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The map label for this gene is yacO [H]

Identifier: 222525486

GI number: 222525486

Start: 2825462

End: 2826220

Strand: Reverse

Name: yacO [H]

Synonym: Chy400_2234

Alternate gene names: 222525486

Gene position: 2826220-2825462 (Counterclockwise)

Preceding gene: 222525487

Following gene: 222525485

Centisome position: 53.64

GC content: 60.34

Gene sequence:

>759_bases
ATGGCAACCGAGTTGCTATACGGGCGCAACGCGGTACGCGAGTCTCTTCGCGCCCGCCGTCGCGTATTCCACCGTTTGTA
TGTCTCGCAAAGTGTGCAGGAAAGCGGTATTATCGCTGAAATTATCAATCTGGCCCAGCAGGCCGGGGTGCCGGTTGAAC
GCATCGAACGCCAGACCCTCGACCGCCGTCTGCGTGATGCCAATCATCAAGGAGTCATGCTAGAGGCTGAGCCGTACCCT
TACGTTGAACTCGACACCTGTCTCAATGCCGCTGCCGAACGCCGCGAGGCAGCGTTACTCTTGTTGCTCGACCATCTGCA
AGACCCGCAGAATATCGGTACCCTGCTCCGCACCGCCGAAGCGGTAGGATGCCACGGCGTGGTCATCCCCGGTCGGCGTG
CCGCCGAGATCACACCGGCAGTCGTTAACGCCAGCAGTGGCGCCGTAGAGCACCTGCGCGTCGCTATGGTGACAAACCTC
AATCAGACCATCGAAGAGTTGCAGCACGCCGGCATTTGGGTGGTCGGTCTCGAACAAGATGAACAGGCCCAGGATATTGA
TGCCGTTGACCTCGATCTCCCGCTGGCCCTGGTCATCGGCGCGGAAGGCACCGGTATCGCCCGCCTGGTACGGGAACGGT
GCGACTTTTTAGTCCGCTTGCCGATTGTCGGCCAGGTTGGATCACTCAATGCGGCTATTGCCGGCAGCATCGCCATCTAT
CACGCCTGGCGACAGCGCCATCGCCCGGTTACCGATTAG

Upstream 100 bases:

>100_bases
CATCACGATGAAGGATTTTTCGGGCGCATCAAAGATGCCTTTGGTCTGTAGACCCTTGCCGGTGTGGTAAACACCGGCAC
ATAGCTTGAGAGAGTTGATT

Downstream 100 bases:

>100_bases
ATTAGATGGTGATCACTACAGCGTAGTACCGACTTCAGCCTGTGCCACGTATGCGGCAAAACCAGCGCACGGATGGCAAG
AGAGGAATCTACTCGTCCAT

Product: TrmH family RNA methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 252; Mature: 251

Protein sequence:

>252_residues
MATELLYGRNAVRESLRARRRVFHRLYVSQSVQESGIIAEIINLAQQAGVPVERIERQTLDRRLRDANHQGVMLEAEPYP
YVELDTCLNAAAERREAALLLLLDHLQDPQNIGTLLRTAEAVGCHGVVIPGRRAAEITPAVVNASSGAVEHLRVAMVTNL
NQTIEELQHAGIWVVGLEQDEQAQDIDAVDLDLPLALVIGAEGTGIARLVRERCDFLVRLPIVGQVGSLNAAIAGSIAIY
HAWRQRHRPVTD

Sequences:

>Translated_252_residues
MATELLYGRNAVRESLRARRRVFHRLYVSQSVQESGIIAEIINLAQQAGVPVERIERQTLDRRLRDANHQGVMLEAEPYP
YVELDTCLNAAAERREAALLLLLDHLQDPQNIGTLLRTAEAVGCHGVVIPGRRAAEITPAVVNASSGAVEHLRVAMVTNL
NQTIEELQHAGIWVVGLEQDEQAQDIDAVDLDLPLALVIGAEGTGIARLVRERCDFLVRLPIVGQVGSLNAAIAGSIAIY
HAWRQRHRPVTD
>Mature_251_residues
ATELLYGRNAVRESLRARRRVFHRLYVSQSVQESGIIAEIINLAQQAGVPVERIERQTLDRRLRDANHQGVMLEAEPYPY
VELDTCLNAAAERREAALLLLLDHLQDPQNIGTLLRTAEAVGCHGVVIPGRRAAEITPAVVNASSGAVEHLRVAMVTNLN
QTIEELQHAGIWVVGLEQDEQAQDIDAVDLDLPLALVIGAEGTGIARLVRERCDFLVRLPIVGQVGSLNAAIAGSIAIYH
AWRQRHRPVTD

Specific function: Unknown

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Homo sapiens, GI40068479, Length=262, Percent_Identity=30.1526717557252, Blast_Score=100, Evalue=1e-21,
Organism=Escherichia coli, GI1790623, Length=245, Percent_Identity=35.1020408163265, Blast_Score=138, Evalue=4e-34,
Organism=Escherichia coli, GI1790083, Length=155, Percent_Identity=28.3870967741935, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI1788935, Length=146, Percent_Identity=28.0821917808219, Blast_Score=63, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17555908, Length=254, Percent_Identity=27.1653543307087, Blast_Score=99, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6324775, Length=286, Percent_Identity=24.4755244755245, Blast_Score=82, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004441
- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 27741; Mature: 27610

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATELLYGRNAVRESLRARRRVFHRLYVSQSVQESGIIAEIINLAQQAGVPVERIERQTL
CCCCHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHH
DRRLRDANHQGVMLEAEPYPYVELDTCLNAAAERREAALLLLLDHLQDPQNIGTLLRTAE
HHHHHCCCCCCEEEECCCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
AVGCHGVVIPGRRAAEITPAVVNASSGAVEHLRVAMVTNLNQTIEELQHAGIWVVGLEQD
HCCCCEEEECCCCCCCCCHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCC
EQAQDIDAVDLDLPLALVIGAEGTGIARLVRERCDFLVRLPIVGQVGSLNAAIAGSIAIY
CCCCCCCEECCCCCEEEEECCCCCHHHHHHHHHHHHEEECCCCCCCCCCCHHHHHHHHHH
HAWRQRHRPVTD
HHHHHHCCCCCC
>Mature Secondary Structure 
ATELLYGRNAVRESLRARRRVFHRLYVSQSVQESGIIAEIINLAQQAGVPVERIERQTL
CCCHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHH
DRRLRDANHQGVMLEAEPYPYVELDTCLNAAAERREAALLLLLDHLQDPQNIGTLLRTAE
HHHHHCCCCCCEEEECCCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
AVGCHGVVIPGRRAAEITPAVVNASSGAVEHLRVAMVTNLNQTIEELQHAGIWVVGLEQD
HCCCCEEEECCCCCCCCCHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCC
EQAQDIDAVDLDLPLALVIGAEGTGIARLVRERCDFLVRLPIVGQVGSLNAAIAGSIAIY
CCCCCCCEECCCCCEEEEECCCCCHHHHHHHHHHHHEEECCCCCCCCCCCHHHHHHHHHH
HAWRQRHRPVTD
HHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377; 7510287; 3145906 [H]