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The map label for this gene is nuoB1

Identifier: 222525396

GI number: 222525396

Start: 2719695

End: 2720489

Strand: Reverse

Name: nuoB1

Synonym: Chy400_2141

Alternate gene names: 222525396

Gene position: 2720489-2719695 (Counterclockwise)

Preceding gene: 222525397

Following gene: 222525395

Centisome position: 51.63

GC content: 58.99

Gene sequence:

>795_bases
ATGGCTGAGATTGATCCAACCCAGCTCGAACTCGAACGCCAGGGTATCTTGTTGACGACGGTGAACCGGTTTTATAACTG
GGGTCGTCGTTCGTCGATCTGGCCTATGGCGTTTGGGCTGGCGTGTTGTGCAATTGAGATGATGGCGTTCGGGTTGAGTC
GCTATGACGTTGCCCGGTTTGGCGCCGAGCTGTTTCGGGCGTCGCCGCGGCAGGCCGATCTGATGATCGTCGCCGGCACG
GTGACGAAGAAGATGGCCCCCCAGGTGGTGCGGCTCTATAATCAGATGGCCGAGCCGCGGTATGTGATCTCGATGGGGGC
CTGCGCAACATCCGGTGGCCCCTTCCGTGATGGGTACAATGTTCTGCGTGGTATCGACCTGTTGATTCCAGTTGATGTGT
ATGTACCCGGCTGCCCCCCACGCCCTGAAGCACTCCTGCACGCTCTGATGACGTTGCAAGAGCAGATTGACCGGCAGAAG
CTTGGGCGAGTGCGCTGGTACGGGAGTGGCGATAAGCCGCAGACGGGCGACTTTCCGGTACCAACTTTTGGGGCGAAGGG
ATTGGAAATCGACGGCAAGCTGGTCGATCCGGTTGGTGGGTTGCCGTTGCTCAGCCCATATACATCGCCGAGCCATGGCG
AGCACCGTTCGGGGCAGATCGAGCATCCTGAAGTGGTACGCCAGTTCCCAATCATGGATCCCGAAGTAGAGCTTGAGAAT
GCGCTCAAGGCGCGTGGGATCGCTCCTGAGATCGCAGCCGACGACTTGAAACGGAGTGTGGTGAACGATGCCTGA

Upstream 100 bases:

>100_bases
TAGGTCTTTACGGCCTGATTGCAGCGACCATCTTCCTCCTGATGCTGTTTGCCGGTTTGCTCTACGAGTGGCGTAAAGGC
GCCCTAGAGTGGGTGTAACC

Downstream 100 bases:

>100_bases
ACTGACACCAACCGATCTGCGGACACGACTGGCCACGGCGCTGCCGGATGCCCTGATCGAGGTGGAACCCCTGGCGATCA
CGCCACCACCTGCTCACGGC

Product: NADH dehydrogenase subunit B

Products: NA

Alternate protein names: NADH dehydrogenase I subunit B 1; NDH-1 subunit B 1

Number of amino acids: Translated: 264; Mature: 263

Protein sequence:

>264_residues
MAEIDPTQLELERQGILLTTVNRFYNWGRRSSIWPMAFGLACCAIEMMAFGLSRYDVARFGAELFRASPRQADLMIVAGT
VTKKMAPQVVRLYNQMAEPRYVISMGACATSGGPFRDGYNVLRGIDLLIPVDVYVPGCPPRPEALLHALMTLQEQIDRQK
LGRVRWYGSGDKPQTGDFPVPTFGAKGLEIDGKLVDPVGGLPLLSPYTSPSHGEHRSGQIEHPEVVRQFPIMDPEVELEN
ALKARGIAPEIAADDLKRSVVNDA

Sequences:

>Translated_264_residues
MAEIDPTQLELERQGILLTTVNRFYNWGRRSSIWPMAFGLACCAIEMMAFGLSRYDVARFGAELFRASPRQADLMIVAGT
VTKKMAPQVVRLYNQMAEPRYVISMGACATSGGPFRDGYNVLRGIDLLIPVDVYVPGCPPRPEALLHALMTLQEQIDRQK
LGRVRWYGSGDKPQTGDFPVPTFGAKGLEIDGKLVDPVGGLPLLSPYTSPSHGEHRSGQIEHPEVVRQFPIMDPEVELEN
ALKARGIAPEIAADDLKRSVVNDA
>Mature_263_residues
AEIDPTQLELERQGILLTTVNRFYNWGRRSSIWPMAFGLACCAIEMMAFGLSRYDVARFGAELFRASPRQADLMIVAGTV
TKKMAPQVVRLYNQMAEPRYVISMGACATSGGPFRDGYNVLRGIDLLIPVDVYVPGCPPRPEALLHALMTLQEQIDRQKL
GRVRWYGSGDKPQTGDFPVPTFGAKGLEIDGKLVDPVGGLPLLSPYTSPSHGEHRSGQIEHPEVVRQFPIMDPEVELENA
LKARGIAPEIAADDLKRSVVNDA

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Cytoplasmic side

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family

Homologues:

Organism=Homo sapiens, GI187281616, Length=156, Percent_Identity=53.8461538461538, Blast_Score=187, Evalue=6e-48,
Organism=Escherichia coli, GI1788624, Length=156, Percent_Identity=57.6923076923077, Blast_Score=195, Evalue=2e-51,
Organism=Escherichia coli, GI1789074, Length=122, Percent_Identity=41.8032786885246, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1788834, Length=122, Percent_Identity=39.344262295082, Blast_Score=95, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17509685, Length=150, Percent_Identity=56, Blast_Score=180, Evalue=8e-46,
Organism=Drosophila melanogaster, GI18859983, Length=150, Percent_Identity=55.3333333333333, Blast_Score=185, Evalue=3e-47,
Organism=Drosophila melanogaster, GI24642371, Length=150, Percent_Identity=55.3333333333333, Blast_Score=185, Evalue=3e-47,
Organism=Drosophila melanogaster, GI24651058, Length=142, Percent_Identity=55.6338028169014, Blast_Score=179, Evalue=2e-45,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NUOB1_CHLAA (A9WED9)

Other databases:

- EMBL:   CP000909
- RefSeq:   YP_001635590.1
- ProteinModelPortal:   A9WED9
- SMR:   A9WED9
- GeneID:   5826437
- GenomeReviews:   CP000909_GR
- KEGG:   cau:Caur_1986
- HOGENOM:   HBG553221
- OMA:   NQMPEPR
- ProtClustDB:   PRK14819
- GO:   GO:0006810
- HAMAP:   MF_01356
- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406
- PANTHER:   PTHR11995:SF2
- PANTHER:   PTHR11995
- TIGRFAMs:   TIGR01957

Pfam domain/function: PF01058 Oxidored_q6

EC number: =1.6.99.5

Molecular weight: Translated: 29108; Mature: 28977

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEIDPTQLELERQGILLTTVNRFYNWGRRSSIWPMAFGLACCAIEMMAFGLSRYDVARF
CCCCCCHHHHHHHCCEEEEEHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHH
GAELFRASPRQADLMIVAGTVTKKMAPQVVRLYNQMAEPRYVISMGACATSGGPFRDGYN
HHHHHHCCCCCCCEEEEECHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHH
VLRGIDLLIPVDVYVPGCPPRPEALLHALMTLQEQIDRQKLGRVRWYGSGDKPQTGDFPV
HHHHHHEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCC
PTFGAKGLEIDGKLVDPVGGLPLLSPYTSPSHGEHRSGQIEHPEVVRQFPIMDPEVELEN
CCCCCCCEEECCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCHHHHH
ALKARGIAPEIAADDLKRSVVNDA
HHHHCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AEIDPTQLELERQGILLTTVNRFYNWGRRSSIWPMAFGLACCAIEMMAFGLSRYDVARF
CCCCCHHHHHHHCCEEEEEHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHH
GAELFRASPRQADLMIVAGTVTKKMAPQVVRLYNQMAEPRYVISMGACATSGGPFRDGYN
HHHHHHCCCCCCCEEEEECHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHH
VLRGIDLLIPVDVYVPGCPPRPEALLHALMTLQEQIDRQKLGRVRWYGSGDKPQTGDFPV
HHHHHHEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCC
PTFGAKGLEIDGKLVDPVGGLPLLSPYTSPSHGEHRSGQIEHPEVVRQFPIMDPEVELEN
CCCCCCCEEECCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCHHHHH
ALKARGIAPEIAADDLKRSVVNDA
HHHHCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA