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The map label for this gene is ldh [H]

Identifier: 222525079

GI number: 222525079

Start: 2295539

End: 2296489

Strand: Direct

Name: ldh [H]

Synonym: Chy400_1816

Alternate gene names: 222525079

Gene position: 2295539-2296489 (Clockwise)

Preceding gene: 222525078

Following gene: 222525080

Centisome position: 43.57

GC content: 52.58

Gene sequence:

>951_bases
ATGAGTTTGCGTAAACGCACCGGTAAAGTTGGCGTCGTCGGAACAGGCATGGTTGGTACCTCCTTTGCCTATGCACTGAT
GCAGCGCAGCCTGGCCAGCGAACTGGTGTTGATTGATATTGATCATGCCCGTGCAGAAGGGGAGGCGATGGATCTTAATC
ACGGCCTGCCCTTTGTTCGCCCAATGCGCATCTATGCCGGTACCTATGATGATCTGGCCGGCGCCGACTTGATAGTCATC
GCTGCCGGGGCAAATCAGCGGCCTGGCGAGACCCGGCTCGATTTATTGAATCGGAATGTAGCCATTTTCCGTGAGATCAT
TCCTCCAATTCTGGCAGCCAATGATGATGGAATCATCGTTGTTGCCACTAATCCAGTGGACATCTTAACCACCATTGGGG
CACAGATTGCCGGTCCCGCAGCGAATCGAGTGATCGGATCAGGGACCATCCTTGATACGGCACGCTTTCGCTACTTGCTT
GGTCAGCACTACGGTGTCGATCCCCGCTCTGTGCACGCCTACATTGTCGGCGAACATGGCGATAGCGAGTTAGCGCTCTG
GAGCCTGGCAAATATTGCCGGAGTCAGGCTAGTTGATTTTGTCGGTGCAAATGGTCAGGGCTACAATCAGGCAGCGCTTG
ATGCGATTCTCGAACAAACCCGTAACGCTGCATACGAGATTATTAAGCGCAAGCGGGCAACCTATTATGCTATCGGTCTG
GGACTGCTATCGATTGCCGAAGCAGTGCTGCGTGATCAACACACGGTGATGACGATCAGCAGCTTGATGAACGGTCAGTA
TGGAGTAAGCGGGATTGCAATCAGTCTACCAACAATCGTTGGTCGTGATGGTGCTGAGGAGGTGCTCAATTTACCATTGT
CCGAACAGGAGATTGCTCTCTTTCAGCGCTCTGCCGGTATTCTAAAAGAACACCTGTCTCACGTTCAGTAA

Upstream 100 bases:

>100_bases
GAATTCTGGCGTTGTGCGGCGCTATATCCCTGTAATCTGAACCGGATACGCAATCTCTGGTGCGATATGGTATGATAAGA
GCGAACAGAAGGAGTATGCT

Downstream 100 bases:

>100_bases
AGAGATCGCTGTATCGTGTACACTTGAGGAGAGTGCGCCGTGCCCCACACCATTATGGTTGTTGAAGATGATGCTGCTAC
TCGCCGGCTTTATCGTTTTC

Product: L-lactate dehydrogenase

Products: NA

Alternate protein names: L-LDH [H]

Number of amino acids: Translated: 316; Mature: 315

Protein sequence:

>316_residues
MSLRKRTGKVGVVGTGMVGTSFAYALMQRSLASELVLIDIDHARAEGEAMDLNHGLPFVRPMRIYAGTYDDLAGADLIVI
AAGANQRPGETRLDLLNRNVAIFREIIPPILAANDDGIIVVATNPVDILTTIGAQIAGPAANRVIGSGTILDTARFRYLL
GQHYGVDPRSVHAYIVGEHGDSELALWSLANIAGVRLVDFVGANGQGYNQAALDAILEQTRNAAYEIIKRKRATYYAIGL
GLLSIAEAVLRDQHTVMTISSLMNGQYGVSGIAISLPTIVGRDGAEEVLNLPLSEQEIALFQRSAGILKEHLSHVQ

Sequences:

>Translated_316_residues
MSLRKRTGKVGVVGTGMVGTSFAYALMQRSLASELVLIDIDHARAEGEAMDLNHGLPFVRPMRIYAGTYDDLAGADLIVI
AAGANQRPGETRLDLLNRNVAIFREIIPPILAANDDGIIVVATNPVDILTTIGAQIAGPAANRVIGSGTILDTARFRYLL
GQHYGVDPRSVHAYIVGEHGDSELALWSLANIAGVRLVDFVGANGQGYNQAALDAILEQTRNAAYEIIKRKRATYYAIGL
GLLSIAEAVLRDQHTVMTISSLMNGQYGVSGIAISLPTIVGRDGAEEVLNLPLSEQEIALFQRSAGILKEHLSHVQ
>Mature_315_residues
SLRKRTGKVGVVGTGMVGTSFAYALMQRSLASELVLIDIDHARAEGEAMDLNHGLPFVRPMRIYAGTYDDLAGADLIVIA
AGANQRPGETRLDLLNRNVAIFREIIPPILAANDDGIIVVATNPVDILTTIGAQIAGPAANRVIGSGTILDTARFRYLLG
QHYGVDPRSVHAYIVGEHGDSELALWSLANIAGVRLVDFVGANGQGYNQAALDAILEQTRNAAYEIIKRKRATYYAIGLG
LLSIAEAVLRDQHTVMTISSLMNGQYGVSGIAISLPTIVGRDGAEEVLNLPLSEQEIALFQRSAGILKEHLSHVQ

Specific function: Catalyzes The Reversible Oxidation Of Malate To Oxaloacetate. [C]

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. LDH family [H]

Homologues:

Organism=Homo sapiens, GI9257228, Length=302, Percent_Identity=42.3841059602649, Blast_Score=243, Evalue=1e-64,
Organism=Homo sapiens, GI4504973, Length=302, Percent_Identity=42.3841059602649, Blast_Score=243, Evalue=1e-64,
Organism=Homo sapiens, GI47059044, Length=304, Percent_Identity=40.1315789473684, Blast_Score=242, Evalue=3e-64,
Organism=Homo sapiens, GI221136809, Length=304, Percent_Identity=40.1315789473684, Blast_Score=242, Evalue=3e-64,
Organism=Homo sapiens, GI5031857, Length=308, Percent_Identity=39.9350649350649, Blast_Score=241, Evalue=9e-64,
Organism=Homo sapiens, GI260099723, Length=308, Percent_Identity=39.9350649350649, Blast_Score=240, Evalue=1e-63,
Organism=Homo sapiens, GI15082234, Length=304, Percent_Identity=39.1447368421053, Blast_Score=239, Evalue=3e-63,
Organism=Homo sapiens, GI291575128, Length=304, Percent_Identity=39.4736842105263, Blast_Score=236, Evalue=2e-62,
Organism=Homo sapiens, GI4557032, Length=304, Percent_Identity=39.4736842105263, Blast_Score=236, Evalue=2e-62,
Organism=Homo sapiens, GI260099727, Length=190, Percent_Identity=46.8421052631579, Blast_Score=186, Evalue=3e-47,
Organism=Homo sapiens, GI260099725, Length=190, Percent_Identity=46.8421052631579, Blast_Score=185, Evalue=5e-47,
Organism=Homo sapiens, GI207028494, Length=195, Percent_Identity=38.4615384615385, Blast_Score=147, Evalue=1e-35,
Organism=Homo sapiens, GI103472011, Length=299, Percent_Identity=25.752508361204, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI103472015, Length=187, Percent_Identity=28.3422459893048, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI21735621, Length=307, Percent_Identity=28.6644951140065, Blast_Score=83, Evalue=4e-16,
Organism=Escherichia coli, GI1789632, Length=294, Percent_Identity=27.2108843537415, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17535107, Length=291, Percent_Identity=41.5807560137457, Blast_Score=228, Evalue=3e-60,
Organism=Caenorhabditis elegans, GI17554310, Length=298, Percent_Identity=25.503355704698, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI17136226, Length=304, Percent_Identity=38.8157894736842, Blast_Score=235, Evalue=3e-62,
Organism=Drosophila melanogaster, GI45550422, Length=312, Percent_Identity=31.4102564102564, Blast_Score=165, Evalue=3e-41,
Organism=Drosophila melanogaster, GI24647881, Length=331, Percent_Identity=28.0966767371601, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24663599, Length=331, Percent_Identity=25.9818731117825, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001557
- InterPro:   IPR011304
- InterPro:   IPR018177
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N [H]

EC number: =1.1.1.27 [H]

Molecular weight: Translated: 33833; Mature: 33702

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: PS00064 L_LDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLRKRTGKVGVVGTGMVGTSFAYALMQRSLASELVLIDIDHARAEGEAMDLNHGLPFVR
CCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCEEECCCCCCCCE
PMRIYAGTYDDLAGADLIVIAAGANQRPGETRLDLLNRNVAIFREIIPPILAANDDGIIV
EHHEECCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHCCHHHHHHHHHHHHCCCCCCEEE
VATNPVDILTTIGAQIAGPAANRVIGSGTILDTARFRYLLGQHYGVDPRSVHAYIVGEHG
EECCCHHHHHHHHHHHCCCHHCCEECCCCEEHHHHHHHHHHCCCCCCCCCEEEEEEECCC
DSELALWSLANIAGVRLVDFVGANGQGYNQAALDAILEQTRNAAYEIIKRKRATYYAIGL
CCCEEHHHHHHHCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEHHHH
GLLSIAEAVLRDQHTVMTISSLMNGQYGVSGIAISLPTIVGRDGAEEVLNLPLSEQEIAL
HHHHHHHHHHCCCHHHEEHHHHHCCCCCCCEEEEECCHHHCCCCHHHHHCCCCCHHHHHH
FQRSAGILKEHLSHVQ
HHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SLRKRTGKVGVVGTGMVGTSFAYALMQRSLASELVLIDIDHARAEGEAMDLNHGLPFVR
CCCCCCCCEEEEECCHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCEEECCCCCCCCE
PMRIYAGTYDDLAGADLIVIAAGANQRPGETRLDLLNRNVAIFREIIPPILAANDDGIIV
EHHEECCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHCCHHHHHHHHHHHHCCCCCCEEE
VATNPVDILTTIGAQIAGPAANRVIGSGTILDTARFRYLLGQHYGVDPRSVHAYIVGEHG
EECCCHHHHHHHHHHHCCCHHCCEECCCCEEHHHHHHHHHHCCCCCCCCCEEEEEEECCC
DSELALWSLANIAGVRLVDFVGANGQGYNQAALDAILEQTRNAAYEIIKRKRATYYAIGL
CCCEEHHHHHHHCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEHHHH
GLLSIAEAVLRDQHTVMTISSLMNGQYGVSGIAISLPTIVGRDGAEEVLNLPLSEQEIAL
HHHHHHHHHHCCCHHHEEHHHHHCCCCCCCEEEEECCHHHCCCCHHHHHCCCCCHHHHHH
FQRSAGILKEHLSHVQ
HHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA