Click here to switch to the map view.

The map label for this gene is gdhA [H]

Identifier: 222525483

GI number: 222525483

Start: 2822703

End: 2823968

Strand: Reverse

Name: gdhA [H]

Synonym: Chy400_2231

Alternate gene names: 222525483

Gene position: 2823968-2822703 (Counterclockwise)

Preceding gene: 222525484

Following gene: 222525482

Centisome position: 53.6

GC content: 56.56

Gene sequence:

>1266_bases
ATGACGTCTCACCAGATTAATCCGTTCCACGTCGCGCAACGACAGTTCGATCAGGCCGCCGAGATGCTGAATCTGCCCGA
TGATATTCGCGCTATTCTGCGCGTGCCCCAGCGGGAGCTGACGGTCAACTTCCCGGTGCAGATGGACGATGGTACCACTC
AGGTCTTCACCGGTTATCGGGTACAACACAATCTGAGTCGTGGCCCGGTAAAGGGCGGTATCCGCTACCATCCCAGTGTT
GATATCGATGAAGTGCGAGCACTGGCAATGTGGATGACCTGGAAGTGTGCGCTGGTCAATATTCCCTACGGTGGTGCGAA
GGGTGGGGTGATCGTCGATCCCAAGCAACTGTCAATCGGCGAACTGGAACGGCTGACCCGGCGTTTTGCCACTGAAATCA
GCATTCTGCTCGGCCCAGAAAAAGATATTCCTGCGCCCGATGTTGGTACCAATGCCCAGGTGATGGCCTGGATTATGGAC
ACGATTTCGATGCATCGCGGCTACACCGTACCTGCTGTCATCACCGGCAAGCCGGTCAATGTTGGCGGCTCGTTAGGCCG
GGTAGAGGCAACCGGGCGCGGTGTGATGCTGATGGTTCGCGAAATGGCGCGTAAACTCGACTGGTCGCTCGAAGGATTGC
GCATCGTTGTGCAGGGGTTTGGCAACGTCGGTAGCACAGCGGCGTATCTGCTGCATCAACTCGGCTGTAAGATTATTGGG
GTTGCTGATGCCTCTGGCGGTTACTACTGTGCCCACGGGCTTGACATTCCAGCCATGCGGGCATACACCGACCGCCAGTC
GTTCCATTTGCTTGAGGGGTACCAGGCGCCAGGGGTAGAACGTATCAGCGGGAGCGAATTGCTCGAATTGGAGTGTGATG
TTCTGATCCCGGCAGCGCTCGAAAACCAGATTACCGGTAACAATGCCGAACGTATTCGCGCCAAACTCATTGTGGAAGGG
GCAAACGGGCCGACAACCCCCGACGCCGATGCCATTCTCGGTGAGCGGGGGATCATCATCGTACCCGACATCCTGGCTAA
CGCCGGTGGAGTGATTGTCTCCTATTTCGAGTGGGTGCAGGGCCTGCAAGAGTTCTTCTGGGACGAACAAGACATCAACG
AGAAACTGGAACGCATTATTGTCGGTGCATTTGATCAGGTGTACGCAATGGCTACCCAACGCCAGATTCCGCTCCGTCTC
GCTGCATATCTCCTGGCGGTACAGCGAGTGGCCGATGCTAACACGACGCGCGGAGTCTATCCATAA

Upstream 100 bases:

>100_bases
GTGAGCCGGTAGTAACCCTTATCGTCTTCAAGGGCGATAGAGTCATAAATAATATGAAGCACAGGTTACAACGAATTGCA
TCAATGAAGAGGAAAAGACA

Downstream 100 bases:

>100_bases
AGCAACGTGGGTGGTGCAATGAGGGTTCGATAGTGCCGGCATTCCCTCATTGCACCACAACGGGAGAGAATCATGCAGAT
CGTGCTCGTCGAAGATAATC

Product: Glu/Leu/Phe/Val dehydrogenase

Products: NA

Alternate protein names: GDH [H]

Number of amino acids: Translated: 421; Mature: 420

Protein sequence:

>421_residues
MTSHQINPFHVAQRQFDQAAEMLNLPDDIRAILRVPQRELTVNFPVQMDDGTTQVFTGYRVQHNLSRGPVKGGIRYHPSV
DIDEVRALAMWMTWKCALVNIPYGGAKGGVIVDPKQLSIGELERLTRRFATEISILLGPEKDIPAPDVGTNAQVMAWIMD
TISMHRGYTVPAVITGKPVNVGGSLGRVEATGRGVMLMVREMARKLDWSLEGLRIVVQGFGNVGSTAAYLLHQLGCKIIG
VADASGGYYCAHGLDIPAMRAYTDRQSFHLLEGYQAPGVERISGSELLELECDVLIPAALENQITGNNAERIRAKLIVEG
ANGPTTPDADAILGERGIIIVPDILANAGGVIVSYFEWVQGLQEFFWDEQDINEKLERIIVGAFDQVYAMATQRQIPLRL
AAYLLAVQRVADANTTRGVYP

Sequences:

>Translated_421_residues
MTSHQINPFHVAQRQFDQAAEMLNLPDDIRAILRVPQRELTVNFPVQMDDGTTQVFTGYRVQHNLSRGPVKGGIRYHPSV
DIDEVRALAMWMTWKCALVNIPYGGAKGGVIVDPKQLSIGELERLTRRFATEISILLGPEKDIPAPDVGTNAQVMAWIMD
TISMHRGYTVPAVITGKPVNVGGSLGRVEATGRGVMLMVREMARKLDWSLEGLRIVVQGFGNVGSTAAYLLHQLGCKIIG
VADASGGYYCAHGLDIPAMRAYTDRQSFHLLEGYQAPGVERISGSELLELECDVLIPAALENQITGNNAERIRAKLIVEG
ANGPTTPDADAILGERGIIIVPDILANAGGVIVSYFEWVQGLQEFFWDEQDINEKLERIIVGAFDQVYAMATQRQIPLRL
AAYLLAVQRVADANTTRGVYP
>Mature_420_residues
TSHQINPFHVAQRQFDQAAEMLNLPDDIRAILRVPQRELTVNFPVQMDDGTTQVFTGYRVQHNLSRGPVKGGIRYHPSVD
IDEVRALAMWMTWKCALVNIPYGGAKGGVIVDPKQLSIGELERLTRRFATEISILLGPEKDIPAPDVGTNAQVMAWIMDT
ISMHRGYTVPAVITGKPVNVGGSLGRVEATGRGVMLMVREMARKLDWSLEGLRIVVQGFGNVGSTAAYLLHQLGCKIIGV
ADASGGYYCAHGLDIPAMRAYTDRQSFHLLEGYQAPGVERISGSELLELECDVLIPAALENQITGNNAERIRAKLIVEGA
NGPTTPDADAILGERGIIIVPDILANAGGVIVSYFEWVQGLQEFFWDEQDINEKLERIIVGAFDQVYAMATQRQIPLRLA
AYLLAVQRVADANTTRGVYP

Specific function: Unknown

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=372, Percent_Identity=43.2795698924731, Blast_Score=297, Evalue=1e-80,
Organism=Homo sapiens, GI4885281, Length=372, Percent_Identity=43.2795698924731, Blast_Score=295, Evalue=8e-80,
Organism=Escherichia coli, GI1788059, Length=384, Percent_Identity=30.9895833333333, Blast_Score=176, Evalue=4e-45,
Organism=Caenorhabditis elegans, GI17544676, Length=392, Percent_Identity=39.030612244898, Blast_Score=271, Evalue=6e-73,
Organism=Saccharomyces cerevisiae, GI6324951, Length=387, Percent_Identity=30.2325581395349, Blast_Score=166, Evalue=7e-42,
Organism=Saccharomyces cerevisiae, GI6319256, Length=387, Percent_Identity=29.4573643410853, Blast_Score=161, Evalue=1e-40,
Organism=Drosophila melanogaster, GI24649283, Length=352, Percent_Identity=42.8977272727273, Blast_Score=280, Evalue=1e-75,
Organism=Drosophila melanogaster, GI45549226, Length=341, Percent_Identity=42.2287390029325, Blast_Score=276, Evalue=2e-74,
Organism=Drosophila melanogaster, GI45553475, Length=341, Percent_Identity=42.2287390029325, Blast_Score=276, Evalue=2e-74,
Organism=Drosophila melanogaster, GI24649460, Length=286, Percent_Identity=42.6573426573427, Blast_Score=228, Evalue=8e-60,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR014362
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.3 [H]

Molecular weight: Translated: 46176; Mature: 46045

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSHQINPFHVAQRQFDQAAEMLNLPDDIRAILRVPQRELTVNFPVQMDDGTTQVFTGYR
CCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCEEEECCEEECCCCEEEEECEE
VQHNLSRGPVKGGIRYHPSVDIDEVRALAMWMTWKCALVNIPYGGAKGGVIVDPKQLSIG
EEECCCCCCCCCCEEECCCCCHHHHHHHHHHHEEEEEEEECCCCCCCCCEEECCCCCCHH
ELERLTRRFATEISILLGPEKDIPAPDVGTNAQVMAWIMDTISMHRGYTVPAVITGKPVN
HHHHHHHHHHHHEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEECCCEE
VGGSLGRVEATGRGVMLMVREMARKLDWSLEGLRIVVQGFGNVGSTAAYLLHQLGCKIIG
CCCCCCEEEECCCCHHHHHHHHHHHHCCCHHHHEEEEECCCCCCHHHHHHHHHCCCEEEE
VADASGGYYCAHGLDIPAMRAYTDRQSFHLLEGYQAPGVERISGSELLELECDVLIPAAL
EEECCCCEEEECCCCCCHHHHHCCCCCCHHHCCCCCCCCCCCCCCCEEEEEECEEECHHH
ENQITGNNAERIRAKLIVEGANGPTTPDADAILGERGIIIVPDILANAGGVIVSYFEWVQ
CCCCCCCCHHEEEEEEEEECCCCCCCCCCHHHHCCCCEEEECHHHCCCCCHHHHHHHHHH
GLQEFFWDEQDINEKLERIIVGAFDQVYAMATQRQIPLRLAAYLLAVQRVADANTTRGVY
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCC
P
C
>Mature Secondary Structure 
TSHQINPFHVAQRQFDQAAEMLNLPDDIRAILRVPQRELTVNFPVQMDDGTTQVFTGYR
CCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCEEEECCEEECCCCEEEEECEE
VQHNLSRGPVKGGIRYHPSVDIDEVRALAMWMTWKCALVNIPYGGAKGGVIVDPKQLSIG
EEECCCCCCCCCCEEECCCCCHHHHHHHHHHHEEEEEEEECCCCCCCCCEEECCCCCCHH
ELERLTRRFATEISILLGPEKDIPAPDVGTNAQVMAWIMDTISMHRGYTVPAVITGKPVN
HHHHHHHHHHHHEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEECCCEE
VGGSLGRVEATGRGVMLMVREMARKLDWSLEGLRIVVQGFGNVGSTAAYLLHQLGCKIIG
CCCCCCEEEECCCCHHHHHHHHHHHHCCCHHHHEEEEECCCCCCHHHHHHHHHCCCEEEE
VADASGGYYCAHGLDIPAMRAYTDRQSFHLLEGYQAPGVERISGSELLELECDVLIPAAL
EEECCCCEEEECCCCCCHHHHHCCCCCCHHHCCCCCCCCCCCCCCCEEEEEECEEECHHH
ENQITGNNAERIRAKLIVEGANGPTTPDADAILGERGIIIVPDILANAGGVIVSYFEWVQ
CCCCCCCCHHEEEEEEEEECCCCCCCCCCHHHHCCCCEEEECHHHCCCCCHHHHHHHHHH
GLQEFFWDEQDINEKLERIIVGAFDQVYAMATQRQIPLRLAAYLLAVQRVADANTTRGVY
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCC
P
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9680336; 10360571; 9135121; 9654452; 10366510 [H]