Click here to switch to the map view.

The map label for this gene is menB [H]

Identifier: 222524965

GI number: 222524965

Start: 2147132

End: 2147917

Strand: Direct

Name: menB [H]

Synonym: Chy400_1700

Alternate gene names: 222524965

Gene position: 2147132-2147917 (Clockwise)

Preceding gene: 222524964

Following gene: 222524966

Centisome position: 40.75

GC content: 61.07

Gene sequence:

>786_bases
ATGGCAGTGTTGACGCTTACCCGTGATGGCCCGATTGCCACCCTTACGCTACAGCGACCAACCGTTCACAATGCCTTCAA
CCCCGATGTGATCGCCGGAATTACTGAGGTCTGCACCGATCTACGCGATGATCCTGCCGTGCGAGTGCTGATCTTGCAGG
GAGAGGGGCCGTCATTCTGTGCCGGCGCCGATCTCAACTGGATGCAGGCCAGTCTCAACTATTCACGTGAAGAGAATCTG
GCCGATGCTTCTCGCCTTGATGCGATGTTCACGGCCCTCGACACACTGCCGCAAGCGGTGGTTGCCAGGGTACACGGGGC
AGCGCTCGGTGGTGGGGTGGGGCTGGTCTGTTGCGCCGACTATGTGGTTGCGGCTGAAGAGGCTGTTTTTGGGTTAACTG
AGGTGCGCCTGGGTCTGCTGCCGGCGGTTATTGCCCGCTTTGTGACCGCGAAAATCGGCTTTGGGCATGCCCGCGCCCTG
TTTGTGACCGGGCGCCGGATCAAGGCAGCGCAGGCGCTGGCGATTGGGCTGGTACACGAGGTGGTACCGGCTGATCAACT
CGATCAGGCTGTTGACCGTGTCGTGCGCGATTTGTTGCAGGCCGGACCACGTGCGATAGCGATGAGTAAAGCGTTGTTGC
AGGTGGTGCGCACCGGTTCACTCGACGAGGTGCGACGGCTGGCAATCGAAGCGATTGCCTCTGCCCGTACCGGTGCCGAG
GGCCAGGCCGGGTTGCGGGCGTTTCTCAATCGTCAGTCACCACCGTGGGCACCAGAGCAGAAATAG

Upstream 100 bases:

>100_bases
ATCCGTGGCCGCACCAGTGCTGACGTTTTGGCCGAGGTGTTAGCCAGCGTGGAATTACCGCTAGAGTAGTGCTGTTTCGC
CTGGAGAAGGAGCAACCACG

Downstream 100 bases:

>100_bases
ACAGGCACCGACCCTGGACTGAAGTTACGCTGGTACGGAAATGGCGCACCGGCTGATGCCTGTACTATGTGAATGATTGA
ATGAGTCAGGCCCCGCGTTT

Product: enoyl-CoA hydratase/isomerase

Products: NA

Alternate protein names: DHNA-CoA synthase [H]

Number of amino acids: Translated: 261; Mature: 260

Protein sequence:

>261_residues
MAVLTLTRDGPIATLTLQRPTVHNAFNPDVIAGITEVCTDLRDDPAVRVLILQGEGPSFCAGADLNWMQASLNYSREENL
ADASRLDAMFTALDTLPQAVVARVHGAALGGGVGLVCCADYVVAAEEAVFGLTEVRLGLLPAVIARFVTAKIGFGHARAL
FVTGRRIKAAQALAIGLVHEVVPADQLDQAVDRVVRDLLQAGPRAIAMSKALLQVVRTGSLDEVRRLAIEAIASARTGAE
GQAGLRAFLNRQSPPWAPEQK

Sequences:

>Translated_261_residues
MAVLTLTRDGPIATLTLQRPTVHNAFNPDVIAGITEVCTDLRDDPAVRVLILQGEGPSFCAGADLNWMQASLNYSREENL
ADASRLDAMFTALDTLPQAVVARVHGAALGGGVGLVCCADYVVAAEEAVFGLTEVRLGLLPAVIARFVTAKIGFGHARAL
FVTGRRIKAAQALAIGLVHEVVPADQLDQAVDRVVRDLLQAGPRAIAMSKALLQVVRTGSLDEVRRLAIEAIASARTGAE
GQAGLRAFLNRQSPPWAPEQK
>Mature_260_residues
AVLTLTRDGPIATLTLQRPTVHNAFNPDVIAGITEVCTDLRDDPAVRVLILQGEGPSFCAGADLNWMQASLNYSREENLA
DASRLDAMFTALDTLPQAVVARVHGAALGGGVGLVCCADYVVAAEEAVFGLTEVRLGLLPAVIARFVTAKIGFGHARALF
VTGRRIKAAQALAIGLVHEVVPADQLDQAVDRVVRDLLQAGPRAIAMSKALLQVVRTGSLDEVRRLAIEAIASARTGAEG
QAGLRAFLNRQSPPWAPEQK

Specific function: Converts o-succinylbenzoyl-CoA (OSB-CoA) to 1,4- dihydroxy-2-naphthoyl-CoA (DHNA-CoA) [H]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=247, Percent_Identity=26.3157894736842, Blast_Score=88, Evalue=8e-18,
Organism=Homo sapiens, GI31542718, Length=255, Percent_Identity=26.6666666666667, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI4502327, Length=257, Percent_Identity=31.1284046692607, Blast_Score=75, Evalue=4e-14,
Organism=Homo sapiens, GI70995211, Length=189, Percent_Identity=29.6296296296296, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI37594469, Length=191, Percent_Identity=26.7015706806283, Blast_Score=74, Evalue=9e-14,
Organism=Homo sapiens, GI37594471, Length=191, Percent_Identity=26.7015706806283, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI221136756, Length=253, Percent_Identity=23.3201581027668, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI221136753, Length=253, Percent_Identity=23.3201581027668, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI221307494, Length=253, Percent_Identity=23.3201581027668, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI4757966, Length=243, Percent_Identity=24.6913580246914, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI51538581, Length=243, Percent_Identity=24.6913580246914, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI25453481, Length=254, Percent_Identity=23.6220472440945, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI51538573, Length=254, Percent_Identity=23.6220472440945, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI68989263, Length=184, Percent_Identity=29.8913043478261, Blast_Score=68, Evalue=9e-12,
Organism=Homo sapiens, GI116235487, Length=250, Percent_Identity=22.8, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1788597, Length=248, Percent_Identity=32.258064516129, Blast_Score=107, Evalue=7e-25,
Organism=Escherichia coli, GI221142681, Length=262, Percent_Identity=31.2977099236641, Blast_Score=98, Evalue=4e-22,
Organism=Escherichia coli, GI1787659, Length=270, Percent_Identity=30, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI87082183, Length=244, Percent_Identity=26.6393442622951, Blast_Score=81, Evalue=6e-17,
Organism=Escherichia coli, GI1787660, Length=246, Percent_Identity=29.6747967479675, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1788682, Length=184, Percent_Identity=31.5217391304348, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1790281, Length=195, Percent_Identity=31.2820512820513, Blast_Score=75, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI25145438, Length=269, Percent_Identity=30.1115241635688, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17540714, Length=205, Percent_Identity=31.219512195122, Blast_Score=97, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI17535521, Length=253, Percent_Identity=24.5059288537549, Blast_Score=92, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17536985, Length=227, Percent_Identity=26.431718061674, Blast_Score=88, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17554946, Length=251, Percent_Identity=26.2948207171315, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17534483, Length=261, Percent_Identity=23.7547892720307, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17560910, Length=258, Percent_Identity=25.5813953488372, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17558304, Length=184, Percent_Identity=28.2608695652174, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI25144157, Length=267, Percent_Identity=25.4681647940075, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI25144160, Length=267, Percent_Identity=25.4681647940075, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24653139, Length=260, Percent_Identity=28.4615384615385, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI20129971, Length=251, Percent_Identity=25.0996015936255, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24653477, Length=251, Percent_Identity=25.0996015936255, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI19920382, Length=180, Percent_Identity=31.1111111111111, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24650670, Length=194, Percent_Identity=31.4432989690722, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI19922422, Length=266, Percent_Identity=21.0526315789474, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376
- InterPro:   IPR010198 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.1.3.36 [H]

Molecular weight: Translated: 27632; Mature: 27501

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVLTLTRDGPIATLTLQRPTVHNAFNPDVIAGITEVCTDLRDDPAVRVLILQGEGPSFC
CEEEEEECCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEECCCCCEE
AGADLNWMQASLNYSREENLADASRLDAMFTALDTLPQAVVARVHGAALGGGVGLVCCAD
CCCCCHHHHHHCCCCHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
YVVAAEEAVFGLTEVRLGLLPAVIARFVTAKIGFGHARALFVTGRRIKAAQALAIGLVHE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHH
VVPADQLDQAVDRVVRDLLQAGPRAIAMSKALLQVVRTGSLDEVRRLAIEAIASARTGAE
HCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCC
GQAGLRAFLNRQSPPWAPEQK
CHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
AVLTLTRDGPIATLTLQRPTVHNAFNPDVIAGITEVCTDLRDDPAVRVLILQGEGPSFC
EEEEEECCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEECCCCCEE
AGADLNWMQASLNYSREENLADASRLDAMFTALDTLPQAVVARVHGAALGGGVGLVCCAD
CCCCCHHHHHHCCCCHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
YVVAAEEAVFGLTEVRLGLLPAVIARFVTAKIGFGHARALFVTGRRIKAAQALAIGLVHE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHH
VVPADQLDQAVDRVVRDLLQAGPRAIAMSKALLQVVRTGSLDEVRRLAIEAIASARTGAE
HCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCC
GQAGLRAFLNRQSPPWAPEQK
CHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1629163; 8566759; 9387221; 9384377 [H]