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The map label for this gene is purA [H]

Identifier: 222525350

GI number: 222525350

Start: 2664921

End: 2666213

Strand: Direct

Name: purA [H]

Synonym: Chy400_2095

Alternate gene names: 222525350

Gene position: 2664921-2666213 (Clockwise)

Preceding gene: 222525349

Following gene: 222525355

Centisome position: 50.58

GC content: 55.07

Gene sequence:

>1293_bases
ATGCCTGTAGTTGCATTAATTGGCGCTCAATGGGGCGATGAGGGTAAAGGCCATCTGGTCGATCTGCTGGCTGCGCGGGC
GCGGCTGGTCATTCGCTACGGTGGCGGCAATAATGCCGGACATACGGTTGTCAACCATCTTGGTACCTTTAAACTGCATC
TGGTGCCTTCTGGCATCTTCGATCCGGGCATCGTCAATGTCATCGGCCCTGGCGTGGTGGTGAATCCGGAAGTACTCATC
AAAGAGATTGAGGAGCTTGAAGCACGGGGTGTTTCCACGGCTAAATTGTTTGTCAGTGATCGCGCGCATGTGATCATGCC
GTATCACATTCAGCTCGATCAGTTGCAAGAGGAAGCACGCGGTGAAGGGCGGATTGGTACCACCGGTCGTGGTATTGGCC
CGGCGTATGCCGACAAGATGAGCCGCTCCGGCATCCGAATGGGTGACTTGTTGCACGAAGAGACGCTGCTAAACCGGTTG
CGCACAGTACTGGAAGAGAAGAATCGACTGCTGACCAAACTCTACAATGCCCGTCCGCTCTCACTGCACGATACCTATTT
GACCTATCTGGAGTATGGGCGACGGCTTGCCAATCATATCACCGATGTTCATCCGATCATCCACCGGGCATTAGAGAAGG
ATCTGCCAGTACTACTAGAAGGTGCGCAGGGTGCGTTGCTCGATATTGATCACGGCACATATCCATTCGTGACTTCCTCA
CCTCCAGGTGCTGCCGGTGCATGTCAGGGTAGTGGTATTGGTCCGACGCATATCAATTCGGTGATCGGGGTGGTAAAAGC
CTACACAACCCGCGTCGGCGAAGGGCCGTTCCCTACTGAAGTTGATGGGTCGGTGGCTGATGTGCTGCGTCAGATCGGTA
CACCCTGGGCAGAAGTTGGCACCACAACCGGTCGGCTACGACGGGTAGGCTGGTTTGATGCGGTGATGGCGCGCTATGCA
GTGCAGGTAAACGGCGTCGATACCCTGGCAATCACGAAACTTGATGTGCTCGATGGCCTGGAGACACTCAAGATTTGTAC
AGGCTATAAACTGCACGACGCCCTGTTGGAATATCCCCCTACCACGACAGCGCTGTTTGATCAGATCGAACCGGTTTATG
AAGAACTGCCGGGGTGGAGTGAACCAACGCGGCATGTTCGTCGCTTTGCCGATTTACCCGAAGCTGCTCAGGCGTATGTG
GCGCGCATTTGCCAGTTGGTTGGTGCCCGCCTGGGCATTATTTCGGTTGGCCCAGGTCGCGACCAGACGATTATGGTGGC
TGATGTGTTTTGA

Upstream 100 bases:

>100_bases
GCGATCACGTTACCAGAGAATGTCGGGCGGAGTCGAAGAGGAAACGAGCCGGTGATCAGAATCTGACTCGTAGCAGAATC
AATCTTTCAGGAGAGAGGCG

Downstream 100 bases:

>100_bases
GGGGTGTCGTCGGGGTAAAAGGTTGTTGTGTACAGCTCGTGTCAAATCTGGTAGCAGAGAAAGCCGAATACACTATCCCC
ACTCTAGCGGGGTGTCTGAA

Product: adenylosuccinate synthase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase [H]

Number of amino acids: Translated: 430; Mature: 429

Protein sequence:

>430_residues
MPVVALIGAQWGDEGKGHLVDLLAARARLVIRYGGGNNAGHTVVNHLGTFKLHLVPSGIFDPGIVNVIGPGVVVNPEVLI
KEIEELEARGVSTAKLFVSDRAHVIMPYHIQLDQLQEEARGEGRIGTTGRGIGPAYADKMSRSGIRMGDLLHEETLLNRL
RTVLEEKNRLLTKLYNARPLSLHDTYLTYLEYGRRLANHITDVHPIIHRALEKDLPVLLEGAQGALLDIDHGTYPFVTSS
PPGAAGACQGSGIGPTHINSVIGVVKAYTTRVGEGPFPTEVDGSVADVLRQIGTPWAEVGTTTGRLRRVGWFDAVMARYA
VQVNGVDTLAITKLDVLDGLETLKICTGYKLHDALLEYPPTTTALFDQIEPVYEELPGWSEPTRHVRRFADLPEAAQAYV
ARICQLVGARLGIISVGPGRDQTIMVADVF

Sequences:

>Translated_430_residues
MPVVALIGAQWGDEGKGHLVDLLAARARLVIRYGGGNNAGHTVVNHLGTFKLHLVPSGIFDPGIVNVIGPGVVVNPEVLI
KEIEELEARGVSTAKLFVSDRAHVIMPYHIQLDQLQEEARGEGRIGTTGRGIGPAYADKMSRSGIRMGDLLHEETLLNRL
RTVLEEKNRLLTKLYNARPLSLHDTYLTYLEYGRRLANHITDVHPIIHRALEKDLPVLLEGAQGALLDIDHGTYPFVTSS
PPGAAGACQGSGIGPTHINSVIGVVKAYTTRVGEGPFPTEVDGSVADVLRQIGTPWAEVGTTTGRLRRVGWFDAVMARYA
VQVNGVDTLAITKLDVLDGLETLKICTGYKLHDALLEYPPTTTALFDQIEPVYEELPGWSEPTRHVRRFADLPEAAQAYV
ARICQLVGARLGIISVGPGRDQTIMVADVF
>Mature_429_residues
PVVALIGAQWGDEGKGHLVDLLAARARLVIRYGGGNNAGHTVVNHLGTFKLHLVPSGIFDPGIVNVIGPGVVVNPEVLIK
EIEELEARGVSTAKLFVSDRAHVIMPYHIQLDQLQEEARGEGRIGTTGRGIGPAYADKMSRSGIRMGDLLHEETLLNRLR
TVLEEKNRLLTKLYNARPLSLHDTYLTYLEYGRRLANHITDVHPIIHRALEKDLPVLLEGAQGALLDIDHGTYPFVTSSP
PGAAGACQGSGIGPTHINSVIGVVKAYTTRVGEGPFPTEVDGSVADVLRQIGTPWAEVGTTTGRLRRVGWFDAVMARYAV
QVNGVDTLAITKLDVLDGLETLKICTGYKLHDALLEYPPTTTALFDQIEPVYEELPGWSEPTRHVRRFADLPEAAQAYVA
RICQLVGARLGIISVGPGRDQTIMVADVF

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP [H]

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family [H]

Homologues:

Organism=Homo sapiens, GI22748717, Length=431, Percent_Identity=41.7633410672854, Blast_Score=310, Evalue=2e-84,
Organism=Homo sapiens, GI34577063, Length=432, Percent_Identity=40.0462962962963, Blast_Score=301, Evalue=6e-82,
Organism=Homo sapiens, GI40316944, Length=399, Percent_Identity=40.6015037593985, Blast_Score=271, Evalue=7e-73,
Organism=Escherichia coli, GI1790620, Length=429, Percent_Identity=45.6876456876457, Blast_Score=384, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI25146553, Length=432, Percent_Identity=42.1296296296296, Blast_Score=322, Evalue=2e-88,
Organism=Caenorhabditis elegans, GI25146555, Length=432, Percent_Identity=42.1296296296296, Blast_Score=322, Evalue=3e-88,
Organism=Saccharomyces cerevisiae, GI6324109, Length=434, Percent_Identity=40.3225806451613, Blast_Score=317, Evalue=2e-87,
Organism=Drosophila melanogaster, GI21356033, Length=428, Percent_Identity=41.8224299065421, Blast_Score=322, Evalue=3e-88,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018220
- InterPro:   IPR001114 [H]

Pfam domain/function: PF00709 Adenylsucc_synt [H]

EC number: =6.3.4.4 [H]

Molecular weight: Translated: 46678; Mature: 46547

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1 ; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVVALIGAQWGDEGKGHLVDLLAARARLVIRYGGGNNAGHTVVNHLGTFKLHLVPSGIF
CCEEEEEECCCCCCCCCHHHHHHHHHCEEEEEECCCCCCHHHHHHHCCCEEEEEEECCCC
DPGIVNVIGPGVVVNPEVLIKEIEELEARGVSTAKLFVSDRAHVIMPYHIQLDQLQEEAR
CCCCHHHCCCCEEECHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEEECCHHHHHHHHC
GEGRIGTTGRGIGPAYADKMSRSGIRMGDLLHEETLLNRLRTVLEEKNRLLTKLYNARPL
CCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
SLHDTYLTYLEYGRRLANHITDVHPIIHRALEKDLPVLLEGAQGALLDIDHGTYPFVTSS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEECCCCCCCEEECC
PPGAAGACQGSGIGPTHINSVIGVVKAYTTRVGEGPFPTEVDGSVADVLRQIGTPWAEVG
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCHHHHC
TTTGRLRRVGWFDAVMARYAVQVNGVDTLAITKLDVLDGLETLKICTGYKLHDALLEYPP
CCHHHHHHHHHHHHHHHHHHEEECCCCEEEEEHHHHHHCHHHHHHHCCCHHHHHHHHCCC
TTTALFDQIEPVYEELPGWSEPTRHVRRFADLPEAAQAYVARICQLVGARLGIISVGPGR
CHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEECCCC
DQTIMVADVF
CCEEEEEECC
>Mature Secondary Structure 
PVVALIGAQWGDEGKGHLVDLLAARARLVIRYGGGNNAGHTVVNHLGTFKLHLVPSGIF
CEEEEEECCCCCCCCCHHHHHHHHHCEEEEEECCCCCCHHHHHHHCCCEEEEEEECCCC
DPGIVNVIGPGVVVNPEVLIKEIEELEARGVSTAKLFVSDRAHVIMPYHIQLDQLQEEAR
CCCCHHHCCCCEEECHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEEECCHHHHHHHHC
GEGRIGTTGRGIGPAYADKMSRSGIRMGDLLHEETLLNRLRTVLEEKNRLLTKLYNARPL
CCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
SLHDTYLTYLEYGRRLANHITDVHPIIHRALEKDLPVLLEGAQGALLDIDHGTYPFVTSS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEECCCCCCCEEECC
PPGAAGACQGSGIGPTHINSVIGVVKAYTTRVGEGPFPTEVDGSVADVLRQIGTPWAEVG
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCHHHHC
TTTGRLRRVGWFDAVMARYAVQVNGVDTLAITKLDVLDGLETLKICTGYKLHDALLEYPP
CCHHHHHHHHHHHHHHHHHHEEECCCCEEEEEHHHHHHCHHHHHHHCCCHHHHHHHHCCC
TTTALFDQIEPVYEELPGWSEPTRHVRRFADLPEAAQAYVARICQLVGARLGIISVGPGR
CHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEECCCC
DQTIMVADVF
CCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA