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The map label for this gene is ybjF [C]

Identifier: 222525358

GI number: 222525358

Start: 2674140

End: 2675339

Strand: Direct

Name: ybjF [C]

Synonym: Chy400_2103

Alternate gene names: 222525358

Gene position: 2674140-2675339 (Clockwise)

Preceding gene: 222525357

Following gene: 222525360

Centisome position: 50.75

GC content: 55.83

Gene sequence:

>1200_bases
ATGCCACCTTTTACCACTAAAACCCTCCGTAAAGCAATCGTTGATCGCGTTCTGGCCGGTCAACCTGTGACGCCGCTGTG
CCCACATGCTCGTGAATGTGGTGGCTGTGCCTTTCAGGATCGCACTTATGCGGATCAGGTCGCGGCAAAAACGACGATCC
TGCGCATGCTGTACGCAGAAGGTGGGATTGACGCCTCGGCTTTAGAAGTCGTTGCCTCACCTGACCCGTTTGCCTACCGT
ACCCGGATGGATTACGTTGCCACCAAGGGGCGCTTCGGGCTGCGTGCTCGCGGTAAATTCAACTACATCATCGAATTGAC
GACGTGTCATCTAATCCCACCAACTGCCTTCGCTGCTGCCCAGGCAGTCTGGCGGCATGCGCTTGACCTGGGACTACCCG
ATTACGATGTGCGCACCCATACCGGTTTTTTGCGCTACGTTGTGGTACGGCGTAGTCCTGATGATGAGTTGTTGCTCGCT
GCCGTCACGGCTGGAGGCGGTGAGGAGGCTGTAATGGCAGATCTGGCTGCTACCGCCCTGGCTCAACCGGGAGTGGTCGG
CTTTCACTGGCTGGTCAACGACTCGCTCACCGATGTCTCGTTTGGCGAAGTATGCCGGTACTGGGGAGCTGATCGGTTGG
CAATGCGGGTTGGGCACTTTACCTGCTTTATCGGGCCAAATACCTTCTTTCAGAACAATATTCATCTGACCGAACGGCTG
ATCAATGACGCAGTTGCCGCAGCCGCACCTGATGCTACGATGCGGGTCGCCGATCTCTACGGTGGCGTTGGTGTTATTGG
TCTACATCTGGCACAATCAGCCGCGCGAGTCGATTGTGTCGAGTCAGTAGCTGAGAGTGTGCAGCTTGCCCACCAGAATG
CGCTGATGAATAAGGTGTCGAATATACATCCGATCTGTGCGGATACGCTGACCTACTTGCGGGAACAACCAACCGGCAGC
CTGCCAGTTGTGGTGGTTGATCCTCCACGTACCGGGTTAGGACTGCCGGTTTGCCAGGAATTAATCAGAATCGCCCCGCA
GCGGATTGTCTATGTCTCGTGTAATCCATTGACGCAGGTCAGCGATCTCCAACACTTGCAGTCGGCGTACCGGATTGTCG
GTTTACGCGGCTACGATATGTTTCCGCACACACCACACGTTGAAGTCATGGCAGTGCTGGAACGTCGCGTAAACGACTAG

Upstream 100 bases:

>100_bases
CGTCAGCGTGAGGGTATTGTTCTGAGGGCGATGTTCGTGAATGTAAACGTTGTGCTACTCGATCCCTGTTTTGAAACTGA
ATGCGAAGTACGCTTGATAT

Downstream 100 bases:

>100_bases
CCGCAACCGTCGGCATCAGAAAATCGCTACGGCGTTACCGTTAATGTTCCCTTCATACCGGCCAGATAGTGCCCGGGGAA
GGTACAAATGTAAAGGTATG

Product: (Uracil-5)-methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 399; Mature: 398

Protein sequence:

>399_residues
MPPFTTKTLRKAIVDRVLAGQPVTPLCPHARECGGCAFQDRTYADQVAAKTTILRMLYAEGGIDASALEVVASPDPFAYR
TRMDYVATKGRFGLRARGKFNYIIELTTCHLIPPTAFAAAQAVWRHALDLGLPDYDVRTHTGFLRYVVVRRSPDDELLLA
AVTAGGGEEAVMADLAATALAQPGVVGFHWLVNDSLTDVSFGEVCRYWGADRLAMRVGHFTCFIGPNTFFQNNIHLTERL
INDAVAAAAPDATMRVADLYGGVGVIGLHLAQSAARVDCVESVAESVQLAHQNALMNKVSNIHPICADTLTYLREQPTGS
LPVVVVDPPRTGLGLPVCQELIRIAPQRIVYVSCNPLTQVSDLQHLQSAYRIVGLRGYDMFPHTPHVEVMAVLERRVND

Sequences:

>Translated_399_residues
MPPFTTKTLRKAIVDRVLAGQPVTPLCPHARECGGCAFQDRTYADQVAAKTTILRMLYAEGGIDASALEVVASPDPFAYR
TRMDYVATKGRFGLRARGKFNYIIELTTCHLIPPTAFAAAQAVWRHALDLGLPDYDVRTHTGFLRYVVVRRSPDDELLLA
AVTAGGGEEAVMADLAATALAQPGVVGFHWLVNDSLTDVSFGEVCRYWGADRLAMRVGHFTCFIGPNTFFQNNIHLTERL
INDAVAAAAPDATMRVADLYGGVGVIGLHLAQSAARVDCVESVAESVQLAHQNALMNKVSNIHPICADTLTYLREQPTGS
LPVVVVDPPRTGLGLPVCQELIRIAPQRIVYVSCNPLTQVSDLQHLQSAYRIVGLRGYDMFPHTPHVEVMAVLERRVND
>Mature_398_residues
PPFTTKTLRKAIVDRVLAGQPVTPLCPHARECGGCAFQDRTYADQVAAKTTILRMLYAEGGIDASALEVVASPDPFAYRT
RMDYVATKGRFGLRARGKFNYIIELTTCHLIPPTAFAAAQAVWRHALDLGLPDYDVRTHTGFLRYVVVRRSPDDELLLAA
VTAGGGEEAVMADLAATALAQPGVVGFHWLVNDSLTDVSFGEVCRYWGADRLAMRVGHFTCFIGPNTFFQNNIHLTERLI
NDAVAAAAPDATMRVADLYGGVGVIGLHLAQSAARVDCVESVAESVQLAHQNALMNKVSNIHPICADTLTYLREQPTGSL
PVVVVDPPRTGLGLPVCQELIRIAPQRIVYVSCNPLTQVSDLQHLQSAYRIVGLRGYDMFPHTPHVEVMAVLERRVND

Specific function: Could Be A 23s rRNA (Uracil-5-)-Methyltransferase. [C]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI34222389, Length=361, Percent_Identity=27.7008310249307, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI51173878, Length=361, Percent_Identity=27.7008310249307, Blast_Score=84, Evalue=2e-16,
Organism=Escherichia coli, GI1787083, Length=380, Percent_Identity=26.0526315789474, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1789148, Length=414, Percent_Identity=29.4685990338164, Blast_Score=105, Evalue=6e-24,
Organism=Escherichia coli, GI1790403, Length=182, Percent_Identity=29.6703296703297, Blast_Score=65, Evalue=8e-12,
Organism=Drosophila melanogaster, GI24666579, Length=374, Percent_Identity=24.8663101604278, Blast_Score=73, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016027
- InterPro:   IPR001566
- InterPro:   IPR002792
- InterPro:   IPR010280 [H]

Pfam domain/function: PF01938 TRAM; PF05958 tRNA_U5-meth_tr [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 43518; Mature: 43386

Theoretical pI: Translated: 6.83; Mature: 6.83

Prosite motif: PS01230 TRMA_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPFTTKTLRKAIVDRVLAGQPVTPLCPHARECGGCAFQDRTYADQVAAKTTILRMLYAE
CCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHC
GGIDASALEVVASPDPFAYRTRMDYVATKGRFGLRARGKFNYIIELTTCHLIPPTAFAAA
CCCCHHHHEEEECCCCCHHHHHHHHHHCCCCCCEEECCCEEEEEEEEEEECCCCHHHHHH
QAVWRHALDLGLPDYDVRTHTGFLRYVVVRRSPDDELLLAAVTAGGGEEAVMADLAATAL
HHHHHHHHHCCCCCCCCHHCCCEEEEEEEECCCCCCEEEEEEECCCCCHHHHHHHHHHHH
AQPGVVGFHWLVNDSLTDVSFGEVCRYWGADRLAMRVGHFTCFIGPNTFFQNNIHLTERL
CCCCCEEEEEEECCCCCCCCHHHHHHHHCCHHHHHEECCEEEEECCCHHCCCCHHHHHHH
INDAVAAAAPDATMRVADLYGGVGVIGLHLAQSAARVDCVESVAESVQLAHQNALMNKVS
HHHHHHHCCCCCCEEHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NIHPICADTLTYLREQPTGSLPVVVVDPPRTGLGLPVCQELIRIAPQRIVYVSCNPLTQV
CCCCHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCHHHHHHHHHCCCEEEEEECCCCHHH
SDLQHLQSAYRIVGLRGYDMFPHTPHVEVMAVLERRVND
HHHHHHHHHHHEEEECCCCCCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure 
PPFTTKTLRKAIVDRVLAGQPVTPLCPHARECGGCAFQDRTYADQVAAKTTILRMLYAE
CCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHC
GGIDASALEVVASPDPFAYRTRMDYVATKGRFGLRARGKFNYIIELTTCHLIPPTAFAAA
CCCCHHHHEEEECCCCCHHHHHHHHHHCCCCCCEEECCCEEEEEEEEEEECCCCHHHHHH
QAVWRHALDLGLPDYDVRTHTGFLRYVVVRRSPDDELLLAAVTAGGGEEAVMADLAATAL
HHHHHHHHHCCCCCCCCHHCCCEEEEEEEECCCCCCEEEEEEECCCCCHHHHHHHHHHHH
AQPGVVGFHWLVNDSLTDVSFGEVCRYWGADRLAMRVGHFTCFIGPNTFFQNNIHLTERL
CCCCCEEEEEEECCCCCCCCHHHHHHHHCCHHHHHEECCEEEEECCCHHCCCCHHHHHHH
INDAVAAAAPDATMRVADLYGGVGVIGLHLAQSAARVDCVESVAESVQLAHQNALMNKVS
HHHHHHHCCCCCCEEHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NIHPICADTLTYLREQPTGSLPVVVVDPPRTGLGLPVCQELIRIAPQRIVYVSCNPLTQV
CCCCHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCHHHHHHHHHCCCEEEEEECCCCHHH
SDLQHLQSAYRIVGLRGYDMFPHTPHVEVMAVLERRVND
HHHHHHHHHHHEEEECCCCCCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]