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The map label for this gene is proS [H]

Identifier: 222525199

GI number: 222525199

Start: 2460124

End: 2461701

Strand: Reverse

Name: proS [H]

Synonym: Chy400_1939

Alternate gene names: 222525199

Gene position: 2461701-2460124 (Counterclockwise)

Preceding gene: 222525200

Following gene: 222525197

Centisome position: 46.72

GC content: 64.51

Gene sequence:

>1578_bases
ATGATACAACTTGCCACGCTGTTTGGGCGAACGTTACGCCAGGCGCCCGCAGAAGCTGATACGCGGGCAGCCCAACTACT
CTGGCGCGCCGGGATGATCCGCGCCCTGGATAACGGTGAACCGGCCCTGTTACCCCTGGGGAGTGCGGTGCTGCGCCGGC
TCAGCCACCATCTGACCACAGCGATCCAGGCTGGCGGTGCTCAAGAGGTGTATCTGCCAAACACATCCACAGCCACCTGG
CCGGCGACCGTGACTGCGCTGGCCCGGCGTGAGATCGACTCATACCGCCAGCTTCCCCAGGCGATCTGGGGTCAGCGTCG
GCGCAAACGACCGCAATCCCGTCAGCGCTTCAGCCTGCTCGAACTGCCCACACCCACGACGTTGAGCTGGGCCGGACTGG
CGCGCGACGCCTCGCAGGCCCAGGCGTTGCTTGAAACGACGCTGCAACACCTGCGCCACGCCTTGCACGAGTGTGGGCTA
ACGATTGTCGCCGATGCCTTGCAGGCCACCGAATCTGCGCCGCTGATCGTTGACAGTGCGAGTGGGCCGTTAACAACCCT
CGATTGCCCCACCTGCAACCGGCGAGCACCGCGCGAACTGGCGCCAATGGCGCCACCACCGGCCTACAGCGGGCCGACTC
CGGCGATGAGCCTGGTCGAAACCCCCAACGCCAACACGATTGCTGCCCTGGCAGCGTACCTCGGTATTCCCACCGCAGCG
ACGGCAAAAGCGCTCTTCTTCGATGCCCGTTGTGCCGACGACCGGCGCCTCGTGTTCGCCGTGGTGCGTGGCGACCGGGA
AGCAAGTCTACCCAAACTGGCTGCCGTTGTCGGTGCCGACGAGCTGGTTCCTGCCGACGAAGCGCAGATTCGAGCGTGCG
GAGCCGTTCCCGGCTATGCGTCACCCGTCGGCCTGCGCGATGTGCTCGTCGTTGCCGACCCAATGGTCACCACTGGGGCG
CCACTGGTGGCGGGCGCAAACCGGGCTGGCTACCATCTGCGCGATGTGGTCTATGGCCGTGACTGGCAGGCCACCCTGGT
CGCCGACATTAGCCAGGCCGAGGCCGGCGATCCATGCCCATGGTGTGGAACGGCCCGGATTGAAGGGCGGGGTGCGCTTA
TCGGCGCTATAACAGCCCCTCAACCAACTGCGCTGCTCGTGCAAGATGCAGAGGGCCAAAACCGGCCACTCATCATTACG
GGTCTCGAAATCGATCTGGAACCGCTCCTGTACCTGGTGGTTGAGCAGCATCACGACGAACGCGGGATTGTCTGGCCGGC
GGCCATTGCCCCCTTCGCCATCCACCTCGTCACCCTGGGGCGCGGCGAGGCAGTAGTTACCGCCGGACACGCACTGGCGG
CGATGCTGCAAACGGCTGGTTGGCATGTGTTGATTGATGATCGTGATGAACGGGCCGGAGTTAAATTCAACGATGCCGAC
CTGATCGGCGCCCCGTGGCGCGTCGTCATCAGTGAGAAGCTACTGGCCGCGAATCAGCTTGAAATCCGACACCGTACCGC
CGCCCAGGCCACCGTCATCGATCAATCAGCCCTACTGACTGTGATCGGGCAGCGGTAG

Upstream 100 bases:

>100_bases
CCGCTGGCTGAGAGAGATAACGAACCGCCGACCACACCGGCTCAGCTCAACCTGTTTGGCTAAACCCTTCGCCTATGTGA
TGTGATCGAACGAGGACGAA

Downstream 100 bases:

>100_bases
GTGATCGACACCGACGCAGCCTATTGAGGCCCTAACATCTGCACCAGCCAGGCCCGCACTGCGGCGCGGGCCGCTTCCGA
TTCAGGCGAGCCATCGGTAT

Product: Proline--tRNA ligase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS [H]

Number of amino acids: Translated: 525; Mature: 525

Protein sequence:

>525_residues
MIQLATLFGRTLRQAPAEADTRAAQLLWRAGMIRALDNGEPALLPLGSAVLRRLSHHLTTAIQAGGAQEVYLPNTSTATW
PATVTALARREIDSYRQLPQAIWGQRRRKRPQSRQRFSLLELPTPTTLSWAGLARDASQAQALLETTLQHLRHALHECGL
TIVADALQATESAPLIVDSASGPLTTLDCPTCNRRAPRELAPMAPPPAYSGPTPAMSLVETPNANTIAALAAYLGIPTAA
TAKALFFDARCADDRRLVFAVVRGDREASLPKLAAVVGADELVPADEAQIRACGAVPGYASPVGLRDVLVVADPMVTTGA
PLVAGANRAGYHLRDVVYGRDWQATLVADISQAEAGDPCPWCGTARIEGRGALIGAITAPQPTALLVQDAEGQNRPLIIT
GLEIDLEPLLYLVVEQHHDERGIVWPAAIAPFAIHLVTLGRGEAVVTAGHALAAMLQTAGWHVLIDDRDERAGVKFNDAD
LIGAPWRVVISEKLLAANQLEIRHRTAAQATVIDQSALLTVIGQR

Sequences:

>Translated_525_residues
MIQLATLFGRTLRQAPAEADTRAAQLLWRAGMIRALDNGEPALLPLGSAVLRRLSHHLTTAIQAGGAQEVYLPNTSTATW
PATVTALARREIDSYRQLPQAIWGQRRRKRPQSRQRFSLLELPTPTTLSWAGLARDASQAQALLETTLQHLRHALHECGL
TIVADALQATESAPLIVDSASGPLTTLDCPTCNRRAPRELAPMAPPPAYSGPTPAMSLVETPNANTIAALAAYLGIPTAA
TAKALFFDARCADDRRLVFAVVRGDREASLPKLAAVVGADELVPADEAQIRACGAVPGYASPVGLRDVLVVADPMVTTGA
PLVAGANRAGYHLRDVVYGRDWQATLVADISQAEAGDPCPWCGTARIEGRGALIGAITAPQPTALLVQDAEGQNRPLIIT
GLEIDLEPLLYLVVEQHHDERGIVWPAAIAPFAIHLVTLGRGEAVVTAGHALAAMLQTAGWHVLIDDRDERAGVKFNDAD
LIGAPWRVVISEKLLAANQLEIRHRTAAQATVIDQSALLTVIGQR
>Mature_525_residues
MIQLATLFGRTLRQAPAEADTRAAQLLWRAGMIRALDNGEPALLPLGSAVLRRLSHHLTTAIQAGGAQEVYLPNTSTATW
PATVTALARREIDSYRQLPQAIWGQRRRKRPQSRQRFSLLELPTPTTLSWAGLARDASQAQALLETTLQHLRHALHECGL
TIVADALQATESAPLIVDSASGPLTTLDCPTCNRRAPRELAPMAPPPAYSGPTPAMSLVETPNANTIAALAAYLGIPTAA
TAKALFFDARCADDRRLVFAVVRGDREASLPKLAAVVGADELVPADEAQIRACGAVPGYASPVGLRDVLVVADPMVTTGA
PLVAGANRAGYHLRDVVYGRDWQATLVADISQAEAGDPCPWCGTARIEGRGALIGAITAPQPTALLVQDAEGQNRPLIIT
GLEIDLEPLLYLVVEQHHDERGIVWPAAIAPFAIHLVTLGRGEAVVTAGHALAAMLQTAGWHVLIDDRDERAGVKFNDAD
LIGAPWRVVISEKLLAANQLEIRHRTAAQATVIDQSALLTVIGQR

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786392, Length=562, Percent_Identity=30.2491103202847, Blast_Score=191, Evalue=8e-50,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK [H]

EC number: =6.1.1.15 [H]

Molecular weight: Translated: 56005; Mature: 56005

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQLATLFGRTLRQAPAEADTRAAQLLWRAGMIRALDNGEPALLPLGSAVLRRLSHHLTT
CEEEHHHHHHHHHHCCCCHHHHHHHHHHHHHHHEEECCCCCCEEECCHHHHHHHHHHHHH
AIQAGGAQEVYLPNTSTATWPATVTALARREIDSYRQLPQAIWGQRRRKRPQSRQRFSLL
HHHCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHEEEE
ELPTPTTLSWAGLARDASQAQALLETTLQHLRHALHECGLTIVADALQATESAPLIVDSA
ECCCCCCEEHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCEEEECC
SGPLTTLDCPTCNRRAPRELAPMAPPPAYSGPTPAMSLVETPNANTIAALAAYLGIPTAA
CCCEEEECCCCCCCCCCHHHCCCCCCCCCCCCCCCHHEEECCCCCHHHHHHHHHCCCCHH
TAKALFFDARCADDRRLVFAVVRGDREASLPKLAAVVGADELVPADEAQIRACGAVPGYA
HHHHHEEECCCCCCCEEEEEEEECCCCCCCHHHHHHHCCCCCCCCCCHHEEEECCCCCCC
SPVGLRDVLVVADPMVTTGAPLVAGANRAGYHLRDVVYGRDWQATLVADISQAEAGDPCP
CCCCCCEEEEEECCCCCCCCCCEECCCCCCCCHHHEEECCCCCEEEEEECHHCCCCCCCC
WCGTARIEGRGALIGAITAPQPTALLVQDAEGQNRPLIITGLEIDLEPLLYLVVEQHHDE
CCCCEEECCCCEEEEEECCCCCCEEEEECCCCCCCCEEEEECEECHHHHHHHHHHHCCCC
RGIVWPAAIAPFAIHLVTLGRGEAVVTAGHALAAMLQTAGWHVLIDDRDERAGVKFNDAD
CCCEEHHHHHHHEEEEEEECCCCEEEECHHHHHHHHHHCCCEEEEECCCCCCCCEECCCC
LIGAPWRVVISEKLLAANQLEIRHRTAAQATVIDQSALLTVIGQR
CCCCCHHHHHHHHHHHCCHHHHHHHHHHHHEEECCHHHHHEECCC
>Mature Secondary Structure
MIQLATLFGRTLRQAPAEADTRAAQLLWRAGMIRALDNGEPALLPLGSAVLRRLSHHLTT
CEEEHHHHHHHHHHCCCCHHHHHHHHHHHHHHHEEECCCCCCEEECCHHHHHHHHHHHHH
AIQAGGAQEVYLPNTSTATWPATVTALARREIDSYRQLPQAIWGQRRRKRPQSRQRFSLL
HHHCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHEEEE
ELPTPTTLSWAGLARDASQAQALLETTLQHLRHALHECGLTIVADALQATESAPLIVDSA
ECCCCCCEEHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCEEEECC
SGPLTTLDCPTCNRRAPRELAPMAPPPAYSGPTPAMSLVETPNANTIAALAAYLGIPTAA
CCCEEEECCCCCCCCCCHHHCCCCCCCCCCCCCCCHHEEECCCCCHHHHHHHHHCCCCHH
TAKALFFDARCADDRRLVFAVVRGDREASLPKLAAVVGADELVPADEAQIRACGAVPGYA
HHHHHEEECCCCCCCEEEEEEEECCCCCCCHHHHHHHCCCCCCCCCCHHEEEECCCCCCC
SPVGLRDVLVVADPMVTTGAPLVAGANRAGYHLRDVVYGRDWQATLVADISQAEAGDPCP
CCCCCCEEEEEECCCCCCCCCCEECCCCCCCCHHHEEECCCCCEEEEEECHHCCCCCCCC
WCGTARIEGRGALIGAITAPQPTALLVQDAEGQNRPLIITGLEIDLEPLLYLVVEQHHDE
CCCCEEECCCCEEEEEECCCCCCEEEEECCCCCCCCEEEEECEECHHHHHHHHHHHCCCC
RGIVWPAAIAPFAIHLVTLGRGEAVVTAGHALAAMLQTAGWHVLIDDRDERAGVKFNDAD
CCCEEHHHHHHHEEEEEEECCCCEEEECHHHHHHHHHHCCCEEEEECCCCCCCCEECCCC
LIGAPWRVVISEKLLAANQLEIRHRTAAQATVIDQSALLTVIGQR
CCCCCHHHHHHHHHHHCCHHHHHHHHHHHHEEECCHHHHHEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA