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The map label for this gene is gatA [H]

Identifier: 222525117

GI number: 222525117

Start: 2346261

End: 2347724

Strand: Direct

Name: gatA [H]

Synonym: Chy400_1855

Alternate gene names: 222525117

Gene position: 2346261-2347724 (Clockwise)

Preceding gene: 222525115

Following gene: 222525118

Centisome position: 44.53

GC content: 59.9

Gene sequence:

>1464_bases
ATGACTGAACTACACCATCTCACCGTCACTGCTGCACAAGCAGCACTCGCTGCCGGCGACATCACGGCGGTGGAACTTAC
TGAAGCCTGTCTGGCCCGGATCAACGCTACTGAACCGACAATCCGTGCCTTTCTTCATCTCACACCAGATGCAGCCCTGG
CTGCGGCACGGGCTGCCGATGAGCGGCGGCAACAAGGACGCTCACTTGGACCGCTCGACGGCATTCCCATCGCGATCAAA
GATGTTATCTGCACCGATGGCGTTCCGACGACTGCCGGCTCACGGATTCTGGCCGGTTTTCGCCCCCCGTACAATGCCAC
AGTGATCGAGCGCCTTGTTGCGGCGGGGGCCGTTCTGGTCGGTAAACTCAACTGCGATGAGTTTGCTATGGGTTCGAGCA
CCGAGAATAGTGCCTATCAGATTACCACCAACCCGTGGGACCCAACCAGGGTCCCAGGCGGGAGCAGTGGTGGTAGCGCG
GCAGCAGTCGCTGCCGGGCAGGTGCCGGCAACCCTGGGGACCGATACTGGCGGCTCGATTCGCCAACCGGCTGCGCTCTG
TGGGATCAGCGGTCTCAAGCCGACGTATGGCCGGGTCAGCCGTTACGGTCTCATTGCCTATGGATCGTCACTCGATCAAA
TCGGGCCAATGGCCTGGACAGTTGCCGATCTGGCGGTACTGCTCAACGTCATTGCCGGCCATGATCCACGTGATGGTACC
AGTGCTCCCATTGACACTCCCGATTACACCACAGCTCTGACCGGTGACATTCGCGGGTTGCGGATCGGTATTCCCCGTGA
ATATTTTGTTGAAGGGATGGAGCCTGGTGTCGAGTCGGCTACCCGTACCGCTATCGAGGTGTTGCGCGATCTGGGTGCGA
TCCTGGTCGATGTTTCGTTGCCACACACCAGATATGCGTTGCCCACCTACTACATCATTGCCCCCGCCGAAGCCAGTGCC
AACCTGGCCCGCTTCGACGGCGTGCGCTACGGCTTCCGGGCCGAGGGTGAAACGATGTGGGAGCAGATCGAACAGACCCG
TGGTCAGGGATTTGGTCCCGAAGTTCGGCGTCGCATCATGCTCGGTACCTATGCTCTCTCCGCAGGGTATTACGATGCCT
ATTATCGACGGGCCCAGCAGGTGCGCACGTTAATCAAGCGCGACTTTGAGCAGGTGTTTACGCAGGTCGACCTGTTGGCA
GCACCGACCTCGCCAACCGTTGCCTTTCCGATTGGTCAGAAGATTAACGATCCGCTGGCAATGTATCTGAGCGATGTCTG
TACGTTGCCGATCAACCTGGCCGGTGTACCGGCGCTGGTCGTCCCATGCGGTTTCAGTGAGGGGCTGCCGGTAGGCCTGC
AACTGATCGGACGCCCCTTCGATGAGGCCACGCTGCTACGGGTAGGTGATGCCTACCAGCGCCTGACCGAATGGCATACG
CAGCGTCCCAGGCTAGTGGTTTAG

Upstream 100 bases:

>100_bases
CGCAGATCGTCCTGTTCAGTAGCCTGATGGACCCTCTCTCTGCCTGGGCGAAGTCTGGTACAATAGAGCGACTATGCGCC
ACGACTCCACTGGAGGCGAT

Downstream 100 bases:

>100_bases
GCAGCCGGCGTCTTTGCGCACTGATCGGTCAAGATATGGTATACTAGAAGACGGCTTAAAACAAATATGTCGCGTCGAAA
GATATAAGGAGAGAGACAAT

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 487; Mature: 486

Protein sequence:

>487_residues
MTELHHLTVTAAQAALAAGDITAVELTEACLARINATEPTIRAFLHLTPDAALAAARAADERRQQGRSLGPLDGIPIAIK
DVICTDGVPTTAGSRILAGFRPPYNATVIERLVAAGAVLVGKLNCDEFAMGSSTENSAYQITTNPWDPTRVPGGSSGGSA
AAVAAGQVPATLGTDTGGSIRQPAALCGISGLKPTYGRVSRYGLIAYGSSLDQIGPMAWTVADLAVLLNVIAGHDPRDGT
SAPIDTPDYTTALTGDIRGLRIGIPREYFVEGMEPGVESATRTAIEVLRDLGAILVDVSLPHTRYALPTYYIIAPAEASA
NLARFDGVRYGFRAEGETMWEQIEQTRGQGFGPEVRRRIMLGTYALSAGYYDAYYRRAQQVRTLIKRDFEQVFTQVDLLA
APTSPTVAFPIGQKINDPLAMYLSDVCTLPINLAGVPALVVPCGFSEGLPVGLQLIGRPFDEATLLRVGDAYQRLTEWHT
QRPRLVV

Sequences:

>Translated_487_residues
MTELHHLTVTAAQAALAAGDITAVELTEACLARINATEPTIRAFLHLTPDAALAAARAADERRQQGRSLGPLDGIPIAIK
DVICTDGVPTTAGSRILAGFRPPYNATVIERLVAAGAVLVGKLNCDEFAMGSSTENSAYQITTNPWDPTRVPGGSSGGSA
AAVAAGQVPATLGTDTGGSIRQPAALCGISGLKPTYGRVSRYGLIAYGSSLDQIGPMAWTVADLAVLLNVIAGHDPRDGT
SAPIDTPDYTTALTGDIRGLRIGIPREYFVEGMEPGVESATRTAIEVLRDLGAILVDVSLPHTRYALPTYYIIAPAEASA
NLARFDGVRYGFRAEGETMWEQIEQTRGQGFGPEVRRRIMLGTYALSAGYYDAYYRRAQQVRTLIKRDFEQVFTQVDLLA
APTSPTVAFPIGQKINDPLAMYLSDVCTLPINLAGVPALVVPCGFSEGLPVGLQLIGRPFDEATLLRVGDAYQRLTEWHT
QRPRLVV
>Mature_486_residues
TELHHLTVTAAQAALAAGDITAVELTEACLARINATEPTIRAFLHLTPDAALAAARAADERRQQGRSLGPLDGIPIAIKD
VICTDGVPTTAGSRILAGFRPPYNATVIERLVAAGAVLVGKLNCDEFAMGSSTENSAYQITTNPWDPTRVPGGSSGGSAA
AVAAGQVPATLGTDTGGSIRQPAALCGISGLKPTYGRVSRYGLIAYGSSLDQIGPMAWTVADLAVLLNVIAGHDPRDGTS
APIDTPDYTTALTGDIRGLRIGIPREYFVEGMEPGVESATRTAIEVLRDLGAILVDVSLPHTRYALPTYYIIAPAEASAN
LARFDGVRYGFRAEGETMWEQIEQTRGQGFGPEVRRRIMLGTYALSAGYYDAYYRRAQQVRTLIKRDFEQVFTQVDLLAA
PTSPTVAFPIGQKINDPLAMYLSDVCTLPINLAGVPALVVPCGFSEGLPVGLQLIGRPFDEATLLRVGDAYQRLTEWHTQ
RPRLVV

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=492, Percent_Identity=38.8211382113821, Blast_Score=334, Evalue=1e-91,
Organism=Homo sapiens, GI195972892, Length=471, Percent_Identity=23.5668789808917, Blast_Score=125, Evalue=1e-28,
Organism=Homo sapiens, GI166795287, Length=153, Percent_Identity=37.9084967320261, Blast_Score=72, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17543272, Length=422, Percent_Identity=38.6255924170616, Blast_Score=256, Evalue=1e-68,
Organism=Caenorhabditis elegans, GI17537465, Length=492, Percent_Identity=25.2032520325203, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17556264, Length=294, Percent_Identity=30.6122448979592, Blast_Score=102, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI71990152, Length=243, Percent_Identity=32.0987654320988, Blast_Score=102, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17538252, Length=294, Percent_Identity=28.9115646258503, Blast_Score=85, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI17556276, Length=320, Percent_Identity=27.8125, Blast_Score=76, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17556278, Length=320, Percent_Identity=27.8125, Blast_Score=76, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17538254, Length=194, Percent_Identity=30.4123711340206, Blast_Score=68, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6323950, Length=417, Percent_Identity=36.6906474820144, Blast_Score=243, Evalue=4e-65,
Organism=Saccharomyces cerevisiae, GI6319685, Length=460, Percent_Identity=31.304347826087, Blast_Score=127, Evalue=5e-30,
Organism=Saccharomyces cerevisiae, GI6320448, Length=240, Percent_Identity=29.5833333333333, Blast_Score=76, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24648113, Length=448, Percent_Identity=41.9642857142857, Blast_Score=295, Evalue=6e-80,
Organism=Drosophila melanogaster, GI24644968, Length=488, Percent_Identity=26.4344262295082, Blast_Score=126, Evalue=4e-29,
Organism=Drosophila melanogaster, GI24652985, Length=481, Percent_Identity=27.027027027027, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI19922090, Length=481, Percent_Identity=27.027027027027, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24652981, Length=481, Percent_Identity=27.027027027027, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24652983, Length=481, Percent_Identity=27.027027027027, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI161078093, Length=515, Percent_Identity=27.1844660194175, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI45550774, Length=497, Percent_Identity=25.7545271629779, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24648435, Length=497, Percent_Identity=25.7545271629779, Blast_Score=102, Evalue=6e-22,
Organism=Drosophila melanogaster, GI24648437, Length=497, Percent_Identity=25.7545271629779, Blast_Score=102, Evalue=6e-22,
Organism=Drosophila melanogaster, GI21356731, Length=448, Percent_Identity=25.6696428571429, Blast_Score=99, Evalue=6e-21,
Organism=Drosophila melanogaster, GI24648441, Length=424, Percent_Identity=25.9433962264151, Blast_Score=91, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24648439, Length=424, Percent_Identity=25.9433962264151, Blast_Score=91, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 51879; Mature: 51747

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTELHHLTVTAAQAALAAGDITAVELTEACLARINATEPTIRAFLHLTPDAALAAARAAD
CCCCEEEEHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHEEEEEEECCHHHHHHHHHHH
ERRQQGRSLGPLDGIPIAIKDVICTDGVPTTAGSRILAGFRPPYNATVIERLVAAGAVLV
HHHHCCCCCCCCCCCCEEEEHEEECCCCCCCCCCCEEECCCCCCCHHHHHHHHHCCEEEE
GKLNCDEFAMGSSTENSAYQITTNPWDPTRVPGGSSGGSAAAVAAGQVPATLGTDTGGSI
EECCCCHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCC
RQPAALCGISGLKPTYGRVSRYGLIAYGSSLDQIGPMAWTVADLAVLLNVIAGHDPRDGT
CCCHHHHCCCCCCCCHHHHHHCEEEEECCCHHHHCCHHHHHHHHHHHHHHHHCCCCCCCC
SAPIDTPDYTTALTGDIRGLRIGIPREYFVEGMEPGVESATRTAIEVLRDLGAILVDVSL
CCCCCCCCCCEEEECCCCEEEECCCHHHHHCCCCCCHHHHHHHHHHHHHHCCCEEEEECC
PHTRYALPTYYIIAPAEASANLARFDGVRYGFRAEGETMWEQIEQTRGQGFGPEVRRRIM
CCCEEECCEEEEEECCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHH
LGTYALSAGYYDAYYRRAQQVRTLIKRDFEQVFTQVDLLAAPTSPTVAFPIGQKINDPLA
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEEEECCCCCCCHHH
MYLSDVCTLPINLAGVPALVVPCGFSEGLPVGLQLIGRPFDEATLLRVGDAYQRLTEWHT
HHHHHHHHCCCCCCCCCEEEEECCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHC
QRPRLVV
CCCCCCC
>Mature Secondary Structure 
TELHHLTVTAAQAALAAGDITAVELTEACLARINATEPTIRAFLHLTPDAALAAARAAD
CCCEEEEHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHEEEEEEECCHHHHHHHHHHH
ERRQQGRSLGPLDGIPIAIKDVICTDGVPTTAGSRILAGFRPPYNATVIERLVAAGAVLV
HHHHCCCCCCCCCCCCEEEEHEEECCCCCCCCCCCEEECCCCCCCHHHHHHHHHCCEEEE
GKLNCDEFAMGSSTENSAYQITTNPWDPTRVPGGSSGGSAAAVAAGQVPATLGTDTGGSI
EECCCCHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCC
RQPAALCGISGLKPTYGRVSRYGLIAYGSSLDQIGPMAWTVADLAVLLNVIAGHDPRDGT
CCCHHHHCCCCCCCCHHHHHHCEEEEECCCHHHHCCHHHHHHHHHHHHHHHHCCCCCCCC
SAPIDTPDYTTALTGDIRGLRIGIPREYFVEGMEPGVESATRTAIEVLRDLGAILVDVSL
CCCCCCCCCCEEEECCCCEEEECCCHHHHHCCCCCCHHHHHHHHHHHHHHCCCEEEEECC
PHTRYALPTYYIIAPAEASANLARFDGVRYGFRAEGETMWEQIEQTRGQGFGPEVRRRIM
CCCEEECCEEEEEECCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHH
LGTYALSAGYYDAYYRRAQQVRTLIKRDFEQVFTQVDLLAAPTSPTVAFPIGQKINDPLA
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEEEECCCCCCCHHH
MYLSDVCTLPINLAGVPALVVPCGFSEGLPVGLQLIGRPFDEATLLRVGDAYQRLTEWHT
HHHHHHHHCCCCCCCCCEEEEECCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHC
QRPRLVV
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA