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The map label for this gene is rmlA2 [C]

Identifier: 222525351

GI number: 222525351

Start: 2666663

End: 2667439

Strand: Reverse

Name: rmlA2 [C]

Synonym: Chy400_2096

Alternate gene names: 222525351

Gene position: 2667439-2666663 (Counterclockwise)

Preceding gene: 222525352

Following gene: 222525348

Centisome position: 50.63

GC content: 58.17

Gene sequence:

>777_bases
ATGAACACACCTGTGATCGGCTTGATTCCGGCTGGTGGGATGGCAACCCGTCTGGGGCCGTTACCGTGCAGCAAAGAACT
CTTGCCGGTTGGTTCCTTCCAAACCCCAGACGGCCTTCGTCCACGACCGGTCATTACCTATCTCCTCGCGCAGTGGCAAC
GGGCTGGTATTGGCCAGGCGCTGATTGTGGTCAGACCTGGCAAATGGGACATTCCTGCCTATCTGGGTGATGGGAGTGCC
TTTGGGCCGCGCCTCGCCTATCTGACCGTTCACGAACCGCACGGTGCAGCCTACACCCTGGCTACCGCTCTCCCTTTCAT
TCAAGATCGTACCGTTGTCCTCGGTTTACCTGACGTCATTCTGGCACCAGACGATGTCTATGCGACCCTCCTGAGTCACC
ACAGCACAGCTCAACCGGACCTCACCCTCGGTCTCTTTCCATGCACACACCCTCAATCAGCAGACATGGTTGCACTGGCG
GCTGACAGTTGTCGGGTTACAGCCATCGAGATCAAGCCTGCGCAAACCGATTTACAGTGGACGTGGATGGCGGCTATCTG
GGAGCCGACAGTAACCACCCTCTTAGGTGAGGTCGTAGCTGCTGACCGCACTGCGCGTACCCAGAGACAGACGTGCCCGG
AATTACACATTGGTGCCGTCTTCCAGGCCGCTATTGCCCGTGGCTTGCGTGTTGAAGGCGTTACCTTTGCCGACGGTAGA
GCACTCGATATTGGAACACCCGCGGGTTGGGAAGCGGCAGCGCACTTTACAGGTTGA

Upstream 100 bases:

>100_bases
GCCGCTTCGGACGTCAGACACCGGAATATCTGCCCTATGGTGCCTATTTGTGTCTGGGAGTGCTGATCTACGTCGCACTC
GGTAGTTACCGGTTGATGTG

Downstream 100 bases:

>100_bases
GATTGATAAGCTGCCGACAAGAACGCCGGATCATACTAACGCTTGTGCAACGATGCACATTGTTCAGGTGTTCCCCCCCG
TGCTAAGGCGCCACCAGCGG

Product: nucleotidyl transferase

Products: dTDPglucose; Diphosphate [C]

Alternate protein names: Glucose-1-Phosphate Thymidylyltransferase; UTP-Glucose-1-Phosphate Thymidylyltransferase; Nucleotidyltransferase; Glucose-1-Phosphate Thymidyltransferase

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MNTPVIGLIPAGGMATRLGPLPCSKELLPVGSFQTPDGLRPRPVITYLLAQWQRAGIGQALIVVRPGKWDIPAYLGDGSA
FGPRLAYLTVHEPHGAAYTLATALPFIQDRTVVLGLPDVILAPDDVYATLLSHHSTAQPDLTLGLFPCTHPQSADMVALA
ADSCRVTAIEIKPAQTDLQWTWMAAIWEPTVTTLLGEVVAADRTARTQRQTCPELHIGAVFQAAIARGLRVEGVTFADGR
ALDIGTPAGWEAAAHFTG

Sequences:

>Translated_258_residues
MNTPVIGLIPAGGMATRLGPLPCSKELLPVGSFQTPDGLRPRPVITYLLAQWQRAGIGQALIVVRPGKWDIPAYLGDGSA
FGPRLAYLTVHEPHGAAYTLATALPFIQDRTVVLGLPDVILAPDDVYATLLSHHSTAQPDLTLGLFPCTHPQSADMVALA
ADSCRVTAIEIKPAQTDLQWTWMAAIWEPTVTTLLGEVVAADRTARTQRQTCPELHIGAVFQAAIARGLRVEGVTFADGR
ALDIGTPAGWEAAAHFTG
>Mature_258_residues
MNTPVIGLIPAGGMATRLGPLPCSKELLPVGSFQTPDGLRPRPVITYLLAQWQRAGIGQALIVVRPGKWDIPAYLGDGSA
FGPRLAYLTVHEPHGAAYTLATALPFIQDRTVVLGLPDVILAPDDVYATLLSHHSTAQPDLTLGLFPCTHPQSADMVALA
ADSCRVTAIEIKPAQTDLQWTWMAAIWEPTVTTLLGEVVAADRTARTQRQTCPELHIGAVFQAAIARGLRVEGVTFADGR
ALDIGTPAGWEAAAHFTG

Specific function: Catalyzes The Formation Of Dtdp-Glucose, From Dttp And Glucose 1-Phosphate, As Well As Its Pyrophosphorolysis. [C]

COG id: COG1209

COG function: function code M; dTDP-glucose pyrophosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790224, Length=182, Percent_Identity=31.3186813186813, Blast_Score=61, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 2.7.7.24 [C]

Molecular weight: Translated: 27440; Mature: 27440

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTPVIGLIPAGGMATRLGPLPCSKELLPVGSFQTPDGLRPRPVITYLLAQWQRAGIGQA
CCCCEEEEECCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCE
LIVVRPGKWDIPAYLGDGSAFGPRLAYLTVHEPHGAAYTLATALPFIQDRTVVLGLPDVI
EEEEECCCCCCCEEECCCCCCCCEEEEEEEECCCCCEEEHHHHHHHHCCCEEEEECCCEE
LAPDDVYATLLSHHSTAQPDLTLGLFPCTHPQSADMVALAADSCRVTAIEIKPAQTDLQW
ECCCHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCEEEEECCCCEEEEEEEECCCCCCEE
TWMAAIWEPTVTTLLGEVVAADRTARTQRQTCPELHIGAVFQAAIARGLRVEGVTFADGR
EEEEEECCHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHCCCEECEEEECCCE
ALDIGTPAGWEAAAHFTG
EEECCCCCCCCCEECCCC
>Mature Secondary Structure
MNTPVIGLIPAGGMATRLGPLPCSKELLPVGSFQTPDGLRPRPVITYLLAQWQRAGIGQA
CCCCEEEEECCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCE
LIVVRPGKWDIPAYLGDGSAFGPRLAYLTVHEPHGAAYTLATALPFIQDRTVVLGLPDVI
EEEEECCCCCCCEEECCCCCCCCEEEEEEEECCCCCEEEHHHHHHHHCCCEEEEECCCEE
LAPDDVYATLLSHHSTAQPDLTLGLFPCTHPQSADMVALAADSCRVTAIEIKPAQTDLQW
ECCCHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCEEEEECCCCEEEEEEEECCCCCCEE
TWMAAIWEPTVTTLLGEVVAADRTARTQRQTCPELHIGAVFQAAIARGLRVEGVTFADGR
EEEEEECCHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHCCCEECEEEECCCE
ALDIGTPAGWEAAAHFTG
EEECCCCCCCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dTTP; D-Glucose 1-phosphate; H+ [C]

Specific reaction: dTTP + D-Glucose 1-phosphate + H+ --> dTDPglucose + Diphosphate [C]

General reaction: Nucleotidyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA