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The map label for this gene is hemK [H]

Identifier: 222525022

GI number: 222525022

Start: 2219009

End: 2219860

Strand: Direct

Name: hemK [H]

Synonym: Chy400_1757

Alternate gene names: 222525022

Gene position: 2219009-2219860 (Clockwise)

Preceding gene: 222525021

Following gene: 222525023

Centisome position: 42.11

GC content: 57.86

Gene sequence:

>852_bases
ATGACGATCCAACAAGCCATTCAGCACGCAATCACCCAATTGCGAACCATCTCGCCGACCGCCCGGCTTGACGCCGAACT
CCTGCTTGCTCACATTCTCGGCTGGTCGCGGGCAAAAGTAGTTGCTGAACGGGATCATGTCCTGACGCCAGAGCAAGAGA
TGGCCTTCAACGCCTTGATCGAACGTCGGGCCAACCGTGAGCCGGTTGCGTACCTCATCGGCCATCGCGAGTTCTTTGGC
CTTGATCTGTTCGTTGACCGTCGCGTGCTCATCCCGCGCCCGGAAACCGAGTTGCTGGTCGAACTGACTCTCAAGGAAGC
GCAACGCTTCAACCACACCCCACTGATCATTGCCGACATCGGCACCGGTAGCGGTGCTATTGCAATTGCACTGGCTATGC
ACCTGCCGCACGCCCTGATCTACGGCGTTGACATCAGTCCTGATGCGCTGGCAGTAGCCGCCATCAACGTTACCCGCTAT
CGACTCGACGACCGGATCAGGCTGCTTGAAGGTGATCTCTGCACGCCACTCCCCGCCCCGGTCGATATTCTGGTCAGCAA
CCCACCATACACGATCCTGACTGAAATTGACGAGGGCGTCTATCGTCACGAACCACACCTTGCCCTCGATGGCGGCAGCG
ATGGCCTCGACTGCTACCGACGCCTGATCGCTGCTGCTCCGACCTATCTCAAACCCAACGGGGCGATCTTGCTGGAAATC
GGTTCAACCCAGGCTGCATCTGTGGTACACTTACTACGTCAGGCGTTGCCGATGGCAGAAACCGGTATAGAACGTGATCT
GGCCGGTCACGACAGGATCGTTTGGGCACGTAATCGATATGTCATCCACTGA

Upstream 100 bases:

>100_bases
TACTGTCGTTGGTGCCTGGCATGCGTTCATGCGCCAGTACGATGGTTGGGTAGCACGACAGCACAGCTTTCTGAGGTAAG
TTCTAACCTACAACAACCTT

Downstream 100 bases:

>100_bases
TCTGGCCCCTGGTTCTATCTTGTCGATATACTATGGGTAGAGATCAGGAGGATTGCTATGACTGCACTCTTTGTCGCCCT
TGGCATCGCCATCATTACTC

Product: protein-(glutamine-N5) methyltransferase, release factor-specific

Products: NA

Alternate protein names: M.XfaHemK2P [H]

Number of amino acids: Translated: 283; Mature: 282

Protein sequence:

>283_residues
MTIQQAIQHAITQLRTISPTARLDAELLLAHILGWSRAKVVAERDHVLTPEQEMAFNALIERRANREPVAYLIGHREFFG
LDLFVDRRVLIPRPETELLVELTLKEAQRFNHTPLIIADIGTGSGAIAIALAMHLPHALIYGVDISPDALAVAAINVTRY
RLDDRIRLLEGDLCTPLPAPVDILVSNPPYTILTEIDEGVYRHEPHLALDGGSDGLDCYRRLIAAAPTYLKPNGAILLEI
GSTQAASVVHLLRQALPMAETGIERDLAGHDRIVWARNRYVIH

Sequences:

>Translated_283_residues
MTIQQAIQHAITQLRTISPTARLDAELLLAHILGWSRAKVVAERDHVLTPEQEMAFNALIERRANREPVAYLIGHREFFG
LDLFVDRRVLIPRPETELLVELTLKEAQRFNHTPLIIADIGTGSGAIAIALAMHLPHALIYGVDISPDALAVAAINVTRY
RLDDRIRLLEGDLCTPLPAPVDILVSNPPYTILTEIDEGVYRHEPHLALDGGSDGLDCYRRLIAAAPTYLKPNGAILLEI
GSTQAASVVHLLRQALPMAETGIERDLAGHDRIVWARNRYVIH
>Mature_282_residues
TIQQAIQHAITQLRTISPTARLDAELLLAHILGWSRAKVVAERDHVLTPEQEMAFNALIERRANREPVAYLIGHREFFGL
DLFVDRRVLIPRPETELLVELTLKEAQRFNHTPLIIADIGTGSGAIAIALAMHLPHALIYGVDISPDALAVAAINVTRYR
LDDRIRLLEGDLCTPLPAPVDILVSNPPYTILTEIDEGVYRHEPHLALDGGSDGLDCYRRLIAAAPTYLKPNGAILLEIG
STQAASVVHLLRQALPMAETGIERDLAGHDRIVWARNRYVIH

Specific function: Probable protein methyltransferase. May methylate a Gln residue in target proteins [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hemK family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=278, Percent_Identity=35.6115107913669, Blast_Score=127, Evalue=1e-29,
Organism=Escherichia coli, GI1787463, Length=283, Percent_Identity=37.1024734982332, Blast_Score=142, Evalue=2e-35,
Organism=Escherichia coli, GI87082085, Length=246, Percent_Identity=36.9918699186992, Blast_Score=132, Evalue=3e-32,
Organism=Saccharomyces cerevisiae, GI6324266, Length=249, Percent_Identity=26.9076305220884, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24582226, Length=271, Percent_Identity=28.0442804428044, Blast_Score=102, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 31382; Mature: 31251

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIQQAIQHAITQLRTISPTARLDAELLLAHILGWSRAKVVAERDHVLTPEQEMAFNALI
CCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHEEECCCCCCCCHHHHHHHHHH
ERRANREPVAYLIGHREFFGLDLFVDRRVLIPRPETELLVELTLKEAQRFNHTPLIIADI
HHHCCCCCEEEEECCHHHCCEEEEECCEEECCCCCHHHHEEEEHHHHHHCCCCCEEEEEC
GTGSGAIAIALAMHLPHALIYGVDISPDALAVAAINVTRYRLDDRIRLLEGDLCTPLPAP
CCCCCHHHHHHHHHCCCEEEEECCCCCCCEEEEEEEEEEEECCCCEEEECCCCCCCCCCC
VDILVSNPPYTILTEIDEGVYRHEPHLALDGGSDGLDCYRRLIAAAPTYLKPNGAILLEI
EEEEECCCCEEEEEECCCCHHHCCCCEEECCCCCCHHHHHHHHHCCCCEECCCCEEEEEE
GSTQAASVVHLLRQALPMAETGIERDLAGHDRIVWARNRYVIH
CCCHHHHHHHHHHHHCCHHHHCCCCCCCCCCEEEEECCCEEEC
>Mature Secondary Structure 
TIQQAIQHAITQLRTISPTARLDAELLLAHILGWSRAKVVAERDHVLTPEQEMAFNALI
CHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHEEECCCCCCCCHHHHHHHHHH
ERRANREPVAYLIGHREFFGLDLFVDRRVLIPRPETELLVELTLKEAQRFNHTPLIIADI
HHHCCCCCEEEEECCHHHCCEEEEECCEEECCCCCHHHHEEEEHHHHHHCCCCCEEEEEC
GTGSGAIAIALAMHLPHALIYGVDISPDALAVAAINVTRYRLDDRIRLLEGDLCTPLPAP
CCCCCHHHHHHHHHCCCEEEEECCCCCCCEEEEEEEEEEEECCCCEEEECCCCCCCCCCC
VDILVSNPPYTILTEIDEGVYRHEPHLALDGGSDGLDCYRRLIAAAPTYLKPNGAILLEI
EEEEECCCCEEEEEECCCCHHHCCCCEEECCCCCCHHHHHHHHHCCCCEECCCCEEEEEE
GSTQAASVVHLLRQALPMAETGIERDLAGHDRIVWARNRYVIH
CCCHHHHHHHHHHHHCCHHHHCCCCCCCCCCEEEEECCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]