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The map label for this gene is hpcH [H]

Identifier: 222524969

GI number: 222524969

Start: 2150353

End: 2151102

Strand: Reverse

Name: hpcH [H]

Synonym: Chy400_1704

Alternate gene names: 222524969

Gene position: 2151102-2150353 (Counterclockwise)

Preceding gene: 222524970

Following gene: 222524967

Centisome position: 40.83

GC content: 60.8

Gene sequence:

>750_bases
ATGGTTGATCACCTTCGCCAACGCTGGGCAAGCGACGCTGTGATCTACAATGCGTGGCTCACCATTCCCAGCCCTTGGAG
CGCTGAGCTACTGGCCGCAGCCGGATTTCCCTCGCTCACCATTGATCTGCAGCACGGCTTGATCGACGATCCAACCGCAT
TGCAAATCCTGCATACCATTGACCGACGGTGTCCGGTCTTTGTTCGCTTAGCGTGGAACGAACCGGCAGCGATTATGCGC
GCTCTGGATCGGGGTGCTGCCGGAATTATTGCGCCGTTGATTACCGGCCCAGCCGATGCCCAGGCGCTGGTTGCTGCCTG
TCGTTTTCCTCCAGAGGGCATCCGGAGTTATGGGCCGGTGCGGGTTGGCGTTGTGTATGGGGTGGCCGAACCGGCCATGG
TGGCGCGCCTGCCGTTCATTATGCCGATGATCGAAACGGCCGGCGCCCTCGACCAGCTTGAGGCAATTGCTGCTGTGCCG
GGCATTGATGGCCTCTATGTCGGGCCGGCTGATCTCAGCTTGAGTTTAGGGCTTCCCGTACCGGTGGATTTCGCAGCCCC
ACCCTTCCGAGCGGCGCTTAGCCGGGTGGTTGAGGTTTGTCGACGGCATCACCTGGTTGCCGGCATCTACGCCAGCCCGG
AATGGGCCGCCGATCTGGCGGCGCTTGGCTTTCGGTTCATCACGATTGTCAACGACAGTGATCTGATCAGTCGGGGGGCA
ATCGCGGCATTACAGCGTGTAAGCGGCTAA

Upstream 100 bases:

>100_bases
GTGATCAATGAGATAACTTCTGCTCCTACGCTGACCCTTTTCCCCACTCTGCAACCGTGCTACCATGCAGATGTTCGCTG
ATAGTATGGAGATACAAGCT

Downstream 100 bases:

>100_bases
TCTGAATCTAGGTCTCAGCACAGGCAGCCAGCGCGGCGCGGTCGCAAATCGCGATTGCGCCGCGTTCGATACGGATCATC
CCCTGCATCTCAAACTGGTG

Product: HpcH/HpaI aldolase

Products: NA

Alternate protein names: 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase; HHED aldolase; 4-hydroxy-2-ketoheptane-1,7-dioate aldolase; HKHD aldolase [H]

Number of amino acids: Translated: 249; Mature: 249

Protein sequence:

>249_residues
MVDHLRQRWASDAVIYNAWLTIPSPWSAELLAAAGFPSLTIDLQHGLIDDPTALQILHTIDRRCPVFVRLAWNEPAAIMR
ALDRGAAGIIAPLITGPADAQALVAACRFPPEGIRSYGPVRVGVVYGVAEPAMVARLPFIMPMIETAGALDQLEAIAAVP
GIDGLYVGPADLSLSLGLPVPVDFAAPPFRAALSRVVEVCRRHHLVAGIYASPEWAADLAALGFRFITIVNDSDLISRGA
IAALQRVSG

Sequences:

>Translated_249_residues
MVDHLRQRWASDAVIYNAWLTIPSPWSAELLAAAGFPSLTIDLQHGLIDDPTALQILHTIDRRCPVFVRLAWNEPAAIMR
ALDRGAAGIIAPLITGPADAQALVAACRFPPEGIRSYGPVRVGVVYGVAEPAMVARLPFIMPMIETAGALDQLEAIAAVP
GIDGLYVGPADLSLSLGLPVPVDFAAPPFRAALSRVVEVCRRHHLVAGIYASPEWAADLAALGFRFITIVNDSDLISRGA
IAALQRVSG
>Mature_249_residues
MVDHLRQRWASDAVIYNAWLTIPSPWSAELLAAAGFPSLTIDLQHGLIDDPTALQILHTIDRRCPVFVRLAWNEPAAIMR
ALDRGAAGIIAPLITGPADAQALVAACRFPPEGIRSYGPVRVGVVYGVAEPAMVARLPFIMPMIETAGALDQLEAIAAVP
GIDGLYVGPADLSLSLGLPVPVDFAAPPFRAALSRVVEVCRRHHLVAGIYASPEWAADLAALGFRFITIVNDSDLISRGA
IAALQRVSG

Specific function: Catalyzes the reversible retro-aldol cleavage of 4- hydroxy-2-ketoheptane-1,7-dioate (HKHD) to pyruvate and succinic semialdehyde [H]

COG id: COG3836

COG function: function code G; 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1789514, Length=232, Percent_Identity=29.3103448275862, Blast_Score=93, Evalue=1e-20,
Organism=Escherichia coli, GI1788578, Length=246, Percent_Identity=30.8943089430894, Blast_Score=88, Evalue=7e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR012689
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: 4.1.2.-

Molecular weight: Translated: 26480; Mature: 26480

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVDHLRQRWASDAVIYNAWLTIPSPWSAELLAAAGFPSLTIDLQHGLIDDPTALQILHTI
CCHHHHHHHCCCEEEEEEEEECCCCCCHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHH
DRRCPVFVRLAWNEPAAIMRALDRGAAGIIAPLITGPADAQALVAACRFPPEGIRSYGPV
HCCCCEEEEEECCCHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHCCCHHHHHCCCCE
RVGVVYGVAEPAMVARLPFIMPMIETAGALDQLEAIAAVPGIDGLYVGPADLSLSLGLPV
EEEEEECCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCEEECCCCCEEECCCCC
PVDFAAPPFRAALSRVVEVCRRHHLVAGIYASPEWAADLAALGFRFITIVNDSDLISRGA
CCCCCCCCHHHHHHHHHHHHHHHCEEEEEECCCHHHHHHHHHCEEEEEEECCCHHHHHHH
IAALQRVSG
HHHHHHHCC
>Mature Secondary Structure
MVDHLRQRWASDAVIYNAWLTIPSPWSAELLAAAGFPSLTIDLQHGLIDDPTALQILHTI
CCHHHHHHHCCCEEEEEEEEECCCCCCHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHH
DRRCPVFVRLAWNEPAAIMRALDRGAAGIIAPLITGPADAQALVAACRFPPEGIRSYGPV
HCCCCEEEEEECCCHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHCCCHHHHHCCCCE
RVGVVYGVAEPAMVARLPFIMPMIETAGALDQLEAIAAVPGIDGLYVGPADLSLSLGLPV
EEEEEECCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCEEECCCCCEEECCCCC
PVDFAAPPFRAALSRVVEVCRRHHLVAGIYASPEWAADLAALGFRFITIVNDSDLISRGA
CCCCCCCCHHHHHHHHHHHHHHHCEEEEEECCCHHHHHHHHHCEEEEEEECCCHHHHHHH
IAALQRVSG
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA