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The map label for this gene is walK [H]

Identifier: 222525510

GI number: 222525510

Start: 2858763

End: 2859953

Strand: Reverse

Name: walK [H]

Synonym: Chy400_2258

Alternate gene names: 222525510

Gene position: 2859953-2858763 (Counterclockwise)

Preceding gene: 222525511

Following gene: 222525506

Centisome position: 54.28

GC content: 59.11

Gene sequence:

>1191_bases
ATGAACCAGTTGCCACAAATTCTGTACATTGAAGACAACCCAGACAATCAGCGTCTGGTTCAGCGAGTACTCAGCGCTCG
TGGCTATCAGGTCGTCATCGCCGAAGACGGCCCTGGTGGTCTCGCGCTTGCCCGCACGACCCATCCGGCGCTAGTGCTGG
TCGATCTCGGCATCCCTGGTCTCGATGGCTACGAGACGACAACCCGCCTGCGTTCGCTGGCCCACATGCACAATGTGCCG
ATTATCGCCCTGACCGCAGATGCCTCACCCGGATCACGCGAACGTGCCTTAGTTGCCGGCTGCGATGGGTATCTGAGTAA
GCCGATTGACGCCCGTGCGCTGCCGGCGCAGATCGCCGAATTCATTGAGGGGCGTCGCGAACGCTTGCCGGAAGTCATGG
AGACGGAGCTGTTACGCTCGTACAACCAGAAGCTGGTCGAGCGGCTTGAGGCACGGGTACGTGAACTGACACTGGCCAAC
GCCCAATTGCAAGACCTCGACCGGATGAAGGATCAGTTCCTGGCTACCCTCTCGCACGAACTCCGCACTCCCCTCACGTC
GCTCCTTGGTTATCTCGAACTCTTCGACCGCGGCATGCTGGGTCCGCTCACACCACAGCAGGAAGAAGCGGTTCGCGTCA
TGCGGCGTAGCAGCGAAGTGCTGGCCCGCCAGCTCAACAACCTGCTCTACTTTCAAGAGGTACGCAGCCGATCACTCAAT
TTCGTGCAATTACAGCCGCTCGATATTCTGCGCCCACTTATCGCCGCCGCTCGCGAACGGGCAGCCCAGGCGCAGTTGCA
GTTCGAGGTCCTCCTTTCAGAAATGCAACCTATTGAGGTTGACCGCTCGGCATTTGAACATCTGGTACGCAATCTCCTCG
ACAACGCCTTCCAATACACCGAACCCGGTGGGCGAGTGCGGTTCATTGTCCGTGATGAACCAACCCGCCTGATCATTCGG
GTCGAAGATACCGGGATCGGCATTCCTGCCGGCGAGCAAGAAAAAATCTTCCTCCCCTTTTACCAGGTTGATCATTCGCT
GGCTCGTGGTCATCCCGGCAGTGGACTTGGGCTGGCGATTGTACGCCATATCGTCGAAGCGCACGGTGGTCAGATTGCGC
TGCGGAGCGCCCCTGGCGAGGGAAGTGCGTTTACGATAGCTATTCCGCGCCGACAGGTAGCCGGTGGGTGA

Upstream 100 bases:

>100_bases
TTGATAGTACACCAGGGATTGGTTCAACATTTACCTTCACGATACCGGTTGCGCAACCGGCATTGCTGGCAGCGCCGGTA
AACATTGACACAACGACGCC

Downstream 100 bases:

>100_bases
TTGATCACCACAAAGACACAGAGGGCACAGAGGGTTTTGTAAGAGTCTCTGTGTCCTCTGTGCCTCGGTGGTGAATACAT
ACCAGGCTGTGTCGACTATC

Product: response regulator receiver sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 396; Mature: 396

Protein sequence:

>396_residues
MNQLPQILYIEDNPDNQRLVQRVLSARGYQVVIAEDGPGGLALARTTHPALVLVDLGIPGLDGYETTTRLRSLAHMHNVP
IIALTADASPGSRERALVAGCDGYLSKPIDARALPAQIAEFIEGRRERLPEVMETELLRSYNQKLVERLEARVRELTLAN
AQLQDLDRMKDQFLATLSHELRTPLTSLLGYLELFDRGMLGPLTPQQEEAVRVMRRSSEVLARQLNNLLYFQEVRSRSLN
FVQLQPLDILRPLIAAARERAAQAQLQFEVLLSEMQPIEVDRSAFEHLVRNLLDNAFQYTEPGGRVRFIVRDEPTRLIIR
VEDTGIGIPAGEQEKIFLPFYQVDHSLARGHPGSGLGLAIVRHIVEAHGGQIALRSAPGEGSAFTIAIPRRQVAGG

Sequences:

>Translated_396_residues
MNQLPQILYIEDNPDNQRLVQRVLSARGYQVVIAEDGPGGLALARTTHPALVLVDLGIPGLDGYETTTRLRSLAHMHNVP
IIALTADASPGSRERALVAGCDGYLSKPIDARALPAQIAEFIEGRRERLPEVMETELLRSYNQKLVERLEARVRELTLAN
AQLQDLDRMKDQFLATLSHELRTPLTSLLGYLELFDRGMLGPLTPQQEEAVRVMRRSSEVLARQLNNLLYFQEVRSRSLN
FVQLQPLDILRPLIAAARERAAQAQLQFEVLLSEMQPIEVDRSAFEHLVRNLLDNAFQYTEPGGRVRFIVRDEPTRLIIR
VEDTGIGIPAGEQEKIFLPFYQVDHSLARGHPGSGLGLAIVRHIVEAHGGQIALRSAPGEGSAFTIAIPRRQVAGG
>Mature_396_residues
MNQLPQILYIEDNPDNQRLVQRVLSARGYQVVIAEDGPGGLALARTTHPALVLVDLGIPGLDGYETTTRLRSLAHMHNVP
IIALTADASPGSRERALVAGCDGYLSKPIDARALPAQIAEFIEGRRERLPEVMETELLRSYNQKLVERLEARVRELTLAN
AQLQDLDRMKDQFLATLSHELRTPLTSLLGYLELFDRGMLGPLTPQQEEAVRVMRRSSEVLARQLNNLLYFQEVRSRSLN
FVQLQPLDILRPLIAAARERAAQAQLQFEVLLSEMQPIEVDRSAFEHLVRNLLDNAFQYTEPGGRVRFIVRDEPTRLIIR
VEDTGIGIPAGEQEKIFLPFYQVDHSLARGHPGSGLGLAIVRHIVEAHGGQIALRSAPGEGSAFTIAIPRRQVAGG

Specific function: Member of the two-component regulatory system walK/walR that regulates genes involved in autolysis, biofilm formation and cell wall metabolism. WalK functions as a sensor protein kinase which is autophosphorylated at a histidine residue and transfers its

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI145693157, Length=246, Percent_Identity=33.3333333333333, Blast_Score=125, Evalue=4e-30,
Organism=Escherichia coli, GI1789149, Length=255, Percent_Identity=31.7647058823529, Blast_Score=121, Evalue=1e-28,
Organism=Escherichia coli, GI48994928, Length=240, Percent_Identity=35.4166666666667, Blast_Score=111, Evalue=8e-26,
Organism=Escherichia coli, GI1788393, Length=235, Percent_Identity=33.1914893617021, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1788713, Length=244, Percent_Identity=31.5573770491803, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI1788549, Length=220, Percent_Identity=30, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI87082128, Length=235, Percent_Identity=31.063829787234, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1786600, Length=238, Percent_Identity=29.8319327731092, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1786912, Length=228, Percent_Identity=31.5789473684211, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI87081816, Length=271, Percent_Identity=29.520295202952, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1790436, Length=234, Percent_Identity=28.2051282051282, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI87082012, Length=113, Percent_Identity=38.0530973451327, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1790346, Length=253, Percent_Identity=29.2490118577075, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1786783, Length=264, Percent_Identity=28.030303030303, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1786837, Length=306, Percent_Identity=28.1045751633987, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788279, Length=249, Percent_Identity=29.3172690763052, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1790861, Length=213, Percent_Identity=28.169014084507, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1787894, Length=224, Percent_Identity=29.9107142857143, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1789808, Length=257, Percent_Identity=28.0155642023346, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1786599, Length=113, Percent_Identity=31.858407079646, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1786911, Length=115, Percent_Identity=35.6521739130435, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1788394, Length=179, Percent_Identity=29.608938547486, Blast_Score=62, Evalue=8e-11,
Organism=Saccharomyces cerevisiae, GI6322044, Length=93, Percent_Identity=41.9354838709677, Blast_Score=69, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 44093; Mature: 44093

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQLPQILYIEDNPDNQRLVQRVLSARGYQVVIAEDGPGGLALARTTHPALVLVDLGIPG
CCCCCCEEEEECCCCHHHHHHHHHHCCCCEEEEEECCCCCEEEEECCCCEEEEEECCCCC
LDGYETTTRLRSLAHMHNVPIIALTADASPGSRERALVAGCDGYLSKPIDARALPAQIAE
CCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCEEEECCCHHHCCCCCCCCCHHHHHH
FIEGRRERLPEVMETELLRSYNQKLVERLEARVRELTLANAQLQDLDRMKDQFLATLSHE
HHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
LRTPLTSLLGYLELFDRGMLGPLTPQQEEAVRVMRRSSEVLARQLNNLLYFQEVRSRSLN
HHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
FVQLQPLDILRPLIAAARERAAQAQLQFEVLLSEMQPIEVDRSAFEHLVRNLLDNAFQYT
EEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCC
EPGGRVRFIVRDEPTRLIIRVEDTGIGIPAGEQEKIFLPFYQVDHSLARGHPGSGLGLAI
CCCCEEEEEEECCCCEEEEEEECCCCCCCCCCCCEEEEHHHHHHHHHHCCCCCCCHHHHH
VRHIVEAHGGQIALRSAPGEGSAFTIAIPRRQVAGG
HHHHHHHCCCEEEEECCCCCCCEEEEEECCHHCCCC
>Mature Secondary Structure
MNQLPQILYIEDNPDNQRLVQRVLSARGYQVVIAEDGPGGLALARTTHPALVLVDLGIPG
CCCCCCEEEEECCCCHHHHHHHHHHCCCCEEEEEECCCCCEEEEECCCCEEEEEECCCCC
LDGYETTTRLRSLAHMHNVPIIALTADASPGSRERALVAGCDGYLSKPIDARALPAQIAE
CCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCEEEECCCHHHCCCCCCCCCHHHHHH
FIEGRRERLPEVMETELLRSYNQKLVERLEARVRELTLANAQLQDLDRMKDQFLATLSHE
HHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
LRTPLTSLLGYLELFDRGMLGPLTPQQEEAVRVMRRSSEVLARQLNNLLYFQEVRSRSLN
HHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
FVQLQPLDILRPLIAAARERAAQAQLQFEVLLSEMQPIEVDRSAFEHLVRNLLDNAFQYT
EEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCC
EPGGRVRFIVRDEPTRLIIRVEDTGIGIPAGEQEKIFLPFYQVDHSLARGHPGSGLGLAI
CCCCEEEEEEECCCCEEEEEEECCCCCCCCCCCCEEEEHHHHHHHHHHCCCCCCCHHHHH
VRHIVEAHGGQIALRSAPGEGSAFTIAIPRRQVAGG
HHHHHHHCCCEEEEECCCCCCCEEEEEECCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA