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The map label for this gene is potD [H]

Identifier: 222525088

GI number: 222525088

Start: 2307871

End: 2309022

Strand: Direct

Name: potD [H]

Synonym: Chy400_1825

Alternate gene names: 222525088

Gene position: 2307871-2309022 (Clockwise)

Preceding gene: 222525087

Following gene: 222525089

Centisome position: 43.8

GC content: 53.3

Gene sequence:

>1152_bases
ATGCAGATGCTTCGATTGCTGGTGCTGGTGCTGCTTCTGATACTAACGGCGTGTGGTGGCGGTGGTGCAACCACCAGTCC
CGGTAACGAATACGGTACCGGCAGCGGTGATACAACGCAGACAACCGGTGATGGTGTTGATCGCAGCAGATTGTCAGATA
CGCTCTACTTCTACAACTGGTCAGATTATATCGATCCGGCGATCCTCGACCAGTTCAAGGCCGAGTATGGCGTTGAAGTC
GTGGTTGACACCTACGACAGCAACGAAGACATGCTGGCAAAGGTGCGGGCCGGTAATTCGGGGTACGATATTGTCGTTCC
CTCGGATTATGCGGTGCAGATCATGGTGGCAGAGGGATTAGCTGCACCGATAGACAAGTCCTTGCTCACTAATCTGGTGC
ATATCGATCCCAACCTGCTAGATCAGTACTTCGACAAAGGAAATGCCTATTCGGTGCCGTACATGTATGGCATTACCGGC
ATTGCCTACAACACGAAATTCTTCCCGAACGGCATCGATAGCTGGGCTGCGATCTTTGAACCCGATCAGATTGCTCAGTT
TGCCGGCAAGTTTAGCATGCTCGATGATGCGCGTGAGACTCCGGGGGCGGCGCTACGCTATATCGGTCAGTCACTCAATT
CCACCGATCCGGCGGCATTAGAGCGGGTTAAGGAGATTCTTCTGACCCAGAAGCAATACCTGGCAGCCTATAACAGTTCT
GACGTGAACCGCAAACTTGCCAGCGAGGAATACGTTATGGCCCATGCCTGGAGCGGAACGGCTATGCAGGCGCGCAATGG
GTTGGGTGACGAGTTCAGCGGTAATCCGAACATCGCCTTTGTGATCCCCAAAGAGGGTGGCATGATCTGGATGGACAATC
TCGTTATCCTCAAGGACTCACCCAATGCCTATACGGCCCACGTCTTCATCAATTTTCTCATGCGACCGGAAATCTCCGCT
CAAAATACCGAGTATGTCGGCTATCTGACCCCCAATAAAGATGCGCTGCCACTCATTTCCGCCGAGGTGCGTGAATTGTA
CGCACAAGGCTTTGCCCCCGATGACGCGATGTATCAGCGCCTTGAGTGGGCAGTGCGTAATGAGGGCACGACTGCCTTCG
ATGATCTGTGGACTGAGATCAAGGGTGAGTAG

Upstream 100 bases:

>100_bases
CTACCCTGATGCTGCTGTTGTCGATGGTGCTGGTCGCACTGTCGCAACTTTTGCAGCGACGACGATAGGTCTGGATACAC
AGTAGTGAGGGAGGTTGAGA

Downstream 100 bases:

>100_bases
GCAGTACGAACATGTCTGATTGCAGGAGAGGAGTATGCACTGGCATGCTCCTCTCTGATTCTTCAGGGCTTTGTCTGTGC
TTCAGGCCCCTGGTTCACAT

Product: family 1 extracellular solute-binding protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: SPBP [H]

Number of amino acids: Translated: 383; Mature: 383

Protein sequence:

>383_residues
MQMLRLLVLVLLLILTACGGGGATTSPGNEYGTGSGDTTQTTGDGVDRSRLSDTLYFYNWSDYIDPAILDQFKAEYGVEV
VVDTYDSNEDMLAKVRAGNSGYDIVVPSDYAVQIMVAEGLAAPIDKSLLTNLVHIDPNLLDQYFDKGNAYSVPYMYGITG
IAYNTKFFPNGIDSWAAIFEPDQIAQFAGKFSMLDDARETPGAALRYIGQSLNSTDPAALERVKEILLTQKQYLAAYNSS
DVNRKLASEEYVMAHAWSGTAMQARNGLGDEFSGNPNIAFVIPKEGGMIWMDNLVILKDSPNAYTAHVFINFLMRPEISA
QNTEYVGYLTPNKDALPLISAEVRELYAQGFAPDDAMYQRLEWAVRNEGTTAFDDLWTEIKGE

Sequences:

>Translated_383_residues
MQMLRLLVLVLLLILTACGGGGATTSPGNEYGTGSGDTTQTTGDGVDRSRLSDTLYFYNWSDYIDPAILDQFKAEYGVEV
VVDTYDSNEDMLAKVRAGNSGYDIVVPSDYAVQIMVAEGLAAPIDKSLLTNLVHIDPNLLDQYFDKGNAYSVPYMYGITG
IAYNTKFFPNGIDSWAAIFEPDQIAQFAGKFSMLDDARETPGAALRYIGQSLNSTDPAALERVKEILLTQKQYLAAYNSS
DVNRKLASEEYVMAHAWSGTAMQARNGLGDEFSGNPNIAFVIPKEGGMIWMDNLVILKDSPNAYTAHVFINFLMRPEISA
QNTEYVGYLTPNKDALPLISAEVRELYAQGFAPDDAMYQRLEWAVRNEGTTAFDDLWTEIKGE
>Mature_383_residues
MQMLRLLVLVLLLILTACGGGGATTSPGNEYGTGSGDTTQTTGDGVDRSRLSDTLYFYNWSDYIDPAILDQFKAEYGVEV
VVDTYDSNEDMLAKVRAGNSGYDIVVPSDYAVQIMVAEGLAAPIDKSLLTNLVHIDPNLLDQYFDKGNAYSVPYMYGITG
IAYNTKFFPNGIDSWAAIFEPDQIAQFAGKFSMLDDARETPGAALRYIGQSLNSTDPAALERVKEILLTQKQYLAAYNSS
DVNRKLASEEYVMAHAWSGTAMQARNGLGDEFSGNPNIAFVIPKEGGMIWMDNLVILKDSPNAYTAHVFINFLMRPEISA
QNTEYVGYLTPNKDALPLISAEVRELYAQGFAPDDAMYQRLEWAVRNEGTTAFDDLWTEIKGE

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787367, Length=317, Percent_Identity=40.3785488958991, Blast_Score=218, Evalue=7e-58,
Organism=Escherichia coli, GI1787078, Length=307, Percent_Identity=35.8306188925081, Blast_Score=182, Evalue=3e-47,
Organism=Escherichia coli, GI1787711, Length=292, Percent_Identity=27.0547945205479, Blast_Score=84, Evalue=2e-17,

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 42202; Mature: 42202

Theoretical pI: Translated: 4.11; Mature: 4.11

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQMLRLLVLVLLLILTACGGGGATTSPGNEYGTGSGDTTQTTGDGVDRSRLSDTLYFYNW
CHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCEEEEECC
SDYIDPAILDQFKAEYGVEVVVDTYDSNEDMLAKVRAGNSGYDIVVPSDYAVQIMVAEGL
HHHCCHHHHHHHHHHCCCEEEEEECCCCCCCEEEEECCCCCCEEEECCCCEEEEEEECCC
AAPIDKSLLTNLVHIDPNLLDQYFDKGNAYSVPYMYGITGIAYNTKFFPNGIDSWAAIFE
CCCCCHHHHHHHHHCCHHHHHHHHCCCCCEECCHHHCCEEEEEECCCCCCCCCCHHHHCC
PDQIAQFAGKFSMLDDARETPGAALRYIGQSLNSTDPAALERVKEILLTQKQYLAAYNSS
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
DVNRKLASEEYVMAHAWSGTAMQARNGLGDEFSGNPNIAFVIPKEGGMIWMDNLVILKDS
HHHHHHCCCCEEEEEECCCCHHHHHCCCCCCCCCCCCEEEEEECCCCEEEECCEEEEECC
PNAYTAHVFINFLMRPEISAQNTEYVGYLTPNKDALPLISAEVRELYAQGFAPDDAMYQR
CCCEEHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCHHHHHH
LEWAVRNEGTTAFDDLWTEIKGE
HHHHHHCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MQMLRLLVLVLLLILTACGGGGATTSPGNEYGTGSGDTTQTTGDGVDRSRLSDTLYFYNW
CHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCEEEEECC
SDYIDPAILDQFKAEYGVEVVVDTYDSNEDMLAKVRAGNSGYDIVVPSDYAVQIMVAEGL
HHHCCHHHHHHHHHHCCCEEEEEECCCCCCCEEEEECCCCCCEEEECCCCEEEEEEECCC
AAPIDKSLLTNLVHIDPNLLDQYFDKGNAYSVPYMYGITGIAYNTKFFPNGIDSWAAIFE
CCCCCHHHHHHHHHCCHHHHHHHHCCCCCEECCHHHCCEEEEEECCCCCCCCCCHHHHCC
PDQIAQFAGKFSMLDDARETPGAALRYIGQSLNSTDPAALERVKEILLTQKQYLAAYNSS
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
DVNRKLASEEYVMAHAWSGTAMQARNGLGDEFSGNPNIAFVIPKEGGMIWMDNLVILKDS
HHHHHHCCCCEEEEEECCCCHHHHHCCCCCCCCCCCCEEEEEECCCCEEEECCEEEEECC
PNAYTAHVFINFLMRPEISAQNTEYVGYLTPNKDALPLISAEVRELYAQGFAPDDAMYQR
CCCEEHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCHHHHHH
LEWAVRNEGTTAFDDLWTEIKGE
HHHHHHCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1939142; 8905232; 9278503; 9298646; 8194593; 8621624; 8897598 [H]