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The map label for this gene is abgA [C]

Identifier: 222524960

GI number: 222524960

Start: 2140454

End: 2141644

Strand: Direct

Name: abgA [C]

Synonym: Chy400_1695

Alternate gene names: 222524960

Gene position: 2140454-2141644 (Clockwise)

Preceding gene: 222524959

Following gene: 222524962

Centisome position: 40.62

GC content: 57.93

Gene sequence:

>1191_bases
ATGATGCTCGAACGTGCCCAGGCGTTGGCGGATGAAATTATTCGCATCCGCCGTGACATTCATGCCCATCCCGAACTCGG
CTTTCAGGAACATCGTACCGCTGCGCTCGTTGCCGATACGCTCCACGAAATTGGCGGAATTAAGGTTACTACCGGCGTCG
CCAAAACCGGTGTAGTCGGTGAGTTGGGTGATGGTGATGGTCCGGTTATTGCCATCCGCGCCGATATGGATGCGCTCCCG
ATACAGGAAGAGAACCAGGTTGAATATGCTTCAACGAATCCAGGCGTCATGCATGCCTGTGGCCACGATGCCCATACCGC
CATGCTGCTCGGTGCTGCCCACCTGTTGCGTGAGCGCTTTGCGGCTGAACAGTTGCGTGGCCGGGTGCGCTTCCTGTTCC
AGCCGTCTGAAGAGGGTTGGGATGATGAGGTAAAGAGTGGTGGTTTGCGGATGGTTGAAGAGGGTGCATTGGATGGGGTT
GATGCCGTTATTGCCCTGCACGTCGACTCAACCCTGCCCGTTGGTCAGGTGACCATCCGCGGCGGTTGGACCTCGGCTGC
CGTTGATGATTTTAAGGGCTATATCCGCGGTACCGGCGGCCACGGTGCATACCCACATCTCGGCACCGATCCGGTGTTTA
TGCTTTCACACGTTCTGAATGCGCTGTTTGGTATTCGCGCCCGCCTGATCAACCCGATGGAACCGGCTATTCTCAGCGTT
GGCACCGTGCGGGGTGGCCATGCTTCCAACGTTATTCCGAGCGAGATCTTCGTGCAGGGAACCCTGCGTAGCTTCAGCGA
AGAAGTACGCGCTAAACTGGCCCGCGAAGTTGAACGCGCCTTCGCAGTGGCGGAAGCATTTGGCGGGAGCGCTGAAGTCA
AGATCACCCGCGGCTATCCCGCTGGTTGGAACGACGAGCGTGTAGCCGAGTGGATGAGCAATGTTGCCGGTGATTTTCTT
GGCGCTGATGCGATTGATCGTTCGCGCACCGGGATGGGAGCAGAAGATTTTGCCTACATGACGAAAAAGGCACCAGGTGC
CATGCTGATGCTCGGTGCCGCGATCAATGACGGCATCGTGCGCGGGCACCATACCCCCATCTTCGACATTGATGAACGCG
CACTCCCCATTGGCACCGCTATCCTGGCCGAGACCGCTCTCCGCTTTCTGCGCGGAGAAGTGAAACTGTAG

Upstream 100 bases:

>100_bases
TTCGCACGCTGGCAACGTTGCAAGATGGCATTACATATGCACTTGGCCGGCAATCCCTCGGCCAGACCATAGTCCACCGC
ATAAGATAAAGGAGTCGATG

Downstream 100 bases:

>100_bases
TCGACTACTACCAGGGAGGGTAGAGAAACAACCTGCCCTCCCTGATCTTCACCCTGCCAGTCCCCCTAGCTTCTGCCAGT
AACGAGCCAGACCACGCCAG

Product: amidohydrolase

Products: carboxylate; L-amino acid

Alternate protein names: NA

Number of amino acids: Translated: 396; Mature: 396

Protein sequence:

>396_residues
MMLERAQALADEIIRIRRDIHAHPELGFQEHRTAALVADTLHEIGGIKVTTGVAKTGVVGELGDGDGPVIAIRADMDALP
IQEENQVEYASTNPGVMHACGHDAHTAMLLGAAHLLRERFAAEQLRGRVRFLFQPSEEGWDDEVKSGGLRMVEEGALDGV
DAVIALHVDSTLPVGQVTIRGGWTSAAVDDFKGYIRGTGGHGAYPHLGTDPVFMLSHVLNALFGIRARLINPMEPAILSV
GTVRGGHASNVIPSEIFVQGTLRSFSEEVRAKLAREVERAFAVAEAFGGSAEVKITRGYPAGWNDERVAEWMSNVAGDFL
GADAIDRSRTGMGAEDFAYMTKKAPGAMLMLGAAINDGIVRGHHTPIFDIDERALPIGTAILAETALRFLRGEVKL

Sequences:

>Translated_396_residues
MMLERAQALADEIIRIRRDIHAHPELGFQEHRTAALVADTLHEIGGIKVTTGVAKTGVVGELGDGDGPVIAIRADMDALP
IQEENQVEYASTNPGVMHACGHDAHTAMLLGAAHLLRERFAAEQLRGRVRFLFQPSEEGWDDEVKSGGLRMVEEGALDGV
DAVIALHVDSTLPVGQVTIRGGWTSAAVDDFKGYIRGTGGHGAYPHLGTDPVFMLSHVLNALFGIRARLINPMEPAILSV
GTVRGGHASNVIPSEIFVQGTLRSFSEEVRAKLAREVERAFAVAEAFGGSAEVKITRGYPAGWNDERVAEWMSNVAGDFL
GADAIDRSRTGMGAEDFAYMTKKAPGAMLMLGAAINDGIVRGHHTPIFDIDERALPIGTAILAETALRFLRGEVKL
>Mature_396_residues
MMLERAQALADEIIRIRRDIHAHPELGFQEHRTAALVADTLHEIGGIKVTTGVAKTGVVGELGDGDGPVIAIRADMDALP
IQEENQVEYASTNPGVMHACGHDAHTAMLLGAAHLLRERFAAEQLRGRVRFLFQPSEEGWDDEVKSGGLRMVEEGALDGV
DAVIALHVDSTLPVGQVTIRGGWTSAAVDDFKGYIRGTGGHGAYPHLGTDPVFMLSHVLNALFGIRARLINPMEPAILSV
GTVRGGHASNVIPSEIFVQGTLRSFSEEVRAKLAREVERAFAVAEAFGGSAEVKITRGYPAGWNDERVAEWMSNVAGDFL
GADAIDRSRTGMGAEDFAYMTKKAPGAMLMLGAAINDGIVRGHHTPIFDIDERALPIGTAILAETALRFLRGEVKL

Specific function: Required But Not Essential For Aminobenzoyl-Glutamate Utilization. May Participate In Hydrolysis Of Aminobenzoyl- Glutamate To Aminobenzoate, Either Alone Or In Combination With Abgb. [C]

COG id: COG1473

COG function: function code R; Metal-dependent amidase/aminoacylase/carboxypeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20 family [H]

Homologues:

Organism=Escherichia coli, GI87081880, Length=437, Percent_Identity=29.5194508009153, Blast_Score=135, Evalue=4e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017439
- InterPro:   IPR010168
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: 3.5.1.14

Molecular weight: Translated: 42493; Mature: 42493

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMLERAQALADEIIRIRRDIHAHPELGFQEHRTAALVADTLHEIGGIKVTTGVAKTGVVG
CCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCEEEECCCCCCCCEE
ELGDGDGPVIAIRADMDALPIQEENQVEYASTNPGVMHACGHDAHTAMLLGAAHLLRERF
ECCCCCCCEEEEEECCCCCCCCCCCCEEEECCCCCEEEECCCCHHHHHHHHHHHHHHHHH
AAEQLRGRVRFLFQPSEEGWDDEVKSGGLRMVEEGALDGVDAVIALHVDSTLPVGQVTIR
HHHHHCCCEEEEECCCCCCCCHHHHCCCCEEEHHCCCCCCCEEEEEEECCCCCCCEEEEE
GGWTSAAVDDFKGYIRGTGGHGAYPHLGTDPVFMLSHVLNALFGIRARLINPMEPAILSV
CCCCHHHHHHHHHHEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHEEE
GTVRGGHASNVIPSEIFVQGTLRSFSEEVRAKLAREVERAFAVAEAFGGSAEVKITRGYP
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCC
AGWNDERVAEWMSNVAGDFLGADAIDRSRTGMGAEDFAYMTKKAPGAMLMLGAAINDGIV
CCCCHHHHHHHHHHHHHHHHCCCHHCCCCCCCCHHHHHHHHHCCCCEEEEEEEHHCCCEE
RGHHTPIFDIDERALPIGTAILAETALRFLRGEVKL
ECCCCCEEECCCCCCCHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MMLERAQALADEIIRIRRDIHAHPELGFQEHRTAALVADTLHEIGGIKVTTGVAKTGVVG
CCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCEEEECCCCCCCCEE
ELGDGDGPVIAIRADMDALPIQEENQVEYASTNPGVMHACGHDAHTAMLLGAAHLLRERF
ECCCCCCCEEEEEECCCCCCCCCCCCEEEECCCCCEEEECCCCHHHHHHHHHHHHHHHHH
AAEQLRGRVRFLFQPSEEGWDDEVKSGGLRMVEEGALDGVDAVIALHVDSTLPVGQVTIR
HHHHHCCCEEEEECCCCCCCCHHHHCCCCEEEHHCCCCCCCEEEEEEECCCCCCCEEEEE
GGWTSAAVDDFKGYIRGTGGHGAYPHLGTDPVFMLSHVLNALFGIRARLINPMEPAILSV
CCCCHHHHHHHHHHEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHEEE
GTVRGGHASNVIPSEIFVQGTLRSFSEEVRAKLAREVERAFAVAEAFGGSAEVKITRGYP
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCC
AGWNDERVAEWMSNVAGDFLGADAIDRSRTGMGAEDFAYMTKKAPGAMLMLGAAINDGIV
CCCCHHHHHHHHHHHHHHHHCCCHHCCCCCCCCHHHHHHHHHCCCCEEEEEEEHHCCCEE
RGHHTPIFDIDERALPIGTAILAETALRFLRGEVKL
ECCCCCEEECCCCCCCHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: N-acyl-L-amino acid; H2O

Specific reaction: An N-acyl-L-amino acid + H2O = a carboxylate + an L-amino acid

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]