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The map label for this gene is cysK [H]

Identifier: 222524736

GI number: 222524736

Start: 1857338

End: 1858267

Strand: Reverse

Name: cysK [H]

Synonym: Chy400_1463

Alternate gene names: 222524736

Gene position: 1858267-1857338 (Counterclockwise)

Preceding gene: 222524738

Following gene: 222524735

Centisome position: 35.27

GC content: 59.03

Gene sequence:

>930_bases
GTGGCCCGTATTTACAACTCGATCACAGAACTGATTGGTAACACGCCGCTGGTGCGGCTTAATCGCGTTAATGATACCCA
GGCTACCGTGGTAGCCAAGCTCGAATTCTTCAACCCGGCCAGCTCGGTCAAAGATCGTATCGGTCTGGCAATGATCGACG
CTGCCGAGCGTGAAGGGTTGATCCGACCCGGCGAGACGGTCATTATCGAGCCGACAAGCGGCAATACCGGGATCGGTCTG
GCGTTAGTCGCTGCGGCACGTGGTTACCGGCTAATCCTGACGATGCCGGAAACGATGAGCATCGAACGGCGCAAACTGTT
GCGTGGCTTTGGCGCCGAGCTGGTGTTGACGCCGGGTAGCGAAGGGATGCGGGGAGCCATTGCTCGCGCCGAGGAGTTGC
TGGCCGAAATCCCCAATAGCTTTATGCCGCAGCAGTTCAAGAACCCGGCCAACCCGGCCATTCACCGTGCCACTACCGCC
GAAGAAATCTGGCGCGATACTGACGGTCAGGTCGATATTGTGGTCGGTGGTGTCGGTACCGGTGGCACACTCACCGGCAT
TGGCGAAGTGCTCAAACCGCGCCGACCGAGTCTGCGCATTGTCGCAGTCGAGCCAGAGGCTTCGCCGGTGTTGTCGGGTG
GCAAGCCTGGGCCGCACAAGATTCAAGGCATTGGCGCCGGGTTTATCCCAGATGTGCTGAATACCAGCATTCTTGACGAG
ATTCAGCAGGTGAGCAACGAAGATGCCTTTGCGATGGCCCGTCGGCTGGCTCGCGAAGAGGGTTTGATGGTCGGTATCTC
AAGTGGGGCTGCGGCGACCGCAGCGCTGCGCGTGGCCCGCCGTCCGGAGAATGCCGGTAAGCTGATCGTCTTTATTGTGC
CCTCAAACGGTGAACGTTATCTATCAACGCCACTCTTCGCTGAAGAGTGA

Upstream 100 bases:

>100_bases
CCACAAAACTTGACTTTTTTGCCTGACAAATTGACATTTATTGCTGCTCTGAGTACAATAGCAGATAGAACGTAAATAGT
TTAGCGTAAAGGAGCCAACT

Downstream 100 bases:

>100_bases
TCAGCCGCCGGAACAACGACCGATTGCCCCAACCTTATAGTTGGGGCAATTTTACTACTCGCATGAAACGCATCTACATC
TGTCTTGGGCCACATTGCCG

Product: cysteine synthase A

Products: NA

Alternate protein names: OAS sulfhydrylase; OASS; Cysteine synthase A; CSase A; O-acetylserine (thiol)-lyase A; OAS-TL A; O-acetylserine-specific cysteine synthase; Sulfide-dependent cysteine synthase [H]

Number of amino acids: Translated: 309; Mature: 308

Protein sequence:

>309_residues
MARIYNSITELIGNTPLVRLNRVNDTQATVVAKLEFFNPASSVKDRIGLAMIDAAEREGLIRPGETVIIEPTSGNTGIGL
ALVAAARGYRLILTMPETMSIERRKLLRGFGAELVLTPGSEGMRGAIARAEELLAEIPNSFMPQQFKNPANPAIHRATTA
EEIWRDTDGQVDIVVGGVGTGGTLTGIGEVLKPRRPSLRIVAVEPEASPVLSGGKPGPHKIQGIGAGFIPDVLNTSILDE
IQQVSNEDAFAMARRLAREEGLMVGISSGAAATAALRVARRPENAGKLIVFIVPSNGERYLSTPLFAEE

Sequences:

>Translated_309_residues
MARIYNSITELIGNTPLVRLNRVNDTQATVVAKLEFFNPASSVKDRIGLAMIDAAEREGLIRPGETVIIEPTSGNTGIGL
ALVAAARGYRLILTMPETMSIERRKLLRGFGAELVLTPGSEGMRGAIARAEELLAEIPNSFMPQQFKNPANPAIHRATTA
EEIWRDTDGQVDIVVGGVGTGGTLTGIGEVLKPRRPSLRIVAVEPEASPVLSGGKPGPHKIQGIGAGFIPDVLNTSILDE
IQQVSNEDAFAMARRLAREEGLMVGISSGAAATAALRVARRPENAGKLIVFIVPSNGERYLSTPLFAEE
>Mature_308_residues
ARIYNSITELIGNTPLVRLNRVNDTQATVVAKLEFFNPASSVKDRIGLAMIDAAEREGLIRPGETVIIEPTSGNTGIGLA
LVAAARGYRLILTMPETMSIERRKLLRGFGAELVLTPGSEGMRGAIARAEELLAEIPNSFMPQQFKNPANPAIHRATTAE
EIWRDTDGQVDIVVGGVGTGGTLTGIGEVLKPRRPSLRIVAVEPEASPVLSGGKPGPHKIQGIGAGFIPDVLNTSILDEI
QQVSNEDAFAMARRLAREEGLMVGISSGAAATAALRVARRPENAGKLIVFIVPSNGERYLSTPLFAEE

Specific function: Catalyzes the conversion of O-acetylserine (OAS) to cysteine through the elimination of acetate and addition of hydrogen sulfide [H]

COG id: COG0031

COG function: function code E; Cysteine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cysteine synthase/cystathionine beta- synthase family [H]

Homologues:

Organism=Homo sapiens, GI295821202, Length=311, Percent_Identity=43.4083601286174, Blast_Score=250, Evalue=1e-66,
Organism=Homo sapiens, GI295821200, Length=311, Percent_Identity=43.4083601286174, Blast_Score=250, Evalue=1e-66,
Organism=Homo sapiens, GI4557415, Length=311, Percent_Identity=43.4083601286174, Blast_Score=250, Evalue=1e-66,
Organism=Escherichia coli, GI1788754, Length=317, Percent_Identity=55.8359621451104, Blast_Score=309, Evalue=2e-85,
Organism=Escherichia coli, GI2367138, Length=305, Percent_Identity=42.6229508196721, Blast_Score=228, Evalue=5e-61,
Organism=Caenorhabditis elegans, GI17535051, Length=300, Percent_Identity=56.3333333333333, Blast_Score=347, Evalue=4e-96,
Organism=Caenorhabditis elegans, GI115535073, Length=308, Percent_Identity=56.4935064935065, Blast_Score=317, Evalue=4e-87,
Organism=Caenorhabditis elegans, GI17562970, Length=300, Percent_Identity=51.6666666666667, Blast_Score=310, Evalue=4e-85,
Organism=Caenorhabditis elegans, GI32566674, Length=293, Percent_Identity=52.5597269624573, Blast_Score=304, Evalue=3e-83,
Organism=Caenorhabditis elegans, GI17561720, Length=299, Percent_Identity=50.1672240802676, Blast_Score=275, Evalue=2e-74,
Organism=Caenorhabditis elegans, GI32566672, Length=214, Percent_Identity=52.3364485981308, Blast_Score=226, Evalue=9e-60,
Organism=Caenorhabditis elegans, GI17534315, Length=316, Percent_Identity=40.8227848101266, Blast_Score=196, Evalue=1e-50,
Organism=Caenorhabditis elegans, GI25147552, Length=319, Percent_Identity=36.9905956112853, Blast_Score=179, Evalue=2e-45,
Organism=Caenorhabditis elegans, GI17561716, Length=117, Percent_Identity=51.2820512820513, Blast_Score=112, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6321594, Length=315, Percent_Identity=37.4603174603175, Blast_Score=205, Evalue=9e-54,
Organism=Saccharomyces cerevisiae, GI6321449, Length=325, Percent_Identity=34.7692307692308, Blast_Score=130, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24643623, Length=311, Percent_Identity=42.1221864951768, Blast_Score=218, Evalue=6e-57,
Organism=Drosophila melanogaster, GI20129101, Length=311, Percent_Identity=42.1221864951768, Blast_Score=218, Evalue=6e-57,

Paralogues:

None

Copy number: 2820 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001216
- InterPro:   IPR005856
- InterPro:   IPR005859
- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =2.5.1.47 [H]

Molecular weight: Translated: 32907; Mature: 32776

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: PS00901 CYS_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARIYNSITELIGNTPLVRLNRVNDTQATVVAKLEFFNPASSVKDRIGLAMIDAAEREGL
CCHHHHHHHHHHCCCCEEEEECCCCCCEEEEEEEEECCCHHHHHHHHCEEEEECHHHCCC
IRPGETVIIEPTSGNTGIGLALVAAARGYRLILTMPETMSIERRKLLRGFGAELVLTPGS
CCCCCEEEEEECCCCCCHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHCCCEEEECCCC
EGMRGAIARAEELLAEIPNSFMPQQFKNPANPAIHRATTAEEIWRDTDGQVDIVVGGVGT
CCHHHHHHHHHHHHHHCCHHCCCHHHCCCCCCHHHHCCCHHHHHCCCCCEEEEEEECCCC
GGTLTGIGEVLKPRRPSLRIVAVEPEASPVLSGGKPGPHKIQGIGAGFIPDVLNTSILDE
CCCHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHH
IQQVSNEDAFAMARRLAREEGLMVGISSGAAATAALRVARRPENAGKLIVFIVPSNGERY
HHHHCCCHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHCCCCCCCCEEEEEECCCCCEE
LSTPLFAEE
ECCCCCCCC
>Mature Secondary Structure 
ARIYNSITELIGNTPLVRLNRVNDTQATVVAKLEFFNPASSVKDRIGLAMIDAAEREGL
CHHHHHHHHHHCCCCEEEEECCCCCCEEEEEEEEECCCHHHHHHHHCEEEEECHHHCCC
IRPGETVIIEPTSGNTGIGLALVAAARGYRLILTMPETMSIERRKLLRGFGAELVLTPGS
CCCCCEEEEEECCCCCCHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHCCCEEEECCCC
EGMRGAIARAEELLAEIPNSFMPQQFKNPANPAIHRATTAEEIWRDTDGQVDIVVGGVGT
CCHHHHHHHHHHHHHHCCHHCCCHHHCCCCCCHHHHCCCHHHHHCCCCCEEEEEEECCCC
GGTLTGIGEVLKPRRPSLRIVAVEPEASPVLSGGKPGPHKIQGIGAGFIPDVLNTSILDE
CCCHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHH
IQQVSNEDAFAMARRLAREEGLMVGISSGAAATAALRVARRPENAGKLIVFIVPSNGERY
HHHHCCCHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHCCCCCCCCEEEEEECCCCCEE
LSTPLFAEE
ECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]