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The map label for this gene is thlA [H]

Identifier: 222524857

GI number: 222524857

Start: 2014125

End: 2015312

Strand: Direct

Name: thlA [H]

Synonym: Chy400_1584

Alternate gene names: 222524857

Gene position: 2014125-2015312 (Clockwise)

Preceding gene: 222524850

Following gene: 222524858

Centisome position: 38.23

GC content: 58.25

Gene sequence:

>1188_bases
ATGAGCGAGAAGCGAGAGGTCGTGGTGCTCAGCGGCGTGCGCACGGCCATTGGCACATTTGGTGGTAGTTTGAAGGATAT
TCCGCCAACCGAATTGGCGGCACTGGTTACCCGTGAAGCAGTTGCCCGCTCTGGCCTGCAACCAAACGAAATCGGTCATG
TGGTCTTCGGGCACGTGATCAATACCGAACCGCATGATATGTATCTGGCTCGCTATGCGGCGGTACGCGGCGGTCTGTCG
GTAGAGACTCCAGCCCTAACGCTTAACCGGCTCTGCGGTAGTGGGTTGCAGGCTATCGTGTCGGCGGCCCAATACATTTT
GCAAGGTGATGCTGAAGCAGCCGTTGCCGGTGGTGCCGAGTGTATGAGCCGTGGCCCGTACAGCTTGCCGGCCATGCGTT
TCGGTGCCCGTATGAATGATTCAAAGGTCGTCGATATGATGGTCGGTGCCCTAACCGACCCGTTTGACGATTGCCATATG
GGGGTAACTGCCGAAAACGTGGCGGCAAAGTGGGGAATCAGCCGCGAAGATCAGGATCAACTGGCTTACGAGAGCCATAT
GCGCGCAGCGCGGGCGATTGACGAAGGACGTTTCGCCAATCAGATCGTGCCGGTTGAGATTAAGGTCAAGGGTGGTACCG
CCCAATTCATGGTTGATGAAGGGGTACGCCGCGATACGACCATCGACAAGCTGGCCAAGCTCCGCCCGGTGTTTCTGAAG
GATGGTTCGGTGACGGCCGGCAATGCTTCGAGCATCAATGATGCAGCAGCGGCGGTAGTGCTGATGGATCGGGCCACCGC
TGAGCGGCGTGGCTACAAGCCGCTGGCGCGTCTGGTCGGTTACAGCCACGCAGCCGTTGAGCCAAAGTATATGGGGATTG
GCCCGGTACCGGCTGTACGACGCCTGCTTGAGCGCACCGGCTTGCGCATCAGTGATATTGATCTTTTCGAGGTCAACGAA
GCTTTTGCAGCGCAGGCGCTGGCTGTGATCCGCGATCTGGAGTTGCCGCCTGATCGCACGAATCCCAATGGTAGTGGTAT
CTCCCTCGGTCACCCGATTGGCGCCACCGGCTGTATTTTGACCGTCAAAGCAATTCACGAACTACACCGCACCGGTGGCC
GTTATGCCCTGGTCACGATGTGTATCGGTGGTGGGCAGGGGATTGCTGCGATCTTCGAGCGAATGTAG

Upstream 100 bases:

>100_bases
CCCATTCCTTGTTGCTAATCCTGATATAATTGTACGTGTTCCTTGAAGTTCGGCAAGTAACTGGCGGAGTCGCCCGGCGA
TCCGTCGCAAGGAGGTCGTC

Downstream 100 bases:

>100_bases
GTGTGCTACAATTGTGAGGCTACGAGCCGGCACCCAGCGTTGGTGTTGCCGGCTCCAGGAACAAAAATCGCACGTTTTGG
AGGTAGGAATGGGCAAGTTA

Product: acetyl-CoA acetyltransferase

Products: NA

Alternate protein names: Acetoacetyl-CoA thiolase [H]

Number of amino acids: Translated: 395; Mature: 394

Protein sequence:

>395_residues
MSEKREVVVLSGVRTAIGTFGGSLKDIPPTELAALVTREAVARSGLQPNEIGHVVFGHVINTEPHDMYLARYAAVRGGLS
VETPALTLNRLCGSGLQAIVSAAQYILQGDAEAAVAGGAECMSRGPYSLPAMRFGARMNDSKVVDMMVGALTDPFDDCHM
GVTAENVAAKWGISREDQDQLAYESHMRAARAIDEGRFANQIVPVEIKVKGGTAQFMVDEGVRRDTTIDKLAKLRPVFLK
DGSVTAGNASSINDAAAAVVLMDRATAERRGYKPLARLVGYSHAAVEPKYMGIGPVPAVRRLLERTGLRISDIDLFEVNE
AFAAQALAVIRDLELPPDRTNPNGSGISLGHPIGATGCILTVKAIHELHRTGGRYALVTMCIGGGQGIAAIFERM

Sequences:

>Translated_395_residues
MSEKREVVVLSGVRTAIGTFGGSLKDIPPTELAALVTREAVARSGLQPNEIGHVVFGHVINTEPHDMYLARYAAVRGGLS
VETPALTLNRLCGSGLQAIVSAAQYILQGDAEAAVAGGAECMSRGPYSLPAMRFGARMNDSKVVDMMVGALTDPFDDCHM
GVTAENVAAKWGISREDQDQLAYESHMRAARAIDEGRFANQIVPVEIKVKGGTAQFMVDEGVRRDTTIDKLAKLRPVFLK
DGSVTAGNASSINDAAAAVVLMDRATAERRGYKPLARLVGYSHAAVEPKYMGIGPVPAVRRLLERTGLRISDIDLFEVNE
AFAAQALAVIRDLELPPDRTNPNGSGISLGHPIGATGCILTVKAIHELHRTGGRYALVTMCIGGGQGIAAIFERM
>Mature_394_residues
SEKREVVVLSGVRTAIGTFGGSLKDIPPTELAALVTREAVARSGLQPNEIGHVVFGHVINTEPHDMYLARYAAVRGGLSV
ETPALTLNRLCGSGLQAIVSAAQYILQGDAEAAVAGGAECMSRGPYSLPAMRFGARMNDSKVVDMMVGALTDPFDDCHMG
VTAENVAAKWGISREDQDQLAYESHMRAARAIDEGRFANQIVPVEIKVKGGTAQFMVDEGVRRDTTIDKLAKLRPVFLKD
GSVTAGNASSINDAAAAVVLMDRATAERRGYKPLARLVGYSHAAVEPKYMGIGPVPAVRRLLERTGLRISDIDLFEVNEA
FAAQALAVIRDLELPPDRTNPNGSGISLGHPIGATGCILTVKAIHELHRTGGRYALVTMCIGGGQGIAAIFERM

Specific function: Unknown

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI167614485, Length=394, Percent_Identity=44.9238578680203, Blast_Score=347, Evalue=8e-96,
Organism=Homo sapiens, GI148539872, Length=391, Percent_Identity=42.9667519181586, Blast_Score=335, Evalue=3e-92,
Organism=Homo sapiens, GI4501853, Length=395, Percent_Identity=41.5189873417722, Blast_Score=271, Evalue=8e-73,
Organism=Homo sapiens, GI4557237, Length=393, Percent_Identity=38.4223918575064, Blast_Score=265, Evalue=4e-71,
Organism=Homo sapiens, GI4504327, Length=425, Percent_Identity=34.3529411764706, Blast_Score=211, Evalue=7e-55,
Organism=Homo sapiens, GI194353979, Length=390, Percent_Identity=31.5384615384615, Blast_Score=165, Evalue=6e-41,
Organism=Escherichia coli, GI87082165, Length=391, Percent_Identity=45.7800511508951, Blast_Score=326, Evalue=2e-90,
Organism=Escherichia coli, GI1787663, Length=400, Percent_Identity=45.5, Blast_Score=310, Evalue=1e-85,
Organism=Escherichia coli, GI1788554, Length=397, Percent_Identity=43.8287153652393, Blast_Score=303, Evalue=2e-83,
Organism=Escherichia coli, GI48994986, Length=399, Percent_Identity=39.0977443609023, Blast_Score=248, Evalue=5e-67,
Organism=Escherichia coli, GI1788683, Length=409, Percent_Identity=32.518337408313, Blast_Score=189, Evalue=3e-49,
Organism=Caenorhabditis elegans, GI133906874, Length=390, Percent_Identity=43.3333333333333, Blast_Score=339, Evalue=1e-93,
Organism=Caenorhabditis elegans, GI25147385, Length=392, Percent_Identity=35.4591836734694, Blast_Score=249, Evalue=1e-66,
Organism=Caenorhabditis elegans, GI17535921, Length=402, Percent_Identity=34.8258706467662, Blast_Score=226, Evalue=1e-59,
Organism=Caenorhabditis elegans, GI17551802, Length=424, Percent_Identity=31.8396226415094, Blast_Score=189, Evalue=2e-48,
Organism=Caenorhabditis elegans, GI17535917, Length=402, Percent_Identity=27.8606965174129, Blast_Score=159, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6325229, Length=398, Percent_Identity=36.9346733668342, Blast_Score=241, Evalue=2e-64,
Organism=Saccharomyces cerevisiae, GI6322031, Length=404, Percent_Identity=37.1287128712871, Blast_Score=224, Evalue=1e-59,
Organism=Drosophila melanogaster, GI24655093, Length=391, Percent_Identity=44.2455242966752, Blast_Score=342, Evalue=2e-94,
Organism=Drosophila melanogaster, GI17648125, Length=391, Percent_Identity=43.2225063938619, Blast_Score=314, Evalue=7e-86,
Organism=Drosophila melanogaster, GI24640423, Length=398, Percent_Identity=36.6834170854271, Blast_Score=244, Evalue=5e-65,
Organism=Drosophila melanogaster, GI17137578, Length=426, Percent_Identity=31.924882629108, Blast_Score=179, Evalue=3e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.9 [H]

Molecular weight: Translated: 42032; Mature: 41901

Theoretical pI: Translated: 7.00; Mature: 7.00

Prosite motif: PS00098 THIOLASE_1 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEKREVVVLSGVRTAIGTFGGSLKDIPPTELAALVTREAVARSGLQPNEIGHVVFGHVI
CCCCCCEEEEECHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEE
NTEPHDMYLARYAAVRGGLSVETPALTLNRLCGSGLQAIVSAAQYILQGDAEAAVAGGAE
CCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHCCCCHHHHCCCHH
CMSRGPYSLPAMRFGARMNDSKVVDMMVGALTDPFDDCHMGVTAENVAAKWGISREDQDQ
HHCCCCCCCCHHHHCCCCCCCHHHHHHHHHHCCCHHHHHCCCCHHHHHHHCCCCCCCHHH
LAYESHMRAARAIDEGRFANQIVPVEIKVKGGTAQFMVDEGVRRDTTIDKLAKLRPVFLK
HHHHHHHHHHHHHHCCCHHCCEEEEEEEECCCCEEEEECCCCCCCCHHHHHHHCCCEEEE
DGSVTAGNASSINDAAAAVVLMDRATAERRGYKPLARLVGYSHAAVEPKYMGIGPVPAVR
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCHHCCCCCCCCCCCHHHHH
RLLERTGLRISDIDLFEVNEAFAAQALAVIRDLELPPDRTNPNGSGISLGHPIGATGCIL
HHHHHCCCEEECCEEEECCHHHHHHHHHHHHHCCCCCCCCCCCCCCEECCCCCCCHHHHH
TVKAIHELHRTGGRYALVTMCIGGGQGIAAIFERM
HHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHCC
>Mature Secondary Structure 
SEKREVVVLSGVRTAIGTFGGSLKDIPPTELAALVTREAVARSGLQPNEIGHVVFGHVI
CCCCCEEEEECHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEE
NTEPHDMYLARYAAVRGGLSVETPALTLNRLCGSGLQAIVSAAQYILQGDAEAAVAGGAE
CCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHCCCCHHHHCCCHH
CMSRGPYSLPAMRFGARMNDSKVVDMMVGALTDPFDDCHMGVTAENVAAKWGISREDQDQ
HHCCCCCCCCHHHHCCCCCCCHHHHHHHHHHCCCHHHHHCCCCHHHHHHHCCCCCCCHHH
LAYESHMRAARAIDEGRFANQIVPVEIKVKGGTAQFMVDEGVRRDTTIDKLAKLRPVFLK
HHHHHHHHHHHHHHCCCHHCCEEEEEEEECCCCEEEEECCCCCCCCHHHHHHHCCCEEEE
DGSVTAGNASSINDAAAAVVLMDRATAERRGYKPLARLVGYSHAAVEPKYMGIGPVPAVR
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCHHCCCCCCCCCCCHHHHH
RLLERTGLRISDIDLFEVNEAFAAQALAVIRDLELPPDRTNPNGSGISLGHPIGATGCIL
HHHHHCCCEEECCEEEECCHHHHHHHHHHHHHCCCCCCCCCCCCCCEECCCCCCCHHHHH
TVKAIHELHRTGGRYALVTMCIGGGQGIAAIFERM
HHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7867955; 11075929; 11466286; 1685080 [H]