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The map label for this gene is rfbE [H]

Identifier: 222525151

GI number: 222525151

Start: 2390231

End: 2391259

Strand: Direct

Name: rfbE [H]

Synonym: Chy400_1889

Alternate gene names: 222525151

Gene position: 2390231-2391259 (Clockwise)

Preceding gene: 222525150

Following gene: 222525152

Centisome position: 45.36

GC content: 56.17

Gene sequence:

>1029_bases
ATGAAAACATCGTTCCACCTCATCACCGGCGGTGCCGGGTTCATCGGTTGTAATCTGGCTGATTATTTGCTGGCCCGTGG
CGAACACGTCACGATTGTTGATAATCTGAGTCGTCCACGAACCCCGCTCAATCTGGCGTGGTTGCAGGAACGGTACGGTA
GCCGGCTTCGCTTCGTGCAGGCCGATATTCGTGATGCCGCGGTGATGCAGGCTGTGATCCCCGGTCATCGGGTCGTTTAC
CATCTTGCCGGTCAGGTGGCAGTGACGACATCGGTGCAGGATCCGCGCAGTGATTTTGAAATTAATGCGCTCGGCACGCT
GAATATCCTCGAAGCAGCCCGGCTGGCGAGCGAGCCACCGATTGTCTTTTTTGCCTCTACCAACAAGGTCTACGGCGGGA
TGGAGACCGTTGCCGTAGTGGAAGATGAGACTCGCTACCGTTACCGTGATTTGCCGCATGGAGTACCTGAACATCAGTTA
CTCGATTTTCATTCACCGTATGGGTGCAGCAAAGGTGCTGCCGATCAATATGTGCGTGATTATGCCCGTATCTACGGTTT
GAAAACGGTGGTCTTCCGCCAGTCGTGCATCTATGGCCCGCGCCAGTTTGGGGTTGAGGATCAGGGGTGGGCTGCGCACT
TCGCGATTGCCGCACTCTTGAACCGTCCCATTACCATCTACGGCAACGGGAAGCAGGTGCGCGATATGCTCTACGTTGAT
GATCTGATCGCAGCCTATATGGCGGCACTGGACCGGATCGATCAGGTGAGTGGACGGATATATAATATCGGTGGTGGTCC
GCAGAATGCACTTTCCATCTGGGCTGAATTTGGCCCACTGCTGAGCCGGCTGATCGGACGAGAGATTCCGGTGCGTTATG
GCGACTGGCGACCCGGCGATCAGCCGGTCTACATCAGTGATATTTCGCTGGCGCAGCGCGATCTGGCCTGGCAACCACAG
GTGTCGGTGTACGAAGGGGTGGAACGGATGGTGGGCTGGATTCGCGATCATCTCGATCTGTTCCGTTAG

Upstream 100 bases:

>100_bases
AATCGGTCGGCTGATTTGCCGACCGATTCATTGTATCGTTATAATGATGAAGCCTGAATTGAAATTACGAAGGAGCAGGC
TGTACGATACTATAAACGCT

Downstream 100 bases:

>100_bases
CGATGTCTGATCTACTCTCTGTAAGCGAAACGTGAGTGGTTATGAGTATATCAAGGCCGGTCATAGTTCATCCACAAGAC
GATGAGCCGTTCGATGTGGC

Product: NAD-dependent epimerase/dehydratase

Products: NA

Alternate protein names: CDP-tyvelose 2-epimerase [H]

Number of amino acids: Translated: 342; Mature: 342

Protein sequence:

>342_residues
MKTSFHLITGGAGFIGCNLADYLLARGEHVTIVDNLSRPRTPLNLAWLQERYGSRLRFVQADIRDAAVMQAVIPGHRVVY
HLAGQVAVTTSVQDPRSDFEINALGTLNILEAARLASEPPIVFFASTNKVYGGMETVAVVEDETRYRYRDLPHGVPEHQL
LDFHSPYGCSKGAADQYVRDYARIYGLKTVVFRQSCIYGPRQFGVEDQGWAAHFAIAALLNRPITIYGNGKQVRDMLYVD
DLIAAYMAALDRIDQVSGRIYNIGGGPQNALSIWAEFGPLLSRLIGREIPVRYGDWRPGDQPVYISDISLAQRDLAWQPQ
VSVYEGVERMVGWIRDHLDLFR

Sequences:

>Translated_342_residues
MKTSFHLITGGAGFIGCNLADYLLARGEHVTIVDNLSRPRTPLNLAWLQERYGSRLRFVQADIRDAAVMQAVIPGHRVVY
HLAGQVAVTTSVQDPRSDFEINALGTLNILEAARLASEPPIVFFASTNKVYGGMETVAVVEDETRYRYRDLPHGVPEHQL
LDFHSPYGCSKGAADQYVRDYARIYGLKTVVFRQSCIYGPRQFGVEDQGWAAHFAIAALLNRPITIYGNGKQVRDMLYVD
DLIAAYMAALDRIDQVSGRIYNIGGGPQNALSIWAEFGPLLSRLIGREIPVRYGDWRPGDQPVYISDISLAQRDLAWQPQ
VSVYEGVERMVGWIRDHLDLFR
>Mature_342_residues
MKTSFHLITGGAGFIGCNLADYLLARGEHVTIVDNLSRPRTPLNLAWLQERYGSRLRFVQADIRDAAVMQAVIPGHRVVY
HLAGQVAVTTSVQDPRSDFEINALGTLNILEAARLASEPPIVFFASTNKVYGGMETVAVVEDETRYRYRDLPHGVPEHQL
LDFHSPYGCSKGAADQYVRDYARIYGLKTVVFRQSCIYGPRQFGVEDQGWAAHFAIAALLNRPITIYGNGKQVRDMLYVD
DLIAAYMAALDRIDQVSGRIYNIGGGPQNALSIWAEFGPLLSRLIGREIPVRYGDWRPGDQPVYISDISLAQRDLAWQPQ
VSVYEGVERMVGWIRDHLDLFR

Specific function: Catalyzes the isomeration of CDP-paratose to CDP- tyvelose [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family [H]

Homologues:

Organism=Homo sapiens, GI42516563, Length=336, Percent_Identity=27.6785714285714, Blast_Score=112, Evalue=6e-25,
Organism=Homo sapiens, GI7657641, Length=343, Percent_Identity=26.530612244898, Blast_Score=102, Evalue=3e-22,
Organism=Homo sapiens, GI56237023, Length=358, Percent_Identity=25.9776536312849, Blast_Score=99, Evalue=5e-21,
Organism=Homo sapiens, GI56118217, Length=358, Percent_Identity=25.9776536312849, Blast_Score=99, Evalue=5e-21,
Organism=Homo sapiens, GI189083684, Length=358, Percent_Identity=25.9776536312849, Blast_Score=99, Evalue=5e-21,
Organism=Escherichia coli, GI48994969, Length=350, Percent_Identity=31.7142857142857, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI1788353, Length=343, Percent_Identity=31.7784256559767, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI1786974, Length=350, Percent_Identity=28.8571428571429, Blast_Score=118, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI17539532, Length=339, Percent_Identity=26.5486725663717, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI115532424, Length=340, Percent_Identity=28.2352941176471, Blast_Score=99, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI71982035, Length=354, Percent_Identity=26.8361581920904, Blast_Score=98, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI71982038, Length=356, Percent_Identity=26.685393258427, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17568069, Length=343, Percent_Identity=25.3644314868805, Blast_Score=94, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6319493, Length=355, Percent_Identity=24.7887323943662, Blast_Score=84, Evalue=3e-17,
Organism=Drosophila melanogaster, GI21356223, Length=336, Percent_Identity=27.6785714285714, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI19923002, Length=352, Percent_Identity=26.7045454545455, Blast_Score=92, Evalue=5e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =5.1.3.10 [H]

Molecular weight: Translated: 38343; Mature: 38343

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTSFHLITGGAGFIGCNLADYLLARGEHVTIVDNLSRPRTPLNLAWLQERYGSRLRFVQ
CCCEEEEEECCCCEECCCHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHCCCEEEEH
ADIRDAAVMQAVIPGHRVVYHLAGQVAVTTSVQDPRSDFEINALGTLNILEAARLASEPP
HHHHHHHHHHHHCCCHHEEEEECCCEEEEECCCCCCCCEEEEEECHHHHHHHHHHCCCCC
IVFFASTNKVYGGMETVAVVEDETRYRYRDLPHGVPEHQLLDFHSPYGCSKGAADQYVRD
EEEEECCCCCCCCCEEEEEEECCHHHHHHCCCCCCCCCCEECCCCCCCCCCCHHHHHHHH
YARIYGLKTVVFRQSCIYGPRQFGVEDQGWAAHFAIAALLNRPITIYGNGKQVRDMLYVD
HHHHHHHHHHHHHHHHCCCHHHCCCCCCCHHHHHHHHHHHCCCEEEEECCHHHHHHHHHH
DLIAAYMAALDRIDQVSGRIYNIGGGPQNALSIWAEFGPLLSRLIGREIPVRYGDWRPGD
HHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
QPVYISDISLAQRDLAWQPQVSVYEGVERMVGWIRDHLDLFR
CCEEEECCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKTSFHLITGGAGFIGCNLADYLLARGEHVTIVDNLSRPRTPLNLAWLQERYGSRLRFVQ
CCCEEEEEECCCCEECCCHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHCCCEEEEH
ADIRDAAVMQAVIPGHRVVYHLAGQVAVTTSVQDPRSDFEINALGTLNILEAARLASEPP
HHHHHHHHHHHHCCCHHEEEEECCCEEEEECCCCCCCCEEEEEECHHHHHHHHHHCCCCC
IVFFASTNKVYGGMETVAVVEDETRYRYRDLPHGVPEHQLLDFHSPYGCSKGAADQYVRD
EEEEECCCCCCCCCEEEEEEECCHHHHHHCCCCCCCCCCEECCCCCCCCCCCHHHHHHHH
YARIYGLKTVVFRQSCIYGPRQFGVEDQGWAAHFAIAALLNRPITIYGNGKQVRDMLYVD
HHHHHHHHHHHHHHHHCCCHHHCCCCCCCHHHHHHHHHHHCCCEEEEECCHHHHHHHHHH
DLIAAYMAALDRIDQVSGRIYNIGGGPQNALSIWAEFGPLLSRLIGREIPVRYGDWRPGD
HHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
QPVYISDISLAQRDLAWQPQVSVYEGVERMVGWIRDHLDLFR
CCEEEECCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2793833; 11677608; 12644504; 12642575 [H]