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The map label for this gene is rffG [C]

Identifier: 222525009

GI number: 222525009

Start: 2201426

End: 2202466

Strand: Direct

Name: rffG [C]

Synonym: Chy400_1744

Alternate gene names: 222525009

Gene position: 2201426-2202466 (Clockwise)

Preceding gene: 222525008

Following gene: 222525010

Centisome position: 41.78

GC content: 56.48

Gene sequence:

>1041_bases
ATGGCTGATCGTGTCTTAATTACTGGCGGAGCCGGTTTTCTGGGCATTAACCTGACGCGCTACCTGTTAGCGCGAGGGTA
TCTCGTGCGCTCACTTGATATTGCCCCCTTTGATTACCCCGAACGCAACCAGATTGAAGAGCACACGGGTGACATTCGTG
ACCGGGCTGCCGTTGATCGGGCGATGAAGGATGTGCGCTTCGTCGTTCACACCGCAGCAGCTCTGCCGTTGTACTCGCCG
GCAGACATCTTCTCAACCGATATTGATGGTACGCGCAACGTCCTCGAATCGGCTCGCGATCACGGGGTTGAGCGCGTTGT
CCACATCTCCTCAACCGCCGTGTATGGTATTCCTGACCATCACCCGCTGCTGGAAACCGATCCCCTGAGCGGCGTTGGCC
CCTACGGCGAGGCGAAAGTGAAAGCCGAAGAACTGTGCCTCGAATTCCGGCGTGCCGGTATGTGCGTGCCAATTCTCCGC
CCCAAGTCGTTCGTCGGCCCAGAGCGGCTGGGCATCTTCGCAATGCTGTACGACTGGGCAATGGATGGGCGCAACTTCCC
ACTACCGGGGAACGGCAAGAATCGCTACCAGCTCCTCGATGTTGAAGACCTTTGTGAAGCGATTGTACGCTGTCTGGAAC
TTGACCGTGATCGGGTCAACGACACCTTCAACATCGGTGCCAAAGAGTTCGGCACGATCAAAGAAGATTTTCAAGCCGTA
CTCGATGCCGCCGGTCATGGCAAGAAGATCATCACCTTCCCGGCAGCACCGATGGTGTGGGCACTGGCGATTTTAGAGAA
ACTGAAGCTGTCGCCGGTGTATAAATGGGCGTATGGCACAGTCACCGAAGACTCCTTCGTATCGGTGGAAAAAGCCGAGC
GCGTCCTGGGCTTCACGCCGAAGTATTCCAACAAAGAGGCGCTGGTGCGCAACTTCCAGTGGTACGTCGAGCACGCTAAC
CAGTTTGGTCAGCAAACTGGCGTCTCGCACCGGGTGCCCTGGAGTCAGGGTATTTTGCGGTTTGCGAAGCTGTTTTTCTA
A

Upstream 100 bases:

>100_bases
ACCGGTCGTTGATCACGATGGTCGACTGTCGGGTATAATCGCACGTGGTGCCCTGATCAGGGCCTTATTACAACAAAGCG
AATAGTAGGACTGGTAACCT

Downstream 100 bases:

>100_bases
TTCGCCTACAGCACATAGCTGACATCCTCAACAGCCGGAAAACAATGGTCTGGTCGGGGTGACGTAAGCGTCGCCTCGAC
CGATAATCACCAGGACGCGC

Product: NAD-dependent epimerase/dehydratase

Products: dTDP-4-dehydro-6-deoxy-D-glucose; H2O [C]

Alternate protein names: NA

Number of amino acids: Translated: 346; Mature: 345

Protein sequence:

>346_residues
MADRVLITGGAGFLGINLTRYLLARGYLVRSLDIAPFDYPERNQIEEHTGDIRDRAAVDRAMKDVRFVVHTAAALPLYSP
ADIFSTDIDGTRNVLESARDHGVERVVHISSTAVYGIPDHHPLLETDPLSGVGPYGEAKVKAEELCLEFRRAGMCVPILR
PKSFVGPERLGIFAMLYDWAMDGRNFPLPGNGKNRYQLLDVEDLCEAIVRCLELDRDRVNDTFNIGAKEFGTIKEDFQAV
LDAAGHGKKIITFPAAPMVWALAILEKLKLSPVYKWAYGTVTEDSFVSVEKAERVLGFTPKYSNKEALVRNFQWYVEHAN
QFGQQTGVSHRVPWSQGILRFAKLFF

Sequences:

>Translated_346_residues
MADRVLITGGAGFLGINLTRYLLARGYLVRSLDIAPFDYPERNQIEEHTGDIRDRAAVDRAMKDVRFVVHTAAALPLYSP
ADIFSTDIDGTRNVLESARDHGVERVVHISSTAVYGIPDHHPLLETDPLSGVGPYGEAKVKAEELCLEFRRAGMCVPILR
PKSFVGPERLGIFAMLYDWAMDGRNFPLPGNGKNRYQLLDVEDLCEAIVRCLELDRDRVNDTFNIGAKEFGTIKEDFQAV
LDAAGHGKKIITFPAAPMVWALAILEKLKLSPVYKWAYGTVTEDSFVSVEKAERVLGFTPKYSNKEALVRNFQWYVEHAN
QFGQQTGVSHRVPWSQGILRFAKLFF
>Mature_345_residues
ADRVLITGGAGFLGINLTRYLLARGYLVRSLDIAPFDYPERNQIEEHTGDIRDRAAVDRAMKDVRFVVHTAAALPLYSPA
DIFSTDIDGTRNVLESARDHGVERVVHISSTAVYGIPDHHPLLETDPLSGVGPYGEAKVKAEELCLEFRRAGMCVPILRP
KSFVGPERLGIFAMLYDWAMDGRNFPLPGNGKNRYQLLDVEDLCEAIVRCLELDRDRVNDTFNIGAKEFGTIKEDFQAVL
DAAGHGKKIITFPAAPMVWALAILEKLKLSPVYKWAYGTVTEDSFVSVEKAERVLGFTPKYSNKEALVRNFQWYVEHANQ
FGQQTGVSHRVPWSQGILRFAKLFF

Specific function: Putative nucleotide sugar epimerase/dehydrogenase [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI193211614, Length=342, Percent_Identity=25.7309941520468, Blast_Score=86, Evalue=7e-17,
Organism=Homo sapiens, GI8393516, Length=342, Percent_Identity=25.7309941520468, Blast_Score=86, Evalue=7e-17,
Organism=Homo sapiens, GI56237023, Length=174, Percent_Identity=33.9080459770115, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI56118217, Length=174, Percent_Identity=33.9080459770115, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI189083684, Length=174, Percent_Identity=33.9080459770115, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI42516563, Length=266, Percent_Identity=28.1954887218045, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI48994969, Length=367, Percent_Identity=26.9754768392371, Blast_Score=82, Evalue=7e-17,
Organism=Saccharomyces cerevisiae, GI6321437, Length=350, Percent_Identity=25.7142857142857, Blast_Score=67, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 4.2.1.46 [C]

Molecular weight: Translated: 38806; Mature: 38675

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADRVLITGGAGFLGINLTRYLLARGYLVRSLDIAPFDYPERNQIEEHTGDIRDRAAVDR
CCCCEEEECCCCCHHHHHHHHHHHHCHHHEEECCCCCCCCCCCCHHHHHCCHHHHHHHHH
AMKDVRFVVHTAAALPLYSPADIFSTDIDGTRNVLESARDHGVERVVHISSTAVYGIPDH
HHHHHHHHHHHHHHCCCCCCHHHHHCCCCHHHHHHHHHHHCCHHHEEEEECEEEECCCCC
HPLLETDPLSGVGPYGEAKVKAEELCLEFRRAGMCVPILRPKSFVGPERLGIFAMLYDWA
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHH
MDGRNFPLPGNGKNRYQLLDVEDLCEAIVRCLELDRDRVNDTFNIGAKEFGTIKEDFQAV
CCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCHHHHHHHH
LDAAGHGKKIITFPAAPMVWALAILEKLKLSPVYKWAYGTVTEDSFVSVEKAERVLGFTP
HHHCCCCCEEEEECCHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHCCCC
KYSNKEALVRNFQWYVEHANQFGQQTGVSHRVPWSQGILRFAKLFF
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHC
>Mature Secondary Structure 
ADRVLITGGAGFLGINLTRYLLARGYLVRSLDIAPFDYPERNQIEEHTGDIRDRAAVDR
CCCEEEECCCCCHHHHHHHHHHHHCHHHEEECCCCCCCCCCCCHHHHHCCHHHHHHHHH
AMKDVRFVVHTAAALPLYSPADIFSTDIDGTRNVLESARDHGVERVVHISSTAVYGIPDH
HHHHHHHHHHHHHHCCCCCCHHHHHCCCCHHHHHHHHHHHCCHHHEEEEECEEEECCCCC
HPLLETDPLSGVGPYGEAKVKAEELCLEFRRAGMCVPILRPKSFVGPERLGIFAMLYDWA
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHH
MDGRNFPLPGNGKNRYQLLDVEDLCEAIVRCLELDRDRVNDTFNIGAKEFGTIKEDFQAV
CCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCHHHHHHHH
LDAAGHGKKIITFPAAPMVWALAILEKLKLSPVYKWAYGTVTEDSFVSVEKAERVLGFTP
HHHCCCCCEEEEECCHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHCCCC
KYSNKEALVRNFQWYVEHANQFGQQTGVSHRVPWSQGILRFAKLFF
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.093 {dTDPglucose}} [C]

Substrates: dTDPglucose [C]

Specific reaction: dTDPglucose --> dTDP-4-dehydro-6-deoxy-D-glucose + H2O [C]

General reaction: Elimination (of H2O C-O bond cleavage [C]

Inhibitor: p-Chloromercuribenzoate; TMP [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]