
TAIFSGLMTKVGVYSLYRVFGLMLQNELAIYAPWITLLAGLTMVIGVLGAVAQMNVRRILSFHIISQIGYSVMGLGLASV
AGLAAGLVFTAHVMIVKMALFFIGGAAEQIYGTGDLKKMGGLARREPWLAIFWFVGILSLAGIPPLSGFIGKLILLQVGV
SQQAYLITAVAAGTSILTFFSMLKIWNEVFWKKSYEDVNRLPRVSFGLLAPGAALVILSIALGLLAGPLVDYNTIAAQQF
FDRTIYITAVCGSDGCEAVYQAAMR
>Mature_505_residues
MVFHLFVPIVSPLIGAALAVLFGHQRMIARAITLASVLINLGYGLWLMSVVPQSGPLVAQASGWLAPFGISLVADGMSAL
MVMLAALLMLTTVVYSFYSIDRERERYFYYPLLLLLLLGVSGAFLTGDLFNLYVWFEVLLVSSFGLITLGGSRDQLEGGL
KYVVLNLLGSTAFLFGCGLMYGFAGTLNMAHIAERMATLTNPGLQTVLAGVFLFAFGSKAAIVPLFFWLPSSYHTPPVAV
TAIFSGLMTKVGVYSLYRVFGLMLQNELAIYAPWITLLAGLTMVIGVLGAVAQMNVRRILSFHIISQIGYSVMGLGLASV
AGLAAGLVFTAHVMIVKMALFFIGGAAEQIYGTGDLKKMGGLARREPWLAIFWFVGILSLAGIPPLSGFIGKLILLQVGV
SQQAYLITAVAAGTSILTFFSMLKIWNEVFWKKSYEDVNRLPRVSFGLLAPGAALVILSIALGLLAGPLVDYNTIAAQQF
FDRTIYITAVCGSDGCEAVYQAAMR

Specific function: Mrp complex is a Na(+)/H(+) antiporter involved in Na(+) excretion and Na(+) resistance [H]

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CPA3 antiporters (TC 2.A.63) subunit D family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=327, Percent_Identity=24.1590214067278, Blast_Score=72, Evalue=8e-13,
Organism=Escherichia coli, GI1788827, Length=280, Percent_Identity=29.2857142857143, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1788613, Length=446, Percent_Identity=28.0269058295964, Blast_Score=106, Evalue=4e-24,
Organism=Escherichia coli, GI1788831, Length=413, Percent_Identity=24.455205811138, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1788829, Length=401, Percent_Identity=27.1820448877805, Blast_Score=93, Evalue=5e-20,
Organism=Escherichia coli, GI145693160, Length=364, Percent_Identity=27.4725274725275, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI2367154, Length=316, Percent_Identity=27.2151898734177, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI1788614, Length=331, Percent_Identity=24.4712990936556, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR003918 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 54309; Mature: 54309

Theoretical pI: Translated: 9.23; Mature: 9.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVFHLFVPIVSPLIGAALAVLFGHQRMIARAITLASVLINLGYGLWLMSVVPQSGPLVAQ
CEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
ASGWLAPFGISLVADGMSALMVMLAALLMLTTVVYSFYSIDRERERYFYYPLLLLLLLGV
CCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCC
SGAFLTGDLFNLYVWFEVLLVSSFGLITLGGSRDQLEGGLKYVVLNLLGSTAFLFGCGLM
CCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHCCHHHHHHHHHHHHHHHHHHHHH
YGFAGTLNMAHIAERMATLTNPGLQTVLAGVFLFAFGSKAAIVPLFFWLPSSYHTPPVAV
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHH
TAIFSGLMTKVGVYSLYRVFGLMLQNELAIYAPWITLLAGLTMVIGVLGAVAQMNVRRIL
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SFHIISQIGYSVMGLGLASVAGLAAGLVFTAHVMIVKMALFFIGGAAEQIYGTGDLKKMG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCHHHHC
GLARREPWLAIFWFVGILSLAGIPPLSGFIGKLILLQVGVSQQAYLITAVAAGTSILTFF
CCHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
SMLKIWNEVFWKKSYEDVNRLPRVSFGLLAPGAALVILSIALGLLAGPLVDYNTIAAQQF
HHHHHHHHHHHCCCHHHHHCCCCCHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
FDRTIYITAVCGSDGCEAVYQAAMR
HCCEEEEEEEECCCCHHHHHHHHCC
>Mature Secondary Structure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HCCEEEEEEEECCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton; reduced ferredoxin [C]

Specific reaction: Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11356194 [H]