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The map label for this gene is argF [H]

Identifier: 222524980

GI number: 222524980

Start: 2163588

End: 2164517

Strand: Reverse

Name: argF [H]

Synonym: Chy400_1715

Alternate gene names: 222524980

Gene position: 2164517-2163588 (Counterclockwise)

Preceding gene: 222524981

Following gene: 222524979

Centisome position: 41.08

GC content: 55.81

Gene sequence:

>930_bases
ATGACATTACGACATTTTCTCTCCGCAGCCGATCTTAACCGTGCTGAGGCGGAAGCCCTGCTTGATCGGGCCGCAATGCT
TAAAGCGGCATGGCGCGCCGGCCAGGTCAATGATCGGCCCTTGTTAGGCCAGACACTCGCTCTCGTCTTTGAAAAGCCTT
CTCTGCGCACACGGGTAGCCTTTGAAGCCGGTATGACCCAGCTCGGTGGACATCCTTCCTATCTCTCGGCAAATGACATT
GATATGGGCGGGCGTGAGAGTGTTCCTGACGTTGCTCGCAATTTAAGTCGCTGGGTGGCAATTATCGCGGCACGGGTGTT
CAAGCATGCCACGGTTGAGACTCTGGCGCGCTATGCGACGGTACCGGTTATTAATGCACTCTCGGATCGTGAGCATCCAT
GTCAGGCACTGGCCGATATGCTGACGCTCCGTGAACGCTTCGGACGCTTACAGGGTCTCACCCTGGCGTATGTTGGTGAT
GGAAATAATGTCTGTCATTCGTTGATGTTATTGGGTGCAACGCTGGGTCTCAACCTGCGGATTGGGTGTCCACCCGATTA
CCGACCGGCACCCGACATTATCGAACTAACGGAACGACTGGCCCAGGAACACGACGCCACGCTCGCCATTACAGCTTCTC
CGGTCGAAGCGGTTAGCGGAGCCGATGCAGTGTATACTGATGTCTGGGCATCGATGGGGCAGGAGCACGAAGCTGCACGT
CGTCGTCCGGTCTTTACACCGTATCAGGTAAACACAGCGCTTATGGCCCATGCGGCACCACACGCACTGGCGATGCACTG
CTTACCGGCGCATCGTGGTGAGGAAGTAACGGCTGAGGTTATCGACGGGCCGCAATCTGTCGTATTTGAGCAGGCAGAGA
ATCGACTCCACGTTCAAAAGGCGCTCATTTTGTTCTTGTTAGGGAAGTAG

Upstream 100 bases:

>100_bases
ACTCAGGAATGAACTGAGTGAAATACACGCTACGGCGCATACAATCCTGTCGGCGTTTGCCAGACCTGCCTGTGCCTGAA
TAGAAAGAAAGAATGTTACC

Downstream 100 bases:

>100_bases
CGCCGATAGCATGCGGAAAGACGCTATGGCAGGCAACCGGGCAATCTTTGATCGCGCAATGGAGCAGTGTCGGGACGCAT
CGGCACAAGGTCGTTGGGAA

Product: ornithine carbamoyltransferase

Products: NA

Alternate protein names: OTCase [H]

Number of amino acids: Translated: 309; Mature: 308

Protein sequence:

>309_residues
MTLRHFLSAADLNRAEAEALLDRAAMLKAAWRAGQVNDRPLLGQTLALVFEKPSLRTRVAFEAGMTQLGGHPSYLSANDI
DMGGRESVPDVARNLSRWVAIIAARVFKHATVETLARYATVPVINALSDREHPCQALADMLTLRERFGRLQGLTLAYVGD
GNNVCHSLMLLGATLGLNLRIGCPPDYRPAPDIIELTERLAQEHDATLAITASPVEAVSGADAVYTDVWASMGQEHEAAR
RRPVFTPYQVNTALMAHAAPHALAMHCLPAHRGEEVTAEVIDGPQSVVFEQAENRLHVQKALILFLLGK

Sequences:

>Translated_309_residues
MTLRHFLSAADLNRAEAEALLDRAAMLKAAWRAGQVNDRPLLGQTLALVFEKPSLRTRVAFEAGMTQLGGHPSYLSANDI
DMGGRESVPDVARNLSRWVAIIAARVFKHATVETLARYATVPVINALSDREHPCQALADMLTLRERFGRLQGLTLAYVGD
GNNVCHSLMLLGATLGLNLRIGCPPDYRPAPDIIELTERLAQEHDATLAITASPVEAVSGADAVYTDVWASMGQEHEAAR
RRPVFTPYQVNTALMAHAAPHALAMHCLPAHRGEEVTAEVIDGPQSVVFEQAENRLHVQKALILFLLGK
>Mature_308_residues
TLRHFLSAADLNRAEAEALLDRAAMLKAAWRAGQVNDRPLLGQTLALVFEKPSLRTRVAFEAGMTQLGGHPSYLSANDID
MGGRESVPDVARNLSRWVAIIAARVFKHATVETLARYATVPVINALSDREHPCQALADMLTLRERFGRLQGLTLAYVGDG
NNVCHSLMLLGATLGLNLRIGCPPDYRPAPDIIELTERLAQEHDATLAITASPVEAVSGADAVYTDVWASMGQEHEAARR
RPVFTPYQVNTALMAHAAPHALAMHCLPAHRGEEVTAEVIDGPQSVVFEQAENRLHVQKALILFLLGK

Specific function: Arginine biosynthesis; sixth step. [C]

COG id: COG0078

COG function: function code E; Ornithine carbamoyltransferase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI38788445, Length=306, Percent_Identity=41.8300653594771, Blast_Score=238, Evalue=4e-63,
Organism=Homo sapiens, GI18105007, Length=315, Percent_Identity=29.5238095238095, Blast_Score=112, Evalue=4e-25,
Organism=Escherichia coli, GI1790703, Length=329, Percent_Identity=38.6018237082067, Blast_Score=208, Evalue=4e-55,
Organism=Escherichia coli, GI1786469, Length=329, Percent_Identity=38.2978723404255, Blast_Score=202, Evalue=2e-53,
Organism=Escherichia coli, GI2367364, Length=319, Percent_Identity=28.8401253918495, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI48994908, Length=358, Percent_Identity=25.1396648044693, Blast_Score=75, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI193204318, Length=326, Percent_Identity=26.0736196319018, Blast_Score=86, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6322373, Length=323, Percent_Identity=35.6037151702786, Blast_Score=216, Evalue=4e-57,
Organism=Saccharomyces cerevisiae, GI6322331, Length=315, Percent_Identity=29.8412698412698, Blast_Score=104, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24642586, Length=312, Percent_Identity=27.2435897435897, Blast_Score=98, Evalue=9e-21,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2066 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002292 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.3 [H]

Molecular weight: Translated: 33659; Mature: 33528

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLRHFLSAADLNRAEAEALLDRAAMLKAAWRAGQVNDRPLLGQTLALVFEKPSLRTRVA
CCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCHHHHH
FEAGMTQLGGHPSYLSANDIDMGGRESVPDVARNLSRWVAIIAARVFKHATVETLARYAT
HHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VPVINALSDREHPCQALADMLTLRERFGRLQGLTLAYVGDGNNVCHSLMLLGATLGLNLR
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHCCEEE
IGCPPDYRPAPDIIELTERLAQEHDATLAITASPVEAVSGADAVYTDVWASMGQEHEAAR
ECCCCCCCCCCCHHHHHHHHHHHCCCEEEEECCCHHHHCCCHHHHHHHHHHCCCHHHHHH
RRPVFTPYQVNTALMAHAAPHALAMHCLPAHRGEEVTAEVIDGPQSVVFEQAENRLHVQK
CCCCCCCHHHHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHH
ALILFLLGK
HHHHHHCCC
>Mature Secondary Structure 
TLRHFLSAADLNRAEAEALLDRAAMLKAAWRAGQVNDRPLLGQTLALVFEKPSLRTRVA
CHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCHHHHH
FEAGMTQLGGHPSYLSANDIDMGGRESVPDVARNLSRWVAIIAARVFKHATVETLARYAT
HHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VPVINALSDREHPCQALADMLTLRERFGRLQGLTLAYVGDGNNVCHSLMLLGATLGLNLR
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHCCEEE
IGCPPDYRPAPDIIELTERLAQEHDATLAITASPVEAVSGADAVYTDVWASMGQEHEAAR
ECCCCCCCCCCCHHHHHHHHHHHCCCEEEEECCCHHHHCCCHHHHHHHHHHCCCHHHHHH
RRPVFTPYQVNTALMAHAAPHALAMHCLPAHRGEEVTAEVIDGPQSVVFEQAENRLHVQK
CCCCCCCHHHHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHH
ALILFLLGK
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA