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The map label for this gene is pnp

Identifier: 222525460

GI number: 222525460

Start: 2798238

End: 2800505

Strand: Direct

Name: pnp

Synonym: Chy400_2208

Alternate gene names: 222525460

Gene position: 2798238-2800505 (Clockwise)

Preceding gene: 222525459

Following gene: 222525461

Centisome position: 53.11

GC content: 56.48

Gene sequence:

>2268_bases
ATGACAGAACGGAATATCTATTCGGTAAGTGCGGAGATTGCCGGCCGAACGCTCACCCTGGAAGCCGGGCGTTTTGCCGA
ACAGGCTGATGGTGCTGTTGTAGCCCGGTATGGCGATACGATGCTGTTGGCCACCGTCGTTTGCGCCAAAGAAGCTCGTG
AAGGAACCGATTTCTTCCCGCTGACCGTTGATTACGAAGAGAAGATGTACGCGGTCGGCAAAATTCCGGGTAATTTCTTC
AAGCGCGAAGGTCGGCCCACCACCACGGCGATTCTCATCTCCCGCTTGACCGACCGCCCCCTGCGGCCACTTTTCCCCAA
AGGTTTTTACAACGAGGTTCAGGTCATTATCACGACGTTCTCGATTGATATGGAGAACGATCCGGGGCCGTTGGCAATAA
TTGCCGCTTCCGCAGCGCTGTGTATCAGCGATATTCCCTTCGCCGGCCCGGTTGGTGCGGTGCAGATGGGGCATCTGAAC
GGTCAGTTGGTGGTCAATCCCAAGATGAATGAGATTGCCGATAGCCGGCTCGATCTGGTGGTGGCCGGTACCAAAGATGC
AGTGCTGATGGTTGAAGCCGGCGCTTACGAGTTGACCGAAGACGAGATGCTTCAGGCCGTGATTGATGGCCATGCGGTCT
GCAAGCAGATCTGTGATCTACAAGAGCAACTGGTTCAGCTTTGTGGCAAACCGAAGCGTCCCTTCACGCCGCCGGTGGTT
GACACCTCGCTCGAAGAGGCCATCAGTGCGTGGATGGGTGACCGGCTGCGCAAAGCGGTACGCAGTCCAATCAAGCAAGA
ACGTGAAGCTCAAACGGAAGCGCTGAAGGCTGAAGTGATTGCCCACTTTACTGCTGATGAACCGGAAGAAGAGATCGCGA
ACCGCACCAAAGAGGTCACAAAGGCGTTTGAGAAGCTTCTGAAAGATGAAGTGCGTAACGCTATTCTCGATGAAGGTATC
CGGGTCGATGGCCGGGCGCTCGATGAAATTCGTCCGATCAGTATCGAAGTCGGGGTGATCCCTCGTGTGCATGGCTCGGC
GGTCTTTACCCGCGGTCAGACCCAGGTGTTGACGATTACCACCCTCGGTTCGCCCGGCGACGAGCAGAAAGTGGACGATC
TCGGTATCGAGACTTCCAAACGCTACATTCATCACTACAATTTCCCACCTTTTAGCACGGGCGAAGTGCGGCGGATTGGG
ACACCACGACGGCGTGATATTGGTCACGGTGCATTGGCCGAACGCTCGCTGTACGCCGTGTTGCCTGATGAGAAGGATTT
TCCCTACACGATCCGGCTGGTATCTGAGGTGCTCTCCTCGAATGGCTCGTCATCAATGGCGTCGGTCTGTGGTTCATCGC
TGAGCCTGATGGATGCCGGTGTACCGATCAAGGCGCCGGTTGCCGGTGTGGCCATGGGTCTGATTACCGGCGAAGATGGC
CGCTGGCGCGTGTTGACCGATATTCAGGGCCTGGAAGATGCCCTCGGCGATATGGACTTCAAAGTGGCCGGTACGGCAAA
AGGTGTTACCGGTTTGCAGATGGATATCAAGACAACCGGTATCACCTATGAGATTATGCGCGAGGCGTTTGCACAGGCCC
GTGCCGGTCGCCTGTTCATTCTTGATAAGATGAACGAGGTGATCAGTGCACCACGGCCAGAGCTGTCAATCTATGCACCG
CGCATTATGACGATCCAGATTCCGGTTGATAAGATTGGCGCCCTGATTGGCCCCGGCGGTAAGACGATCCGCAATATCTG
TGAGACGACCGGTGCGCAGATTGATATTGAAGACGATGGCCGTGTCTTTATCACAACCCCTGATGGTGCCGCAGCCAGGC
AGGCGATCAGCATGATCGAGGGGTTGACCCGCGAAGCCAAGGTTGGTGACATTTTCCTCGGTAAAGTGGTCAGCATTAAG
CCTTTTGGGGCGTTTGTGAATATCTTGCCCGGTAAAGACGGCATGGTTCACGTCTCGGAGCTTGATGAAAAGCGGGTCGA
GAACGTTGAGGATGTGGTCTCGCTCGGCGATGAAATCAATGTGATGGTGATCGATATTGATCGCACCACAGGGAAGATTA
GTCTTAGCCGACGGGCAGTGCTGACCGGCGAAACGCCCGAAGAGCGCAAAGCTGCTGGTGCCGCACCACGCCCGCGTCCA
CGCGAAGAACAGCGTGGTGGTCGCGATGAGCCGCGAAGCCTGCGTGACGAGCTGCGTGGACCGCGCCGTGAAGGTGACCG
GCCACGCCCGCGCCGCCGGGATGATTAG

Upstream 100 bases:

>100_bases
TACACCAGTGCCGAACTCCGCGCACCGGTCGCAACCGGCGACCGGATACCGTTGATTGGGTGCAAGGGCACCCTGGTCTG
GTAAGGAGTAATAGGAGTTT

Downstream 100 bases:

>100_bases
ATGGAGAGGAGGGTGTGATGCCACAAAGCTCCGTGTGAAATCGCGGTAATTGCTTTGTGGCTCACATTGCGGTTAGAGTG
GCTGTGAGCAGTTTGCAAAT

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase

Number of amino acids: Translated: 755; Mature: 754

Protein sequence:

>755_residues
MTERNIYSVSAEIAGRTLTLEAGRFAEQADGAVVARYGDTMLLATVVCAKEAREGTDFFPLTVDYEEKMYAVGKIPGNFF
KREGRPTTTAILISRLTDRPLRPLFPKGFYNEVQVIITTFSIDMENDPGPLAIIAASAALCISDIPFAGPVGAVQMGHLN
GQLVVNPKMNEIADSRLDLVVAGTKDAVLMVEAGAYELTEDEMLQAVIDGHAVCKQICDLQEQLVQLCGKPKRPFTPPVV
DTSLEEAISAWMGDRLRKAVRSPIKQEREAQTEALKAEVIAHFTADEPEEEIANRTKEVTKAFEKLLKDEVRNAILDEGI
RVDGRALDEIRPISIEVGVIPRVHGSAVFTRGQTQVLTITTLGSPGDEQKVDDLGIETSKRYIHHYNFPPFSTGEVRRIG
TPRRRDIGHGALAERSLYAVLPDEKDFPYTIRLVSEVLSSNGSSSMASVCGSSLSLMDAGVPIKAPVAGVAMGLITGEDG
RWRVLTDIQGLEDALGDMDFKVAGTAKGVTGLQMDIKTTGITYEIMREAFAQARAGRLFILDKMNEVISAPRPELSIYAP
RIMTIQIPVDKIGALIGPGGKTIRNICETTGAQIDIEDDGRVFITTPDGAAARQAISMIEGLTREAKVGDIFLGKVVSIK
PFGAFVNILPGKDGMVHVSELDEKRVENVEDVVSLGDEINVMVIDIDRTTGKISLSRRAVLTGETPEERKAAGAAPRPRP
REEQRGGRDEPRSLRDELRGPRREGDRPRPRRRDD

Sequences:

>Translated_755_residues
MTERNIYSVSAEIAGRTLTLEAGRFAEQADGAVVARYGDTMLLATVVCAKEAREGTDFFPLTVDYEEKMYAVGKIPGNFF
KREGRPTTTAILISRLTDRPLRPLFPKGFYNEVQVIITTFSIDMENDPGPLAIIAASAALCISDIPFAGPVGAVQMGHLN
GQLVVNPKMNEIADSRLDLVVAGTKDAVLMVEAGAYELTEDEMLQAVIDGHAVCKQICDLQEQLVQLCGKPKRPFTPPVV
DTSLEEAISAWMGDRLRKAVRSPIKQEREAQTEALKAEVIAHFTADEPEEEIANRTKEVTKAFEKLLKDEVRNAILDEGI
RVDGRALDEIRPISIEVGVIPRVHGSAVFTRGQTQVLTITTLGSPGDEQKVDDLGIETSKRYIHHYNFPPFSTGEVRRIG
TPRRRDIGHGALAERSLYAVLPDEKDFPYTIRLVSEVLSSNGSSSMASVCGSSLSLMDAGVPIKAPVAGVAMGLITGEDG
RWRVLTDIQGLEDALGDMDFKVAGTAKGVTGLQMDIKTTGITYEIMREAFAQARAGRLFILDKMNEVISAPRPELSIYAP
RIMTIQIPVDKIGALIGPGGKTIRNICETTGAQIDIEDDGRVFITTPDGAAARQAISMIEGLTREAKVGDIFLGKVVSIK
PFGAFVNILPGKDGMVHVSELDEKRVENVEDVVSLGDEINVMVIDIDRTTGKISLSRRAVLTGETPEERKAAGAAPRPRP
REEQRGGRDEPRSLRDELRGPRREGDRPRPRRRDD
>Mature_754_residues
TERNIYSVSAEIAGRTLTLEAGRFAEQADGAVVARYGDTMLLATVVCAKEAREGTDFFPLTVDYEEKMYAVGKIPGNFFK
REGRPTTTAILISRLTDRPLRPLFPKGFYNEVQVIITTFSIDMENDPGPLAIIAASAALCISDIPFAGPVGAVQMGHLNG
QLVVNPKMNEIADSRLDLVVAGTKDAVLMVEAGAYELTEDEMLQAVIDGHAVCKQICDLQEQLVQLCGKPKRPFTPPVVD
TSLEEAISAWMGDRLRKAVRSPIKQEREAQTEALKAEVIAHFTADEPEEEIANRTKEVTKAFEKLLKDEVRNAILDEGIR
VDGRALDEIRPISIEVGVIPRVHGSAVFTRGQTQVLTITTLGSPGDEQKVDDLGIETSKRYIHHYNFPPFSTGEVRRIGT
PRRRDIGHGALAERSLYAVLPDEKDFPYTIRLVSEVLSSNGSSSMASVCGSSLSLMDAGVPIKAPVAGVAMGLITGEDGR
WRVLTDIQGLEDALGDMDFKVAGTAKGVTGLQMDIKTTGITYEIMREAFAQARAGRLFILDKMNEVISAPRPELSIYAPR
IMTIQIPVDKIGALIGPGGKTIRNICETTGAQIDIEDDGRVFITTPDGAAARQAISMIEGLTREAKVGDIFLGKVVSIKP
FGAFVNILPGKDGMVHVSELDEKRVENVEDVVSLGDEINVMVIDIDRTTGKISLSRRAVLTGETPEERKAAGAAPRPRPR
EEQRGGRDEPRSLRDELRGPRREGDRPRPRRRDD

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain

Homologues:

Organism=Homo sapiens, GI188528628, Length=719, Percent_Identity=37.9694019471488, Blast_Score=449, Evalue=1e-126,
Organism=Homo sapiens, GI9506689, Length=230, Percent_Identity=26.9565217391304, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI145693187, Length=682, Percent_Identity=51.6129032258064, Blast_Score=664, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115534063, Length=720, Percent_Identity=35.9722222222222, Blast_Score=381, Evalue=1e-106,
Organism=Drosophila melanogaster, GI281362905, Length=712, Percent_Identity=38.7640449438202, Blast_Score=455, Evalue=1e-128,
Organism=Drosophila melanogaster, GI24651641, Length=712, Percent_Identity=38.7640449438202, Blast_Score=455, Evalue=1e-128,
Organism=Drosophila melanogaster, GI24651643, Length=712, Percent_Identity=38.7640449438202, Blast_Score=455, Evalue=1e-128,
Organism=Drosophila melanogaster, GI161079377, Length=657, Percent_Identity=38.5083713850837, Blast_Score=413, Evalue=1e-115,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): PNP_CHLAA (A9WEJ7)

Other databases:

- EMBL:   CP000909
- RefSeq:   YP_001635648.1
- ProteinModelPortal:   A9WEJ7
- SMR:   A9WEJ7
- GeneID:   5826502
- GenomeReviews:   CP000909_GR
- KEGG:   cau:Caur_2047
- HOGENOM:   HBG382411
- OMA:   YGETVVL
- ProtClustDB:   PRK11824
- GO:   GO:0005739
- HAMAP:   MF_01595
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:1.10.10.400
- PANTHER:   PTHR11252
- PIRSF:   PIRSF005499
- SMART:   SM00322
- SMART:   SM00316
- TIGRFAMs:   TIGR03591

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1; SSF46915 3_ExoRNase; SSF55666 3_ExoRNase; SSF50249 Nucleic_acid_OB; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.8

Molecular weight: Translated: 82433; Mature: 82302

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS50084 KH_TYPE_1; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTERNIYSVSAEIAGRTLTLEAGRFAEQADGAVVARYGDTMLLATVVCAKEAREGTDFFP
CCCCCEEEEEHHHCCCEEEEECCCCHHHCCCEEEEECCCHHHHHHHHHHHHHCCCCCEEE
LTVDYEEKMYAVGKIPGNFFKREGRPTTTAILISRLTDRPLRPLFPKGFYNEVQVIITTF
EEECCCCCEEEEECCCHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEE
SIDMENDPGPLAIIAASAALCISDIPFAGPVGAVQMGHLNGQLVVNPKMNEIADSRLDLV
EEEECCCCCCEEEEEHHHHHHHHCCCCCCCCCCEEEECCCCEEEECCCHHHHCCCCEEEE
VAGTKDAVLMVEAGAYELTEDEMLQAVIDGHAVCKQICDLQEQLVQLCGKPKRPFTPPVV
EECCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
DTSLEEAISAWMGDRLRKAVRSPIKQEREAQTEALKAEVIAHFTADEPEEEIANRTKEVT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHH
KAFEKLLKDEVRNAILDEGIRVDGRALDEIRPISIEVGVIPRVHGSAVFTRGQTQVLTIT
HHHHHHHHHHHHHHHHHCCCCCCCCHHCCCCCEEEEEEECCCCCCCEEEECCCEEEEEEE
TLGSPGDEQKVDDLGIETSKRYIHHYNFPPFSTGEVRRIGTPRRRDIGHGALAERSLYAV
ECCCCCCCCCHHHCCCCCCCHHEEECCCCCCCCCCEEECCCCCCCCCCCCCCCCCCEEEE
LPDEKDFPYTIRLVSEVLSSNGSSSMASVCGSSLSLMDAGVPIKAPVAGVAMGLITGEDG
CCCCCCCCCHHHHHHHHHHCCCCCHHHHHHCCCHHHHCCCCCCCCCHHHHEEEEEECCCC
RWRVLTDIQGLEDALGDMDFKVAGTAKGVTGLQMDIKTTGITYEIMREAFAQARAGRLFI
CEEEEECHHHHHHHHCCCCEEEECCCCCCCEEEEEEEECCCHHHHHHHHHHHHCCCEEEE
LDKMNEVISAPRPELSIYAPRIMTIQIPVDKIGALIGPGGKTIRNICETTGAQIDIEDDG
EEHHHHHHCCCCCCEEEECCEEEEEEECHHHHHHEECCCHHHHHHHHHHCCCEEEECCCC
RVFITTPDGAAARQAISMIEGLTREAKVGDIFLGKVVSIKPFGAFVNILPGKDGMVHVSE
EEEEECCCCHHHHHHHHHHHHHCCCCCCCCEEECCEEEEECCCEEEEEECCCCCEEEHHH
LDEKRVENVEDVVSLGDEINVMVIDIDRTTGKISLSRRAVLTGETPEERKAAGAAPRPRP
HHHHHHHHHHHHHHCCCCCEEEEEEECCCCCEEEECCEEEEECCCCHHHHHCCCCCCCCC
REEQRGGRDEPRSLRDELRGPRREGDRPRPRRRDD
CHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TERNIYSVSAEIAGRTLTLEAGRFAEQADGAVVARYGDTMLLATVVCAKEAREGTDFFP
CCCCEEEEEHHHCCCEEEEECCCCHHHCCCEEEEECCCHHHHHHHHHHHHHCCCCCEEE
LTVDYEEKMYAVGKIPGNFFKREGRPTTTAILISRLTDRPLRPLFPKGFYNEVQVIITTF
EEECCCCCEEEEECCCHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEE
SIDMENDPGPLAIIAASAALCISDIPFAGPVGAVQMGHLNGQLVVNPKMNEIADSRLDLV
EEEECCCCCCEEEEEHHHHHHHHCCCCCCCCCCEEEECCCCEEEECCCHHHHCCCCEEEE
VAGTKDAVLMVEAGAYELTEDEMLQAVIDGHAVCKQICDLQEQLVQLCGKPKRPFTPPVV
EECCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
DTSLEEAISAWMGDRLRKAVRSPIKQEREAQTEALKAEVIAHFTADEPEEEIANRTKEVT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHH
KAFEKLLKDEVRNAILDEGIRVDGRALDEIRPISIEVGVIPRVHGSAVFTRGQTQVLTIT
HHHHHHHHHHHHHHHHHCCCCCCCCHHCCCCCEEEEEEECCCCCCCEEEECCCEEEEEEE
TLGSPGDEQKVDDLGIETSKRYIHHYNFPPFSTGEVRRIGTPRRRDIGHGALAERSLYAV
ECCCCCCCCCHHHCCCCCCCHHEEECCCCCCCCCCEEECCCCCCCCCCCCCCCCCCEEEE
LPDEKDFPYTIRLVSEVLSSNGSSSMASVCGSSLSLMDAGVPIKAPVAGVAMGLITGEDG
CCCCCCCCCHHHHHHHHHHCCCCCHHHHHHCCCHHHHCCCCCCCCCHHHHEEEEEECCCC
RWRVLTDIQGLEDALGDMDFKVAGTAKGVTGLQMDIKTTGITYEIMREAFAQARAGRLFI
CEEEEECHHHHHHHHCCCCEEEECCCCCCCEEEEEEEECCCHHHHHHHHHHHHCCCEEEE
LDKMNEVISAPRPELSIYAPRIMTIQIPVDKIGALIGPGGKTIRNICETTGAQIDIEDDG
EEHHHHHHCCCCCCEEEECCEEEEEEECHHHHHHEECCCHHHHHHHHHHCCCEEEECCCC
RVFITTPDGAAARQAISMIEGLTREAKVGDIFLGKVVSIKPFGAFVNILPGKDGMVHVSE
EEEEECCCCHHHHHHHHHHHHHCCCCCCCCEEECCEEEEECCCEEEEEECCCCCEEEHHH
LDEKRVENVEDVVSLGDEINVMVIDIDRTTGKISLSRRAVLTGETPEERKAAGAAPRPRP
HHHHHHHHHHHHHHCCCCCEEEEEEECCCCCEEEECCEEEEECCCCHHHHHCCCCCCCCC
REEQRGGRDEPRSLRDELRGPRREGDRPRPRRRDD
CHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA