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The map label for this gene is gdhA [H]

Identifier: 222525101

GI number: 222525101

Start: 2328407

End: 2329693

Strand: Direct

Name: gdhA [H]

Synonym: Chy400_1839

Alternate gene names: 222525101

Gene position: 2328407-2329693 (Clockwise)

Preceding gene: 222525100

Following gene: 222525102

Centisome position: 44.19

GC content: 58.43

Gene sequence:

>1287_bases
ATGGTGATGACAGATATTGAACGATCTGCCGCCGCGCCTTCTGAGAGCGTTGATCTGCTGAACATTGTGCAGCAGCATTT
TGATCAGGCGGCTGAACTGCTCGACCTTCCGGCACGTCTCCGCGGCATTTTACGAGTGCCGCAGCGCGAATTGACCGTCA
ACTTTCCGGTGAAGCGGGATAGTGGACGGATCGAGGTCTTTCAGGGCTTTCGTGTCCAGCACAATCTGGCCCGTGGCCCA
ACGAAAGGCGGCATTCGCTACCACCCCAATGTCACGCTTGACGAGACCCGCGCCCTGGCCATGTTGATGACCTGGAAGTG
TGCGCTGGCCGGTTTGCCATACGGCGGTGCGAAAGGTGCGGTGATTGTTGATCCCAAACAGCTTTCAGTCGGTGAAATCG
AACGCCTGACCCGGCGTTTTGCCACCGAGATTAGTGTTGTAATCGGCCCTGAGCGCGATATTCCGGCGCCCGATGTCGGT
ACCAACCCCCAGGTGATGGCGTGGATTATGGATACCATCTCGATGCACCAGGGCCATACCGTACCGGCAGTCGTAACCGG
GAAGCCGATCAATATCGGCGGCTCGGAAGGGCGGCGCGAGGCTACCGGTCGCGGATTAACCTACGTGTTGACGGCTGCGG
CCCACCATCTCGGTCTGAATATCTCGGATATCCGGCTGGCGATACAGGGGTGTGGCAATGTTGGCTCAACCGTGGCCCGC
GAAGCAGTCGCGCTGGGGATGAAGGTCATTGCCCTTAGTGACAGCCGTGGTGGAGTCTACAATCCGCACGGACTGGATAT
TGAGGCTATCCTGGCTCACAAAGCGCATACCGGCAGTGTGGTTGGTGCCGTCAATGCCGACTCACTGACCAACGAAGAAT
TGCTGGAAGTTGAATGCGATGTATTAGTGCCGGCAGCGTTGAGTGGTGTGATCACAGCCCAAAACGCCGGTCGGATCAGA
GCGCAGATCGTCGCTGAAGCGGCCAATGGCCCCACCACCAAAGCTGCCGATGCGATCCTGTATGATCGGGGATGCCTGGT
CATTCCAGACATTCTCGCCAATGCCGGTGGTGTCACCGTGAGCTACTTCGAGTGGGTGCAGGGTTTGCAAGAGTTTTTCT
GGAGCGAGCGGGAAGTCAACACGCAGTTGCGCCGAGTGATGACCAATGCGTTGCAACAGGTCTTGCGGGTCTCAGCCGAG
CGACAGGTCGATCTGCGCACGGCATCGTATATGCTGGCCGTGCAGCGCGTGGCCGATGCCGTCACGACCCGCGGTATTTA
TCCGTAG

Upstream 100 bases:

>100_bases
TATCAGCTATGGCACCGTGGTACAATAGGGGCTGTCGCGCCGGCGTTGTCGCGACCGGCCAGCGTTGGCCGATTCTACTC
ATCGTTGAGGGGTAAAGGTC

Downstream 100 bases:

>100_bases
CATGGGCAGGTTATGGACGAACTGGATGCAATGATCACCGACCTCGTTCCGCACCCGATCAGCGGAAGGCGGTTTCTCTT
CGGTCTCCTGTTAGGCTTCG

Product: Glu/Leu/Phe/Val dehydrogenase

Products: NA

Alternate protein names: GDH [H]

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MVMTDIERSAAAPSESVDLLNIVQQHFDQAAELLDLPARLRGILRVPQRELTVNFPVKRDSGRIEVFQGFRVQHNLARGP
TKGGIRYHPNVTLDETRALAMLMTWKCALAGLPYGGAKGAVIVDPKQLSVGEIERLTRRFATEISVVIGPERDIPAPDVG
TNPQVMAWIMDTISMHQGHTVPAVVTGKPINIGGSEGRREATGRGLTYVLTAAAHHLGLNISDIRLAIQGCGNVGSTVAR
EAVALGMKVIALSDSRGGVYNPHGLDIEAILAHKAHTGSVVGAVNADSLTNEELLEVECDVLVPAALSGVITAQNAGRIR
AQIVAEAANGPTTKAADAILYDRGCLVIPDILANAGGVTVSYFEWVQGLQEFFWSEREVNTQLRRVMTNALQQVLRVSAE
RQVDLRTASYMLAVQRVADAVTTRGIYP

Sequences:

>Translated_428_residues
MVMTDIERSAAAPSESVDLLNIVQQHFDQAAELLDLPARLRGILRVPQRELTVNFPVKRDSGRIEVFQGFRVQHNLARGP
TKGGIRYHPNVTLDETRALAMLMTWKCALAGLPYGGAKGAVIVDPKQLSVGEIERLTRRFATEISVVIGPERDIPAPDVG
TNPQVMAWIMDTISMHQGHTVPAVVTGKPINIGGSEGRREATGRGLTYVLTAAAHHLGLNISDIRLAIQGCGNVGSTVAR
EAVALGMKVIALSDSRGGVYNPHGLDIEAILAHKAHTGSVVGAVNADSLTNEELLEVECDVLVPAALSGVITAQNAGRIR
AQIVAEAANGPTTKAADAILYDRGCLVIPDILANAGGVTVSYFEWVQGLQEFFWSEREVNTQLRRVMTNALQQVLRVSAE
RQVDLRTASYMLAVQRVADAVTTRGIYP
>Mature_428_residues
MVMTDIERSAAAPSESVDLLNIVQQHFDQAAELLDLPARLRGILRVPQRELTVNFPVKRDSGRIEVFQGFRVQHNLARGP
TKGGIRYHPNVTLDETRALAMLMTWKCALAGLPYGGAKGAVIVDPKQLSVGEIERLTRRFATEISVVIGPERDIPAPDVG
TNPQVMAWIMDTISMHQGHTVPAVVTGKPINIGGSEGRREATGRGLTYVLTAAAHHLGLNISDIRLAIQGCGNVGSTVAR
EAVALGMKVIALSDSRGGVYNPHGLDIEAILAHKAHTGSVVGAVNADSLTNEELLEVECDVLVPAALSGVITAQNAGRIR
AQIVAEAANGPTTKAADAILYDRGCLVIPDILANAGGVTVSYFEWVQGLQEFFWSEREVNTQLRRVMTNALQQVLRVSAE
RQVDLRTASYMLAVQRVADAVTTRGIYP

Specific function: Unknown

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=413, Percent_Identity=40.6779661016949, Blast_Score=297, Evalue=1e-80,
Organism=Homo sapiens, GI4885281, Length=413, Percent_Identity=40.1937046004843, Blast_Score=294, Evalue=9e-80,
Organism=Escherichia coli, GI1788059, Length=394, Percent_Identity=30.7106598984772, Blast_Score=172, Evalue=4e-44,
Organism=Caenorhabditis elegans, GI17544676, Length=384, Percent_Identity=43.2291666666667, Blast_Score=286, Evalue=2e-77,
Organism=Saccharomyces cerevisiae, GI6324951, Length=418, Percent_Identity=30.622009569378, Blast_Score=167, Evalue=4e-42,
Organism=Saccharomyces cerevisiae, GI6319256, Length=400, Percent_Identity=31.75, Blast_Score=162, Evalue=7e-41,
Organism=Drosophila melanogaster, GI45549226, Length=380, Percent_Identity=42.1052631578947, Blast_Score=297, Evalue=8e-81,
Organism=Drosophila melanogaster, GI45553475, Length=378, Percent_Identity=42.5925925925926, Blast_Score=297, Evalue=1e-80,
Organism=Drosophila melanogaster, GI24649283, Length=366, Percent_Identity=37.7049180327869, Blast_Score=255, Evalue=4e-68,
Organism=Drosophila melanogaster, GI24649460, Length=313, Percent_Identity=42.4920127795527, Blast_Score=241, Evalue=6e-64,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR014362
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.3 [H]

Molecular weight: Translated: 46035; Mature: 46035

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVMTDIERSAAAPSESVDLLNIVQQHFDQAAELLDLPARLRGILRVPQRELTVNFPVKRD
CCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCEEEECCEECC
SGRIEVFQGFRVQHNLARGPTKGGIRYHPNVTLDETRALAMLMTWKCALAGLPYGGAKGA
CCCEEEEECCCHHHHHCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCE
VIVDPKQLSVGEIERLTRRFATEISVVIGPERDIPAPDVGTNPQVMAWIMDTISMHQGHT
EEECCCCCCHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCC
VPAVVTGKPINIGGSEGRREATGRGLTYVLTAAAHHLGLNISDIRLAIQGCGNVGSTVAR
CCEEEECCEEECCCCCCCCHHCCCCHHHHHHHHHHHHCCCHHHHHEEEECCCCCHHHHHH
EAVALGMKVIALSDSRGGVYNPHGLDIEAILAHKAHTGSVVGAVNADSLTNEELLEVECD
HHHHHCEEEEEEECCCCCCCCCCCCCHHHHHHCCCCCCCEEEECCCCCCCCCHHHEEECC
VLVPAALSGVITAQNAGRIRAQIVAEAANGPTTKAADAILYDRGCLVIPDILANAGGVTV
EEEHHHHCCEEEECCCCCEEEEEEEECCCCCCCHHHHHEEECCCCEEECHHHCCCCCCEE
SYFEWVQGLQEFFWSEREVNTQLRRVMTNALQQVLRVSAERQVDLRTASYMLAVQRVADA
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
VTTRGIYP
HHHCCCCC
>Mature Secondary Structure
MVMTDIERSAAAPSESVDLLNIVQQHFDQAAELLDLPARLRGILRVPQRELTVNFPVKRD
CCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCEEEECCEECC
SGRIEVFQGFRVQHNLARGPTKGGIRYHPNVTLDETRALAMLMTWKCALAGLPYGGAKGA
CCCEEEEECCCHHHHHCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCE
VIVDPKQLSVGEIERLTRRFATEISVVIGPERDIPAPDVGTNPQVMAWIMDTISMHQGHT
EEECCCCCCHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCC
VPAVVTGKPINIGGSEGRREATGRGLTYVLTAAAHHLGLNISDIRLAIQGCGNVGSTVAR
CCEEEECCEEECCCCCCCCHHCCCCHHHHHHHHHHHHCCCHHHHHEEEECCCCCHHHHHH
EAVALGMKVIALSDSRGGVYNPHGLDIEAILAHKAHTGSVVGAVNADSLTNEELLEVECD
HHHHHCEEEEEEECCCCCCCCCCCCCHHHHHHCCCCCCCEEEECCCCCCCCCHHHEEECC
VLVPAALSGVITAQNAGRIRAQIVAEAANGPTTKAADAILYDRGCLVIPDILANAGGVTV
EEEHHHHCCEEEECCCCCEEEEEEEECCCCCCCHHHHHEEECCCCEEECHHHCCCCCCEE
SYFEWVQGLQEFFWSEREVNTQLRRVMTNALQQVLRVSAERQVDLRTASYMLAVQRVADA
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
VTTRGIYP
HHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9680336; 10360571; 9135121; 9654452; 10366510 [H]