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The map label for this gene is potB [H]

Identifier: 222525086

GI number: 222525086

Start: 2306106

End: 2306975

Strand: Direct

Name: potB [H]

Synonym: Chy400_1823

Alternate gene names: 222525086

Gene position: 2306106-2306975 (Clockwise)

Preceding gene: 222525085

Following gene: 222525087

Centisome position: 43.77

GC content: 53.1

Gene sequence:

>870_bases
ATGACCATGCTTCAACAGCAACAACAGCGTCGCGACCGTGTCCGGCTCTTGTTGCTGCTCTCTCCTGGTCTGCTCTGGCT
CTTACTCTTCTTTGCGTTGCCGTTGGTCATTATTCTTGTCTACAGCTTTATGACCAACGATGCATTGGGACGGGTTGTCT
ATCAACCAACGCTCGATAACTACATTACGATCTTCACGCAGAGCCTGTACGTTAACGCCTACTGGCGCTCGATCTGGACC
AGTGTCCTGACGACGGTGATTTGCCTGTTGATCGGCTATCCGCTGGCGCTCTTCATTGCCCGCAGTCCGCAACAATGGCG
TATGCCGCTGCTGATGCTGATTCTGATTCCATTTTGGACCAACTTTCTGGTGCGTATCTATGCCTGGCAGATCATTCTGG
CCAACAATGGGATTATCAACGGTTTCCTTGAAATCATCGGGGTCGGTCGGCTCCCTTTGCTCAACAACGAAGGCGCTACA
CTCCTTGGCCTGGTCTACGGTGAATTACCGTTTATGGTGTTGCCACTCTATGCCGCACTTGATCGTTTCGATTTCACGCT
GATGGAAGCCGCTGCCGATCTTGGCGCAAACAAGGTACAGGCTTTTCTGCGCGTGATGCTGCCAATGACGATGCCAGGGA
TCGTGGCCGGATCGGTGCTGGTCTTCATTCCAACCCTCGGTCAGTTCGTTGTCTCTGAACTCCTCGGCGGGGCAAAGGTT
GACTACCTGGGTAATCTGATTCAGCGGCTCTTCCTGCGGGCAAATCCGATTAACTGGCCACTCGGCTCAGCAATGGCTGT
GCTGATGATGATCGTGCTGCTGGTGCTGATTATGATGTACTTCCGCGCAACAACGGAGGAAGAGCGATGA

Upstream 100 bases:

>100_bases
CTCGACGCTTGACCGTGATGAATACTGGCAGCCGGGCGAAGCCGCCATCATTGTCTGTCCACCCGACAATGCATTGGTGC
TCAGTGAATAGGAGGGCGCG

Downstream 100 bases:

>100_bases
TCAAGACAGCACCCGCTTCAACCACGCTGACCCGCGCCCGTGGTGCGTTTGCTGAGGGGCGCCTGACGTGGAGCGGTGTG
GGATTGACGATCTTCTCAAT

Product: binding-protein-dependent transport systems inner membrane component

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 289; Mature: 288

Protein sequence:

>289_residues
MTMLQQQQQRRDRVRLLLLLSPGLLWLLLFFALPLVIILVYSFMTNDALGRVVYQPTLDNYITIFTQSLYVNAYWRSIWT
SVLTTVICLLIGYPLALFIARSPQQWRMPLLMLILIPFWTNFLVRIYAWQIILANNGIINGFLEIIGVGRLPLLNNEGAT
LLGLVYGELPFMVLPLYAALDRFDFTLMEAAADLGANKVQAFLRVMLPMTMPGIVAGSVLVFIPTLGQFVVSELLGGAKV
DYLGNLIQRLFLRANPINWPLGSAMAVLMMIVLLVLIMMYFRATTEEER

Sequences:

>Translated_289_residues
MTMLQQQQQRRDRVRLLLLLSPGLLWLLLFFALPLVIILVYSFMTNDALGRVVYQPTLDNYITIFTQSLYVNAYWRSIWT
SVLTTVICLLIGYPLALFIARSPQQWRMPLLMLILIPFWTNFLVRIYAWQIILANNGIINGFLEIIGVGRLPLLNNEGAT
LLGLVYGELPFMVLPLYAALDRFDFTLMEAAADLGANKVQAFLRVMLPMTMPGIVAGSVLVFIPTLGQFVVSELLGGAKV
DYLGNLIQRLFLRANPINWPLGSAMAVLMMIVLLVLIMMYFRATTEEER
>Mature_288_residues
TMLQQQQQRRDRVRLLLLLSPGLLWLLLFFALPLVIILVYSFMTNDALGRVVYQPTLDNYITIFTQSLYVNAYWRSIWTS
VLTTVICLLIGYPLALFIARSPQQWRMPLLMLILIPFWTNFLVRIYAWQIILANNGIINGFLEIIGVGRLPLLNNEGATL
LGLVYGELPFMVLPLYAALDRFDFTLMEAAADLGANKVQAFLRVMLPMTMPGIVAGSVLVFIPTLGQFVVSELLGGAKVD
YLGNLIQRLFLRANPINWPLGSAMAVLMMIVLLVLIMMYFRATTEEER

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787369, Length=261, Percent_Identity=43.6781609195402, Blast_Score=207, Evalue=9e-55,
Organism=Escherichia coli, GI1787080, Length=249, Percent_Identity=42.570281124498, Blast_Score=196, Evalue=2e-51,
Organism=Escherichia coli, GI1787713, Length=257, Percent_Identity=30.7392996108949, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI1789861, Length=292, Percent_Identity=27.7397260273973, Blast_Score=77, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 32782; Mature: 32651

Theoretical pI: Translated: 9.22; Mature: 9.22

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TMLQQQQQRRDRVRLLLLLSPGLLWLLLFFALPLVIILVYSFMTNDALGRVVYQPTLDN
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCHHH
YITIFTQSLYVNAYWRSIWTSVLTTVICLLIGYPLALFIARSPQQWRMPLLMLILIPFWT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
NFLVRIYAWQIILANNGIINGFLEIIGVGRLPLLNNEGATLLGLVYGELPFMVLPLYAAL
HHHHHHHHHHHHEECCCHHHHHHHHHHCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHH
DRFDFTLMEAAADLGANKVQAFLRVMLPMTMPGIVAGSVLVFIPTLGQFVVSELLGGAKV
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHH
DYLGNLIQRLFLRANPINWPLGSAMAVLMMIVLLVLIMMYFRATTEEER
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]