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The map label for this gene is lacA [C]

Identifier: 222525043

GI number: 222525043

Start: 2247230

End: 2247772

Strand: Direct

Name: lacA [C]

Synonym: Chy400_1779

Alternate gene names: 222525043

Gene position: 2247230-2247772 (Clockwise)

Preceding gene: 222525041

Following gene: 222525044

Centisome position: 42.65

GC content: 55.8

Gene sequence:

>543_bases
ATGCCATCACGTCCGCTAACCGACATTGTGAGCGATATTCTGGCTGTGTTGCGGGCACGCTGGTATCTCCGTAGTGCGAC
ATTGGGGCCACGAGTACGGTTGTGGGGGCGTCCGGCAATCAGTAATTATGGCCAGCTCATCATCGGTGAGCGAGCGCGAT
TGGTTTCAACGATTGTGCCACTCGAGCTGGCAGTAGCTCATGGGGCGCGGTTAGAGATTGGTCAGGGGACGTTTATCAAC
TATGGTTGTTCAATTGCGGCAACTGAACTGGTACGTATCGGGCCGCGTTGTAATATCGGTACCTACGCCATGATTATGGA
CAACGACTTCCACCGTCTGGAGCCAGAGCGGCGCCAGGAACGTCCACCATCAGCCCCAATTATTCTGGAAGAGAACGTCT
GGCTCGGTGGGCGGGTAACGGTATTGAGCGGTGTGACGATTGGCGCTGGTAGTGTGATCGGTGCCGGTAGTGTGGTGACC
AAGAGCATCCCGCCGCGTTCATTGGCAGCAGGAGTCCCGGCACGTGTGATTAGACAGTTATAA

Upstream 100 bases:

>100_bases
GAACTCAGCAACGAAACTGTCAGCTTGCAAGAGCAGTTTGTTTAGAATCCGTTCATCTCAGTTGGGTAATGTAGACATGG
CAGAATAGGGAGGAACAGCT

Downstream 100 bases:

>100_bases
GCGTATGAATCAGAATCCTTCACTCAGTGTCAGCATTGTTATTCCAACCTATAATCGGGTAGAGCGCTTGCAGCGGGTGC
TGGCGGCTGTTAGCGGCCAG

Product: transferase hexapeptide repeat containing protein

Products: CoA; aacetyl-beta-D-galactoside [C]

Alternate protein names: NA

Number of amino acids: Translated: 180; Mature: 179

Protein sequence:

>180_residues
MPSRPLTDIVSDILAVLRARWYLRSATLGPRVRLWGRPAISNYGQLIIGERARLVSTIVPLELAVAHGARLEIGQGTFIN
YGCSIAATELVRIGPRCNIGTYAMIMDNDFHRLEPERRQERPPSAPIILEENVWLGGRVTVLSGVTIGAGSVIGAGSVVT
KSIPPRSLAAGVPARVIRQL

Sequences:

>Translated_180_residues
MPSRPLTDIVSDILAVLRARWYLRSATLGPRVRLWGRPAISNYGQLIIGERARLVSTIVPLELAVAHGARLEIGQGTFIN
YGCSIAATELVRIGPRCNIGTYAMIMDNDFHRLEPERRQERPPSAPIILEENVWLGGRVTVLSGVTIGAGSVIGAGSVVT
KSIPPRSLAAGVPARVIRQL
>Mature_179_residues
PSRPLTDIVSDILAVLRARWYLRSATLGPRVRLWGRPAISNYGQLIIGERARLVSTIVPLELAVAHGARLEIGQGTFINY
GCSIAATELVRIGPRCNIGTYAMIMDNDFHRLEPERRQERPPSAPIILEENVWLGGRVTVLSGVTIGAGSVIGAGSVVTK
SIPPRSLAAGVPARVIRQL

Specific function: May Assist Cellular Detoxification By Acetylating Nonmetabolizable Pyranosides, Thereby Preventing Their Reentry Into The Cell. [C]

COG id: COG0110

COG function: function code R; Acetyltransferase (isoleucine patch superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family [H]

Homologues:

Organism=Escherichia coli, GI1786537, Length=121, Percent_Identity=39.6694214876033, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1786664, Length=115, Percent_Identity=33.0434782608696, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1788345, Length=149, Percent_Identity=33.5570469798658, Blast_Score=67, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6322243, Length=119, Percent_Identity=36.9747899159664, Blast_Score=72, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001451
- InterPro:   IPR011004 [H]

Pfam domain/function: PF00132 Hexapep [H]

EC number: 2.3.1.18 [C]

Molecular weight: Translated: 19468; Mature: 19337

Theoretical pI: Translated: 11.08; Mature: 11.08

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSRPLTDIVSDILAVLRARWYLRSATLGPRVRLWGRPAISNYGQLIIGERARLVSTIVP
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCEEEECCHHHHHHHHHH
LELAVAHGARLEIGQGTFINYGCSIAATELVRIGPRCNIGTYAMIMDNDFHRLEPERRQE
HHHHHHCCCEEEECCCCEEECCCCHHHHHHHHCCCCCCCCEEEEEECCCCHHCCCHHHHC
RPPSAPIILEENVWLGGRVTVLSGVTIGAGSVIGAGSVVTKSIPPRSLAAGVPARVIRQL
CCCCCCEEEECCCEECCEEEEEECCEECCCCEECCCCEEECCCCCHHHHCCCHHHHHHCC
>Mature Secondary Structure 
PSRPLTDIVSDILAVLRARWYLRSATLGPRVRLWGRPAISNYGQLIIGERARLVSTIVP
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCEEEECCHHHHHHHHHH
LELAVAHGARLEIGQGTFINYGCSIAATELVRIGPRCNIGTYAMIMDNDFHRLEPERRQE
HHHHHHCCCEEEECCCCEEECCCCHHHHHHHHCCCCCCCCEEEEEECCCCHHCCCHHHHC
RPPSAPIILEENVWLGGRVTVLSGVTIGAGSVIGAGSVVTKSIPPRSLAAGVPARVIRQL
CCCCCCEEEECCCEECCEEEEEECCEECCCCEECCCCEEECCCCCHHHHCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: MgSO4; Mn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 1500 {isopropyl} 1200 {isopropyl} 770 {isopropyl} 360 {isopropyl} 0.18 {acetyl-CoA}} 0.049 {acetyl-CoA}} 0.045 {acetyl-CoA}} 0.04 {acetyl-CoA}} [C]

Substrates: Acetyl-CoA; a beta-D-galactoside [C]

Specific reaction: Acetyl-CoA + a beta-D-galactoside = CoA + aacetyl-beta-D-galactoside [C]

General reaction: Acyl group transfer [C]

Inhibitor: 1, 10-Phenanthroline; Acetyl-CoA; Co2+; CoA; Cu2+; MgSO4; Mn2+; p-Chloromercuribenzoate; Zn2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10708580 [H]