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The map label for this gene is alkK [H]

Identifier: 222525298

GI number: 222525298

Start: 2586479

End: 2588125

Strand: Direct

Name: alkK [H]

Synonym: Chy400_2040

Alternate gene names: 222525298

Gene position: 2586479-2588125 (Clockwise)

Preceding gene: 222525296

Following gene: 222525299

Centisome position: 49.09

GC content: 55.13

Gene sequence:

>1647_bases
ATGCACATGTATGAGCTTGGCCTGGACAAGAACGCAGCAAATTACACGCCCCTGACCCCGCTCTCGTTTCTTAAACGAAC
TGCGATGGTCTATCCCAATTTGCCGGCAGTTATTCACGGTGAACGGTGGTATACGTGGGCACAGGTCTACGAACGATCAC
GACGGCTTGCTTCAGCCCTGCGTGCGCTCGGTGTCGGCTTCCGCGATACGGTCGCTGTCGTTCTCAGCAACACACCCGAA
ATGTACGAGTGTCATTTCGGTGTCCCAGGAGCTGGTGCTGTTCTCAATACCATCAATGTCCGGCTTGATGCCGCAACGAT
TGCTTTCATCCTCGACCACGGTGAAGCGAAAGTTTTGATTACCGACCGCGAATTTTCGCCGGTGGTGAAAGCAGCGTTGG
AACAGTGTAACCGAACGTTGACCGTCATTGATGTAGACGATCCTTTGTACACCGGCCCCGGTGAGCGGTTGGGTAGTCTT
GAATACGAGCAGTTTCTCGCCAGTGGCGATCCTGAATTTGACTTGATCTATCCTGCCGATGAGTGGGAAGCAATTACGCT
TAACTACACGTCAGGCACAACGGCCAATCCGAAAGGTGTTGTCTATCACCATCGTGGGGCTTATCTCAATGCCCTCTCCA
ATATTGTTTCGTGGGGGATGCCCCATCACGCCGTCTACCTCTGGACGCTGCCGATGTTCCACTGCAACGGCTGGTGTTTT
CCCTGGACGATAGCTGCGAACGCGGGTACCAACATTTGTCTCCGTAAAGTTGATGCCGGGTTGATCTGGCAGGCGATTGA
AACGTACCGTGTCACTCATTACTGCGGTGCCCCGATTGTTCATTCGTTGATCGCCAATAATGCCCCTCCGCACTGGCGCG
AAGGCCGGGGCACCCATAAGGTCAGCGGTCTGATTGCCGCCGCGCCACCGCCGGCAGCCGTTTTGCAGGCAATGGCCGAA
ATTGGCTTTGATATTACCCATGTCTATGGCCTGACCGAAACCTACGGGCCGGCAGCGGTATGTGCAAAGCAACCCGACTG
GCATACGCTCCCGATTGCCGAACAGGCGCATCTCAACGGACGCCAGGGGGTAACCTACCATGCGCAAGAGGCGATTAGTG
TGCTTGATCCGGCGACGATGCAGCCGGTGCCGTGGGATGGGCAGACGATGGGTGAAGTGATGTTTCGCGGCAATATCGTT
ATGAAGGGCTATCTCAAGAATCCAGCCGCGACCGAAGCTGCTTTCCGCGATGGCTGGTTCCACTCTGGTGATCTGGCGGT
AGTACATCCAGATGGCTACATCAAGATCACCGACCGTGCAAAAGACATCATTATCTCTGGTGGCGAGAATATTTCTTCGA
TTGAGGTGGAAGATGCTCTCTACAAGCATCCGGCAGTGATGCTGGCTGCGGTGGTCGCTGCGCCCGATCCGAAGTGGGGC
GAGGTACCACATGCGTTTATCGAGCTAAGGGAAGGGGTTACCGTCACCGAGGAGGAGTTACAGCAGCACTGTCGGCGTTT
TCTGGCCGGTTACAAGATTCCCAAGAAGTTTACCTTCGGCCCCTTGCCGAAGACGTCAACCGGTAAGATTCAGAAGTTTA
TTTTGCGCGAACAGGCCCGGTCACGACAGGCTATCGCCGAAGCGTAG

Upstream 100 bases:

>100_bases
TTCCGCGACTGTATCACATTAGACGCCAATGCATGGCAGGGAGTCTGCGCCTGATGCAGACCTCAGCAGGTGTGGCAAAC
CGCACGAGGAGGTGTGTCCG

Downstream 100 bases:

>100_bases
TTTTGTATCTAATCGTGACTGACACTAGTGCGCTGCGATACACCCTCACCCTGACCCGTCCCCGCTGGGCTATGCATTAC
CCACATGTTGCGCTGCGTTG

Product: acyl-CoA synthetase

Products: D-carnitinyl-CoA; crotonobetainyl-CoA; L-carnitinyl-CoA [C]

Alternate protein names: Medium-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 548; Mature: 548

Protein sequence:

>548_residues
MHMYELGLDKNAANYTPLTPLSFLKRTAMVYPNLPAVIHGERWYTWAQVYERSRRLASALRALGVGFRDTVAVVLSNTPE
MYECHFGVPGAGAVLNTINVRLDAATIAFILDHGEAKVLITDREFSPVVKAALEQCNRTLTVIDVDDPLYTGPGERLGSL
EYEQFLASGDPEFDLIYPADEWEAITLNYTSGTTANPKGVVYHHRGAYLNALSNIVSWGMPHHAVYLWTLPMFHCNGWCF
PWTIAANAGTNICLRKVDAGLIWQAIETYRVTHYCGAPIVHSLIANNAPPHWREGRGTHKVSGLIAAAPPPAAVLQAMAE
IGFDITHVYGLTETYGPAAVCAKQPDWHTLPIAEQAHLNGRQGVTYHAQEAISVLDPATMQPVPWDGQTMGEVMFRGNIV
MKGYLKNPAATEAAFRDGWFHSGDLAVVHPDGYIKITDRAKDIIISGGENISSIEVEDALYKHPAVMLAAVVAAPDPKWG
EVPHAFIELREGVTVTEEELQQHCRRFLAGYKIPKKFTFGPLPKTSTGKIQKFILREQARSRQAIAEA

Sequences:

>Translated_548_residues
MHMYELGLDKNAANYTPLTPLSFLKRTAMVYPNLPAVIHGERWYTWAQVYERSRRLASALRALGVGFRDTVAVVLSNTPE
MYECHFGVPGAGAVLNTINVRLDAATIAFILDHGEAKVLITDREFSPVVKAALEQCNRTLTVIDVDDPLYTGPGERLGSL
EYEQFLASGDPEFDLIYPADEWEAITLNYTSGTTANPKGVVYHHRGAYLNALSNIVSWGMPHHAVYLWTLPMFHCNGWCF
PWTIAANAGTNICLRKVDAGLIWQAIETYRVTHYCGAPIVHSLIANNAPPHWREGRGTHKVSGLIAAAPPPAAVLQAMAE
IGFDITHVYGLTETYGPAAVCAKQPDWHTLPIAEQAHLNGRQGVTYHAQEAISVLDPATMQPVPWDGQTMGEVMFRGNIV
MKGYLKNPAATEAAFRDGWFHSGDLAVVHPDGYIKITDRAKDIIISGGENISSIEVEDALYKHPAVMLAAVVAAPDPKWG
EVPHAFIELREGVTVTEEELQQHCRRFLAGYKIPKKFTFGPLPKTSTGKIQKFILREQARSRQAIAEA
>Mature_548_residues
MHMYELGLDKNAANYTPLTPLSFLKRTAMVYPNLPAVIHGERWYTWAQVYERSRRLASALRALGVGFRDTVAVVLSNTPE
MYECHFGVPGAGAVLNTINVRLDAATIAFILDHGEAKVLITDREFSPVVKAALEQCNRTLTVIDVDDPLYTGPGERLGSL
EYEQFLASGDPEFDLIYPADEWEAITLNYTSGTTANPKGVVYHHRGAYLNALSNIVSWGMPHHAVYLWTLPMFHCNGWCF
PWTIAANAGTNICLRKVDAGLIWQAIETYRVTHYCGAPIVHSLIANNAPPHWREGRGTHKVSGLIAAAPPPAAVLQAMAE
IGFDITHVYGLTETYGPAAVCAKQPDWHTLPIAEQAHLNGRQGVTYHAQEAISVLDPATMQPVPWDGQTMGEVMFRGNIV
MKGYLKNPAATEAAFRDGWFHSGDLAVVHPDGYIKITDRAKDIIISGGENISSIEVEDALYKHPAVMLAAVVAAPDPKWG
EVPHAFIELREGVTVTEEELQQHCRRFLAGYKIPKKFTFGPLPKTSTGKIQKFILREQARSRQAIAEA

Specific function: Could Catalyze The Transfer Of CoA To Crotonobetaine Or Carnitine. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=571, Percent_Identity=29.5971978984238, Blast_Score=174, Evalue=2e-43,
Organism=Homo sapiens, GI157311624, Length=524, Percent_Identity=27.2900763358779, Blast_Score=152, Evalue=1e-36,
Organism=Homo sapiens, GI157311622, Length=524, Percent_Identity=27.2900763358779, Blast_Score=152, Evalue=1e-36,
Organism=Homo sapiens, GI58082049, Length=514, Percent_Identity=27.431906614786, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI38505220, Length=510, Percent_Identity=27.0588235294118, Blast_Score=146, Evalue=6e-35,
Organism=Homo sapiens, GI122937307, Length=531, Percent_Identity=26.5536723163842, Blast_Score=145, Evalue=7e-35,
Organism=Homo sapiens, GI42544132, Length=514, Percent_Identity=26.0700389105058, Blast_Score=137, Evalue=3e-32,
Organism=Homo sapiens, GI187761345, Length=570, Percent_Identity=25.4385964912281, Blast_Score=130, Evalue=3e-30,
Organism=Homo sapiens, GI187761343, Length=570, Percent_Identity=25.4385964912281, Blast_Score=130, Evalue=3e-30,
Organism=Homo sapiens, GI115511026, Length=519, Percent_Identity=26.2042389210019, Blast_Score=124, Evalue=3e-28,
Organism=Homo sapiens, GI8923896, Length=568, Percent_Identity=23.943661971831, Blast_Score=97, Evalue=5e-20,
Organism=Homo sapiens, GI28416953, Length=543, Percent_Identity=23.0202578268877, Blast_Score=96, Evalue=8e-20,
Organism=Homo sapiens, GI253970489, Length=395, Percent_Identity=27.3417721518987, Blast_Score=95, Evalue=2e-19,
Organism=Homo sapiens, GI40807491, Length=489, Percent_Identity=25.3578732106339, Blast_Score=80, Evalue=6e-15,
Organism=Escherichia coli, GI221142682, Length=514, Percent_Identity=26.2645914396887, Blast_Score=163, Evalue=2e-41,
Organism=Escherichia coli, GI1788107, Length=551, Percent_Identity=25.7713248638838, Blast_Score=157, Evalue=2e-39,
Organism=Escherichia coli, GI145693145, Length=538, Percent_Identity=29.182156133829, Blast_Score=141, Evalue=1e-34,
Organism=Escherichia coli, GI1786810, Length=536, Percent_Identity=25.7462686567164, Blast_Score=131, Evalue=1e-31,
Organism=Escherichia coli, GI1790505, Length=131, Percent_Identity=33.587786259542, Blast_Score=65, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI71994690, Length=535, Percent_Identity=28.5981308411215, Blast_Score=167, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI71994694, Length=535, Percent_Identity=28.5981308411215, Blast_Score=167, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI71994703, Length=535, Percent_Identity=28.5981308411215, Blast_Score=167, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI17558820, Length=514, Percent_Identity=28.5992217898833, Blast_Score=142, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI17560308, Length=529, Percent_Identity=27.0321361058601, Blast_Score=139, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI17559526, Length=519, Percent_Identity=24.8554913294798, Blast_Score=139, Evalue=5e-33,
Organism=Caenorhabditis elegans, GI17557194, Length=521, Percent_Identity=25.1439539347409, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI17538037, Length=510, Percent_Identity=26.078431372549, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI32563687, Length=528, Percent_Identity=25.3787878787879, Blast_Score=125, Evalue=7e-29,
Organism=Caenorhabditis elegans, GI17560140, Length=520, Percent_Identity=23.0769230769231, Blast_Score=109, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI17531443, Length=469, Percent_Identity=22.3880597014925, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI71983001, Length=544, Percent_Identity=25.1838235294118, Blast_Score=86, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI71982997, Length=544, Percent_Identity=25.1838235294118, Blast_Score=86, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI71996755, Length=515, Percent_Identity=23.3009708737864, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI115534440, Length=535, Percent_Identity=24.4859813084112, Blast_Score=80, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6319699, Length=359, Percent_Identity=28.6908077994429, Blast_Score=130, Evalue=5e-31,
Organism=Saccharomyces cerevisiae, GI6319264, Length=592, Percent_Identity=24.3243243243243, Blast_Score=88, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6323903, Length=393, Percent_Identity=24.1730279898219, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24581924, Length=565, Percent_Identity=27.4336283185841, Blast_Score=138, Evalue=1e-32,
Organism=Drosophila melanogaster, GI21355181, Length=534, Percent_Identity=26.4044943820225, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI18859661, Length=546, Percent_Identity=26.3736263736264, Blast_Score=116, Evalue=4e-26,
Organism=Drosophila melanogaster, GI19921316, Length=522, Percent_Identity=24.3295019157088, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI17933690, Length=544, Percent_Identity=23.1617647058824, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI62472339, Length=561, Percent_Identity=23.7076648841355, Blast_Score=85, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24667955, Length=560, Percent_Identity=23.75, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24667959, Length=393, Percent_Identity=25.4452926208651, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI281365686, Length=488, Percent_Identity=22.9508196721311, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI20130357, Length=160, Percent_Identity=28.75, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI21356947, Length=529, Percent_Identity=21.7391304347826, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI21358303, Length=129, Percent_Identity=30.2325581395349, Blast_Score=67, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.3.2.- [C]

Molecular weight: Translated: 60445; Mature: 60445

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHMYELGLDKNAANYTPLTPLSFLKRTAMVYPNLPAVIHGERWYTWAQVYERSRRLASAL
CCEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHH
RALGVGFRDTVAVVLSNTPEMYECHFGVPGAGAVLNTINVRLDAATIAFILDHGEAKVLI
HHHCCCHHHEEEEEECCCCCEEEEECCCCCCCCEEEEEEEEEEEEEEEEEEECCCEEEEE
TDREFSPVVKAALEQCNRTLTVIDVDDPLYTGPGERLGSLEYEQFLASGDPEFDLIYPAD
ECCCCCHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHCCCHHHHHHHCCCCCEEEEECCC
EWEAITLNYTSGTTANPKGVVYHHRGAYLNALSNIVSWGMPHHAVYLWTLPMFHCNGWCF
CCEEEEEEECCCCCCCCCCEEEECCCHHHHHHHHHHHCCCCCCEEEEEEEHHHHCCCEEE
PWTIAANAGTNICLRKVDAGLIWQAIETYRVTHYCGAPIVHSLIANNAPPHWREGRGTHK
EEEEECCCCCCEEEEHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCEE
VSGLIAAAPPPAAVLQAMAEIGFDITHVYGLTETYGPAAVCAKQPDWHTLPIAEQAHLNG
CCEEEECCCCHHHHHHHHHHCCCCEEEEEECCCCCCCHHEECCCCCCCCCCCCHHHCCCC
RQGVTYHAQEAISVLDPATMQPVPWDGQTMGEVMFRGNIVMKGYLKNPAATEAAFRDGWF
CCCCEEEHHHHHHHCCCCCCCCCCCCCCHHHHHEEECCEEEEEECCCCCHHHHHHHCCCE
HSGDLAVVHPDGYIKITDRAKDIIISGGENISSIEVEDALYKHPAVMLAAVVAAPDPKWG
ECCCEEEECCCCEEEEECCCCEEEEECCCCCCEEEHHHHHHHCHHHHHHHHHHCCCCCCC
EVPHAFIELREGVTVTEEELQQHCRRFLAGYKIPKKFTFGPLPKTSTGKIQKFILREQAR
CCCHHHHHHHCCCEECHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
SRQAIAEA
HHHHHHCC
>Mature Secondary Structure
MHMYELGLDKNAANYTPLTPLSFLKRTAMVYPNLPAVIHGERWYTWAQVYERSRRLASAL
CCEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHH
RALGVGFRDTVAVVLSNTPEMYECHFGVPGAGAVLNTINVRLDAATIAFILDHGEAKVLI
HHHCCCHHHEEEEEECCCCCEEEEECCCCCCCCEEEEEEEEEEEEEEEEEEECCCEEEEE
TDREFSPVVKAALEQCNRTLTVIDVDDPLYTGPGERLGSLEYEQFLASGDPEFDLIYPAD
ECCCCCHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHCCCHHHHHHHCCCCCEEEEECCC
EWEAITLNYTSGTTANPKGVVYHHRGAYLNALSNIVSWGMPHHAVYLWTLPMFHCNGWCF
CCEEEEEEECCCCCCCCCCEEEECCCHHHHHHHHHHHCCCCCCEEEEEEEHHHHCCCEEE
PWTIAANAGTNICLRKVDAGLIWQAIETYRVTHYCGAPIVHSLIANNAPPHWREGRGTHK
EEEEECCCCCCEEEEHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCEE
VSGLIAAAPPPAAVLQAMAEIGFDITHVYGLTETYGPAAVCAKQPDWHTLPIAEQAHLNG
CCEEEECCCCHHHHHHHHHHCCCCEEEEEECCCCCCCHHEECCCCCCCCCCCCHHHCCCC
RQGVTYHAQEAISVLDPATMQPVPWDGQTMGEVMFRGNIVMKGYLKNPAATEAAFRDGWF
CCCCEEEHHHHHHHCCCCCCCCCCCCCCHHHHHEEECCEEEEEECCCCCHHHHHHHCCCE
HSGDLAVVHPDGYIKITDRAKDIIISGGENISSIEVEDALYKHPAVMLAAVVAAPDPKWG
ECCCEEEECCCCEEEEECCCCEEEEECCCCCCEEEHHHHHHHCHHHHHHHHHHCCCCCCC
EVPHAFIELREGVTVTEEELQQHCRRFLAGYKIPKKFTFGPLPKTSTGKIQKFILREQAR
CCCHHHHHHHCCCEECHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
SRQAIAEA
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Coenzyme A; D-carnitine; Coenzyme A; crotono-betaine; L-carnitine [C]

Specific reaction: Coenzyme A + D-carnitine = D-carnitinyl-CoA Coenzyme A + crotono-betaine = crotonobetainyl-CoA Coenzyme A + L-carnitine = L-carnitinyl-CoA [C]

General reaction: Ligases; Forming Carbon-Sulfur Bonds; Acid-Thiol Ligases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1453953 [H]