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The map label for this gene is metB [H]

Identifier: 222525287

GI number: 222525287

Start: 2567961

End: 2569082

Strand: Reverse

Name: metB [H]

Synonym: Chy400_2028

Alternate gene names: 222525287

Gene position: 2569082-2567961 (Counterclockwise)

Preceding gene: 222525289

Following gene: 222525285

Centisome position: 48.76

GC content: 59.45

Gene sequence:

>1122_bases
ATGGCACGACATTCTCAGCATCTCAAACCAGAGTCATGGCTGGTAGCAGCCGGACGCACGATTGAAGCCGGTGCGCCGCT
CAATGTTCCGCCAATTCCGGCATCCAATTTTCTTATCGGTCAAGGGCGTTCATATGCCCGAAATGATGCAACGCCAACCT
GGGAAGCCGTCGAAGAGATTGTTGGTGGTCTTGAAGGCGGACGAGCAGTGGCGTTTGCGTCGGGAATGGCGGCGATTGCT
GCCGTGTTTGATCAATTGCCCGCCGGTGCACAGGTTGTGTTACCGGACGATTGTTACCAGGGTGTGACCGGGCTAGCAAT
GGCCGGGGAAGCGCGTGGACGCTGGTCAGTGCGCCGGTTGGCGCTTACCGATACCGAAGGCTGGCAGCAAGCCTGTGCCG
TGGCCGATCTGGTCTGGCTTGAGTCGCCTTCAAATCCGTTGCTCGTCGTTGCCGATCTCCCGGCAATCTGTGCTGCGCCG
CGCAAGCCCGGTACCATCGTCGCGGTAGACAATACCTTCGCCACCTCGTTGAACCAGCAGCCACTCGAGTTTGGGGCAAC
CCTCTCCATTCAATCGGCCACCAAGTTTATCGGCGGACATTCCGACTTGCTCGCAGGTGTGGTAACGACTCGTGATGAAC
AACTCTGGCACGATTTGCGACGGTCGCGTGAATTGACCGGAGCCACCCCTGGCGCCTTCGAGGCATTTCTGGCGGCGCGT
GGTGCGCGCACGCTGGCATTGCGGTTGGAACGGGCGCAGCACAATGCGATGGAGCTGGCCCAACGGCTGTCCCACCACCC
GCTGGTTAGCTGTGTCCGTTATCCGGGTTTGCCAACGCATCCAACCCATGCCATTGCCTGTCGCGTATTGAAGGGGTTCG
GAACCATTATCTCGTTCGACCTGCGCGGTGATGCCACATTTGCCGATGCAGTCTGCCGCAATGTCAGCCTGATCCGCCAT
GCAACCAGCCTGGGAGCTGTTGAGTCAACTATGGAGCGACGGGCTGCGATTGCCGGGCAAGAGCATCTGCCACCCACGCT
ACTGCGGCTTAGCGTTGGGATTGAAGATGTTGATGATCTCTGGGCAGATCTCGATGGAGCGATCCGACGGGCCGCACAGT
GA

Upstream 100 bases:

>100_bases
TTGACACTCTTCGTCGTCAATGTGATAATCACCCGCACAACCGTAGTCTCCTGGGCATGGCCTTCTCTTACAAGAGCCGC
CTCTGAACGTAAGGTGTAGT

Downstream 100 bases:

>100_bases
GGATCAACCGGACGACCGGCTGCGTTCGGTACGTAAATGTGCGGCCAACGCGGCGACTGGCGGAAACTCTGTTGCCCACT
CGTCATCGAGCGGCCAGAAT

Product: Cys/Met metabolism pyridoxal-phosphate-dependent protein

Products: NA

Alternate protein names: CGS; O-succinylhomoserine (thiol)-lyase [H]

Number of amino acids: Translated: 373; Mature: 372

Protein sequence:

>373_residues
MARHSQHLKPESWLVAAGRTIEAGAPLNVPPIPASNFLIGQGRSYARNDATPTWEAVEEIVGGLEGGRAVAFASGMAAIA
AVFDQLPAGAQVVLPDDCYQGVTGLAMAGEARGRWSVRRLALTDTEGWQQACAVADLVWLESPSNPLLVVADLPAICAAP
RKPGTIVAVDNTFATSLNQQPLEFGATLSIQSATKFIGGHSDLLAGVVTTRDEQLWHDLRRSRELTGATPGAFEAFLAAR
GARTLALRLERAQHNAMELAQRLSHHPLVSCVRYPGLPTHPTHAIACRVLKGFGTIISFDLRGDATFADAVCRNVSLIRH
ATSLGAVESTMERRAAIAGQEHLPPTLLRLSVGIEDVDDLWADLDGAIRRAAQ

Sequences:

>Translated_373_residues
MARHSQHLKPESWLVAAGRTIEAGAPLNVPPIPASNFLIGQGRSYARNDATPTWEAVEEIVGGLEGGRAVAFASGMAAIA
AVFDQLPAGAQVVLPDDCYQGVTGLAMAGEARGRWSVRRLALTDTEGWQQACAVADLVWLESPSNPLLVVADLPAICAAP
RKPGTIVAVDNTFATSLNQQPLEFGATLSIQSATKFIGGHSDLLAGVVTTRDEQLWHDLRRSRELTGATPGAFEAFLAAR
GARTLALRLERAQHNAMELAQRLSHHPLVSCVRYPGLPTHPTHAIACRVLKGFGTIISFDLRGDATFADAVCRNVSLIRH
ATSLGAVESTMERRAAIAGQEHLPPTLLRLSVGIEDVDDLWADLDGAIRRAAQ
>Mature_372_residues
ARHSQHLKPESWLVAAGRTIEAGAPLNVPPIPASNFLIGQGRSYARNDATPTWEAVEEIVGGLEGGRAVAFASGMAAIAA
VFDQLPAGAQVVLPDDCYQGVTGLAMAGEARGRWSVRRLALTDTEGWQQACAVADLVWLESPSNPLLVVADLPAICAAPR
KPGTIVAVDNTFATSLNQQPLEFGATLSIQSATKFIGGHSDLLAGVVTTRDEQLWHDLRRSRELTGATPGAFEAFLAARG
ARTLALRLERAQHNAMELAQRLSHHPLVSCVRYPGLPTHPTHAIACRVLKGFGTIISFDLRGDATFADAVCRNVSLIRHA
TSLGAVESTMERRAAIAGQEHLPPTLLRLSVGIEDVDDLWADLDGAIRRAAQ

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=343, Percent_Identity=34.402332361516, Blast_Score=177, Evalue=1e-44,
Organism=Homo sapiens, GI299473759, Length=227, Percent_Identity=37.8854625550661, Blast_Score=149, Evalue=3e-36,
Organism=Homo sapiens, GI25453487, Length=341, Percent_Identity=31.3782991202346, Blast_Score=135, Evalue=5e-32,
Organism=Escherichia coli, GI1790375, Length=365, Percent_Identity=38.9041095890411, Blast_Score=202, Evalue=4e-53,
Organism=Escherichia coli, GI1789383, Length=357, Percent_Identity=31.9327731092437, Blast_Score=142, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI71980708, Length=342, Percent_Identity=35.672514619883, Blast_Score=209, Evalue=2e-54,
Organism=Caenorhabditis elegans, GI17560056, Length=325, Percent_Identity=34.1538461538462, Blast_Score=187, Evalue=1e-47,
Organism=Caenorhabditis elegans, GI17538055, Length=348, Percent_Identity=33.0459770114943, Blast_Score=178, Evalue=5e-45,
Organism=Saccharomyces cerevisiae, GI6319307, Length=349, Percent_Identity=34.0974212034384, Blast_Score=169, Evalue=5e-43,
Organism=Saccharomyces cerevisiae, GI6321904, Length=330, Percent_Identity=29.6969696969697, Blast_Score=140, Evalue=4e-34,
Organism=Saccharomyces cerevisiae, GI6321254, Length=387, Percent_Identity=27.6485788113695, Blast_Score=118, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6321170, Length=273, Percent_Identity=30.4029304029304, Blast_Score=105, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6323333, Length=290, Percent_Identity=31.0344827586207, Blast_Score=94, Evalue=2e-20,
Organism=Drosophila melanogaster, GI19922554, Length=363, Percent_Identity=33.0578512396694, Blast_Score=199, Evalue=2e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =2.5.1.48 [H]

Molecular weight: Translated: 39756; Mature: 39624

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARHSQHLKPESWLVAAGRTIEAGAPLNVPPIPASNFLIGQGRSYARNDATPTWEAVEEI
CCCCCCCCCCCCEEEECCCEEECCCCCCCCCCCCCCEEEECCCCCCCCCCCCCHHHHHHH
VGGLEGGRAVAFASGMAAIAAVFDQLPAGAQVVLPDDCYQGVTGLAMAGEARGRWSVRRL
HCCCCCCCEEEHHHHHHHHHHHHHHCCCCCEEEECCHHHCCCHHHHCCCCCCCCEEEEEE
ALTDTEGWQQACAVADLVWLESPSNPLLVVADLPAICAAPRKPGTIVAVDNTFATSLNQQ
EECCCHHHHHHHHHHHHHEEECCCCCEEEEECCCHHHCCCCCCCEEEEECCHHHHCCCCC
PLEFGATLSIQSATKFIGGHSDLLAGVVTTRDEQLWHDLRRSRELTGATPGAFEAFLAAR
CHHHCCEEEEHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHC
GARTLALRLERAQHNAMELAQRLSHHPLVSCVRYPGLPTHPTHAIACRVLKGFGTIISFD
CCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCHHHHHHHHHHHCCCEEEEE
LRGDATFADAVCRNVSLIRHATSLGAVESTMERRAAIAGQEHLPPTLLRLSVGIEDVDDL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHEEEECCCCHHHHH
WADLDGAIRRAAQ
HHHHHHHHHHHCC
>Mature Secondary Structure 
ARHSQHLKPESWLVAAGRTIEAGAPLNVPPIPASNFLIGQGRSYARNDATPTWEAVEEI
CCCCCCCCCCCEEEECCCEEECCCCCCCCCCCCCCEEEECCCCCCCCCCCCCHHHHHHH
VGGLEGGRAVAFASGMAAIAAVFDQLPAGAQVVLPDDCYQGVTGLAMAGEARGRWSVRRL
HCCCCCCCEEEHHHHHHHHHHHHHHCCCCCEEEECCHHHCCCHHHHCCCCCCCCEEEEEE
ALTDTEGWQQACAVADLVWLESPSNPLLVVADLPAICAAPRKPGTIVAVDNTFATSLNQQ
EECCCHHHHHHHHHHHHHEEECCCCCEEEEECCCHHHCCCCCCCEEEEECCHHHHCCCCC
PLEFGATLSIQSATKFIGGHSDLLAGVVTTRDEQLWHDLRRSRELTGATPGAFEAFLAAR
CHHHCCEEEEHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHC
GARTLALRLERAQHNAMELAQRLSHHPLVSCVRYPGLPTHPTHAIACRVLKGFGTIISFD
CCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCHHHHHHHHHHHCCCEEEEE
LRGDATFADAVCRNVSLIRHATSLGAVESTMERRAAIAGQEHLPPTLLRLSVGIEDVDDL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHEEEECCCCHHHHH
WADLDGAIRRAAQ
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]